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Q66HD0

- ENPL_RAT

UniProt

Q66HD0 - ENPL_RAT

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Protein
Endoplasmin
Gene
Hsp90b1, Grp94, Tra1
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Molecular chaperone that functions in the processing and transport of secreted proteins. When associated with CNPY3, required for proper folding of Toll-like receptors. Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity By similarity.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei107 – 1071ATP By similarityBy similarity
Binding sitei149 – 1491ATP By similarityBy similarity
Binding sitei162 – 1621ATP By similarityBy similarity
Binding sitei168 – 1681ATP By similarity
Binding sitei199 – 1991ATP; via amide nitrogen By similarityBy similarity
Binding sitei448 – 4481ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW

GO - Biological processi

  1. ER-associated ubiquitin-dependent protein catabolic process Source: UniProtKB
  2. protein folding Source: InterPro
  3. response to stress Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Ligandi

ATP-binding, Calcium, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_100458. ATF6-alpha activates chaperone genes.
REACT_198631. Trafficking and processing of endosomal TLR.
REACT_198733. Scavenging by Class A Receptors.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoplasmin
Alternative name(s):
94 kDa glucose-regulated protein
Short name:
GRP-94
Heat shock protein 90 kDa beta member 1
Gene namesi
Name:Hsp90b1
Synonyms:Grp94, Tra1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi1310482. Hsp90b1.

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum lumen Source: UniProtKB-SubCell
  2. melanosome Source: UniProtKB-SubCell
  3. smooth endoplasmic reticulum Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121 Reviewed prediction
Add
BLAST
Chaini22 – 804783Endoplasmin
PRO_0000349122Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi62 – 621N-linked (GlcNAc...) Reviewed prediction
Glycosylationi107 – 1071N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi138 – 138Interchain By similarityBy similarity
Glycosylationi217 – 2171N-linked (GlcNAc...) Reviewed prediction
Modified residuei404 – 4041N6-succinyllysine By similarity
Glycosylationi445 – 4451N-linked (GlcNAc...) Reviewed prediction
Modified residuei479 – 4791N6-acetyllysine By similarity
Glycosylationi481 – 4811N-linked (GlcNAc...) Reviewed prediction
Glycosylationi502 – 5021N-linked (GlcNAc...) Reviewed prediction
Modified residuei633 – 6331N6-succinyllysine By similarity

Post-translational modificationi

Phosphorylated By similarity.By similarity

Keywords - PTMi

Acetylation, Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ66HD0.
PRIDEiQ66HD0.

2D gel databases

World-2DPAGE0004:Q66HD0.

Expressioni

Gene expression databases

GenevestigatoriQ66HD0.

Interactioni

Subunit structurei

Homodimer; disulfide-linked. Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5 By similarity. Part of a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX. Interacts with AIMP1; regulates its retention in the endoplasmic reticulum. Interacts with OS9 By similarity. Interacts with CNPY3; this interaction is disrupted in the presence of ATP. Interacts with TLR4, TLR9 and TLR11, but not with TLR3 By similarity. Interacts with MZB1 in a calcium-dependent manner By similarity. Interacts with METTL23 By similarity.By similarity

Protein-protein interaction databases

BioGridi263786. 6 interactions.
IntActiQ66HD0. 7 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ66HD0.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi801 – 8044Prevents secretion from ER

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi287 – 31832Glu-rich
Add
BLAST
Compositional biasi752 – 80352Glu-rich
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0326.
HOGENOMiHOG000031988.
HOVERGENiHBG007374.
KOiK09487.
PhylomeDBiQ66HD0.
TreeFamiTF105969.

Family and domain databases

Gene3Di3.30.565.10. 2 hits.
HAMAPiMF_00505. HSP90.
InterProiIPR003594. HATPase_ATP-bd.
IPR019805. Heat_shock_protein_90_CS.
IPR001404. Hsp90_fam.
IPR020575. Hsp90_N.
IPR020568. Ribosomal_S5_D2-typ_fold.
[Graphical view]
PANTHERiPTHR11528. PTHR11528. 1 hit.
PfamiPF00183. HSP90. 1 hit.
[Graphical view]
PIRSFiPIRSF002583. Hsp90. 1 hit.
PRINTSiPR00775. HEATSHOCK90.
SMARTiSM00387. HATPase_c. 1 hit.
[Graphical view]
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55874. SSF55874. 2 hits.
PROSITEiPS00014. ER_TARGET. 1 hit.
PS00298. HSP90. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q66HD0-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MRVLWVLGLC CVLLTFGFVR ADDEVDVDGT VEEDLGKSRE GSRTDDEVVQ    50
REEEAIQLDG LNASQIRELR EKSEKFAFQA EVNRMMKLII NSLYKNKEIF 100
LRELISNASD ALDKIRLISL TDENALAGNE ELTVKIKCDR EKNLLHVTDT 150
GVGMTREELV KNLGTIAKSG TSEFLNKMTE AQEDGQSTSE LIGQFGVGFY 200
SAFLVADKVI VTSKHNNDTQ HIWESDSNEF SVIADPRGNT LGRGTTITLV 250
LKEEASDYLE LDTIKNLVRK YSQFINFPIY VWSSKTETVE EPLEEDETAQ 300
EEKEEADDEA AVEEEEEEKK PKTKKVEKTV WDWELMNDIK PIWQRPSKEV 350
EEDEYKAFYK SFSKESDDPM AYIHFTAEGE VTFKSILFVP TSAPRGLFDE 400
YGSKKSDYIK LYVRRVFITD DFHDMMPKYL NFVKGVVDSD DLPLNVSRET 450
LQQHKLLKVI RKKLVRKTLD MIKKIADEKY NDTFWKEFGT NIKLGVIEDH 500
SNRTRLAKLL RFQSSHHSTD ITSLDQYVER MKEKQDKIYF MAGSSRKEAE 550
SSPFVERLLK KGYEVIYLTE PVDEYCIQAL PEFDGKRFQN VAKEGVKFDE 600
SEKSKESREA TEKEFEPLLN WMKDKALKDK IEKAVVSQRL TESPCALVAS 650
QYGWSGNMER IMKAQAYQTG KDISTNYYAS QKKTFEINPR HPLIRDMLRR 700
VKEDEDDKTV MDLAVVLFET ATLRSGYLLP DTKAYGDRIE RMLRLSLNID 750
PEAQVEEEPE EEPEDTTEDT TDDSEQDEEE TDAGAEEEEE EQETEKEPTE 800
KDEL 804
Length:804
Mass (Da):92,771
Last modified:September 2, 2008 - v2
Checksum:i5C1A2DC8ED6B1CC8
GO
Isoform 2 (identifier: Q66HD0-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     631-804: IEKAVVSQRL...EKEPTEKDEL → VFGPQTVVSPHRN

Note: No experimental confirmation available.

Show »
Length:643
Mass (Da):74,208
Checksum:i11C636590088ADE9
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei631 – 804174IEKAV…EKDEL → VFGPQTVVSPHRN in isoform 2.
VSP_035206Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CH473960 Genomic DNA. Translation: EDM17052.1.
BC081917 mRNA. Translation: AAH81917.1.
RefSeqiNP_001012197.2. NM_001012197.2. [Q66HD0-1]
UniGeneiRn.101146.

Genome annotation databases

GeneIDi362862.
KEGGirno:362862.
UCSCiRGD:1310482. rat. [Q66HD0-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CH473960 Genomic DNA. Translation: EDM17052.1 .
BC081917 mRNA. Translation: AAH81917.1 .
RefSeqi NP_001012197.2. NM_001012197.2. [Q66HD0-1 ]
UniGenei Rn.101146.

3D structure databases

ProteinModelPortali Q66HD0.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 263786. 6 interactions.
IntActi Q66HD0. 7 interactions.

2D gel databases

World-2DPAGE 0004:Q66HD0.

Proteomic databases

PaxDbi Q66HD0.
PRIDEi Q66HD0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 362862.
KEGGi rno:362862.
UCSCi RGD:1310482. rat. [Q66HD0-1 ]

Organism-specific databases

CTDi 7184.
RGDi 1310482. Hsp90b1.

Phylogenomic databases

eggNOGi COG0326.
HOGENOMi HOG000031988.
HOVERGENi HBG007374.
KOi K09487.
PhylomeDBi Q66HD0.
TreeFami TF105969.

Enzyme and pathway databases

Reactomei REACT_100458. ATF6-alpha activates chaperone genes.
REACT_198631. Trafficking and processing of endosomal TLR.
REACT_198733. Scavenging by Class A Receptors.

Miscellaneous databases

NextBioi 681573.
PROi Q66HD0.

Gene expression databases

Genevestigatori Q66HD0.

Family and domain databases

Gene3Di 3.30.565.10. 2 hits.
HAMAPi MF_00505. HSP90.
InterProi IPR003594. HATPase_ATP-bd.
IPR019805. Heat_shock_protein_90_CS.
IPR001404. Hsp90_fam.
IPR020575. Hsp90_N.
IPR020568. Ribosomal_S5_D2-typ_fold.
[Graphical view ]
PANTHERi PTHR11528. PTHR11528. 1 hit.
Pfami PF00183. HSP90. 1 hit.
[Graphical view ]
PIRSFi PIRSF002583. Hsp90. 1 hit.
PRINTSi PR00775. HEATSHOCK90.
SMARTi SM00387. HATPase_c. 1 hit.
[Graphical view ]
SUPFAMi SSF54211. SSF54211. 1 hit.
SSF55874. SSF55874. 2 hits.
PROSITEi PS00014. ER_TARGET. 1 hit.
PS00298. HSP90. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: Brown Norway.
    Tissue: Testis.
  3. "Proteome profile of the mature rat olfactory bulb."
    Maurya D.K., Sundaram C.S., Bhargava P.
    Proteomics 9:2593-2599(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY (ISOFORM 1), SUBCELLULAR LOCATION.

Entry informationi

Entry nameiENPL_RAT
AccessioniPrimary (citable) accession number: Q66HD0
Secondary accession number(s): A6IFJ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: September 2, 2008
Last modified: September 3, 2014
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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