Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Microtubule-associated protein RP/EB family member 1

Gene

Mapre1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds to the plus end of microtubules and regulates the dynamics of the microtubule cytoskeleton. Promotes cytoplasmic microtubule nucleation and elongation. May be involved in spindle function by stabilizing microtubules and anchoring them at centrosomes. May play a role in cell migration (By similarity).By similarity

GO - Molecular functioni

  • microtubule plus-end binding Source: UniProtKB
  • poly(A) RNA binding Source: Ensembl

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

ReactomeiR-RNO-2467813. Separation of Sister Chromatids.
R-RNO-2500257. Resolution of Sister Chromatid Cohesion.
R-RNO-2565942. Regulation of PLK1 Activity at G2/M Transition.
R-RNO-380259. Loss of Nlp from mitotic centrosomes.
R-RNO-380270. Recruitment of mitotic centrosome proteins and complexes.
R-RNO-5620912. Anchoring of the basal body to the plasma membrane.
R-RNO-5663220. RHO GTPases Activate Formins.
R-RNO-68877. Mitotic Prometaphase.
R-RNO-8852276. The role of GTSE1 in G2/M progression after G2 checkpoint.
R-RNO-8854518. AURKA Activation by TPX2.

Names & Taxonomyi

Protein namesi
Recommended name:
Microtubule-associated protein RP/EB family member 1
Alternative name(s):
APC-binding protein EB1
End-binding protein 1
Short name:
EB1
Gene namesi
Name:Mapre1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi621781. Mapre1.

Subcellular locationi

  • Cytoplasm By similarity
  • Cytoplasmcytoskeleton By similarity
  • Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity

  • Note: Associated with the microtubule network at the growing distal tip of microtubules. Detected along microtubules. Also enriched at the centrosome (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 268267Microtubule-associated protein RP/EB family member 1PRO_0000213419Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine1 Publication
Modified residuei124 – 1241PhosphotyrosineBy similarity
Modified residuei155 – 1551PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ66HR2.
PRIDEiQ66HR2.

PTM databases

iPTMnetiQ66HR2.

Expressioni

Gene expression databases

ExpressionAtlasiQ66HR2. baseline and differential.
GenevisibleiQ66HR2. RN.

Interactioni

Subunit structurei

Homodimer. Heterodimer with MAPRE3. Interacts with DCTN1, DCTN2, DIAPH1, DIAPH2, TERF1 and dynein intermediate chain. Interacts with APC (via C-terminal domain), CLASP2, DST, KIF2C and STIM1; probably required for their targeting to the growing microtubule plus ends. Interacts with MTUS2; interaction is direct and probably targets MTUS2 to microtubules. Interacts with APC2. Interacts with CLASP1 (By similarity). According to PubMed:19553473, MAPRE1 does not interact with CDK5RAP2 (By similarity). Interacts with MACF1. Interacts (via C-terminus) with CLIP1. Interacts with SLAIN (By similarity). Interacts with KIF18B; this interaction is required for efficient accumulation of KIF18B at microtubule plus ends (By similarity). Interacts with MISP (By similarity). Interacts with KCNAB2 (PubMed:21357749) Interacts with RABL2/RABL2A; binds preferentially to GTP-bound RABL2. Interacts with KNSTRN. Interacts with NCKAP5L (By similarity).By similarity1 Publication

GO - Molecular functioni

  • microtubule plus-end binding Source: UniProtKB

Protein-protein interaction databases

BioGridi250409. 1 interaction.
IntActiQ66HR2. 1 interaction.
STRINGi10116.ENSRNOP00000016220.

Structurei

3D structure databases

ProteinModelPortaliQ66HR2.
SMRiQ66HR2. Positions 1-130, 191-257.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini14 – 116103CHPROSITE-ProRule annotationAdd
BLAST
Domaini185 – 25571EB1 C-terminalPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni124 – 268145Interaction with MTUS2/TIP150By similarityAdd
BLAST
Regioni208 – 26861DCTN1-bindingBy similarityAdd
BLAST
Regioni220 – 24223APC-bindingBy similarityAdd
BLAST

Domaini

Composed of two functionally independent domains. The N-terminal domain forms a hydrophobic cleft involved in microtubule binding and the C-terminal is involved in the formation of mutually exclusive complexes with APC and DCTN1 (By similarity).By similarity

Sequence similaritiesi

Belongs to the MAPRE family.Curated
Contains 1 CH (calponin-homology) domain.PROSITE-ProRule annotation
Contains 1 EB1 C-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3000. Eukaryota.
COG5217. LUCA.
GeneTreeiENSGT00490000043329.
HOGENOMiHOG000198048.
HOVERGENiHBG052410.
InParanoidiQ66HR2.
KOiK10436.
OMAiPMSTQRT.
OrthoDBiEOG7ZD1W5.
PhylomeDBiQ66HR2.
TreeFamiTF313620.

Family and domain databases

Gene3Di1.10.418.10. 1 hit.
InterProiIPR001715. CH-domain.
IPR004953. EB1_C.
IPR027328. MAPRE.
[Graphical view]
PANTHERiPTHR10623. PTHR10623. 1 hit.
PfamiPF00307. CH. 1 hit.
PF03271. EB1. 1 hit.
[Graphical view]
SUPFAMiSSF140612. SSF140612. 1 hit.
SSF47576. SSF47576. 1 hit.
PROSITEiPS50021. CH. 1 hit.
PS51230. EB1_C. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q66HR2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVNVYSTSV TSDNLSRHDM LAWINESLQL NLTKIEQLCS GAAYCQFMDM
60 70 80 90 100
LFPGSIALKK VKFQAKLEHE YIQNFKILQA GFKRMGVDKI IPVDKLVKGK
110 120 130 140 150
FQDNFEFVQW FKKFFDANYD GKEYDPVAAR QGQETAVAPS LVAPALSKPK
160 170 180 190 200
KPLGSGSAAP QRPIATQRTT AAPKAGPGMV RKNPGMGNGD DEAAELMQQV
210 220 230 240 250
KVLKLTVEDL EKERDFYFGK LRNIELICQE NEGENDPVLQ RIVDILYATD
260
EGFVIPDEGG PQEEQEEY
Length:268
Mass (Da):30,004
Last modified:January 23, 2007 - v3
Checksum:iCEDE13116FC79F93
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC081726 mRNA. Translation: AAH81726.1.
RefSeqiNP_612518.2. NM_138509.3.
XP_006235356.1. XM_006235294.2.
XP_006235357.1. XM_006235295.2.
UniGeneiRn.7652.

Genome annotation databases

EnsembliENSRNOT00000016220; ENSRNOP00000016220; ENSRNOG00000011798.
GeneIDi114764.
KEGGirno:114764.
UCSCiRGD:621781. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC081726 mRNA. Translation: AAH81726.1.
RefSeqiNP_612518.2. NM_138509.3.
XP_006235356.1. XM_006235294.2.
XP_006235357.1. XM_006235295.2.
UniGeneiRn.7652.

3D structure databases

ProteinModelPortaliQ66HR2.
SMRiQ66HR2. Positions 1-130, 191-257.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi250409. 1 interaction.
IntActiQ66HR2. 1 interaction.
STRINGi10116.ENSRNOP00000016220.

PTM databases

iPTMnetiQ66HR2.

Proteomic databases

PaxDbiQ66HR2.
PRIDEiQ66HR2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000016220; ENSRNOP00000016220; ENSRNOG00000011798.
GeneIDi114764.
KEGGirno:114764.
UCSCiRGD:621781. rat.

Organism-specific databases

CTDi22919.
RGDi621781. Mapre1.

Phylogenomic databases

eggNOGiKOG3000. Eukaryota.
COG5217. LUCA.
GeneTreeiENSGT00490000043329.
HOGENOMiHOG000198048.
HOVERGENiHBG052410.
InParanoidiQ66HR2.
KOiK10436.
OMAiPMSTQRT.
OrthoDBiEOG7ZD1W5.
PhylomeDBiQ66HR2.
TreeFamiTF313620.

Enzyme and pathway databases

ReactomeiR-RNO-2467813. Separation of Sister Chromatids.
R-RNO-2500257. Resolution of Sister Chromatid Cohesion.
R-RNO-2565942. Regulation of PLK1 Activity at G2/M Transition.
R-RNO-380259. Loss of Nlp from mitotic centrosomes.
R-RNO-380270. Recruitment of mitotic centrosome proteins and complexes.
R-RNO-5620912. Anchoring of the basal body to the plasma membrane.
R-RNO-5663220. RHO GTPases Activate Formins.
R-RNO-68877. Mitotic Prometaphase.
R-RNO-8852276. The role of GTSE1 in G2/M progression after G2 checkpoint.
R-RNO-8854518. AURKA Activation by TPX2.

Miscellaneous databases

PROiQ66HR2.

Gene expression databases

ExpressionAtlasiQ66HR2. baseline and differential.
GenevisibleiQ66HR2. RN.

Family and domain databases

Gene3Di1.10.418.10. 1 hit.
InterProiIPR001715. CH-domain.
IPR004953. EB1_C.
IPR027328. MAPRE.
[Graphical view]
PANTHERiPTHR10623. PTHR10623. 1 hit.
PfamiPF00307. CH. 1 hit.
PF03271. EB1. 1 hit.
[Graphical view]
SUPFAMiSSF140612. SSF140612. 1 hit.
SSF47576. SSF47576. 1 hit.
PROSITEiPS50021. CH. 1 hit.
PS51230. EB1_C. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Heart.
  2. Bienvenut W.V., von Kriegsheim A.F., Kolch W.
    Submitted (AUG-2006) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 2-17; 67-98; 113-150; 182-201 AND 205-214, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Pheochromocytoma.
  3. "Interaction of CDK5RAP2 with EB1 to track growing microtubule tips and to regulate microtubule dynamics."
    Fong K.W., Hau S.Y., Kho Y.S., Jia Y., He L., Qi R.Z.
    Mol. Biol. Cell 20:3660-3670(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT.
  4. "Cdk-mediated phosphorylation of the Kvbeta2 auxiliary subunit regulates Kv1 channel axonal targeting."
    Vacher H., Yang J.W., Cerda O., Autillo-Touati A., Dargent B., Trimmer J.S.
    J. Cell Biol. 192:813-824(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH KCNAB2.

Entry informationi

Entry nameiMARE1_RAT
AccessioniPrimary (citable) accession number: Q66HR2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: January 23, 2007
Last modified: July 6, 2016
This is version 110 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.