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Q66HH1 (B4GT4_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-1,4-galactosyltransferase 4

Short name=Beta-1,4-GalTase 4
Short name=Beta4Gal-T4
Short name=b4Gal-T4
EC=2.4.1.-
Alternative name(s):
UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 4
UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 4

Including the following 2 domains:

  1. N-acetyllactosamine synthase
    EC=2.4.1.90
    Alternative name(s):
    Nal synthase
  2. Lactotriaosylceramide beta-1,4-galactosyltransferase
    EC=2.4.1.275
    Alternative name(s):
    Beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase
Gene names
Name:B4galt4
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length344 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids By similarity.

Catalytic activity

UDP-alpha-D-galactose + N-acetyl-D-glucosamine = UDP + N-acetyllactosamine.

UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide = UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide.

Cofactor

Manganese By similarity.

Pathway

Protein modification; protein glycosylation.

Subcellular location

Golgi apparatusGolgi stack membrane; Single-pass type II membrane protein By similarity. Note: Trans cisternae of Golgi stack By similarity.

Sequence similarities

Belongs to the glycosyltransferase 7 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 344344Beta-1,4-galactosyltransferase 4
PRO_0000080544

Regions

Topological domain1 – 1212Cytoplasmic Potential
Transmembrane13 – 3826Helical; Signal-anchor for type II membrane protein; Potential
Topological domain39 – 344306Lumenal Potential
Region129 – 1335UDP-alpha-D-galactose binding By similarity
Region168 – 1703UDP-alpha-D-galactose binding By similarity
Region195 – 1962UDP-alpha-D-galactose binding By similarity
Region258 – 2614N-acetyl-D-glucosamine binding By similarity
Region289 – 2913UDP-alpha-D-galactose binding By similarity

Sites

Metal binding1961Manganese By similarity
Metal binding2891Manganese; via tele nitrogen By similarity
Binding site2241UDP-alpha-D-galactose By similarity
Binding site2561UDP-alpha-D-galactose By similarity
Binding site3011N-acetyl-D-glucosamine By similarity

Amino acid modifications

Glycosylation2201N-linked (GlcNAc...) Potential
Glycosylation3351N-linked (GlcNAc...) Potential
Disulfide bond77 ↔ 118 By similarity
Disulfide bond189 ↔ 208 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q66HH1 [UniParc].

Last modified October 11, 2004. Version 1.
Checksum: 864967A3E87A3CC5

FASTA34439,707
        10         20         30         40         50         60 
MGCNPPYLLS YRLRLLLLFT LCLTVLGWAT SNYFVGAIQV IPRAKNFMAT LHKVMYLGNE 

        70         80         90        100        110        120 
ETLGHGAAMK KAELANCPSV SPNLRGQNKL VFKPDLTLEE VQAKNPKVSR GRYRPEECKA 

       130        140        150        160        170        180 
LQRVAVLIPH RNREKHLIYL LEHLHPFLQR QQLDYGIYVI HQTGSKKFNR AKLLNVGYLE 

       190        200        210        220        230        240 
ALKEENWDCF IFHDVDLVPE NDFNLYTCGD QPKHLVVGRN STGYRLRYSK YFGGVTALSR 

       250        260        270        280        290        300 
EQFFKVNGFS NNYWGWGGED DDLRLRVELH KMKISRPKPD VGKYTMIFHT RDKGNEVNGS 

       310        320        330        340 
RMKLLQQMSR VWKTDGLSSC SYRLLSVEHN PLYANITVDF WTAA 

« Hide

References

[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Lung.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC081866 mRNA. Translation: AAH81866.1.
RefSeqNP_001012018.1. NM_001012018.1.
XP_006248428.1. XM_006248366.1.
UniGeneRn.143976.

3D structure databases

ProteinModelPortalQ66HH1.
SMRQ66HH1. Positions 74-344.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000004289.

Protein family/group databases

CAZyGT7. Glycosyltransferase Family 7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000004289; ENSRNOP00000004289; ENSRNOG00000003114.
GeneID303923.
KEGGrno:303923.
UCSCRGD:1307880. rat.

Organism-specific databases

CTD8702.
RGD1307880. B4galt4.

Phylogenomic databases

eggNOGNOG327897.
GeneTreeENSGT00740000115031.
HOGENOMHOG000231027.
HOVERGENHBG058334.
InParanoidQ66HH1.
KOK07969.
OMAHTRDKGN.
OrthoDBEOG7060R0.
PhylomeDBQ66HH1.
TreeFamTF312834.

Enzyme and pathway databases

UniPathwayUPA00378.

Gene expression databases

GenevestigatorQ66HH1.

Family and domain databases

Gene3D3.90.550.10. 1 hit.
InterProIPR003859. Galactosyl_T.
IPR027791. Galactosyl_T_C.
IPR027995. Galactosyl_T_N.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERPTHR19300. PTHR19300. 1 hit.
PfamPF02709. Glyco_transf_7C. 1 hit.
PF13733. Glyco_transf_7N. 1 hit.
[Graphical view]
PRINTSPR02050. B14GALTRFASE.
SUPFAMSSF53448. SSF53448. 1 hit.
ProtoNetSearch...

Other

NextBio652347.
PROQ66HH1.

Entry information

Entry nameB4GT4_RAT
AccessionPrimary (citable) accession number: Q66HH1
Entry history
Integrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: October 11, 2004
Last modified: June 11, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways