Reviewed, UniProtKB/Swiss-Prot Q66HF8 (AL1B1_RAT)

Names and origin

Protein names

Recommended name:
    Aldehyde dehydrogenase X, mitochondrial
    EC=1.2.1.3
Alternative name(s):
    Aldehyde dehydrogenase family 1 member B1

Gene names

Name:	Aldh1b1
Synonyms:	Aldhx

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	519 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	ALDHs play a major role in the detoxification of alcohol-derived acetaldehyde. They are involved in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation By similarity.
Catalytic activity	An aldehyde + NAD+ + H2O = an acid + NADH.
Pathway	Alcohol metabolism; ethanol degradation; acetate from ethanol: step 2/2.
Subunit structure	Homotetramer By similarity.
Subcellular location	Mitochondrion matrix By similarity.
Sequence similarities	Belongs to the aldehyde dehydrogenase family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aldehyde dehydrogenase (NAD) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 19	19	Mitochondrion Potential
	Chain	20 – 519	500	Aldehyde dehydrogenase X, mitochondrial		PRO_0000271413
Regions
	Nucleotide binding	264 – 269	6	NAD By similarity
Sites
	Active site	287	1	Proton acceptor By similarity
	Active site	321	1	Nucleophile By similarity
	Site	188	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q66HF8-1 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 6D25E0E806F8EA50	FASTA	519	57,625
	10 20 30 40 50 60 MLNARFLVPR LLCLQGRTTS YSTAAALPNP IPNPEIRYNQ LFINNEWHDA VSKKTFPTVN 70 80 90 100 110 120 PTTGEVIGHV AEGDRADVDL AVRAAREAFR LGSPWRRMDA SERGRLLNRL ADLVERDRVY 130 140 150 160 170 180 LASLETLDNG KPFQESYVLD LDEVIKVYRY LAGWADKWHG KTIPMDGEHF CFTRHEPVGV 190 200 210 220 230 240 CGQIIPWNFP LVMQGWKLAP ALATGNTVVM KVAEQTPLSA LYLASLIKEA GFPPGVVNII 250 260 270 280 290 300 TGYGPTAGAA IAQHMDVDKV AFTGSTEVGH LIQKAAGDSN LKRVTLELGG KSPSIVLADA 310 320 330 340 350 360 DMDHAVDQCH EALFFNMGQC CCAGSRTFVE ESIYHEFLER TVEKAKKRKV GNPFELDTQQ 370 380 390 400 410 420 GPQVDKEQFE KILGYIRLGQ KEGAKLLCGG ERFGERGFFI KPTVFGNVQD DMRIAREEIF 430 440 450 460 470 480 GPVQPLFKFK KIEEVIQRAN NTRYGLAAAV FTRDLDKALY FSQALQAGTV WVNTYNIVTC 490 500 510 HTPFGGFKES GNGRELGEDG LKAYTEVKTV TIKVSEKNS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
[2]	Lubec G., Afjehi-Sadat L., Chen W.-Q. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 55-75; 162-174; 327-340 AND 444-453, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Hippocampus and Spinal cord.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC081884 mRNA. Translation: AAH81884.1.
IPI	IPI00471872.
RefSeq	NP_001011975.1.
UniGene	Rn.12547
3D structure databases
HSSP	HSSP built from PDB template 1ZUM based on UniProtKB P05091.
SMR	Q66HF8. Positions 27-519.
ModBase	Search...
Protein-protein interaction databases
STRING	Q66HF8.
Genome annotation databases
Ensembl	ENSRNOT00000015281; ENSRNOP00000015282; ENSRNOG00000011497; Rattus norvegicus. [Genome view]
GeneID	298079.
KEGG	rno:298079.
UCSC	NM_001011975. rat.
Organism-specific databases
CTD	298079.
RGD	1306737. Aldh1b1.
Phylogenomic databases
eggNOG	maNOG05395.
HOVERGEN	Q66HF8.
InParanoid	Q66HF8.
PhylomeDB	Q66HF8.
Enzyme and pathway databases
BRENDA	1.2.1.3. 248.
Gene expression databases
ArrayExpress	Q66HF8.
Genevestigator	Q66HF8.
Family and domain databases
InterPro	IPR016161. Ald_DH/histidinol_DH. IPR016160. Ald_DH_CS. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH. [Graphical view]
Gene3D	G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	643122.

Entry information

Entry name

AL1B1_RAT

Accession

Primary (citable) accession number: Q66HF8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 9, 2007
Last sequence update:	October 11, 2004
Last modified:	January 19, 2010
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)