Reviewed, UniProtKB/Swiss-Prot Q66I59 (ASPD_DANRE)

Names and origin

Protein names

Recommended name:
    Putative L-aspartate dehydrogenase
    EC=1.4.1.21

Gene names

ORF Names:

zgc:103722

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	276 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Specifically catalyzes the NAD or NADP-dependent dehydrogenation of L-aspartate to iminoaspartate By similarity.
Catalytic activity	L-aspartate + H(2)O + NAD(P)(+) = oxaloacetate + NH(3) + NAD(P)H.
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; iminoaspartate from L-aspartate (dehydrogenase route): step 1/1.
Miscellaneous	The iminoaspartate product is unstable in aqueous solution and can decompose to oxaloacetate and ammonia By similarity.
Sequence similarities	Belongs to the L-aspartate dehydrogenase family.

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis
Ligand	NAD NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro NADP catabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	aspartate dehydrogenase activity Inferred from electronic annotation. Source: InterPro binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 276	276	Putative L-aspartate dehydrogenase		PRO_0000144903
Sites
	Binding site	126	1	NAD; via amide nitrogen By similarity
	Binding site	192	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q66I59-1 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 8DEC2D2AEB852CA9	FASTA	276	29,753
	10 20 30 40 50 60 MADKALKVGI VGYGHLGQFL VEKIQSEGAE VGLQLAFVWN RNADKLKDSL PKDLILHDLS 70 80 90 100 110 120 DFTQRDTDVI VEVCHPLIVK EFGVRFLSHA HFLVGSPSAL SDGQLEQDLR TAAKQQGKTL 130 140 150 160 170 180 YVPSGALWGG QDIQKMNDSG TLRALSIRMS KHPSCFRLTG GLLSDWTEGE GRRVLYRGSV 190 200 210 220 230 240 AELCPLAPNN VNTMAAAAIA ASKLGFHGVT GEIVSDTALA DYHVVEVDVT GPDGFSVKTM 250 260 270 RQNPAKLGAV TGKATYNSFW SSLLVCKGHG GRVYLC

References

[1]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Liver.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC081519 mRNA. Translation: AAH81519.1.
RefSeq	NP_001004657.1.
UniGene	Dr.87480
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSDARG00000038297. Danio rerio. [Contig view]
GeneID	447919.
KEGG	dre:447919.
Organism-specific databases
ZFIN	ZDB-GENE-040912-102. zgc:103722.
Phylogenomic databases
HOGENOM	Q66I59.
HOVERGEN	Q66I59.
Family and domain databases
InterPro	IPR005106. Asp/hSer_DHase_NAD-bd. IPR002811. Asp_DHase. IPR011182. Asp_DHase_NAD_syn. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01958. DUF108. 1 hit. PF03447. NAD_binding_3. 1 hit. [Graphical view]
PIRSF	PIRSF005227. Asp_dh_NAD_syn. 1 hit.
ProDom	PD017325. Asp_dh. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

ASPD_DANRE

Accession

Primary (citable) accession number: Q66I59

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	October 11, 2004
Last modified:	November 25, 2008
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Zebrafish annotation project