Mavs

Functionⁱ

Required for innate immune defense against viruses. Acts downstream of DHX33, DDX58/RIG-I and IFIH1/MDA5, which detect intracellular dsRNA produced during viral replication, to coordinate pathways leading to the activation of NF-kappa-B, IRF3 and IRF7, and to the subsequent induction of antiviral cytokines such as IFN-beta and RANTES (CCL5). Peroxisomal and mitochondrial MAVS act sequentially to create an antiviral cellular state. Upon viral infection, peroxisomal MAVS induces the rapid interferon-independent expression of defense factors that provide short-term protection, whereas mitochondrial MAVS activates an interferon-dependent signaling pathway with delayed kinetics, which amplifies and stabilizes the antiviral response. May activate the same pathways following detection of extracellular dsRNA by TLR3. May protect cells from apoptosis (By similarity).By similarity

GO - Molecular functionⁱ

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

activation of innate immune response
Source: RGD
Inferred from sequence orthologyⁱ
- 18818105
cellular response to exogenous dsRNA Source: UniProtKB
defense response to bacterium Source: UniProtKB
defense response to virus
Source: RGD
Inferred from sequence orthologyⁱ
- 20451243
innate immune response Source: UniProtKB
negative regulation of viral genome replication
Source: RGD
Inferred from sequence orthologyⁱ
- 20451243
positive regulation of chemokine (C-C motif) ligand 5 production
Source: RGD
Inferred from sequence orthologyⁱ
- 16127453
positive regulation of defense response to virus by host Source: UniProtKB
positive regulation of DNA binding transcription factor activity
Source: RGD
Inferred from sequence orthologyⁱ
- 16127453
positive regulation of I-kappaB kinase/NF-kappaB signaling
Source: RGD
Inferred from sequence orthologyⁱ
- 12761501
positive regulation of interferon-alpha production Source: UniProtKB
positive regulation of interferon-alpha secretion Source: UniProtKB
positive regulation of interferon-beta production Source: UniProtKB
positive regulation of interferon-beta secretion Source: UniProtKB
positive regulation of interleukin-6 secretion Source: UniProtKB
positive regulation of interleukin-8 production
Source: RGD
Inferred from sequence orthologyⁱ
- 16127453
positive regulation of IP-10 production
Source: RGD
Inferred from sequence orthologyⁱ
- 16127453
positive regulation of protein import into nucleus, translocation
Source: RGD
Inferred from sequence orthologyⁱ
- 18818105
positive regulation of protein phosphorylation
Source: RGD
Inferred from sequence orthologyⁱ
- 16127453
positive regulation of response to cytokine stimulus Source: UniProtKB
positive regulation of transcription by RNA polymerase II
Source: RGD
Inferred from sequence orthologyⁱ
- 18818105
- 16127453
positive regulation of transcription factor import into nucleus
Source: RGD
Inferred from sequence orthologyⁱ
- 18818105
positive regulation of tumor necrosis factor production
Source: RGD
Inferred from sequence orthologyⁱ
- 16127453
positive regulation of tumor necrosis factor secretion Source: UniProtKB
positive regulation of type I interferon-mediated signaling pathway
Source: RGD
Inferred from sequence orthologyⁱ
- 20451243
- 16127453
positive regulation of type I interferon production
Source: RGD
Inferred from sequence orthologyⁱ
- 18780793
regulation of peroxisome organization
Source: RGD
Inferred from sequence orthologyⁱ
- 20451243
RIG-I signaling pathway
Source: RGD
Inferred from sequence orthologyⁱ
- 22745163
signal transduction
Source: RGD
Inferred from sequence orthologyⁱ
- 20451243

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Biological process	Antiviral defense, Immunity, Innate immunity

Biological process

Antiviral defense, Immunity, Innate immunity

Enzyme and pathway databases

Reactomeⁱ	R-RNO-936440 Negative regulators of DDX58/IFIH1 signaling

Reactomeⁱ

R-RNO-936440 Negative regulators of DDX58/IFIH1 signaling

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Mitochondrial antiviral-signaling protein Short name: MAVS Alternative name(s): Interferon beta promoter stimulator protein 1 Short name: IPS-1 Virus-induced-signaling adapter Short name: VISA
Gene namesⁱ	Name:Mavs Synonyms:Ips1, Visa
Organismⁱ	Rattus norvegicus (Rat)
Taxonomic identifierⁱ	10116 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus
Proteomesⁱ	UP000002494 Componentⁱ: Chromosome 3

Organism-specific databases

Rat genome database More...RGDⁱ	1359371 Mavs

RGDⁱ

1359371 Mavs

Topology

Feature key	Position(s)	DescriptionActions	Length
Topological domainⁱ	1 – 482	CytoplasmicBy similarityAdd BLAST	482
Transmembraneⁱ	483 – 500	HelicalSequence analysisAdd BLAST	18
Topological domainⁱ	501 – 507	Mitochondrial intermembraneBy similarity	7

Keywords - Cellular componentⁱ

Membrane, Mitochondrion, Mitochondrion outer membrane, Peroxisome

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000144098	1 – 507	Mitochondrial antiviral-signaling proteinAdd BLAST		507

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	152	PhosphoserineCombined sources	1
Modified residueⁱ	157	PhosphoserineBy similarity	1
Modified residueⁱ	172	PhosphoserineBy similarity	1
Modified residueⁱ	178	PhosphoserineBy similarity	1
Modified residueⁱ	186	PhosphoserineBy similarity	1
Modified residueⁱ	220	PhosphoserineBy similarity	1
Modified residueⁱ	234	Asymmetric dimethylarginineBy similarity	1
Modified residueⁱ	256	PhosphoserineBy similarity	1
Modified residueⁱ	387	PhosphoserineBy similarity	1

Post-translational modificationⁱ

Ubiquitinated. Undergoes 'Lys-48'-linked polyubiquitination catalyzed by ITCH; ITCH-dependent polyubiquitination is mediated by the interaction with PCBP2 and leads to MAVS/IPS1 proteasomal degradation. Ubiquitinated by RNF125, leading to its degradation by the proteasome. Undergoes 'Lys-48'-linked ubiquitination catalyzed by SMURF1.By similarity

Keywords - PTMⁱ

Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbⁱ	Q66HG9
PRIDEⁱ	Q66HG9

PTM databases

iPTMnetⁱ	Q66HG9
PhosphoSitePlusⁱ	Q66HG9

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSRNOG00000025295
Genevisibleⁱ	Q66HG9 RN

Interactionⁱ

Subunit structureⁱ

Self-associates and polymerizes (via CARD domains) to form 400 nM long three-stranded helical filaments on mitochondria, filament nucleation requires interaction with DDX58/RIG-I whose CARD domains act as a template for filament assembly (By similarity). Interacts with DDX58/RIG-I, IFIH1/MDA5, TRAF2, TRAF6 and C1QBP. May interact with IRF3, FADD, RIPK1, IKBKE, CHUK and IKBKB. Interacts with NLRX1. Interaction with NLRX1 requires the CARD domain. Interacts with PSMA7. Interacts with TRAFD1. Interacts (via C-terminus) with PCBP2 in a complex containing MAVS/IPS1, PCBP2 and ITCH. Interacts with CYLD. Interacts with SRC. Interacts with DHX58/LGP2 and IKBKE. Interacts with TMEM173/MITA. Interacts with IFIT3 (via N-terminus). Interacts with TBK1 only in the presence of IFIT3. Interacts with MUL1. Interacts with ANKRD17. Interacts with NDFIP1 (By similarity). Interacts with SMURF1; the interaction is mediated by NDFIP1 and leads to MAVS ubiquitination and degradation (By similarity). Interacts (via C-terminus) with GPATCH3; the interaction is markedly increased upon viral infection (By similarity). Directly interacts (via CARD domain) with ATG5 and ATG12, either as ATG5 and ATG12 monomers or as ATG12-ATG5 conjugates (By similarity). Interacts with DHX33 (via the helicase C-terminal domain) (By similarity).By similarity

GO - Molecular functionⁱ

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

IntActⁱ	Q66HG9 1 interactor.
Molecular INTeraction database More...MINTⁱ	Q66HG9
STRINGⁱ	10116.ENSRNOP00000033476

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q66HG9
SMRⁱ	Q66HG9
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	10 – 77	CARDAdd BLAST		68

Region

Feature key	Position(s)	DescriptionActions	Length
Regionⁱ	10 – 77	Required for interaction with NLRX1By similarityAdd BLAST	68
Regionⁱ	143 – 147	Interaction with TRAF2By similarity	5
Regionⁱ	153 – 158	Interaction with TRAF6By similarity	6
Regionⁱ	340 – 507	Interaction with DHX33By similarityAdd BLAST	168
Regionⁱ	435 – 440	Interaction with TRAF6By similarity	6

Compositional bias

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Compositional biasⁱ	97 – 233	Pro-richAdd BLAST		137

Domainⁱ

Both CARD and transmembrane domains are essential for antiviral function. The CARD domain is responsible for interaction with DDX58/RIG-i and IFIH1/MDA5 (By similarity).By similarity

The transmembrane domain and residues 288-424 are essential for its interaction with DHX58/LGP2.By similarity

Keywords - Domainⁱ

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGⁱ	ENOG410IS5U Eukaryota ENOG410Y2HK LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00510000049120
HOGENOMⁱ	HOG000231697
HOVERGENⁱ	HBG079638
InParanoidⁱ	Q66HG9
KEGG Orthology (KO) More...KOⁱ	K12648
OMAⁱ	EQDTELG
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G040S
PhylomeDBⁱ	Q66HG9
TreeFamⁱ	TF333444

Family and domain databases

InterProⁱ	View protein in InterPro IPR031964 CARD_dom IPR026148 Mt_antiviral_sig_pro
PANTHERⁱ	PTHR21446 PTHR21446, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF16739 CARD_2, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Q66HG9-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MTFAEEKTYK YIRYNHSKFC CVDVLEILPY LSCLTTSDQD RLRASYKQLG 
        60         70         80         90        100
NQGTLWELFN TLQRRPGWVE VFIRALRICE LPGLAEQVTR VYQSYLPPGA 
       110        120        130        140        150
SLHSLDPLQS PRIPTTVSEP SAFAAGHTIP DSGFQDKPGY PKPVQDTQPP 
       160        170        180        190        200
KSPVENSEEP PQANFGAIPR MSGDSLISSP NPPALSPQPS REHPEQEPEL 
       210        220        230        240        250
GGPSTANVDS VPIATYGPVS PTVSFQPLPR IAPRTNLSPG VTVSALSAKT 
       260        270        280        290        300
TLSSSSTGSA FAKGAGDQAK AATCVSTKEG VPTNSVTTSS VPSIKPVPVN 
       310        320        330        340        350
TMSSKLPIST KSTAATPSTV PTNIAPSKLP INSVYTGIVP SKVTASVAKA 
       360        370        380        390        400
SASTMPPERN NKQAKETLEA PATVVTTGSS LTRPDISSRS LHSGPELSKP 
       410        420        430        440        450
GVLVSQVDNE PFSACSMDLA ISPSTSLGSE PNHGPEENEY SSFRIQVDKS 
       460        470        480        490        500
PSVDLLGSPE PLATQQSPEE EEPCASSVSW AKWLGATSAL LAAFLAVMLY 

RSRHLAQ

Length:507

Mass (Da):53,805

Last modified:October 11, 2004 - v1

Checksum:ⁱD2924B1E34CADE47

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	BC081869 mRNA Translation: AAH81869.1
NCBI Reference Sequences More...RefSeqⁱ	NP_001005556.1, NM_001005556.1 XP_006235096.1, XM_006235034.3 XP_006235097.1, XM_006235035.3 XP_006235098.1, XM_006235036.2 XP_008760401.1, XM_008762179.2
UniGeneⁱ	Rn.34996

Genome annotation databases

Ensemblⁱ	ENSRNOT00000034146; ENSRNOP00000033476; ENSRNOG00000025295 ENSRNOT00000090764; ENSRNOP00000073053; ENSRNOG00000025295
GeneIDⁱ	311430
KEGGⁱ	rno:311430
UCSC genome browser More...UCSCⁱ	RGD:1359371 rat

Similar proteinsⁱ

50% Identity

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
Q66HG9	Q8VCF0 Q3U5B1 A0A1S3ERF0 UPI00042D06A7 A0A0U1ZCG0 UPI0003C8C605 A0A1A6H3L8 UPI000644298B UPI0003EDCE36 F6QPU3 +11	Mus musculus (Mouse) Dipodomys ordii (Ord's kangaroo rat) Bubalus bubalis (Domestic water buffalo) Tupaia belangeri (Common tree shrew) (Tupaia glis belangeri) Tupaia chinensis (Chinese tree shrew) Neotoma lepida (Desert woodrat) Otolemur garnettii (Small-eared galago) (Garnett's greater bushbaby) Leptonychotes weddellii (Weddell seal) Equus caballus (Horse) Peromyscus maniculatus bairdii (Prairie deer mouse) And more	503	UniRef50_Q8VCF0	Cluster: Mitochondrial antiviral-signaling protein	22

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	BC081869 mRNA Translation: AAH81869.1
NCBI Reference Sequences More...RefSeqⁱ	NP_001005556.1, NM_001005556.1 XP_006235096.1, XM_006235034.3 XP_006235097.1, XM_006235035.3 XP_006235098.1, XM_006235036.2 XP_008760401.1, XM_008762179.2
UniGeneⁱ	Rn.34996

3D structure databases

ProteinModelPortalⁱ	Q66HG9
SMRⁱ	Q66HG9
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

IntActⁱ	Q66HG9 1 interactor.
Molecular INTeraction database More...MINTⁱ	Q66HG9
STRINGⁱ	10116.ENSRNOP00000033476

PTM databases

iPTMnetⁱ	Q66HG9
PhosphoSitePlusⁱ	Q66HG9

Proteomic databases

PaxDbⁱ	Q66HG9
PRIDEⁱ	Q66HG9

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSRNOT00000034146; ENSRNOP00000033476; ENSRNOG00000025295 ENSRNOT00000090764; ENSRNOP00000073053; ENSRNOG00000025295
GeneIDⁱ	311430
KEGGⁱ	rno:311430
UCSC genome browser More...UCSCⁱ	RGD:1359371 rat

Organism-specific databases

CTDⁱ	57506
Rat genome database More...RGDⁱ	1359371 Mavs

Phylogenomic databases

eggNOGⁱ	ENOG410IS5U Eukaryota ENOG410Y2HK LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00510000049120
HOGENOMⁱ	HOG000231697
HOVERGENⁱ	HBG079638
InParanoidⁱ	Q66HG9
KEGG Orthology (KO) More...KOⁱ	K12648
OMAⁱ	EQDTELG
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G040S
PhylomeDBⁱ	Q66HG9
TreeFamⁱ	TF333444

Enzyme and pathway databases

Reactomeⁱ	R-RNO-936440 Negative regulators of DDX58/IFIH1 signaling

Reactomeⁱ

R-RNO-936440 Negative regulators of DDX58/IFIH1 signaling

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:Q66HG9

PROⁱ

PR:Q66HG9

Gene expression databases

Bgeeⁱ	ENSRNOG00000025295
Genevisibleⁱ	Q66HG9 RN

Family and domain databases

InterProⁱ	View protein in InterPro IPR031964 CARD_dom IPR026148 Mt_antiviral_sig_pro
PANTHERⁱ	PTHR21446 PTHR21446, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF16739 CARD_2, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	MAVS_RAT
Accessionⁱ	Q66HG9Primary (citable) accession number: Q66HG9
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	November 8, 2005
	Last sequence update:	October 11, 2004
	Last modified:	April 25, 2018
	This is version 118 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q66HG9 (MAVS_RAT)

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Mitochondrial antiviral-signaling protein

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Mitochondrion

Peroxisome

Mitochondrion

Peroxisome

Other locations

Topology

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Region

Compositional bias

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ