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Q66HH1

- B4GT4_RAT

UniProt

Q66HH1 - B4GT4_RAT

Protein

Beta-1,4-galactosyltransferase 4

Gene

B4galt4

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 88 (01 Oct 2014)
      Sequence version 1 (11 Oct 2004)
      Previous versions | rss
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    Functioni

    Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.By similarity

    Catalytic activityi

    UDP-alpha-D-galactose + N-acetyl-D-glucosamine = UDP + N-acetyllactosamine.
    UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide = UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide.

    Cofactori

    Manganese.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi196 – 1961ManganeseBy similarity
    Binding sitei224 – 2241UDP-alpha-D-galactoseBy similarity
    Binding sitei256 – 2561UDP-alpha-D-galactoseBy similarity
    Metal bindingi289 – 2891Manganese; via tele nitrogenBy similarity
    Binding sitei301 – 3011N-acetyl-D-glucosamineBy similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. N-acetyllactosamine synthase activity Source: UniProtKB-EC

    GO - Biological processi

    1. protein glycosylation Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Ligandi

    Manganese, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_196475. Keratan sulfate biosynthesis.
    REACT_199227. N-Glycan antennae elongation.
    UniPathwayiUPA00378.

    Protein family/group databases

    CAZyiGT7. Glycosyltransferase Family 7.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-1,4-galactosyltransferase 4 (EC:2.4.1.-)
    Short name:
    Beta-1,4-GalTase 4
    Short name:
    Beta4Gal-T4
    Short name:
    b4Gal-T4
    Alternative name(s):
    UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 4
    UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 4
    Including the following 2 domains:
    N-acetyllactosamine synthase (EC:2.4.1.90)
    Alternative name(s):
    Nal synthase
    Lactotriaosylceramide beta-1,4-galactosyltransferase (EC:2.4.1.275)
    Alternative name(s):
    Beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase
    Gene namesi
    Name:B4galt4
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 11

    Organism-specific databases

    RGDi1307880. B4galt4.

    Subcellular locationi

    Golgi apparatusGolgi stack membrane By similarity; Single-pass type II membrane protein By similarity
    Note: Trans cisternae of Golgi stack.By similarity

    GO - Cellular componenti

    1. Golgi cisterna membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 344344Beta-1,4-galactosyltransferase 4PRO_0000080544Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi77 ↔ 118By similarity
    Disulfide bondi189 ↔ 208By similarity
    Glycosylationi220 – 2201N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi335 – 3351N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Expressioni

    Gene expression databases

    GenevestigatoriQ66HH1.

    Interactioni

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000004289.

    Structurei

    3D structure databases

    ProteinModelPortaliQ66HH1.
    SMRiQ66HH1. Positions 74-344.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1212CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini39 – 344306LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei13 – 3826Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni129 – 1335UDP-alpha-D-galactose bindingBy similarity
    Regioni168 – 1703UDP-alpha-D-galactose bindingBy similarity
    Regioni195 – 1962UDP-alpha-D-galactose bindingBy similarity
    Regioni258 – 2614N-acetyl-D-glucosamine bindingBy similarity
    Regioni289 – 2913UDP-alpha-D-galactose bindingBy similarity

    Sequence similaritiesi

    Belongs to the glycosyltransferase 7 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG327897.
    GeneTreeiENSGT00740000115031.
    HOGENOMiHOG000231027.
    HOVERGENiHBG058334.
    InParanoidiQ66HH1.
    KOiK07969.
    OMAiHTRDKGN.
    OrthoDBiEOG7060R0.
    PhylomeDBiQ66HH1.
    TreeFamiTF312834.

    Family and domain databases

    Gene3Di3.90.550.10. 1 hit.
    InterProiIPR003859. Galactosyl_T.
    IPR027791. Galactosyl_T_C.
    IPR027995. Galactosyl_T_N.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view]
    PANTHERiPTHR19300. PTHR19300. 1 hit.
    PfamiPF02709. Glyco_transf_7C. 1 hit.
    PF13733. Glyco_transf_7N. 1 hit.
    [Graphical view]
    PRINTSiPR02050. B14GALTRFASE.
    SUPFAMiSSF53448. SSF53448. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q66HH1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGCNPPYLLS YRLRLLLLFT LCLTVLGWAT SNYFVGAIQV IPRAKNFMAT    50
    LHKVMYLGNE ETLGHGAAMK KAELANCPSV SPNLRGQNKL VFKPDLTLEE 100
    VQAKNPKVSR GRYRPEECKA LQRVAVLIPH RNREKHLIYL LEHLHPFLQR 150
    QQLDYGIYVI HQTGSKKFNR AKLLNVGYLE ALKEENWDCF IFHDVDLVPE 200
    NDFNLYTCGD QPKHLVVGRN STGYRLRYSK YFGGVTALSR EQFFKVNGFS 250
    NNYWGWGGED DDLRLRVELH KMKISRPKPD VGKYTMIFHT RDKGNEVNGS 300
    RMKLLQQMSR VWKTDGLSSC SYRLLSVEHN PLYANITVDF WTAA 344
    Length:344
    Mass (Da):39,707
    Last modified:October 11, 2004 - v1
    Checksum:i864967A3E87A3CC5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC081866 mRNA. Translation: AAH81866.1.
    RefSeqiNP_001012018.1. NM_001012018.1.
    XP_006248428.1. XM_006248366.1.
    UniGeneiRn.143976.

    Genome annotation databases

    EnsembliENSRNOT00000004289; ENSRNOP00000004289; ENSRNOG00000003114.
    GeneIDi303923.
    KEGGirno:303923.
    UCSCiRGD:1307880. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC081866 mRNA. Translation: AAH81866.1 .
    RefSeqi NP_001012018.1. NM_001012018.1.
    XP_006248428.1. XM_006248366.1.
    UniGenei Rn.143976.

    3D structure databases

    ProteinModelPortali Q66HH1.
    SMRi Q66HH1. Positions 74-344.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000004289.

    Protein family/group databases

    CAZyi GT7. Glycosyltransferase Family 7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000004289 ; ENSRNOP00000004289 ; ENSRNOG00000003114 .
    GeneIDi 303923.
    KEGGi rno:303923.
    UCSCi RGD:1307880. rat.

    Organism-specific databases

    CTDi 8702.
    RGDi 1307880. B4galt4.

    Phylogenomic databases

    eggNOGi NOG327897.
    GeneTreei ENSGT00740000115031.
    HOGENOMi HOG000231027.
    HOVERGENi HBG058334.
    InParanoidi Q66HH1.
    KOi K07969.
    OMAi HTRDKGN.
    OrthoDBi EOG7060R0.
    PhylomeDBi Q66HH1.
    TreeFami TF312834.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .
    Reactomei REACT_196475. Keratan sulfate biosynthesis.
    REACT_199227. N-Glycan antennae elongation.

    Miscellaneous databases

    NextBioi 652347.
    PROi Q66HH1.

    Gene expression databases

    Genevestigatori Q66HH1.

    Family and domain databases

    Gene3Di 3.90.550.10. 1 hit.
    InterProi IPR003859. Galactosyl_T.
    IPR027791. Galactosyl_T_C.
    IPR027995. Galactosyl_T_N.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view ]
    PANTHERi PTHR19300. PTHR19300. 1 hit.
    Pfami PF02709. Glyco_transf_7C. 1 hit.
    PF13733. Glyco_transf_7N. 1 hit.
    [Graphical view ]
    PRINTSi PR02050. B14GALTRFASE.
    SUPFAMi SSF53448. SSF53448. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Lung.

    Entry informationi

    Entry nameiB4GT4_RAT
    AccessioniPrimary (citable) accession number: Q66HH1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 21, 2005
    Last sequence update: October 11, 2004
    Last modified: October 1, 2014
    This is version 88 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3