Reviewed, UniProtKB/Swiss-Prot Q66HG3 (CNDP1_RAT)

Names and origin

Protein names

Recommended name:
    Beta-Ala-His dipeptidase
    EC=3.4.13.20
Alternative name(s):
    Carnosine dipeptidase 1
    CNDP dipeptidase 1

Gene names

Name:	Cndp1
Synonyms:	Cn1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	492 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Preferential hydrolysis of the beta-Ala-|-His dipeptide (carnosine), and also anserine, Xaa-|-His dipeptides and other dipeptides including homocarnosine.
Cofactor	Binds 2 zinc ions per subunit By similarity.
Subunit structure	Homodimer By similarity.
Tissue specificity	Detected exclusively in kidney. Ref.2
Sequence similarities	Belongs to the peptidase M20A family.
Caution	In contrast to human counterpart, it lacks a signal sequence.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Carboxypeptidase Hydrolase Metalloprotease Protease
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Molecular function	carboxypeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW metallopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW protein dimerization activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 492	492	Beta-Ala-His dipeptidase		PRO_0000250531
Sites
	Active site	109	1	By similarity
	Active site	174	1	Proton acceptor By similarity
	Metal binding	107	1	Zinc 2 By similarity
	Metal binding	140	1	Zinc 1 By similarity
	Metal binding	140	1	Zinc 2 By similarity
	Metal binding	175	1	Zinc 1 By similarity
	Metal binding	203	1	Zinc 2 By similarity
	Metal binding	453	1	Zinc 1 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q66HG3-1 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: EF8DAE8C15BF06F5	FASTA	492	54,928
	10 20 30 40 50 60 MLSPPHSGTL EKLFQYIDLH QDEFVQTLKE WVAIESDSVQ PMPRLRQELF RMMALAADKL 70 80 90 100 110 120 RNLGARVDSV DLGSQQMPDG QSLPTPPIIL AELGNDPKKP SVCFYGHLDV QPAQKEDGWL 130 140 150 160 170 180 TDPYTLTEVD GKLYGRGATD NKGPVLAWIN AVSTFRALQQ DLPVNVKFIL EGMEEAGSVA 190 200 210 220 230 240 LEELVKREKD NFFSGVDYIV ISDNLWLSQK KPALTCGTRG NCYFTVEVKC RDQDFHSGTF 250 260 270 280 290 300 GGILNEPMAD LVALLGSLVD SSGHILVPGI YDQMAPITEE EKTMYENIDL DLEEYQKSSR 310 320 330 340 350 360 VERFLFDTKE ELLTHLWRYP SLSIHGIEGA FDEPGTKTVI PGRVLGKFSI RLVPHMTPSV 370 380 390 400 410 420 VETQVTQHLE AVFSKRNSFN KMAVSMVLGL QPWTANINGT QYLAARRAIQ TVFGVDPDMI 430 440 450 460 470 480 QDGSTIPIAK IFQDITQKSV MMLPLGAVDD GEHSQNEKIN RWNYIQGSKL FAAFFLELSK 490 LHSGQQVPSG AF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
[2]	"Sequence identification and characterization of human carnosinase and a closely related non-specific dipeptidase." Teufel M., Saudek V., Ledig J.P., Bernhardt A., Boularand S., Carreau A., Cairns N.J., Carter C., Cowley D.J., Duverger D., Ganzhorn A.J., Guenet C., Heintzelmann B., Laucher V., Sauvage C., Smirnova T. J. Biol. Chem. 278:6521-6531(2003) [PubMed: 12473676] [Abstract] Cited for: TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC081877 mRNA. Translation: AAH81877.1.
IPI	IPI00367063.
RefSeq	NP_001007688.1.
UniGene	Rn.15548
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q66HG3.
Genome annotation databases
Ensembl	ENSRNOT00000034970; ENSRNOP00000034652; ENSRNOG00000027739; Rattus norvegicus. [Genome view]
GeneID	307212.
KEGG	rno:307212.
NMPDR	fig|10116.3.peg.14472.
UCSC	NM_001007687. rat.
Organism-specific databases
CTD	307212.
RGD	1359493. Cndp1.
Phylogenomic databases
HOVERGEN	Q66HG3.
OMA	KDRFFSS.
Enzyme and pathway databases
BRENDA	3.4.13.20. 248.
Gene expression databases
ArrayExpress	Q66HG3.
Genevestigator	Q66HG3.
GermOnline	ENSRNOG00000027739. Rattus norvegicus.
Family and domain databases
InterPro	IPR001261. ArgE/DapE_CS. IPR017153. GSH_degradosome_DUG1. IPR002933. Peptidase_M20. IPR011650. Peptidase_M20_dimer. [Graphical view]
Pfam	PF07687. M20_dimer. 1 hit. PF01546. Peptidase_M20. 1 hit. [Graphical view]
PIRSF	PIRSF037242. CNDP_dipeptidase. 1 hit.
PROSITE	PS00758. ARGE_DAPE_CPG2_1. False negative. PS00759. ARGE_DAPE_CPG2_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	657098.

Entry information

Entry name

CNDP1_RAT

Accession

Primary (citable) accession number: Q66HG3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 3, 2006
Last sequence update:	October 11, 2004
Last modified:	November 3, 2009
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)