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UniProtKB - Q66HG9 (MAVS_RAT)

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Protein

Mitochondrial antiviral-signaling protein

Gene

Mavs

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for innate immune defense against viruses. Acts downstream of DDX58/RIG-I and IFIH1/MDA5, which detect intracellular dsRNA produced during viral replication, to coordinate pathways leading to the activation of NF-kappa-B, IRF3 and IRF7, and to the subsequent induction of antiviral cytokines such as IFN-beta and RANTES (CCL5). Peroxisomal and mitochondrial MAVS act sequentially to create an antiviral cellular state. Upon viral infection, peroxisomal MAVS induces the rapid interferon-independent expression of defense factors that provide short-term protection, whereas mitochondrial MAVS activates an interferon-dependent signaling pathway with delayed kinetics, which amplifies and stabilizes the antiviral response. May activate the same pathways following detection of extracellular dsRNA by TLR3. May protect cells from apoptosis.By similarity

GO - Molecular functioni

Complete GO annotation...

GO - Biological processi

Complete GO annotation...

Keywordsi

Biological processAntiviral defense, Immunity, Innate immunity

Enzyme and pathway databases

ReactomeiR-RNO-936440. Negative regulators of RIG-I/MDA5 signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial antiviral-signaling protein
Short name:
MAVS
Alternative name(s):
Interferon beta promoter stimulator protein 1
Short name:
IPS-1
Virus-induced-signaling adapter
Short name:
VISA
Gene namesi
Name:Mavs
Synonyms:Ips1, Visa
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi1359371. Mavs.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 482CytoplasmicBy similarityAdd BLAST482
Transmembranei483 – 500HelicalSequence analysisAdd BLAST18
Topological domaini501 – 507Mitochondrial intermembraneBy similarity7

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion outer membrane, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001440981 – 507Mitochondrial antiviral-signaling proteinAdd BLAST507

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei152PhosphoserineCombined sources1
Modified residuei157PhosphoserineBy similarity1
Modified residuei172PhosphoserineBy similarity1
Modified residuei178PhosphoserineBy similarity1
Modified residuei186PhosphoserineBy similarity1
Modified residuei220PhosphoserineBy similarity1
Modified residuei234Asymmetric dimethylarginineBy similarity1
Modified residuei256PhosphoserineBy similarity1
Modified residuei387PhosphoserineBy similarity1

Post-translational modificationi

Ubiquitinated. Undergoes 'Lys-48'-linked polyubiquitination catalyzed by ITCH; ITCH-dependent polyubiquitination is mediated by the interaction with PCBP2 and leads to MAVS/IPS1 proteasomal degradation. Ubiquitinated by RNF125, leading to its degradation by the proteasome. Undergoes 'Lys-48'-linked ubiquitination catalyzed by SMURF1.By similarity

Keywords - PTMi

Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ66HG9.
PRIDEiQ66HG9.

PTM databases

iPTMnetiQ66HG9.
PhosphoSitePlusiQ66HG9.

Expressioni

Gene expression databases

BgeeiENSRNOG00000025295.
GenevisibleiQ66HG9. RN.

Interactioni

Subunit structurei

Self-associates and polymerizes (via CARD domains) to form 400 nM long three-stranded helical filaments on mitochondria, filament nucleation requires interaction with DDX58/RIG-I whose CARD domains act as a template for filament assembly. Interacts with DDX58/RIG-I, IFIH1/MDA5, TRAF2, TRAF6 and C1QBP. May interact with IRF3, FADD, RIPK1, IKBKE, CHUK and IKBKB. Interacts with NLRX1. Interaction with NLRX1 requires the CARD domain. Interacts with PSMA7. Interacts with TRAFD1. Interacts (via C-terminus) with PCBP2 in a complex containing MAVS/IPS1, PCBP2 and ITCH. Interacts with CYLD. Interacts with SRC. Interacts with DHX58/LGP2 and IKBKE. Interacts with TMEM173/MITA. Interacts with IFIT3 (via N-terminus). Interacts with TBK1 only in the presence of IFIT3. Interacts with MUL1 (By similarity). Interacts with ANKRD17. Interacts with NDFIP1. Interacts with SMURF1; the interaction is mediated by NDFIP1 and leads to MAVS ubiquitination and degradation.By similarity

GO - Molecular functioni

Complete GO annotation...

Protein-protein interaction databases

IntActiQ66HG9. 1 interactor.
MINTiMINT-2841654.
STRINGi10116.ENSRNOP00000033476.

Structurei

3D structure databases

ProteinModelPortaliQ66HG9.
SMRiQ66HG9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini10 – 77CARDAdd BLAST68

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni10 – 77Required for interaction with NLRX1By similarityAdd BLAST68
Regioni143 – 147Interaction with TRAF2By similarity5
Regioni153 – 158Interaction with TRAF6By similarity6
Regioni435 – 440Interaction with TRAF6By similarity6

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi97 – 233Pro-richAdd BLAST137

Domaini

Both CARD and transmembrane domains are essential for antiviral function. The CARD domain is responsible for interaction with DDX58/RIG-i and IFIH1/MDA5 (By similarity).By similarity
The transmembrane domain and residues 288-424 are essential for its interaction with DHX58/LGP2.By similarity

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IS5U. Eukaryota.
ENOG410Y2HK. LUCA.
GeneTreeiENSGT00510000049120.
HOGENOMiHOG000231697.
HOVERGENiHBG079638.
InParanoidiQ66HG9.
KOiK12648.
OMAiEQDTELG.
OrthoDBiEOG091G040S.
PhylomeDBiQ66HG9.
TreeFamiTF333444.

Family and domain databases

InterProiView protein in InterPro
IPR031964. CARD_dom.
IPR026148. Mt_antiviral_sig_pro.
PANTHERiPTHR21446. PTHR21446. 1 hit.
PfamiView protein in Pfam
PF16739. CARD_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q66HG9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTFAEEKTYK YIRYNHSKFC CVDVLEILPY LSCLTTSDQD RLRASYKQLG 
        60         70         80         90        100
NQGTLWELFN TLQRRPGWVE VFIRALRICE LPGLAEQVTR VYQSYLPPGA 
       110        120        130        140        150
SLHSLDPLQS PRIPTTVSEP SAFAAGHTIP DSGFQDKPGY PKPVQDTQPP 
       160        170        180        190        200
KSPVENSEEP PQANFGAIPR MSGDSLISSP NPPALSPQPS REHPEQEPEL 
       210        220        230        240        250
GGPSTANVDS VPIATYGPVS PTVSFQPLPR IAPRTNLSPG VTVSALSAKT 
       260        270        280        290        300
TLSSSSTGSA FAKGAGDQAK AATCVSTKEG VPTNSVTTSS VPSIKPVPVN 
       310        320        330        340        350
TMSSKLPIST KSTAATPSTV PTNIAPSKLP INSVYTGIVP SKVTASVAKA 
       360        370        380        390        400
SASTMPPERN NKQAKETLEA PATVVTTGSS LTRPDISSRS LHSGPELSKP 
       410        420        430        440        450
GVLVSQVDNE PFSACSMDLA ISPSTSLGSE PNHGPEENEY SSFRIQVDKS 
       460        470        480        490        500
PSVDLLGSPE PLATQQSPEE EEPCASSVSW AKWLGATSAL LAAFLAVMLY 

RSRHLAQ
Length:507
Mass (Da):53,805
Last modified:October 11, 2004 - v1
Checksum:iD2924B1E34CADE47
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC081869 mRNA. Translation: AAH81869.1.
RefSeqiNP_001005556.1. NM_001005556.1.
XP_006235096.1. XM_006235034.3.
XP_006235097.1. XM_006235035.3.
XP_006235098.1. XM_006235036.2.
XP_008760401.1. XM_008762179.2.
UniGeneiRn.34996.

Genome annotation databases

EnsembliENSRNOT00000034146; ENSRNOP00000033476; ENSRNOG00000025295.
ENSRNOT00000090764; ENSRNOP00000073053; ENSRNOG00000025295.
GeneIDi311430.
KEGGirno:311430.
UCSCiRGD:1359371. rat.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiMAVS_RAT
AccessioniPrimary (citable) accession number: Q66HG9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: October 11, 2004
Last modified: May 10, 2017
This is version 109 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome