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Reviewed, UniProtKB/Swiss-Prot Q66HH1 (B4GT4_RAT)

Last modified November 3, 2009. Version 47. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Beta-1,4-galactosyltransferase 4
      Short name=Beta-1,4-GalTase 4
      Short name=Beta4Gal-T4
      Short name=b4Gal-T4
    EC=2.4.1.-
Alternative name(s):
    UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 4
    UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 4
Including the following 2 domains:
    1- Recommended name:
            N-acetyllactosamine synthase
              EC=2.4.1.90
        Alternative name(s):
            Nal synthetase
    2- Recommended name:
            Beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase
              EC=2.4.1.-
Gene names
Name: B4galt4
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length344 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at transcript level.

General annotation (Comments)

Function

Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids By similarity.

Catalytic activity

UDP-galactose + N-acetyl-D-glucosamine = UDP + N-acetyllactosamine.

Cofactor

Manganese By similarity.

Pathway

Protein modification; protein glycosylation.

Subcellular location

Golgi apparatusGolgi stack membrane; Single-pass type II membrane protein By similarity. Note: Trans cisternae of Golgi stack By similarity.

Sequence similarities

Belongs to the glycosyltransferase 7 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 344344Beta-1,4-galactosyltransferase 4
PRO_0000080544

Regions

Topological domain1 – 1212Cytoplasmic Potential
Transmembrane13 – 3826Signal-anchor for type II membrane protein Potential
Topological domain39 – 344306Lumenal Potential

Sites

Metal binding1961Manganese By similarity
Metal binding2891Manganese By similarity

Amino acid modifications

Glycosylation2201N-linked (GlcNAc...) Potential
Glycosylation3351N-linked (GlcNAc...) Potential
Disulfide bond77 ↔ 118 By similarity
Disulfide bond189 ↔ 208 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q66HH1-1 [UniParc].

Last modified October 11, 2004. Version 1.
Checksum: 864967A3E87A3CC5

FASTA34439,707
        10         20         30         40         50         60 
MGCNPPYLLS YRLRLLLLFT LCLTVLGWAT SNYFVGAIQV IPRAKNFMAT LHKVMYLGNE 

        70         80         90        100        110        120 
ETLGHGAAMK KAELANCPSV SPNLRGQNKL VFKPDLTLEE VQAKNPKVSR GRYRPEECKA 

       130        140        150        160        170        180 
LQRVAVLIPH RNREKHLIYL LEHLHPFLQR QQLDYGIYVI HQTGSKKFNR AKLLNVGYLE 

       190        200        210        220        230        240 
ALKEENWDCF IFHDVDLVPE NDFNLYTCGD QPKHLVVGRN STGYRLRYSK YFGGVTALSR 

       250        260        270        280        290        300 
EQFFKVNGFS NNYWGWGGED DDLRLRVELH KMKISRPKPD VGKYTMIFHT RDKGNEVNGS 

       310        320        330        340 
RMKLLQQMSR VWKTDGLSSC SYRLLSVEHN PLYANITVDF WTAA 

« Hide

References

[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Lung.
+Additional computationally mapped references.

Cross-references

Sequence databases

BC081866 mRNA. Translation: AAH81866.1.
IPIIPI00471883.
RefSeqNP_001012018.1.
UniGeneRn.143976

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRINGQ66HH1.

Protein family/group databases

CAZyGT7. Glycosyltransferase Family 7.

Genome annotation databases

EnsemblENSRNOT00000004289; ENSRNOP00000004289; ENSRNOG00000003114; Rattus norvegicus. [Genome view]
GeneID303923.
KEGGrno:303923.
UCSCNM_001012018. rat.

Organism-specific databases

CTD303923.
RGD1307880. B4galt4.

Phylogenomic databases

HOVERGENQ66HH1.
OMAVWRTDGL.

Enzyme and pathway databases

BRENDA2.4.1.90. 248.

Gene expression databases

ArrayExpressQ66HH1.
GenevestigatorQ66HH1.
GermOnlineENSRNOG00000003114. Rattus norvegicus.

Family and domain databases

InterProIPR003859. Galactosyl_T_2.
[Graphical view]
PANTHERPTHR19300. Galactosyl_T_2. 1 hit.
PfamPF02709. Galactosyl_T_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio652347.

Entry information

Entry nameB4GT4_RAT
AccessionPrimary (citable) accession number: Q66HH1
Entry history
Integrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: October 11, 2004
Last modified: November 3, 2009
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents