Reviewed, UniProtKB/Swiss-Prot Q66I21 (AL8A1_DANRE)

Names and origin

Protein names

Recommended name:
    Aldehyde dehydrogenase family 8 member A1
    EC=1.2.1.-

Gene names

Name:

aldh8a1

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	487 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Converts 9-cis-retinal to 9-cis-retinoic acid. Has lower activity towards 13-cis-retinal. Has much lower activity towards all-trans-retinal. Has highest activity with benzaldehyde and decanal (in vitro). Has a preference for NAD, but shows considerable activity with NADP (in vitro) By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the aldehyde dehydrogenase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	oxidoreductase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 487	487	Aldehyde dehydrogenase family 8 member A1		PRO_0000312956
Regions
	Nucleotide binding	231 – 236	6	NAD By similarity
Sites
	Active site	253	1	Proton acceptor By similarity
	Active site	287	1	Nucleophile By similarity
	Site	155	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q66I21-1 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 4F13284B613DE146	FASTA	487	53,354
	10 20 30 40 50 60 MSKDMKYLVL ENYIGGKFVP CSKLIDSFDP STGEVYCKVP DSGAEEVNAA VRAAKEAFPD 70 80 90 100 110 120 WSAKSPADRS KVLNKLADLI EARLEEFVQA ESKDQGKTIT FARNVDIPRS AYNFRFFASS 130 140 150 160 170 180 VLHHTNDCSQ MDHMGCLNYT IRCPVGVAGL ISPWNLPLYL LTWKIAPAVA TGNTVVAKPS 190 200 210 220 230 240 EMTSVTAWMM CQLLEEAGFP PGVVNIVFGT GPRAGDALVS HPDVPLISFT GSTATARLIT 250 260 270 280 290 300 ERSAPHCKKL SLELGGKNPA IIFADADMEQ CISTTVRSSF SNQGEICLCT SRIFVERSVY 310 320 330 340 350 360 PEFLTRFVEA TRRWKTGVPS DPSNDNGALI SKEHLQKVKG YITLALAEGA QVHCGEGVDK 370 380 390 400 410 420 LALPQQNIGG YFMLPTIISG VKDSSALMQE EIFGPVTCVT PFDEEEEVIS RANNVRYGLS 430 440 450 460 470 480 ATVWSRDVGR VHRVARKLQA GLVWTNCWLV RDLNLPFGGM KHSGIGREGG KDSYHFFTEV KSVTVKH

References

[1]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (AUG-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Gut.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC081581 mRNA. Translation: AAH81581.1. BC152164 mRNA. Translation: AAI52165.1.
IPI	IPI00489851.
RefSeq	NP_001004540.1.
UniGene	Dr.16380
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	Q66I21.
Genome annotation databases
Ensembl	ENSDARG00000036776. Danio rerio. [Contig view]
GeneID	447801.
KEGG	dre:447801.
Organism-specific databases
ZFIN	ZDB-GENE-040912-3. aldh8a1.
Phylogenomic databases
HOGENOM	Q66I21.
HOVERGEN	Q66I21.
OMA	Q66I21. KNPAIIF.
Gene expression databases
ArrayExpress	Q66I21.
Bgee	Q66I21.
Family and domain databases
InterPro	IPR016160. Ald_DH_CS. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH. [Graphical view]
Gene3D	G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AL8A1_DANRE

Accession

Primary (citable) accession number: Q66I21

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 11, 2004
Last modified:	June 16, 2009
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Zebrafish annotation project