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Q66HF8 (AL1B1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Aldehyde dehydrogenase X, mitochondrial

EC=1.2.1.3
Alternative name(s):
Aldehyde dehydrogenase family 1 member B1
Gene names
Name:Aldh1b1
Synonyms:Aldhx
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length519 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

ALDHs play a major role in the detoxification of alcohol-derived acetaldehyde. They are involved in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation By similarity.

Catalytic activity

An aldehyde + NAD+ + H2O = a carboxylate + NADH.

Pathway

Alcohol metabolism; ethanol degradation; acetate from ethanol: step 2/2.

Subunit structure

Homotetramer By similarity.

Subcellular location

Mitochondrion matrix By similarity.

Sequence similarities

Belongs to the aldehyde dehydrogenase family.

Ontologies

Keywords
   Cellular componentMitochondrion
   DomainTransit peptide
   LigandNAD
   Molecular functionOxidoreductase
   PTMAcetylation
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processethanol catabolic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentmitochondrial matrix

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionaldehyde dehydrogenase (NAD) activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 1919Mitochondrion Potential
Chain20 – 519500Aldehyde dehydrogenase X, mitochondrial
PRO_0000271413

Regions

Nucleotide binding264 – 2696NAD By similarity

Sites

Active site2871Proton acceptor By similarity
Active site3211Nucleophile By similarity
Site1881Transition state stabilizer By similarity

Amino acid modifications

Modified residue531N6-acetyllysine By similarity
Modified residue541N6-acetyllysine; alternate By similarity
Modified residue541N6-succinyllysine; alternate By similarity
Modified residue3661N6-acetyllysine; alternate By similarity
Modified residue3661N6-succinyllysine; alternate By similarity
Modified residue3851N6-acetyllysine; alternate By similarity
Modified residue3851N6-succinyllysine; alternate By similarity
Modified residue4011N6-acetyllysine; alternate By similarity
Modified residue4011N6-succinyllysine; alternate By similarity
Modified residue4281N6-acetyllysine; alternate By similarity
Modified residue4281N6-succinyllysine; alternate By similarity
Modified residue4311N6-acetyllysine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q66HF8 [UniParc].

Last modified October 11, 2004. Version 1.
Checksum: 6D25E0E806F8EA50

FASTA51957,625
        10         20         30         40         50         60 
MLNARFLVPR LLCLQGRTTS YSTAAALPNP IPNPEIRYNQ LFINNEWHDA VSKKTFPTVN 

        70         80         90        100        110        120 
PTTGEVIGHV AEGDRADVDL AVRAAREAFR LGSPWRRMDA SERGRLLNRL ADLVERDRVY 

       130        140        150        160        170        180 
LASLETLDNG KPFQESYVLD LDEVIKVYRY LAGWADKWHG KTIPMDGEHF CFTRHEPVGV 

       190        200        210        220        230        240 
CGQIIPWNFP LVMQGWKLAP ALATGNTVVM KVAEQTPLSA LYLASLIKEA GFPPGVVNII 

       250        260        270        280        290        300 
TGYGPTAGAA IAQHMDVDKV AFTGSTEVGH LIQKAAGDSN LKRVTLELGG KSPSIVLADA 

       310        320        330        340        350        360 
DMDHAVDQCH EALFFNMGQC CCAGSRTFVE ESIYHEFLER TVEKAKKRKV GNPFELDTQQ 

       370        380        390        400        410        420 
GPQVDKEQFE KILGYIRLGQ KEGAKLLCGG ERFGERGFFI KPTVFGNVQD DMRIAREEIF 

       430        440        450        460        470        480 
GPVQPLFKFK KIEEVIQRAN NTRYGLAAAV FTRDLDKALY FSQALQAGTV WVNTYNIVTC 

       490        500        510 
HTPFGGFKES GNGRELGEDG LKAYTEVKTV TIKVSEKNS 

« Hide

References

« Hide 'large scale' references
[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Kidney.
[2]Lubec G., Afjehi-Sadat L., Chen W.-Q.
Submitted (APR-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 55-75; 162-174; 327-340 AND 444-453, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: Sprague-Dawley.
Tissue: Hippocampus and Spinal cord.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC081884 mRNA. Translation: AAH81884.1.
RefSeqNP_001011975.1. NM_001011975.1.
UniGeneRn.12547.

3D structure databases

ProteinModelPortalQ66HF8.
SMRQ66HF8. Positions 27-519.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000015282.

Proteomic databases

PaxDbQ66HF8.
PRIDEQ66HF8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID298079.
KEGGrno:298079.
UCSCRGD:1306737. rat.

Organism-specific databases

CTD219.
RGD1306737. Aldh1b1.

Phylogenomic databases

eggNOGCOG1012.
HOGENOMHOG000271505.
HOVERGENHBG000097.
InParanoidQ66HF8.
KOK00128.
PhylomeDBQ66HF8.

Enzyme and pathway databases

UniPathwayUPA00780; UER00768.

Gene expression databases

GenevestigatorQ66HF8.

Family and domain databases

Gene3D3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
InterProIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
[Graphical view]
PfamPF00171. Aldedh. 1 hit.
[Graphical view]
SUPFAMSSF53720. SSF53720. 1 hit.
PROSITEPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio643122.

Entry information

Entry nameAL1B1_RAT
AccessionPrimary (citable) accession number: Q66HF8
Entry history
Integrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: October 11, 2004
Last modified: April 16, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways