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Protein

NUAK family SNF1-like kinase 2

Gene

Nuak2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Stress-activated kinase involved in tolerance to glucose starvation. Induces cell-cell detachment by increasing F-actin conversion to G-actin. Expression is induced by CD95 or TNF-alpha, via NF-kappa-B. Protects cells from CD95-mediated apoptosis and is required for the increased motility and invasiveness of CD95-activated tumor cells. Able to phosphorylate 'Ser-464' of LATS1 (By similarity).By similarity2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.2 Publications

Cofactori

Mg2+1 Publication

Enzyme regulationi

Activated by phosphorylation on Thr-212 by STK11 in complex with STE20-related adapter-alpha (STRAD alpha) pseudo kinase and CAB39.By similarity2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei85 – 851ATPPROSITE-ProRule annotationBy similarity
Active sitei179 – 1791Proton acceptorPROSITE-ProRule annotationBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi63 – 719ATPPROSITE-ProRule annotationBy similarity

GO - Molecular functioni

  • ATP binding Source: UniProtKB
  • magnesium ion binding Source: UniProtKB
  • protein serine/threonine kinase activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Apoptosis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
NUAK family SNF1-like kinase 2 (EC:2.7.11.1)
Alternative name(s):
SNF1/AMP kinase-related kinase
Short name:
SNARK
Gene namesi
Name:Nuak2Imported
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 13

Organism-specific databases

RGDi1359167. Nuak2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 630630NUAK family SNF1-like kinase 2PRO_0000247759Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei212 – 2121PhosphothreonineBy similarity
Modified residuei529 – 5291PhosphoserineBy similarity
Modified residuei550 – 5501PhosphoserineBy similarity
Modified residuei553 – 5531PhosphoserineBy similarity
Modified residuei579 – 5791PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated at Thr-212 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39 (By similarity). Autophosphorylated in vitro.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ66HE5.
PRIDEiQ66HE5.

PTM databases

PhosphoSiteiQ66HE5.

Expressioni

Tissue specificityi

Expressed in liver, skin, testis, uterus, ovary, adrenal gland and brain (at protein level). Expressed in kidney, heart, skin, spleen, lung, uterus, liver and the exocrine and endocrine compartments of the human pancreas. A kinase-inactive isoform also appears to be expressed in the skin, spleen, lung, uterus, liver and testis.2 Publications

Gene expression databases

GenevisibleiQ66HE5. RN.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000000039.

Structurei

3D structure databases

ProteinModelPortaliQ66HE5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini57 – 307251Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0611. Eukaryota.
ENOG410XPDM. LUCA.
GeneTreeiENSGT00790000122947.
HOGENOMiHOG000004864.
HOVERGENiHBG007160.
InParanoidiQ66HE5.
KOiK08800.
OMAiTDCQEVT.
OrthoDBiEOG7MD4PN.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist. At least one kinase-inactive isoform is believed to be expressed.1 Publication

Isoform 1 (identifier: Q66HE5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MESVALHRRG NLAPSASALA TESARPLADR LIKSPKPLMK KQAVKRHHHK
60 70 80 90 100
HNLRHRYEFL ETLGKGTYGK VKKARESSGR LVAIKSIRKD KIKDEQDLLH
110 120 130 140 150
IRREIEIMSS LNHPHIIAIH EVFENSSKIV IVMEYASRGD LYDYISERPR
160 170 180 190 200
LNERDARHFF RQIVSALHYC HQNGIVHRDL KLENILLDAS GNIKIADFGL
210 220 230 240 250
SNLYHKGKFL QTFCGSPLYA SPEIVNGKPY VGPEVDSWSL GVLLYILVHG
260 270 280 290 300
TMPFDGQDHK TLVKQISSGA YREPCKPSDA CGLIRWLLMV NPIRRATLED
310 320 330 340 350
VASHWWVNWG YSTRIGEQEA LREGGHPSGD SGRASMADWL RRSSRPLLEN
360 370 380 390 400
GAKVCSFFKQ HVPGGGSTGP GLERQHSLKK SRKENDMAQT LQNDPAEDTS
410 420 430 440 450
SRPGKNSLKL PKGILKKKAS PSSGEVQEGP QELRPVSNTP GQPVPAIPLL
460 470 480 490 500
PRKGILKKSR QRESGYYSSP EPSESGELLD AGDVFVSGDP MEQKSPQASG
510 520 530 540 550
RLHRKGILKL NGKFSRTALE GTAPSTFGSL DQLASPHPTA RASRPSGAVS
560 570 580 590 600
EDSILSSESF DQLDLPERLP ETPLRGCVSV DNLRRLEQPP SEGLKRWWQE
610 620 630
SLGDSCFSLT DCQEVTAAYR QALGICSKLS
Length:630
Mass (Da):69,953
Last modified:October 11, 2004 - v1
Checksum:i1626427ABCD5F66E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC081899 mRNA. Translation: AAH81899.1.
RefSeqiNP_001007618.1. NM_001007617.1. [Q66HE5-1]
UniGeneiRn.16922.

Genome annotation databases

EnsembliENSRNOT00000000039; ENSRNOP00000000039; ENSRNOG00000000034. [Q66HE5-1]
GeneIDi289419.
KEGGirno:289419.
UCSCiRGD:1359167. rat. [Q66HE5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC081899 mRNA. Translation: AAH81899.1.
RefSeqiNP_001007618.1. NM_001007617.1. [Q66HE5-1]
UniGeneiRn.16922.

3D structure databases

ProteinModelPortaliQ66HE5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000000039.

PTM databases

PhosphoSiteiQ66HE5.

Proteomic databases

PaxDbiQ66HE5.
PRIDEiQ66HE5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000000039; ENSRNOP00000000039; ENSRNOG00000000034. [Q66HE5-1]
GeneIDi289419.
KEGGirno:289419.
UCSCiRGD:1359167. rat. [Q66HE5-1]

Organism-specific databases

CTDi81788.
RGDi1359167. Nuak2.

Phylogenomic databases

eggNOGiKOG0611. Eukaryota.
ENOG410XPDM. LUCA.
GeneTreeiENSGT00790000122947.
HOGENOMiHOG000004864.
HOVERGENiHBG007160.
InParanoidiQ66HE5.
KOiK08800.
OMAiTDCQEVT.
OrthoDBiEOG7MD4PN.

Miscellaneous databases

PROiQ66HE5.

Gene expression databases

GenevisibleiQ66HE5. RN.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and characterization of a novel sucrose-non-fermenting protein kinase/AMP-activated protein kinase-related protein kinase, SNARK."
    Lefebvre D.L., Bai Y., Shahmolky N., Sharma M., Poon R., Drucker D.J., Rosen C.F.
    Biochem. J. 355:297-305(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, ALTERNATIVE SPLICING, AUTOPHOSPHORYLATION, TISSUE SPECIFICITY.
    Tissue: Keratinocyte1 Publication, Kidney1 Publication and Lung1 Publication.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Kidney.
  3. "Regulation of SNARK activity in response to cellular stresses."
    Lefebvre D.L., Rosen C.F.
    Biochim. Biophys. Acta 1724:71-85(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, ENZYME REGULATION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiNUAK2_RAT
AccessioniPrimary (citable) accession number: Q66HE5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: October 11, 2004
Last modified: July 6, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.