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Protein

Beta-1,4-galactosyltransferase 4

Gene

B4galt4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.By similarity

Catalytic activityi

UDP-alpha-D-galactose + N-acetyl-D-glucosamine = UDP + N-acetyllactosamine.
UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide = UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide.

Cofactori

Mn2+By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi196ManganeseBy similarity1
Binding sitei224UDP-alpha-D-galactoseBy similarity1
Binding sitei256UDP-alpha-D-galactoseBy similarity1
Metal bindingi289Manganese; via tele nitrogenBy similarity1
Binding sitei301N-acetyl-D-glucosamineBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

ReactomeiR-RNO-2022854. Keratan sulfate biosynthesis.
R-RNO-975577. N-Glycan antennae elongation.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT7. Glycosyltransferase Family 7.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-1,4-galactosyltransferase 4 (EC:2.4.1.-)
Short name:
Beta-1,4-GalTase 4
Short name:
Beta4Gal-T4
Short name:
b4Gal-T4
Alternative name(s):
UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 4
UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 4
Including the following 2 domains:
N-acetyllactosamine synthase (EC:2.4.1.90)
Alternative name(s):
Nal synthase
Lactotriaosylceramide beta-1,4-galactosyltransferase (EC:2.4.1.275)
Alternative name(s):
Beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase
Gene namesi
Name:B4galt4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 11

Organism-specific databases

RGDi1307880. B4galt4.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 12CytoplasmicSequence analysisAdd BLAST12
Transmembranei13 – 38Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST26
Topological domaini39 – 344LumenalSequence analysisAdd BLAST306

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000805441 – 344Beta-1,4-galactosyltransferase 4Add BLAST344

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi77 ↔ 118By similarity
Disulfide bondi189 ↔ 208By similarity
Glycosylationi220N-linked (GlcNAc...)Sequence analysis1
Glycosylationi335N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ66HH1.

Expressioni

Gene expression databases

BgeeiENSRNOG00000003114.
GenevisibleiQ66HH1. RN.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000004289.

Structurei

3D structure databases

ProteinModelPortaliQ66HH1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni129 – 133UDP-alpha-D-galactose bindingBy similarity5
Regioni168 – 170UDP-alpha-D-galactose bindingBy similarity3
Regioni195 – 196UDP-alpha-D-galactose bindingBy similarity2
Regioni258 – 261N-acetyl-D-glucosamine bindingBy similarity4
Regioni289 – 291UDP-alpha-D-galactose bindingBy similarity3

Sequence similaritiesi

Belongs to the glycosyltransferase 7 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3916. Eukaryota.
ENOG410ZYYA. LUCA.
GeneTreeiENSGT00760000119140.
HOGENOMiHOG000231027.
HOVERGENiHBG058334.
InParanoidiQ66HH1.
KOiK07969.
OMAiHTRDKGN.
OrthoDBiEOG091G0P66.
PhylomeDBiQ66HH1.
TreeFamiTF312834.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR003859. Galactosyl_T.
IPR027791. Galactosyl_T_C.
IPR027995. Galactosyl_T_N.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR19300. PTHR19300. 1 hit.
PfamiPF02709. Glyco_transf_7C. 1 hit.
PF13733. Glyco_transf_7N. 1 hit.
[Graphical view]
PRINTSiPR02050. B14GALTRFASE.
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

Q66HH1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGCNPPYLLS YRLRLLLLFT LCLTVLGWAT SNYFVGAIQV IPRAKNFMAT
60 70 80 90 100
LHKVMYLGNE ETLGHGAAMK KAELANCPSV SPNLRGQNKL VFKPDLTLEE
110 120 130 140 150
VQAKNPKVSR GRYRPEECKA LQRVAVLIPH RNREKHLIYL LEHLHPFLQR
160 170 180 190 200
QQLDYGIYVI HQTGSKKFNR AKLLNVGYLE ALKEENWDCF IFHDVDLVPE
210 220 230 240 250
NDFNLYTCGD QPKHLVVGRN STGYRLRYSK YFGGVTALSR EQFFKVNGFS
260 270 280 290 300
NNYWGWGGED DDLRLRVELH KMKISRPKPD VGKYTMIFHT RDKGNEVNGS
310 320 330 340
RMKLLQQMSR VWKTDGLSSC SYRLLSVEHN PLYANITVDF WTAA
Length:344
Mass (Da):39,707
Last modified:October 11, 2004 - v1
Checksum:i864967A3E87A3CC5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC081866 mRNA. Translation: AAH81866.1.
RefSeqiNP_001012018.1. NM_001012018.1.
XP_017453489.1. XM_017598000.1.
UniGeneiRn.143976.

Genome annotation databases

EnsembliENSRNOT00000004289; ENSRNOP00000004289; ENSRNOG00000003114.
GeneIDi303923.
KEGGirno:303923.
UCSCiRGD:1307880. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC081866 mRNA. Translation: AAH81866.1.
RefSeqiNP_001012018.1. NM_001012018.1.
XP_017453489.1. XM_017598000.1.
UniGeneiRn.143976.

3D structure databases

ProteinModelPortaliQ66HH1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000004289.

Protein family/group databases

CAZyiGT7. Glycosyltransferase Family 7.

Proteomic databases

PaxDbiQ66HH1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000004289; ENSRNOP00000004289; ENSRNOG00000003114.
GeneIDi303923.
KEGGirno:303923.
UCSCiRGD:1307880. rat.

Organism-specific databases

CTDi8702.
RGDi1307880. B4galt4.

Phylogenomic databases

eggNOGiKOG3916. Eukaryota.
ENOG410ZYYA. LUCA.
GeneTreeiENSGT00760000119140.
HOGENOMiHOG000231027.
HOVERGENiHBG058334.
InParanoidiQ66HH1.
KOiK07969.
OMAiHTRDKGN.
OrthoDBiEOG091G0P66.
PhylomeDBiQ66HH1.
TreeFamiTF312834.

Enzyme and pathway databases

UniPathwayiUPA00378.
ReactomeiR-RNO-2022854. Keratan sulfate biosynthesis.
R-RNO-975577. N-Glycan antennae elongation.

Miscellaneous databases

PROiQ66HH1.

Gene expression databases

BgeeiENSRNOG00000003114.
GenevisibleiQ66HH1. RN.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR003859. Galactosyl_T.
IPR027791. Galactosyl_T_C.
IPR027995. Galactosyl_T_N.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR19300. PTHR19300. 1 hit.
PfamiPF02709. Glyco_transf_7C. 1 hit.
PF13733. Glyco_transf_7N. 1 hit.
[Graphical view]
PRINTSiPR02050. B14GALTRFASE.
SUPFAMiSSF53448. SSF53448. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiB4GT4_RAT
AccessioniPrimary (citable) accession number: Q66HH1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: October 11, 2004
Last modified: November 30, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.