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Protein

AP-2 complex subunit alpha

Gene

Ap2a2

Organism
Rattus norvegicus (Rat)
Status
Unreviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha subunit binds polyphosphoinositide-containing lipids, positioning AP-2 on the membrane. The AP-2 alpha subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei43Phosphatidylinositol lipid headgroupUniRule annotation1
Binding sitei53Phosphatidylinositol lipid headgroupUniRule annotation1
Binding sitei57Phosphatidylinositol lipid headgroupUniRule annotation1
Binding sitei58Phosphatidylinositol lipid headgroupUniRule annotation1
Binding sitei61Phosphatidylinositol lipid headgroupUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processEndocytosisUniRule annotation, Protein transportUniRule annotationSAAS annotation, Transport

Enzyme and pathway databases

ReactomeiR-RNO-177504 Retrograde neurotrophin signalling
R-RNO-2132295 MHC class II antigen presentation
R-RNO-416993 Trafficking of GluR2-containing AMPA receptors
R-RNO-437239 Recycling pathway of L1
R-RNO-5099900 WNT5A-dependent internalization of FZD4
R-RNO-5140745 WNT5A-dependent internalization of FZD2, FZD5 and ROR2
R-RNO-6798695 Neutrophil degranulation
R-RNO-8856825 Cargo recognition for clathrin-mediated endocytosis
R-RNO-8856828 Clathrin-mediated endocytosis
R-RNO-8866427 VLDLR internalisation and degradation
R-RNO-8964038 LDL clearance

Names & Taxonomyi

Protein namesi
Recommended name:
AP-2 complex subunit alphaUniRule annotation
Gene namesi
Name:Ap2a2Imported
OrganismiRattus norvegicus (Rat)Imported
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi

Organism-specific databases

RGDi71015 Ap2a2

Subcellular locationi

Keywords - Cellular componenti

Coated pitUniRule annotation, Membrane

PTM / Processingi

Proteomic databases

PRIDEiQ66HM2

Expressioni

Gene expression databases

BgeeiENSRNOG00000019534
ExpressionAtlasiQ66HM2 baseline and differential

Interactioni

Subunit structurei

Adaptor protein complex 2 (AP-2) is a heterotetramer composed of two large adaptins (alpha-type subunit AP2A1 or AP2A2 and beta-type subunit AP2B1), a medium adaptin (mu-type subunit AP2M1) and a small adaptin (sigma-type subunit AP2S1).UniRule annotation

Binary interactionsi

WithEntry#Exp.IntActNotes
NumbQ3MUI12EBI-2255846,EBI-7007865

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ66HM2, 2 interactors

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2XA7X-ray3.10A1-621[»]
SMRiQ66HM2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini707 – 820Alpha_adaptinC2InterPro annotationAdd BLAST114

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili23 – 43Sequence analysisAdd BLAST21

Sequence similaritiesi

Belongs to the adaptor complexes large subunit family.UniRule annotation

Keywords - Domaini

Coiled coilSequence analysis

Phylogenomic databases

eggNOGiKOG1077 Eukaryota
ENOG410XNQE LUCA
GeneTreeiENSGT00550000074757
HOGENOMiHOG000170596
HOVERGENiHBG050518
KOiK11824
OMAiFMNLALH
OrthoDBiEOG091G01JZ
TreeFamiTF300308

Family and domain databases

Gene3Di1.25.10.10, 1 hit
3.30.310.10, 1 hit
InterProiView protein in InterPro
IPR017104 AP2_complex_asu
IPR011989 ARM-like
IPR016024 ARM-type_fold
IPR002553 Clathrin/coatomer_adapt-like_N
IPR003164 Clathrin_a-adaptin_app_sub_C
IPR008152 Clathrin_a/b/g-adaptin_app_Ig
IPR013041 Clathrin_app_Ig-like_sf
IPR009028 Coatomer/calthrin_app_sub_C
IPR012295 TBP_dom_sf
PfamiView protein in Pfam
PF01602 Adaptin_N, 1 hit
PF02296 Alpha_adaptin_C, 1 hit
PF02883 Alpha_adaptinC2, 1 hit
PIRSFiPIRSF037091 AP2_complex_alpha, 1 hit
SMARTiView protein in SMART
SM00809 Alpha_adaptinC2, 1 hit
SUPFAMiSSF48371 SSF48371, 1 hit
SSF49348 SSF49348, 1 hit
SSF55711 SSF55711, 1 hit

Sequencei

Sequence statusi: Complete.

Q66HM2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPAVSKGEGM RGLAVFISDI RNCKSKEAEI KRINKELANI RSKFKGDKAL
60 70 80 90 100
DGYSKKKYVC KLLFIFLLGH DIDFGHMEAV NLLSSNRYTE KQIGYLFISV
110 120 130 140 150
LVNSNSELIR LINNAIKNDL ASRNPTFMGL ALHCIANVGS REMAEAFAGE
160 170 180 190 200
IPKILVAGDT MDSVKQSAAL CLLRLYRTSP DLVPMGDWTS RVVHLLNDQH
210 220 230 240 250
LGVVTAATSL ITTLAQKNPE EFKTSVSLAV SRLSRIVTSA STDLQDYTYY
260 270 280 290 300
FVPAPWLSVK LLRLLQCYPP PEDPAVRGRL TECLETILNK AQEPPKSKKV
310 320 330 340 350
QHSNAKNAVL FEAISLIIHH DSEPNLLVRA CNQLGQFLQH RETNLRYLAL
360 370 380 390 400
ESMCTLASSE FSHEAVKTHI ETVINALKTE RDVSVRQRAV DLLYAMCDRS
410 420 430 440 450
NAQQIVAEML SYLETADYSI REEIVLKVAI LAEKYAVDYT WYVDTILNLI
460 470 480 490 500
RIAGDYVSEE VWYRVIQIVI NRDDVQGYAA KTVFEALQAP ACHENLVKVG
510 520 530 540 550
GYILGEFGNL IAGDPRSSPL IQFNLLHSKF HLCSVPTRAL LLSTYIKFVN
560 570 580 590 600
LFPEVKATIQ DVLRSDSQLK NADVELQQRA VEYLRLSTVA STDILATVLE
610 620 630 640 650
EMPPFPERES SILAKLKKKK GPSTVTDLEE TKRERSIDVN GGPEPVPAST
660 670 680 690 700
SAASTPSPSA DLLGLGAVPP APTGPPPTSG GGLLVDVFSD SASAVAPLAP
710 720 730 740 750
GSEDNFARFV CKNNGVLFEN QLLQIGLKSE FRQNLGRMFI FYGNKTSTQF
760 770 780 790 800
LNFTPTLICA DDLQTNLNLQ TKPVDPTVDG GAQVQQVVNI ECISDFTEAP
810 820 830 840 850
VLNIQFRYGG TFQNVSVKLP ITLNKFFQPT EMASQDFFQR WKQLSNPQQE
860 870 880 890 900
VQNIFKAKHP MDTEITKAKI IGFGSALLEE VDPNPANFVG AGIIHTKTTQ
910 920 930
IGCLLRLEPN LQAQMYRLTL RTSKDTVSQR LCELLSEQF
Length:939
Mass (Da):104,174
Last modified:October 11, 2004 - v1
Checksum:iD6A7EB7EE919F566
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC109542 Genomic DNA No translation available.
BC081786 mRNA Translation: AAH81786.1
RefSeqiNP_112270.2, NM_031008.2
UniGeneiRn.34928

Genome annotation databases

EnsembliENSRNOT00000064254; ENSRNOP00000060992; ENSRNOG00000019534
GeneIDi81637
KEGGirno:81637

Entry informationi

Entry nameiQ66HM2_RAT
AccessioniPrimary (citable) accession number: Q66HM2
Entry historyiIntegrated into UniProtKB/TrEMBL: October 11, 2004
Last sequence update: October 11, 2004
Last modified: May 23, 2018
This is version 128 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources, Complete proteome, Proteomics identificationCombined sources, Reference proteomeImported

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