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Protein

Argininosuccinate synthase

Gene

ass1

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

One of the enzymes of the urea cycle, the metabolic pathway transforming neurotoxic amonia produced by protein catabolism into inocuous urea in the liver of ureotelic animals. Catalyzes the formation of arginosuccinate from aspartate, citrulline and ATP and together with ASL it is responsible for the biosynthesis of arginine in most body tissues.By similarity

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.By similarity

Pathwayi: L-arginine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.By similarity
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Argininosuccinate synthase (ass1)
  3. no protein annotated in this organism
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Pathwayi: urea cycle

This protein is involved in step 1 of the subpathway that synthesizes (N(omega)-L-arginino)succinate from L-aspartate and L-citrulline.By similarity
Proteins known to be involved in this subpathway in this organism are:
  1. Argininosuccinate synthase (ass1)
This subpathway is part of the pathway urea cycle, which is itself part of Nitrogen metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (N(omega)-L-arginino)succinate from L-aspartate and L-citrulline, the pathway urea cycle and in Nitrogen metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei35ATP; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei86CitrullineBy similarity1
Binding sitei91CitrullineBy similarity1
Binding sitei118AspartateBy similarity1
Binding sitei122AspartateBy similarity1
Binding sitei122CitrullineBy similarity1
Binding sitei123AspartateBy similarity1
Binding sitei126CitrullineBy similarity1
Binding sitei179CitrullineBy similarity1
Binding sitei188CitrullineBy similarity1
Binding sitei269CitrullineBy similarity1
Binding sitei281CitrullineBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi9 – 17ATPBy similarity9
Nucleotide bindingi114 – 122ATPBy similarity9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processAmino-acid biosynthesis, Arginine biosynthesis, Urea cycle
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.
UPA00158; UER00272.

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthaseCurated (EC:6.3.4.5By similarity)
Alternative name(s):
Citrulline--aspartate ligase
Gene namesi
Name:ass1By similarity
ORF Names:zgc:92051Imported
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Unplaced

Organism-specific databases

ZFINiZDB-GENE-040912-178. ass1.

Subcellular locationi

  • Cytoplasmcytosol By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003213231 – 414Argininosuccinate synthaseAdd BLAST414

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ66I24.
PRIDEiQ66I24.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi7955.ENSDARP00000039902.

Structurei

3D structure databases

ProteinModelPortaliQ66I24.
SMRiQ66I24.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the argininosuccinate synthase family.Curated

Phylogenomic databases

eggNOGiKOG1706. Eukaryota.
COG0137. LUCA.
HOGENOMiHOG000230093.
HOVERGENiHBG001717.
InParanoidiQ66I24.
KOiK01940.
PhylomeDBiQ66I24.

Family and domain databases

CDDicd01999. Argininosuccinate_Synthase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1. 1 hit.
InterProiView protein in InterPro
IPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
PANTHERiPTHR11587. PTHR11587. 1 hit.
PfamiView protein in Pfam
PF00764. Arginosuc_synth. 1 hit.
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiView protein in PROSITE
PS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q66I24-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKGLVVLAY SGGLDTSCIL VWLKEQGYDV IAYLANIGQD EDFDVARKKA
60 70 80 90 100
EKLGAKKVFI EDLRSEFVQE FIWPALQANA LYEDRYLLGT SIARPCIARR
110 120 130 140 150
QVQIAQREGA QYVSHGATGK GNDQVRFELT CYALYPQVQV IAPWRIPEFY
160 170 180 190 200
NRFRGRKDLM EYAEKHNIPV PVTPKAPWSM DANLMHISYE SGILENPKNH
210 220 230 240 250
APSDLYQMTK NPEHSPDQAD MLEIEFKKGV PVRVSHLKDG ISKETPLEIF
260 270 280 290 300
CYLNEIGGKH GVGRIDIVEN RFIGMKSRGI YETPGGTVLL QAHLDIETFT
310 320 330 340 350
MDREVRKIKQ SLGIKFSELI YNGFWFSPEC EFVRECVERS QKHVEGRVQL
360 370 380 390 400
SVYKGQVYIL GRESPKSLYN EELVSMDVQG DYDPCDASGF IKINAVRLRE
410
HNRLQGAALS KHNV
Length:414
Mass (Da):47,129
Last modified:October 11, 2004 - v1
Checksum:iA75FD4D9CD0611D3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC081578 mRNA. Translation: AAH81578.1.
BC154282 mRNA. Translation: AAI54283.1.
RefSeqiNP_001004603.1. NM_001004603.1.
UniGeneiDr.4095.

Genome annotation databases

GeneIDi447864.
KEGGidre:447864.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiASSY_DANRE
AccessioniPrimary (citable) accession number: Q66I24
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: October 11, 2004
Last modified: June 7, 2017
This is version 89 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families