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Protein

Leucine-rich repeat flightless-interacting protein 1

Gene

Lrrfip1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional repressor which preferentially binds to the GC-rich consensus sequence (5'-AGCCCCCGGCG-3') and may regulate expression of TNF, EGFR and PDGFA. May control smooth muscle cells proliferation following artery injury through PDGFA repression. May also bind double-stranded RNA. Positively regulates Toll-like receptor (TLR) signaling in response to agonist probably by competing with the negative FLII regulator for MYD88-binding (By similarity).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine-rich repeat flightless-interacting protein 1
Short name:
LRR FLII-interacting protein 1
Gene namesi
Name:Lrrfip1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 9

Organism-specific databases

RGDi1359548. Lrrfip1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 738737Leucine-rich repeat flightless-interacting protein 1PRO_0000248394Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylthreonineBy similarity
Modified residuei16 – 161PhosphoserineBy similarity
Modified residuei83 – 831PhosphoserineBy similarity
Modified residuei84 – 841PhosphoserineBy similarity
Modified residuei88 – 881PhosphoserineBy similarity
Modified residuei92 – 921PhosphoserineBy similarity
Modified residuei302 – 3021PhosphoserineCombined sources
Modified residuei346 – 3461PhosphoserineBy similarity
Modified residuei348 – 3481PhosphoserineBy similarity
Modified residuei551 – 5511PhosphoserineBy similarity
Modified residuei560 – 5601PhosphoserineCombined sources
Modified residuei675 – 6751PhosphoserineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ66HF9.
PRIDEiQ66HF9.

PTM databases

iPTMnetiQ66HF9.
PhosphoSiteiQ66HF9.

Expressioni

Inductioni

Up-regulated after artery wall injury (at protein level).1 Publication

Gene expression databases

GenevisibleiQ66HF9. RN.

Interactioni

Subunit structurei

Homodimer. May also form higher oligomers. Interacts with FLII (By similarity). Interacts with MYD88 (By similarity). Competes with FLII for MyD88-binding, even in the absence of LPS (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000045021.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni479 – 580102DNA-bindingBy similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili94 – 194101By similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi563 – 57614Lys-richAdd
BLAST

Domaini

The DNA-binding domain is intrinsically unstructured.By similarity
The coiled coil mediates dimerization.By similarity

Sequence similaritiesi

Belongs to the LRRFIP family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG2010. Eukaryota.
ENOG4111H1S. LUCA.
GeneTreeiENSGT00530000063564.
HOGENOMiHOG000113391.
HOVERGENiHBG081935.
InParanoidiQ66HF9.
OMAiAREEVGN.
OrthoDBiEOG7DNNTX.
PhylomeDBiQ66HF9.
TreeFamiTF314109.

Family and domain databases

InterProiIPR019139. LRRFIP1/2.
[Graphical view]
PANTHERiPTHR19212. PTHR19212. 1 hit.
PfamiPF09738. DUF2051. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q66HF9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSPEGAQNK EIDCLSPEAQ RLAEARLAAK RAARAEAREI RMKELERQQK
60 70 80 90 100
EVEERPDKDF AEKGSRNMPS LSAATLASLG GTSSRRGSGD TSISMDTEAS
110 120 130 140 150
IREIKDSLAE VEEKYKKAMV SNAQLDNEKT NFMYQVDTLK DMLLELEEQL
160 170 180 190 200
AESQRQYEEK NKEFEREKHA HSILQFQFAE VKEALRQREE MLEKHGIILN
210 220 230 240 250
SEIATNGETS DTVNDVGYQA PTKITKEELN ALKAAGEGTL GKAKEVEVKK
260 270 280 290 300
EIVEKVGQRE TLQDSEQEQP KLNTGKDCVD RGVLHPGEKA ENQRPVEDSA
310 320 330 340 350
LSPGPLAGAK CEQEVQSQDQ ENTSILKSPE QIESHEVTNK SDSRDSNSPE
360 370 380 390 400
PSSCRGGLDS EVSGPTALGI KNQSENSMDS QGKENQEDLG KGSFEPRPDH
410 420 430 440 450
VLGQTPEIDK VSCTDSRGTG GNHLEDVVQA GDTIVEDQVG TMASAEQSKS
460 470 480 490 500
MENHIGRSLN DGLGQSSERE LAHEAAELEE ALTQSSQAGG ENTVTEAEDA
510 520 530 540 550
AVRDEKPLQA DVQATPAAPT VQSGHQDTTG PGSTDTKHTS PHAKERNKAK
560 570 580 590 600
SEQQAEALDS PQKKTKNKKK KNKKKKAAAP METCKDANEE SSCQDPDVGD
610 620 630 640 650
GEEEERVQAT DKKWAAETPE LKEDPQSRPS GKQNDAEEDS GPAEGPTDVL
660 670 680 690 700
DQNSLQCADG DISPVGRKGP QRDASQIGGE EGLVPSQHPG QADEKGIEGH
710 720 730
SVDNSDLSGE LGGFNSESGE QAREEVGNSK SKEDCTMS
Length:738
Mass (Da):80,019
Last modified:October 11, 2004 - v1
Checksum:i10D6F003160948B7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC081883 mRNA. Translation: AAH81883.1.
RefSeqiNP_001014291.1. NM_001014269.1.
UniGeneiRn.153995.

Genome annotation databases

EnsembliENSRNOT00000050191; ENSRNOP00000045021; ENSRNOG00000019892.
GeneIDi367314.
KEGGirno:367314.
UCSCiRGD:1359548. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC081883 mRNA. Translation: AAH81883.1.
RefSeqiNP_001014291.1. NM_001014269.1.
UniGeneiRn.153995.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000045021.

PTM databases

iPTMnetiQ66HF9.
PhosphoSiteiQ66HF9.

Proteomic databases

PaxDbiQ66HF9.
PRIDEiQ66HF9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000050191; ENSRNOP00000045021; ENSRNOG00000019892.
GeneIDi367314.
KEGGirno:367314.
UCSCiRGD:1359548. rat.

Organism-specific databases

CTDi9208.
RGDi1359548. Lrrfip1.

Phylogenomic databases

eggNOGiKOG2010. Eukaryota.
ENOG4111H1S. LUCA.
GeneTreeiENSGT00530000063564.
HOGENOMiHOG000113391.
HOVERGENiHBG081935.
InParanoidiQ66HF9.
OMAiAREEVGN.
OrthoDBiEOG7DNNTX.
PhylomeDBiQ66HF9.
TreeFamiTF314109.

Miscellaneous databases

NextBioi691097.
PROiQ66HF9.

Gene expression databases

GenevisibleiQ66HF9. RN.

Family and domain databases

InterProiIPR019139. LRRFIP1/2.
[Graphical view]
PANTHERiPTHR19212. PTHR19212. 1 hit.
PfamiPF09738. DUF2051. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Brown Norway.
    Tissue: Kidney.
  2. "GC factor 2 represses platelet-derived growth factor A-chain gene transcription and is itself induced by arterial injury."
    Khachigian L.M., Santiago F.S., Rafty L.A., Chan O.L.-W., Delbridge G.J., Bobik A., Collins T., Johnson A.C.
    Circ. Res. 84:1258-1267(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION.
  3. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-302; SER-560 AND SER-675, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiLRRF1_RAT
AccessioniPrimary (citable) accession number: Q66HF9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: October 11, 2004
Last modified: April 13, 2016
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.