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Protein

Nuclear autoantigenic sperm protein

Gene

Nasp

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for DNA replication, normal cell cycle progression and cell proliferation. Forms a cytoplasmic complex with HSP90 and H1 linker histones and stimulates HSP90 ATPase activity. NASP and H1 histone are subsequently released from the complex and translocate to the nucleus where the histone is released for binding to DNA.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processCell cycle, DNA replication, Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear autoantigenic sperm protein
Short name:
NASP
Gene namesi
Name:NaspImported
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi1359609 Nasp

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002615982 – 776Nuclear autoantigenic sperm proteinAdd BLAST775

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei33N6-acetyllysineBy similarity1
Modified residuei123PhosphothreonineBy similarity1
Modified residuei127PhosphoserineCombined sources1
Modified residuei169PhosphothreonineBy similarity1
Modified residuei175PhosphoserineBy similarity1
Modified residuei188PhosphoserineBy similarity1
Modified residuei241N6-acetyllysineBy similarity1
Modified residuei242PhosphoserineCombined sources1
Modified residuei251N6-acetyllysineBy similarity1
Modified residuei286N6-acetyllysineBy similarity1
Modified residuei306PhosphoserineCombined sources1
Modified residuei321PhosphoserineCombined sources1
Modified residuei379PhosphothreonineCombined sources1
Modified residuei386PhosphoserineBy similarity1
Modified residuei398PhosphoserineCombined sources1
Modified residuei452PhosphothreonineCombined sources1
Modified residuei465PhosphothreonineBy similarity1
Modified residuei468PhosphoserineCombined sources1
Modified residuei482PhosphothreonineCombined sources1
Modified residuei485PhosphoserineCombined sources1
Modified residuei650PhosphoserineBy similarity1
Modified residuei671PhosphothreonineBy similarity1
Modified residuei693PhosphoserineBy similarity1
Modified residuei694PhosphoserineBy similarity1
Modified residuei714PhosphoserineCombined sources1
Cross-linki724Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity
Modified residuei733PhosphoserineBy similarity1
Modified residuei739PhosphoserineBy similarity1
Modified residuei744PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ66HD3
PRIDEiQ66HD3

PTM databases

iPTMnetiQ66HD3
PhosphoSitePlusiQ66HD3

Expressioni

Gene expression databases

BgeeiENSRNOG00000016454
GenevisibleiQ66HD3 RN

Interactioni

Subunit structurei

Binds to linker H1 histones but not to core histones (By similarity). Interacts with histones H2A, H2B, H3 and H4 (By similarity). Also binds to HSP90 in the cytoplasm. This interaction stimulates binding of NASP to HIST1H1T/H1T (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000022170

Structurei

3D structure databases

ProteinModelPortaliQ66HD3
SMRiQ66HD3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati43 – 76TPR 1Sequence analysisAdd BLAST34
Repeati530 – 563TPR 2Sequence analysisAdd BLAST34
Repeati572 – 605TPR 3Sequence analysisAdd BLAST34

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni116 – 127Histone-bindingBy similarityAdd BLAST12
Regioni210 – 242Histone-bindingBy similarityAdd BLAST33
Regioni457 – 500Histone-bindingBy similarityAdd BLAST44

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili126 – 160Sequence analysisAdd BLAST35
Coiled coili191 – 216Sequence analysisAdd BLAST26
Coiled coili597 – 650Sequence analysisAdd BLAST54

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi704 – 710Nuclear localization signalSequence analysis7

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi111 – 646Glu-richSequence analysisAdd BLAST536

Sequence similaritiesi

Belongs to the NASP family.Curated

Keywords - Domaini

Coiled coil, Repeat, TPR repeat

Phylogenomic databases

eggNOGiKOG4563 Eukaryota
ENOG4110P5E LUCA
GeneTreeiENSGT00390000016650
HOGENOMiHOG000013120
HOVERGENiHBG002186
InParanoidiQ66HD3
KOiK11291
OMAiLAETHYH
OrthoDBiEOG091G0PN0
PhylomeDBiQ66HD3
TreeFamiTF317297

Family and domain databases

Gene3Di1.25.40.10, 1 hit
InterProiView protein in InterPro
IPR019544 Tetratricopeptide_SHNi-TPR_dom
IPR013026 TPR-contain_dom
IPR011990 TPR-like_helical_dom_sf
IPR019734 TPR_repeat
PfamiView protein in Pfam
PF10516 SHNi-TPR, 1 hit
SMARTiView protein in SMART
SM00028 TPR, 3 hits
SUPFAMiSSF48452 SSF48452, 1 hit
PROSITEiView protein in PROSITE
PS50005 TPR, 3 hits
PS50293 TPR_REGION, 2 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q66HD3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATESTATAA IAAELVSADK VEDAPAPSTS ADKMESLDVD SEAKKLLGLG
60 70 80 90 100
QKHLVMGDIP AAVNAFQEAA SLLGKKYGET ANECGEAFFF YGKSLLELAR
110 120 130 140 150
MENGVLGNAL EGVHVEEEEG EKTEDESLVE NNDNVDEEAR EELREQVYDA
160 170 180 190 200
MGEKEAKKAE GQSLTKPETD KEQESEVEKG GREDMDISEP AEKLQEKVES
210 220 230 240 250
TSKQLTESSE EAKEAAIPGL NEDEVTSGKT EQESLCTEEG KSISGVYVQN
260 270 280 290 300
KEFREAVPQE EGEEMISLEK KPKETSEDQT IGAPEKQDTL MKVVEIEAEI
310 320 330 340 350
DSEVKSVDVG GEEPKDQGAI SESELGKAVL MQLSGQDVEV SPVVAAEAGS
360 370 380 390 400
EVSEKPGQEI TVLPNNGPVV GQSSAGDQTP SEPQNSAERL SETKDGASVE
410 420 430 440 450
EVKAELVPEQ EEAMPPVEES EAAGDGVETK VAQRATEKAP EDKFKIAANE
460 470 480 490 500
ETQERDEQMK EGEETEGSEE EDKENDKAEE TTNESVLEKK TLQENEEEEI
510 520 530 540 550
GNLELAWDML DLAKIIFKRQ ETKEAQLYAA QAHLKLGEVS VESENYIQAV
560 570 580 590 600
EEFQACLSLQ EQYLEAHDRL LAETHYQLGL AYGYNSQYDE AVAQFGKSID
610 620 630 640 650
VIEKRMAVLL EQMKEAEGSF TEYEKEIEEL KELLPEIREK IEDAKESQRS
660 670 680 690 700
GNVAELALKA TLVESSTSGF TPSGAGASVS MIASRKPTDG ASSSNCVTDI
710 720 730 740 750
SHLVRKKRKP EEESPRKDDA KKAKPEPEVN GGSGDAVSSG NEVSENMEAE
760 770
AENQAESQTT AEGTVDSAAT VKSTAC
Length:776
Mass (Da):84,200
Last modified:October 11, 2004 - v1
Checksum:i8E36041F0B86272F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC081913 mRNA Translation: AAH81913.1
RefSeqiNP_001005543.1, NM_001005543.1
XP_006238708.1, XM_006238646.3
XP_008762215.1, XM_008763993.2
UniGeneiRn.48344

Genome annotation databases

EnsembliENSRNOT00000022170; ENSRNOP00000022170; ENSRNOG00000016454
GeneIDi298441
KEGGirno:298441
UCSCiRGD:1359609 rat

Similar proteinsi

Entry informationi

Entry nameiNASP_RAT
AccessioniPrimary (citable) accession number: Q66HD3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: October 11, 2004
Last modified: May 23, 2018
This is version 111 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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