Q66IJ4 (PIGM_XENTR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GPI mannosyltransferase 1
EC=2.4.1.-

Alternative name(s):
GPI mannosyltransferase I
Short name=GPI-MT-I
Phosphatidylinositol-glycan biosynthesis class M protein
Short name=PIG-M

Gene names

Name:	pigm
ORF Names:	TGas014d02.1

Organism

Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)

Taxonomic identifier

8364 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Silurana

Protein attributes

Sequence length	419 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Mannosyltransferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers the first alpha-1,4-mannose to GlcN-acyl-PI during GPI precursor assembly By similarity.
Pathway	Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the PIGM family.

Ontologies

Keywords
Biological process	GPI-anchor biosynthesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	GPI anchor biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	transferase activity, transferring hexosyl groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 419	419	GPI mannosyltransferase 1		PRO_0000246220
Regions
	Topological domain	1 – 13	13	Cytoplasmic Potential
	Transmembrane	14 – 34	21	Helical; Potential
	Topological domain	35 – 88	54	Lumenal Potential
	Transmembrane	89 – 111	23	Helical; Potential
	Topological domain	112 – 114	3	Cytoplasmic Potential
	Transmembrane	115 – 135	21	Helical; Potential
	Topological domain	136 – 168	33	Lumenal Potential
	Transmembrane	169 – 189	21	Helical; Potential
	Topological domain	190 – 224	35	Cytoplasmic Potential
	Transmembrane	225 – 245	21	Helical; Potential
	Topological domain	246 – 285	40	Lumenal Potential
	Transmembrane	286 – 306	21	Helical; Potential
	Topological domain	307 – 327	21	Cytoplasmic Potential
	Transmembrane	328 – 348	21	Helical; Potential
	Topological domain	349 – 355	7	Lumenal Potential
	Transmembrane	356 – 376	21	Helical; Potential
	Topological domain	377 – 382	6	Cytoplasmic Potential
	Transmembrane	383 – 403	21	Helical; Potential
	Topological domain	404 – 419	16	Lumenal Potential

Sequences

	Sequence		Length	Mass (Da)
	Q66IJ4 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: ECBD805202269343	FASTA	419	49,036
	10 20 30 40 50 60 MMKQAGILKI FLQHQFMFPT AFFLRSALVL FGVYQDQTML VKYTDVDYHV FTDAAEYLTQ 70 80 90 100 110 120 GVSPYKRATY RYTPLLAWIL TPNIYVTELY GKMLFVCCDL LAAYLIHRIL VDRGIKDSAS 130 140 150 160 170 180 LYCAIWLFNP LPMVVSSRGN AESVLAVLVL SVLYYVQKRR LIKGALIYGL SVHMKIYPIT 190 200 210 220 230 240 YILPIALFFQ KEDFYGSQEG KRVVSNLKYI RIFRNLLQRL LSRDILLFVT VSGVTFALLT 250 260 270 280 290 300 LFFYYRYGWE FLENTYLYHL TRRDIRHNFS PYFYMLYLTA ENNNSFILGL AAFFPQLVLL 310 320 330 340 350 360 FVVSLAYFKD LPFCCFLHTA IFVSFNKVCT SQYFLWYLCL LPLVMPGLKM SMTNGICLII 370 380 390 400 410 LWFFSQAIWL VPAYFLEFEG QNTFLYIWCA GLLFLLINTV IIVQIISNYQ LQSKKTKKT

References

[1]	Sanger Xenopus tropicalis EST/cDNA project Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Gastrula.
[2]	NIH - Xenopus Gene Collection (XGC) project Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryo.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR760728 mRNA. Translation: CAJ83771.1. BC081324 mRNA. Translation: AAH81324.1.
RefSeq	NP_001008120.1. NM_001008119.1.
UniGene	Str.27175.
3D structure databases
ProteinModelPortal	Q66IJ4.
ModBase	Search...
Protein-protein interaction databases
STRING	Q66IJ4.
Protein family/group databases
CAZy	GT50. Glycosyltransferase Family 50.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSXETT00000013605; ENSXETP00000013605; ENSXETG00000006188.
GeneID	493482.
KEGG	xtr:493482.
Organism-specific databases
CTD	93183.
Xenbase	XB-GENE-5730241. pigm.
Phylogenomic databases
eggNOG	veNOG06486.
GeneTree	ENSGT00390000017728.
HOGENOM	HBG559139.
HOVERGEN	HBG082137.
InParanoid	Q66IJ4.
OMA	EFLEHTY.
OrthoDB	EOG4Q2DG6.
Gene expression databases
Bgee	Q66IJ4.
Family and domain databases
InterPro	IPR007704. Mannosyltransferase_DXD. [Graphical view]
KO	K05284.
PANTHER	PTHR12886. Mannos_trans_DXD. 1 hit.
Pfam	PF05007. Mannosyl_trans. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PIGM_XENTR

Accession

Primary (citable) accession number: Q66IJ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	October 11, 2004
Last modified:	November 16, 2011
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program