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Q66HF1

- NDUS1_RAT

UniProt

Q66HF1 - NDUS1_RAT

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Protein

NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial

Gene
Ndufs1
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity. This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized By similarity.

Catalytic activityi

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
NADH + acceptor = NAD+ + reduced acceptor.

Cofactori

Binds 1 2Fe-2S cluster per subunit By similarity.
Binds 2 4Fe-4S clusters per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi64 – 641Iron-sulfur (2Fe-2S) By similarity
Metal bindingi75 – 751Iron-sulfur (2Fe-2S) By similarity
Metal bindingi78 – 781Iron-sulfur (2Fe-2S) By similarity
Metal bindingi92 – 921Iron-sulfur (2Fe-2S) By similarity
Metal bindingi124 – 1241Iron-sulfur 2 (4Fe-4S); via pros nitrogen By similarity
Metal bindingi128 – 1281Iron-sulfur 2 (4Fe-4S) By similarity
Metal bindingi131 – 1311Iron-sulfur 2 (4Fe-4S) By similarity
Metal bindingi137 – 1371Iron-sulfur 2 (4Fe-4S) By similarity
Metal bindingi176 – 1761Iron-sulfur 3 (4Fe-4S) By similarity
Metal bindingi179 – 1791Iron-sulfur 3 (4Fe-4S) By similarity
Metal bindingi182 – 1821Iron-sulfur 3 (4Fe-4S) By similarity
Metal bindingi226 – 2261Iron-sulfur 3 (4Fe-4S) By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. electron carrier activity Source: InterPro
  4. metal ion binding Source: UniProtKB-KW
  5. NADH dehydrogenase (ubiquinone) activity Source: RefGenome

GO - Biological processi

  1. apoptotic mitochondrial changes Source: UniProtKB
  2. ATP metabolic process Source: UniProtKB
  3. ATP synthesis coupled electron transport Source: InterPro
  4. cellular respiration Source: UniProtKB
  5. reactive oxygen species metabolic process Source: UniProtKB
  6. regulation of mitochondrial membrane potential Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial (EC:1.6.5.3, EC:1.6.99.3)
Gene namesi
Name:Ndufs1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 9

Organism-specific databases

RGDi1359670. Ndufs1.

Subcellular locationi

Mitochondrion inner membrane By similarity
Note: Matrix and cytoplasmic side of the mitochondrial inner membrane By similarity.

GO - Cellular componenti

  1. mitochondrial intermembrane space Source: UniProtKB
  2. mitochondrial respiratory chain complex I Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2323Mitochondrion By similarityAdd
BLAST
Chaini24 – 727704NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrialPRO_0000271388Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei84 – 841N6-acetyllysine By similarity
Modified residuei499 – 4991N6-acetyllysine By similarity
Modified residuei709 – 7091N6-acetyllysine By similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ66HF1.
PRIDEiQ66HF1.

PTM databases

PhosphoSiteiQ66HF1.

Expressioni

Gene expression databases

GenevestigatoriQ66HF1.

Interactioni

Subunit structurei

Complex I is composed of 45 different subunits By similarity.

Protein-protein interaction databases

BioGridi256969. 1 interaction.
STRINGi10116.ENSRNOP00000015851.

Structurei

3D structure databases

ProteinModelPortaliQ66HF1.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini30 – 108792Fe-2S ferredoxin-typeAdd
BLAST
Domaini245 – 301574Fe-4S Mo/W bis-MGD-typeAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG1034.
GeneTreeiENSGT00390000018768.
HOGENOMiHOG000031442.
HOVERGENiHBG003482.
InParanoidiQ66HF1.
KOiK03934.
OMAiFQGNDVA.
OrthoDBiEOG783MTP.
PhylomeDBiQ66HF1.
TreeFamiTF105756.

Family and domain databases

Gene3Di3.10.20.30. 1 hit.
InterProiIPR001041. 2Fe-2S_ferredoxin-type.
IPR012675. Beta-grasp_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR000283. NADH_UbQ_OxRdtase_75kDa_su_CS.
IPR010228. NADH_UbQ_OxRdtase_Gsu.
IPR019574. NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd.
IPR015405. NuoG_C.
[Graphical view]
PfamiPF09326. DUF1982. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF10588. NADH-G_4Fe-4S_3. 1 hit.
[Graphical view]
SMARTiSM00929. NADH-G_4Fe-4S_3. 1 hit.
[Graphical view]
SUPFAMiSSF54292. SSF54292. 1 hit.
TIGRFAMsiTIGR01973. NuoG. 1 hit.
PROSITEiPS51085. 2FE2S_FER_2. 1 hit.
PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00641. COMPLEX1_75K_1. 1 hit.
PS00642. COMPLEX1_75K_2. 1 hit.
PS00643. COMPLEX1_75K_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q66HF1-1 [UniParc]FASTAAdd to Basket

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MLRIPVKRAL IGLSKSPKGY VRSTGTAASN LIEVFVDGQS VMVEPGTTVL    50
QACEKVGMQI PRFCYHERLS VAGNCRMCLV EIEKAPKVVA ACAMPVMKGW 100
NILTNSEKSK KAREGVMEFL LANHPLDCPI CDQGGECDLQ DQSMMFGSDR 150
SRFLEGKRAV EDKNIGPLVK TIMTRCIQCT RCIRFASEIA GVDDLGTTGR 200
GNDMQVGTYI EKMFMSELSG NIIDICPVGA LTSKPYAFTA RPWETRKTES 250
IDVMDAVGSN IVVSTRTGEV MRILPRMHED INEEWISDKT RFAYDGLKRQ 300
RLTEPMVRNE KGLLTYTSWE DALSRVAGML QSFEGKAVAA IAGGLVDAEA 350
LVALKDLLNK VDSDTLCTEE IFPNEGAGTD LRSNYLLNTT IAGVEEADVV 400
LLVGTNPRFE APLFNARIRK SWLHNDLKVA LIGSPVDLTY RYDHLGDSPK 450
ILQDIASGNH EFSKVLNAAK KPMVVLGSSA LQRDDGAAIL AAVSSIAQKI 500
RVASGAAAEW KVMNILHRIA SQVAALDLGY KPGVEAIRKN PPKLLFLLGA 550
DGGCITRQDL PKDCFIVYQG HHGDVGAPIA DVILPGAAYT EKSATYVNTE 600
GRAQQTKVAV TPPGLAREDW KIIRALSEIA GITLPYDTLD QVRNRLGEVS 650
PNLVRYDDVE EANYFQQASE LAKLVDQEFL ADPLVPPQLT IKDFYMTDSI 700
SRASQTMAKC VKAVTEGAQA VEEPSIC 727
Length:727
Mass (Da):79,412
Last modified:October 11, 2004 - v1
Checksum:iEDAC634B2697984A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC081892 mRNA. Translation: AAH81892.1.
RefSeqiNP_001005550.1. NM_001005550.1.
UniGeneiRn.1467.

Genome annotation databases

EnsembliENSRNOT00000015852; ENSRNOP00000015851; ENSRNOG00000011849.
GeneIDi301458.
KEGGirno:301458.
UCSCiRGD:1359670. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC081892 mRNA. Translation: AAH81892.1 .
RefSeqi NP_001005550.1. NM_001005550.1.
UniGenei Rn.1467.

3D structure databases

ProteinModelPortali Q66HF1.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 256969. 1 interaction.
STRINGi 10116.ENSRNOP00000015851.

PTM databases

PhosphoSitei Q66HF1.

Proteomic databases

PaxDbi Q66HF1.
PRIDEi Q66HF1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000015852 ; ENSRNOP00000015851 ; ENSRNOG00000011849 .
GeneIDi 301458.
KEGGi rno:301458.
UCSCi RGD:1359670. rat.

Organism-specific databases

CTDi 4719.
RGDi 1359670. Ndufs1.

Phylogenomic databases

eggNOGi COG1034.
GeneTreei ENSGT00390000018768.
HOGENOMi HOG000031442.
HOVERGENi HBG003482.
InParanoidi Q66HF1.
KOi K03934.
OMAi FQGNDVA.
OrthoDBi EOG783MTP.
PhylomeDBi Q66HF1.
TreeFami TF105756.

Miscellaneous databases

NextBioi 648791.
PROi Q66HF1.

Gene expression databases

Genevestigatori Q66HF1.

Family and domain databases

Gene3Di 3.10.20.30. 1 hit.
InterProi IPR001041. 2Fe-2S_ferredoxin-type.
IPR012675. Beta-grasp_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR000283. NADH_UbQ_OxRdtase_75kDa_su_CS.
IPR010228. NADH_UbQ_OxRdtase_Gsu.
IPR019574. NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd.
IPR015405. NuoG_C.
[Graphical view ]
Pfami PF09326. DUF1982. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF10588. NADH-G_4Fe-4S_3. 1 hit.
[Graphical view ]
SMARTi SM00929. NADH-G_4Fe-4S_3. 1 hit.
[Graphical view ]
SUPFAMi SSF54292. SSF54292. 1 hit.
TIGRFAMsi TIGR01973. NuoG. 1 hit.
PROSITEi PS51085. 2FE2S_FER_2. 1 hit.
PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00641. COMPLEX1_75K_1. 1 hit.
PS00642. COMPLEX1_75K_2. 1 hit.
PS00643. COMPLEX1_75K_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.
  2. Lubec G., Afjehi-Sadat L., Chen W.-Q.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 185-200; 247-266; 429-441; 484-499; 519-528; 544-557 AND 625-643, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Hippocampus and Spinal cord.

Entry informationi

Entry nameiNDUS1_RAT
AccessioniPrimary (citable) accession number: Q66HF1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: October 11, 2004
Last modified: May 14, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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