Reviewed, UniProtKB/Swiss-Prot Q66HH1 (B4GT4_RAT)

Names and origin

Protein names

Recommended name:
    Beta-1,4-galactosyltransferase 4
      Short name=Beta-1,4-GalTase 4
      Short name=Beta4Gal-T4
      Short name=b4Gal-T4
    EC=2.4.1.-
Alternative name(s):
    UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 4
    UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 4
Including the following 2 domains:
    1- Recommended name:
            N-acetyllactosamine synthase
              EC=2.4.1.90
        Alternative name(s):
            Nal synthetase
    2- Recommended name:
            Beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase
              EC=2.4.1.-

Gene names

Name:

B4galt4

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	344 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids By similarity.
Catalytic activity	UDP-galactose + N-acetyl-D-glucosamine = UDP + N-acetyllactosamine.
Cofactor	Manganese By similarity.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein By similarity. Note: Trans cisternae of Golgi stack By similarity.
Sequence similarities	Belongs to the glycosyltransferase 7 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Ligand	Manganese Metal-binding
Molecular function	Glycosyltransferase Transferase
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	N-acetyllactosamine synthase activity Inferred from electronic annotation. Source: EC manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 344	344	Beta-1,4-galactosyltransferase 4		PRO_0000080544
Regions
	Topological domain	1 – 12	12	Cytoplasmic Potential
	Transmembrane	13 – 38	26	Signal-anchor for type II membrane protein Potential
	Topological domain	39 – 344	306	Lumenal Potential
Sites
	Metal binding	196	1	Manganese By similarity
	Metal binding	289	1	Manganese By similarity
Amino acid modifications
	Glycosylation	220	1	N-linked (GlcNAc...) Potential
	Glycosylation	335	1	N-linked (GlcNAc...) Potential
	Disulfide bond	77 ↔ 118		By similarity
	Disulfide bond	189 ↔ 208		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q66HH1-1 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 864967A3E87A3CC5	FASTA	344	39,707
	10 20 30 40 50 60 MGCNPPYLLS YRLRLLLLFT LCLTVLGWAT SNYFVGAIQV IPRAKNFMAT LHKVMYLGNE 70 80 90 100 110 120 ETLGHGAAMK KAELANCPSV SPNLRGQNKL VFKPDLTLEE VQAKNPKVSR GRYRPEECKA 130 140 150 160 170 180 LQRVAVLIPH RNREKHLIYL LEHLHPFLQR QQLDYGIYVI HQTGSKKFNR AKLLNVGYLE 190 200 210 220 230 240 ALKEENWDCF IFHDVDLVPE NDFNLYTCGD QPKHLVVGRN STGYRLRYSK YFGGVTALSR 250 260 270 280 290 300 EQFFKVNGFS NNYWGWGGED DDLRLRVELH KMKISRPKPD VGKYTMIFHT RDKGNEVNGS 310 320 330 340 RMKLLQQMSR VWKTDGLSSC SYRLLSVEHN PLYANITVDF WTAA

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC081866 mRNA. Translation: AAH81866.1.
IPI	IPI00471883.
RefSeq	NP_001012018.1.
UniGene	Rn.143976
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q66HH1.
Protein family/group databases
CAZy	GT7. Glycosyltransferase Family 7.
Genome annotation databases
Ensembl	ENSRNOT00000004289; ENSRNOP00000004289; ENSRNOG00000003114; Rattus norvegicus. [Genome view]
GeneID	303923.
KEGG	rno:303923.
UCSC	NM_001012018. rat.
Organism-specific databases
CTD	303923.
RGD	1307880. B4galt4.
Phylogenomic databases
HOVERGEN	Q66HH1.
OMA	VWRTDGL.
Enzyme and pathway databases
BRENDA	2.4.1.90. 248.
Gene expression databases
ArrayExpress	Q66HH1.
Genevestigator	Q66HH1.
GermOnline	ENSRNOG00000003114. Rattus norvegicus.
Family and domain databases
InterPro	IPR003859. Galactosyl_T_2. [Graphical view]
PANTHER	PTHR19300. Galactosyl_T_2. 1 hit.
Pfam	PF02709. Galactosyl_T_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	652347.

Entry information

Entry name

B4GT4_RAT

Accession

Primary (citable) accession number: Q66HH1

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2005
Last sequence update:	October 11, 2004
Last modified:	November 3, 2009
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)