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Q66JG3

- SYEM_XENTR

UniProt

Q66JG3 - SYEM_XENTR

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Protein

Probable glutamate--tRNA ligase, mitochondrial

Gene
ears2
Organism
Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity.UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei48 – 481ATP By similarity
Binding sitei74 – 741Glutamate By similarity
Binding sitei244 – 2441Glutamate By similarity
Binding sitei247 – 2471ATP By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi282 – 2865ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. glutamate-tRNA ligase activity Source: UniProtKB-EC
  3. tRNA binding Source: InterPro

GO - Biological processi

  1. glutamyl-tRNA aminoacylation Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable glutamate--tRNA ligase, mitochondrial (EC:6.1.1.17)
Alternative name(s):
Glutamyl-tRNA synthetase
Short name:
GluRS
Gene namesi
Name:ears2
OrganismiXenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Taxonomic identifieri8364 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusSilurana
ProteomesiUP000008143: Unplaced

Organism-specific databases

XenbaseiXB-GENE-970848. ears2.

Subcellular locationi

Mitochondrion matrix By similarity UniRule annotation

GO - Cellular componenti

  1. mitochondrial matrix Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3939Mitochondrion Reviewed predictionAdd
BLAST
Chaini40 – 516477Probable glutamate--tRNA ligase, mitochondrialUniRule annotationPRO_0000254565Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi8364.ENSXETP00000027183.

Structurei

3D structure databases

ProteinModelPortaliQ66JG3.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni38 – 403Glutamate binding By similarity
Regioni226 – 2305Glutamate binding By similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi43 – 519"HIGH" regionUniRule annotation
Motifi282 – 2865"KMSKS" regionUniRule annotation

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000252720.
HOVERGENiHBG056174.
InParanoidiQ66JG3.
KOiK01885.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q66JG3-1 [UniParc]FASTAAdd to Basket

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MRPAFIRGKW LSRTLELATG LGRRTCSSRE SGREVRVRFA PSPTGFLHLG    50
GLRTALYNYL FAKKHGGAFI LRLEDTDRSR LVPGAAESIE DMLEWAGIPP 100
DESPRHGGPC GPYEQSKRLD LYHVAAQALL DSGAAYRCFC TPQRLELLKR 150
EAMRNRQTPR YDNRCRHLTP KQVEEKLSRN SPFVIRFFLQ EGAQPFQDLV 200
YGWTQHDVAS VEGDPVILKG DGFPTYHLAN VVDDHHMCVS HVLRGAEWLI 250
STAKHLLLYQ ALGWQPPQFA HLPLLLNKDG SKLSKRQGDI FIQHYVHSGY 300
LSDALLDLIT NCGSGFTENQ MGRTVDTLIQ QYELGKTSTH SALLDLDKLP 350
EFNRIHLTRW IEGTETRVQL VGQLQVLLKD TYKDLELDEK HIERILLLRK 400
GHLCRLTDLL SPEYSYLWVR PSVTREQLQC LTSEASKVKN LVVRLLQEND 450
SGFTLETLNG ELRKQLKQVK DTKYSSAMKL LRVALSGQEH GPSVAEMLLS 500
LGRQESIVRL QNALPD 516
Length:516
Mass (Da):58,607
Last modified:October 11, 2004 - v1
Checksum:iEB54FFDEBE953600
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC080925 mRNA. Translation: AAH80925.1.
RefSeqiNP_001008042.1. NM_001008041.1.
UniGeneiStr.26785.

Genome annotation databases

GeneIDi493404.
KEGGixtr:493404.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC080925 mRNA. Translation: AAH80925.1 .
RefSeqi NP_001008042.1. NM_001008041.1.
UniGenei Str.26785.

3D structure databases

ProteinModelPortali Q66JG3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 8364.ENSXETP00000027183.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 493404.
KEGGi xtr:493404.

Organism-specific databases

CTDi 124454.
Xenbasei XB-GENE-970848. ears2.

Phylogenomic databases

eggNOGi COG0008.
HOGENOMi HOG000252720.
HOVERGENi HBG056174.
InParanoidi Q66JG3.
KOi K01885.

Family and domain databases

Gene3Di 1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPi MF_00022_B. Glu_tRNA_synth_B.
InterProi IPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view ]
PANTHERi PTHR10119. PTHR10119. 1 hit.
Pfami PF00749. tRNA-synt_1c. 1 hit.
[Graphical view ]
PRINTSi PR00987. TRNASYNTHGLU.
SUPFAMi SSF48163. SSF48163. 1 hit.
TIGRFAMsi TIGR00464. gltX_bact. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. NIH - Xenopus Gene Collection (XGC) project
    Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Embryo.

Entry informationi

Entry nameiSYEM_XENTR
AccessioniPrimary (citable) accession number: Q66JG3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: October 11, 2004
Last modified: February 19, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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