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Q66HH1

- B4GT4_RAT

UniProt

Q66HH1 - B4GT4_RAT

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Protein
Beta-1,4-galactosyltransferase 4
Gene
B4galt4
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids By similarity.

Catalytic activityi

UDP-alpha-D-galactose + N-acetyl-D-glucosamine = UDP + N-acetyllactosamine.
UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide = UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide.

Cofactori

Manganese By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi196 – 1961Manganese By similarity
Binding sitei224 – 2241UDP-alpha-D-galactose By similarity
Binding sitei256 – 2561UDP-alpha-D-galactose By similarity
Metal bindingi289 – 2891Manganese; via tele nitrogen By similarity
Binding sitei301 – 3011N-acetyl-D-glucosamine By similarity

GO - Molecular functioni

  1. N-acetyllactosamine synthase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein glycosylation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_196475. Keratan sulfate biosynthesis.
REACT_199227. N-Glycan antennae elongation.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT7. Glycosyltransferase Family 7.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-1,4-galactosyltransferase 4 (EC:2.4.1.-)
Short name:
Beta-1,4-GalTase 4
Short name:
Beta4Gal-T4
Short name:
b4Gal-T4
Alternative name(s):
UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 4
UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 4
Including the following 2 domains:
N-acetyllactosamine synthase (EC:2.4.1.90)
Alternative name(s):
Nal synthase
Lactotriaosylceramide beta-1,4-galactosyltransferase (EC:2.4.1.275)
Alternative name(s):
Beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase
Gene namesi
Name:B4galt4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 11

Organism-specific databases

RGDi1307880. B4galt4.

Subcellular locationi

Golgi apparatusGolgi stack membrane; Single-pass type II membrane protein By similarity
Note: Trans cisternae of Golgi stack By similarity.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1212Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei13 – 3826Helical; Signal-anchor for type II membrane protein; Reviewed prediction
Add
BLAST
Topological domaini39 – 344306Lumenal Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. Golgi cisterna membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 344344Beta-1,4-galactosyltransferase 4
PRO_0000080544Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi77 ↔ 118 By similarity
Disulfide bondi189 ↔ 208 By similarity
Glycosylationi220 – 2201N-linked (GlcNAc...) Reviewed prediction
Glycosylationi335 – 3351N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Gene expression databases

GenevestigatoriQ66HH1.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000004289.

Structurei

3D structure databases

ProteinModelPortaliQ66HH1.
SMRiQ66HH1. Positions 74-344.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni129 – 1335UDP-alpha-D-galactose binding By similarity
Regioni168 – 1703UDP-alpha-D-galactose binding By similarity
Regioni195 – 1962UDP-alpha-D-galactose binding By similarity
Regioni258 – 2614N-acetyl-D-glucosamine binding By similarity
Regioni289 – 2913UDP-alpha-D-galactose binding By similarity

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG327897.
GeneTreeiENSGT00740000115031.
HOGENOMiHOG000231027.
HOVERGENiHBG058334.
InParanoidiQ66HH1.
KOiK07969.
OMAiHTRDKGN.
OrthoDBiEOG7060R0.
PhylomeDBiQ66HH1.
TreeFamiTF312834.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR003859. Galactosyl_T.
IPR027791. Galactosyl_T_C.
IPR027995. Galactosyl_T_N.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR19300. PTHR19300. 1 hit.
PfamiPF02709. Glyco_transf_7C. 1 hit.
PF13733. Glyco_transf_7N. 1 hit.
[Graphical view]
PRINTSiPR02050. B14GALTRFASE.
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

Q66HH1-1 [UniParc]FASTAAdd to Basket

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MGCNPPYLLS YRLRLLLLFT LCLTVLGWAT SNYFVGAIQV IPRAKNFMAT    50
LHKVMYLGNE ETLGHGAAMK KAELANCPSV SPNLRGQNKL VFKPDLTLEE 100
VQAKNPKVSR GRYRPEECKA LQRVAVLIPH RNREKHLIYL LEHLHPFLQR 150
QQLDYGIYVI HQTGSKKFNR AKLLNVGYLE ALKEENWDCF IFHDVDLVPE 200
NDFNLYTCGD QPKHLVVGRN STGYRLRYSK YFGGVTALSR EQFFKVNGFS 250
NNYWGWGGED DDLRLRVELH KMKISRPKPD VGKYTMIFHT RDKGNEVNGS 300
RMKLLQQMSR VWKTDGLSSC SYRLLSVEHN PLYANITVDF WTAA 344
Length:344
Mass (Da):39,707
Last modified:October 11, 2004 - v1
Checksum:i864967A3E87A3CC5
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC081866 mRNA. Translation: AAH81866.1.
RefSeqiNP_001012018.1. NM_001012018.1.
XP_006248428.1. XM_006248366.1.
UniGeneiRn.143976.

Genome annotation databases

EnsembliENSRNOT00000004289; ENSRNOP00000004289; ENSRNOG00000003114.
GeneIDi303923.
KEGGirno:303923.
UCSCiRGD:1307880. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC081866 mRNA. Translation: AAH81866.1 .
RefSeqi NP_001012018.1. NM_001012018.1.
XP_006248428.1. XM_006248366.1.
UniGenei Rn.143976.

3D structure databases

ProteinModelPortali Q66HH1.
SMRi Q66HH1. Positions 74-344.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000004289.

Protein family/group databases

CAZyi GT7. Glycosyltransferase Family 7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000004289 ; ENSRNOP00000004289 ; ENSRNOG00000003114 .
GeneIDi 303923.
KEGGi rno:303923.
UCSCi RGD:1307880. rat.

Organism-specific databases

CTDi 8702.
RGDi 1307880. B4galt4.

Phylogenomic databases

eggNOGi NOG327897.
GeneTreei ENSGT00740000115031.
HOGENOMi HOG000231027.
HOVERGENi HBG058334.
InParanoidi Q66HH1.
KOi K07969.
OMAi HTRDKGN.
OrthoDBi EOG7060R0.
PhylomeDBi Q66HH1.
TreeFami TF312834.

Enzyme and pathway databases

UniPathwayi UPA00378 .
Reactomei REACT_196475. Keratan sulfate biosynthesis.
REACT_199227. N-Glycan antennae elongation.

Miscellaneous databases

NextBioi 652347.
PROi Q66HH1.

Gene expression databases

Genevestigatori Q66HH1.

Family and domain databases

Gene3Di 3.90.550.10. 1 hit.
InterProi IPR003859. Galactosyl_T.
IPR027791. Galactosyl_T_C.
IPR027995. Galactosyl_T_N.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view ]
PANTHERi PTHR19300. PTHR19300. 1 hit.
Pfami PF02709. Glyco_transf_7C. 1 hit.
PF13733. Glyco_transf_7N. 1 hit.
[Graphical view ]
PRINTSi PR02050. B14GALTRFASE.
SUPFAMi SSF53448. SSF53448. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lung.

Entry informationi

Entry nameiB4GT4_RAT
AccessioniPrimary (citable) accession number: Q66HH1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: October 11, 2004
Last modified: September 3, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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