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Q66HF1

- NDUS1_RAT

UniProt

Q66HF1 - NDUS1_RAT

Protein

NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial

Gene

Ndufs1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 83 (01 Oct 2014)
      Sequence version 1 (11 Oct 2004)
      Previous versions | rss
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    Functioni

    Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity. This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized By similarity.By similarity

    Catalytic activityi

    NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
    NADH + acceptor = NAD+ + reduced acceptor.

    Cofactori

    Binds 1 2Fe-2S cluster per subunit.By similarity
    Binds 2 4Fe-4S clusters per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi64 – 641Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation
    Metal bindingi75 – 751Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation
    Metal bindingi78 – 781Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation
    Metal bindingi92 – 921Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation
    Metal bindingi124 – 1241Iron-sulfur 2 (4Fe-4S); via pros nitrogenBy similarity
    Metal bindingi128 – 1281Iron-sulfur 2 (4Fe-4S)By similarity
    Metal bindingi131 – 1311Iron-sulfur 2 (4Fe-4S)By similarity
    Metal bindingi137 – 1371Iron-sulfur 2 (4Fe-4S)By similarity
    Metal bindingi176 – 1761Iron-sulfur 3 (4Fe-4S)By similarity
    Metal bindingi179 – 1791Iron-sulfur 3 (4Fe-4S)By similarity
    Metal bindingi182 – 1821Iron-sulfur 3 (4Fe-4S)By similarity
    Metal bindingi226 – 2261Iron-sulfur 3 (4Fe-4S)By similarity

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. electron carrier activity Source: InterPro
    4. metal ion binding Source: UniProtKB-KW
    5. NADH dehydrogenase (ubiquinone) activity Source: RefGenome

    GO - Biological processi

    1. apoptotic mitochondrial changes Source: UniProtKB
    2. ATP metabolic process Source: UniProtKB
    3. ATP synthesis coupled electron transport Source: InterPro
    4. cellular respiration Source: UniProtKB
    5. reactive oxygen species metabolic process Source: UniProtKB
    6. regulation of mitochondrial membrane potential Source: UniProtKB

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Respiratory chain, Transport

    Keywords - Ligandi

    2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial (EC:1.6.5.3, EC:1.6.99.3)
    Gene namesi
    Name:Ndufs1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 9

    Organism-specific databases

    RGDi1359670. Ndufs1.

    Subcellular locationi

    Mitochondrion inner membrane By similarity
    Note: Matrix and cytoplasmic side of the mitochondrial inner membrane.By similarity

    GO - Cellular componenti

    1. mitochondrial intermembrane space Source: UniProtKB
    2. mitochondrial respiratory chain complex I Source: UniProtKB

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion inner membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 2323MitochondrionBy similarityAdd
    BLAST
    Chaini24 – 727704NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrialPRO_0000271388Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei84 – 841N6-acetyllysineBy similarity
    Modified residuei499 – 4991N6-acetyllysineBy similarity
    Modified residuei709 – 7091N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PaxDbiQ66HF1.
    PRIDEiQ66HF1.

    PTM databases

    PhosphoSiteiQ66HF1.

    Expressioni

    Gene expression databases

    GenevestigatoriQ66HF1.

    Interactioni

    Subunit structurei

    Complex I is composed of 45 different subunits.By similarity

    Protein-protein interaction databases

    BioGridi256969. 1 interaction.
    STRINGi10116.ENSRNOP00000015851.

    Structurei

    3D structure databases

    ProteinModelPortaliQ66HF1.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini30 – 108792Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd
    BLAST
    Domaini245 – 301574Fe-4S Mo/W bis-MGD-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the complex I 75 kDa subunit family.Curated
    Contains 1 2Fe-2S ferredoxin-type domain.PROSITE-ProRule annotation
    Contains 1 4Fe-4S Mo/W bis-MGD-type domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG1034.
    GeneTreeiENSGT00390000018768.
    HOGENOMiHOG000031442.
    HOVERGENiHBG003482.
    InParanoidiQ66HF1.
    KOiK03934.
    OMAiFQGNDVA.
    OrthoDBiEOG783MTP.
    PhylomeDBiQ66HF1.
    TreeFamiTF105756.

    Family and domain databases

    Gene3Di3.10.20.30. 1 hit.
    InterProiIPR001041. 2Fe-2S_ferredoxin-type.
    IPR012675. Beta-grasp_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR000283. NADH_UbQ_OxRdtase_75kDa_su_CS.
    IPR010228. NADH_UbQ_OxRdtase_Gsu.
    IPR019574. NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd.
    IPR015405. NuoG_C.
    [Graphical view]
    PfamiPF09326. DUF1982. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF10588. NADH-G_4Fe-4S_3. 1 hit.
    [Graphical view]
    SMARTiSM00929. NADH-G_4Fe-4S_3. 1 hit.
    [Graphical view]
    SUPFAMiSSF54292. SSF54292. 1 hit.
    TIGRFAMsiTIGR01973. NuoG. 1 hit.
    PROSITEiPS51085. 2FE2S_FER_2. 1 hit.
    PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00641. COMPLEX1_75K_1. 1 hit.
    PS00642. COMPLEX1_75K_2. 1 hit.
    PS00643. COMPLEX1_75K_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q66HF1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLRIPVKRAL IGLSKSPKGY VRSTGTAASN LIEVFVDGQS VMVEPGTTVL    50
    QACEKVGMQI PRFCYHERLS VAGNCRMCLV EIEKAPKVVA ACAMPVMKGW 100
    NILTNSEKSK KAREGVMEFL LANHPLDCPI CDQGGECDLQ DQSMMFGSDR 150
    SRFLEGKRAV EDKNIGPLVK TIMTRCIQCT RCIRFASEIA GVDDLGTTGR 200
    GNDMQVGTYI EKMFMSELSG NIIDICPVGA LTSKPYAFTA RPWETRKTES 250
    IDVMDAVGSN IVVSTRTGEV MRILPRMHED INEEWISDKT RFAYDGLKRQ 300
    RLTEPMVRNE KGLLTYTSWE DALSRVAGML QSFEGKAVAA IAGGLVDAEA 350
    LVALKDLLNK VDSDTLCTEE IFPNEGAGTD LRSNYLLNTT IAGVEEADVV 400
    LLVGTNPRFE APLFNARIRK SWLHNDLKVA LIGSPVDLTY RYDHLGDSPK 450
    ILQDIASGNH EFSKVLNAAK KPMVVLGSSA LQRDDGAAIL AAVSSIAQKI 500
    RVASGAAAEW KVMNILHRIA SQVAALDLGY KPGVEAIRKN PPKLLFLLGA 550
    DGGCITRQDL PKDCFIVYQG HHGDVGAPIA DVILPGAAYT EKSATYVNTE 600
    GRAQQTKVAV TPPGLAREDW KIIRALSEIA GITLPYDTLD QVRNRLGEVS 650
    PNLVRYDDVE EANYFQQASE LAKLVDQEFL ADPLVPPQLT IKDFYMTDSI 700
    SRASQTMAKC VKAVTEGAQA VEEPSIC 727
    Length:727
    Mass (Da):79,412
    Last modified:October 11, 2004 - v1
    Checksum:iEDAC634B2697984A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC081892 mRNA. Translation: AAH81892.1.
    RefSeqiNP_001005550.1. NM_001005550.1.
    UniGeneiRn.1467.

    Genome annotation databases

    EnsembliENSRNOT00000015852; ENSRNOP00000015851; ENSRNOG00000011849.
    GeneIDi301458.
    KEGGirno:301458.
    UCSCiRGD:1359670. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC081892 mRNA. Translation: AAH81892.1 .
    RefSeqi NP_001005550.1. NM_001005550.1.
    UniGenei Rn.1467.

    3D structure databases

    ProteinModelPortali Q66HF1.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 256969. 1 interaction.
    STRINGi 10116.ENSRNOP00000015851.

    PTM databases

    PhosphoSitei Q66HF1.

    Proteomic databases

    PaxDbi Q66HF1.
    PRIDEi Q66HF1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000015852 ; ENSRNOP00000015851 ; ENSRNOG00000011849 .
    GeneIDi 301458.
    KEGGi rno:301458.
    UCSCi RGD:1359670. rat.

    Organism-specific databases

    CTDi 4719.
    RGDi 1359670. Ndufs1.

    Phylogenomic databases

    eggNOGi COG1034.
    GeneTreei ENSGT00390000018768.
    HOGENOMi HOG000031442.
    HOVERGENi HBG003482.
    InParanoidi Q66HF1.
    KOi K03934.
    OMAi FQGNDVA.
    OrthoDBi EOG783MTP.
    PhylomeDBi Q66HF1.
    TreeFami TF105756.

    Miscellaneous databases

    NextBioi 648791.
    PROi Q66HF1.

    Gene expression databases

    Genevestigatori Q66HF1.

    Family and domain databases

    Gene3Di 3.10.20.30. 1 hit.
    InterProi IPR001041. 2Fe-2S_ferredoxin-type.
    IPR012675. Beta-grasp_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR000283. NADH_UbQ_OxRdtase_75kDa_su_CS.
    IPR010228. NADH_UbQ_OxRdtase_Gsu.
    IPR019574. NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd.
    IPR015405. NuoG_C.
    [Graphical view ]
    Pfami PF09326. DUF1982. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF10588. NADH-G_4Fe-4S_3. 1 hit.
    [Graphical view ]
    SMARTi SM00929. NADH-G_4Fe-4S_3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54292. SSF54292. 1 hit.
    TIGRFAMsi TIGR01973. NuoG. 1 hit.
    PROSITEi PS51085. 2FE2S_FER_2. 1 hit.
    PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00641. COMPLEX1_75K_1. 1 hit.
    PS00642. COMPLEX1_75K_2. 1 hit.
    PS00643. COMPLEX1_75K_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Kidney.
    2. Lubec G., Afjehi-Sadat L., Chen W.-Q.
      Submitted (APR-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 185-200; 247-266; 429-441; 484-499; 519-528; 544-557 AND 625-643, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: Sprague-Dawley.
      Tissue: Hippocampus and Spinal cord.

    Entry informationi

    Entry nameiNDUS1_RAT
    AccessioniPrimary (citable) accession number: Q66HF1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 9, 2007
    Last sequence update: October 11, 2004
    Last modified: October 1, 2014
    This is version 83 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3