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Protein

Microtubule-associated protein RP/EB family member 1

Gene

Mapre1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plus-end tracking protein (+TIP) that binds to the plus-end of microtubules and regulates the dynamics of the microtubule cytoskeleton. Promotes cytoplasmic microtubule nucleation and elongation. May be involved in spindle function by stabilizing microtubules and anchoring them at centrosomes. Also acts as a regulator of minus-end microtubule organization: interacts with the complex formed by AKAP9 and PDE4DIP, leading to recruit CAMSAP2 to the Golgi apparatus, thereby tethering non-centrosomal minus-end microtubules to the Golgi, an important step for polarized cell movement. Promotes elongation of CAMSAP2-decorated microtubule stretches on the minus-end of microtubules. Acts as a regulator of autophagosome transport via interaction with CAMSAP2 (By similarity). May play a role in cell migration (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processCell cycle, Cell division, Mitosis

Enzyme and pathway databases

ReactomeiR-RNO-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal
R-RNO-2467813 Separation of Sister Chromatids
R-RNO-2500257 Resolution of Sister Chromatid Cohesion
R-RNO-2565942 Regulation of PLK1 Activity at G2/M Transition
R-RNO-380259 Loss of Nlp from mitotic centrosomes
R-RNO-380270 Recruitment of mitotic centrosome proteins and complexes
R-RNO-380320 Recruitment of NuMA to mitotic centrosomes
R-RNO-5620912 Anchoring of the basal body to the plasma membrane
R-RNO-5663220 RHO GTPases Activate Formins
R-RNO-68877 Mitotic Prometaphase
R-RNO-8852276 The role of GTSE1 in G2/M progression after G2 checkpoint
R-RNO-8854518 AURKA Activation by TPX2

Names & Taxonomyi

Protein namesi
Recommended name:
Microtubule-associated protein RP/EB family member 1
Alternative name(s):
APC-binding protein EB1
End-binding protein 1
Short name:
EB1
Gene namesi
Name:Mapre1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi621781 Mapre1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00002134192 – 268Microtubule-associated protein RP/EB family member 1Add BLAST267

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanine1 Publication1
Modified residuei124PhosphotyrosineBy similarity1
Modified residuei155PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ66HR2
PRIDEiQ66HR2

PTM databases

iPTMnetiQ66HR2
PhosphoSitePlusiQ66HR2

Expressioni

Gene expression databases

BgeeiENSRNOG00000011798
ExpressionAtlasiQ66HR2 baseline and differential
GenevisibleiQ66HR2 RN

Interactioni

Subunit structurei

Homodimer. Heterodimer with MAPRE3. Interacts with DCTN1, DCTN2, TERF1 and dynein intermediate chain (By similarity). Interaction with DIAPH1 and DIAPH2 (By similarity). Interacts with APC (via C-terminal domain), CLASP2, DST, KIF2C and STIM1; probably required for their targeting to the growing microtubule plus ends. Interacts with MTUS2; interaction is direct and probably targets MTUS2 to microtubules. Interacts with APC2. Interacts with CLASP1. Interacts with CDK5RAP2 (By similarity). According to another report, MAPRE1 does not interact with CDK5RAP2 (PubMed:19553473). Interacts with MACF1 (By similarity). Interacts with RABL2/RABL2A; binds preferentially to GTP-bound RABL2 (By similarity). Interacts with KCNAB2 (PubMed:21357749). Interacts (via C-terminus) with CLIP1. Interacts with SLAIN2 and SLAIN1. Interacts with KIF18B; this interaction is required for efficient accumulation of KIF18B at microtubule plus ends. Interacts with MISP. Interacts with KNSTRN. Interacts with NCKAP5L. Interacts with AKAP9. Interacts with PDE4DIP; this interaction, which is PDE4DIP isoform-specific, is required for its recruitment to the Golgi apparatus. Interacts with CAMSAP2 (By similarity). May form a pericentrosomal complex with AKAP9, CDK5RAP2 and PDE4DIP isoform 2/MMG8/SMYLE; within this complex, MAPRE1 binding to CDK5RAP2 may be mediated by PDE4DIP (By similarity). Contrary to other mammalian species, does not interact with CDK5RAP2, possibly due to the lack of conservation of the MAPRE1-binding motif in rat CDK5RAP2 (Probable).By similarity1 Publication2 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi2504091 interactor.
IntActiQ66HR2 1 interactor.
MINTiQ66HR2
STRINGi10116.ENSRNOP00000016220

Structurei

3D structure databases

ProteinModelPortaliQ66HR2
SMRiQ66HR2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini14 – 116Calponin-homology (CH)PROSITE-ProRule annotationAdd BLAST103
Domaini185 – 255EB1 C-terminalPROSITE-ProRule annotationAdd BLAST71

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni124 – 268Interaction with MTUS2/TIP150By similarityAdd BLAST145
Regioni208 – 268DCTN1-bindingBy similarityAdd BLAST61
Regioni220 – 242APC-bindingBy similarityAdd BLAST23

Domaini

Composed of two functionally independent domains. The N-terminal domain forms a hydrophobic cleft involved in microtubule binding and the C-terminal is involved in the formation of mutually exclusive complexes with APC and DCTN1.By similarity

Sequence similaritiesi

Belongs to the MAPRE family.Curated

Phylogenomic databases

eggNOGiKOG3000 Eukaryota
COG5217 LUCA
GeneTreeiENSGT00490000043329
HOGENOMiHOG000198048
HOVERGENiHBG052410
InParanoidiQ66HR2
KOiK10436
OMAiCQVFDSI
OrthoDBiEOG091G0FT4
PhylomeDBiQ66HR2
TreeFamiTF313620

Family and domain databases

Gene3Di1.10.418.101 hit
InterProiView protein in InterPro
IPR001715 CH-domain
IPR036872 CH_dom_sf
IPR004953 EB1_C
IPR036133 EB1_C_sf
IPR027739 EB1_Meta
IPR027328 MAPRE
PANTHERiPTHR10623 PTHR10623, 1 hit
PTHR10623:SF20 PTHR10623:SF20, 1 hit
PfamiView protein in Pfam
PF00307 CH, 1 hit
PF03271 EB1, 1 hit
SUPFAMiSSF140612 SSF140612, 1 hit
SSF47576 SSF47576, 1 hit
PROSITEiView protein in PROSITE
PS50021 CH, 1 hit
PS51230 EB1_C, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q66HR2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVNVYSTSV TSDNLSRHDM LAWINESLQL NLTKIEQLCS GAAYCQFMDM
60 70 80 90 100
LFPGSIALKK VKFQAKLEHE YIQNFKILQA GFKRMGVDKI IPVDKLVKGK
110 120 130 140 150
FQDNFEFVQW FKKFFDANYD GKEYDPVAAR QGQETAVAPS LVAPALSKPK
160 170 180 190 200
KPLGSGSAAP QRPIATQRTT AAPKAGPGMV RKNPGMGNGD DEAAELMQQV
210 220 230 240 250
KVLKLTVEDL EKERDFYFGK LRNIELICQE NEGENDPVLQ RIVDILYATD
260
EGFVIPDEGG PQEEQEEY
Length:268
Mass (Da):30,004
Last modified:January 23, 2007 - v3
Checksum:iCEDE13116FC79F93
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC081726 mRNA Translation: AAH81726.1
RefSeqiNP_612518.2, NM_138509.3
XP_006235356.1, XM_006235294.3
XP_006235357.1, XM_006235295.3
UniGeneiRn.7652

Genome annotation databases

EnsembliENSRNOT00000016220; ENSRNOP00000016220; ENSRNOG00000011798
GeneIDi114764
KEGGirno:114764
UCSCiRGD:621781 rat

Similar proteinsi

Entry informationi

Entry nameiMARE1_RAT
AccessioniPrimary (citable) accession number: Q66HR2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 128 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome