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Protein

Argininosuccinate synthase

Gene

ass1

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.

Pathway: L-arginine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Argininosuccinate synthase (ass1)
  3. no protein annotated in this organism
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Pathway: urea cycle

This protein is involved in step 1 of the subpathway that synthesizes (N(omega)-L-arginino)succinate from L-aspartate and L-citrulline.
Proteins known to be involved in this subpathway in this organism are:
  1. Argininosuccinate synthase (ass1)
This subpathway is part of the pathway urea cycle, which is itself part of Nitrogen metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (N(omega)-L-arginino)succinate from L-aspartate and L-citrulline, the pathway urea cycle and in Nitrogen metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei35 – 351ATP; via amide nitrogen and carbonyl oxygenBy similarity
Binding sitei86 – 861CitrullineBy similarity
Binding sitei91 – 911CitrullineBy similarity
Binding sitei118 – 1181AspartateBy similarity
Binding sitei122 – 1221AspartateBy similarity
Binding sitei122 – 1221CitrullineBy similarity
Binding sitei123 – 1231AspartateBy similarity
Binding sitei126 – 1261CitrullineBy similarity
Binding sitei179 – 1791CitrullineBy similarity
Binding sitei188 – 1881CitrullineBy similarity
Binding sitei269 – 2691CitrullineBy similarity
Binding sitei281 – 2811CitrullineBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi9 – 179ATPBy similarity
Nucleotide bindingi114 – 1229ATPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis, Urea cycle

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.
UPA00158; UER00272.

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthase (EC:6.3.4.5)
Alternative name(s):
Citrulline--aspartate ligase
Gene namesi
Name:ass1
Synonyms:ass
ORF Names:zgc:92051
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
ProteomesiUP000000437 Componenti: Unplaced

Organism-specific databases

ZFINiZDB-GENE-040912-178. ass1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 414414Argininosuccinate synthasePRO_0000321323Add
BLAST

Proteomic databases

PRIDEiQ66I24.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi7955.ENSDARP00000039902.

Structurei

3D structure databases

ProteinModelPortaliQ66I24.
SMRiQ66I24. Positions 3-405.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the argininosuccinate synthase family.Curated

Phylogenomic databases

eggNOGiCOG0137.
HOGENOMiHOG000230093.
HOVERGENiHBG001717.
InParanoidiQ66I24.
KOiK01940.
PhylomeDBiQ66I24.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q66I24-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKGLVVLAY SGGLDTSCIL VWLKEQGYDV IAYLANIGQD EDFDVARKKA
60 70 80 90 100
EKLGAKKVFI EDLRSEFVQE FIWPALQANA LYEDRYLLGT SIARPCIARR
110 120 130 140 150
QVQIAQREGA QYVSHGATGK GNDQVRFELT CYALYPQVQV IAPWRIPEFY
160 170 180 190 200
NRFRGRKDLM EYAEKHNIPV PVTPKAPWSM DANLMHISYE SGILENPKNH
210 220 230 240 250
APSDLYQMTK NPEHSPDQAD MLEIEFKKGV PVRVSHLKDG ISKETPLEIF
260 270 280 290 300
CYLNEIGGKH GVGRIDIVEN RFIGMKSRGI YETPGGTVLL QAHLDIETFT
310 320 330 340 350
MDREVRKIKQ SLGIKFSELI YNGFWFSPEC EFVRECVERS QKHVEGRVQL
360 370 380 390 400
SVYKGQVYIL GRESPKSLYN EELVSMDVQG DYDPCDASGF IKINAVRLRE
410
HNRLQGAALS KHNV
Length:414
Mass (Da):47,129
Last modified:October 11, 2004 - v1
Checksum:iA75FD4D9CD0611D3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC081578 mRNA. Translation: AAH81578.1.
BC154282 mRNA. Translation: AAI54283.1.
RefSeqiNP_001004603.1. NM_001004603.1.
UniGeneiDr.4095.

Genome annotation databases

GeneIDi447864.
KEGGidre:447864.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC081578 mRNA. Translation: AAH81578.1.
BC154282 mRNA. Translation: AAI54283.1.
RefSeqiNP_001004603.1. NM_001004603.1.
UniGeneiDr.4095.

3D structure databases

ProteinModelPortaliQ66I24.
SMRiQ66I24. Positions 3-405.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7955.ENSDARP00000039902.

Proteomic databases

PRIDEiQ66I24.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi447864.
KEGGidre:447864.

Organism-specific databases

CTDi445.
ZFINiZDB-GENE-040912-178. ass1.

Phylogenomic databases

eggNOGiCOG0137.
HOGENOMiHOG000230093.
HOVERGENiHBG001717.
InParanoidiQ66I24.
KOiK01940.
PhylomeDBiQ66I24.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.
UPA00158; UER00272.

Miscellaneous databases

NextBioi20832385.
PROiQ66I24.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. NIH - Zebrafish Gene Collection (ZGC) project
    Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Entry informationi

Entry nameiASSY_DANRE
AccessioniPrimary (citable) accession number: Q66I24
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: October 11, 2004
Last modified: April 29, 2015
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.