Reviewed, UniProtKB/Swiss-Prot Q66HC4 (PHOP2_RAT)

Names and origin

Protein names

Recommended name:
    Pyridoxal phosphate phosphatase PHOSPHO2
    EC=3.1.3.74

Gene names

Name:

Phospho2

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	241 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Phosphatase that has high activity toward pyridoxal 5'-phosphate (PLP). Also active at much lower level toward pyrophosphate, phosphoethanolamine (PEA), phosphocholine (PCho), phospho-l-tyrosine, fructose-6-phosphate, p-nitrophenyl phosphate, and h-glycerophosphate By similarity.
Catalytic activity	Pyridoxal 5'-phosphate + H2O = pyridoxal + phosphate.
Cofactor	Magnesium By similarity.
Sequence similarities	Belongs to the HAD-like hydrolase superfamily. PHOSPHO family.

Ontologies

Keywords
Ligand	Magnesium Metal-binding Pyridoxal phosphate
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW pyridoxal phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 241	241	Pyridoxal phosphate phosphatase PHOSPHO2		PRO_0000068835
Sites
	Active site	8	1	Nucleophile By similarity
	Active site	10	1	Proton donor By similarity
	Metal binding	8	1	Magnesium By similarity
	Metal binding	10	1	Magnesium By similarity
	Metal binding	179	1	Magnesium By similarity
	Binding site	19	1	Substrate By similarity
	Binding site	99	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q66HC4-1 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 8BA3CFE909C224CF	FASTA	241	27,627
	10 20 30 40 50 60 MKVLLVFDFD NTIIDDNSDT WIIQCAPDKK LPIELQDSYQ KGLWTEFMGR VFKYLRDEGV 70 80 90 100 110 120 KEEELKRAVT SLPFTSGMIE LLSFLRMNKD RFDCIIISDS NSIFIDWVLE AAAFHDVFDT 130 140 150 160 170 180 VFTNPASFDS TGRLTVRNCH THACTRCPKN LCKNTVLGEF IDKQLQKGVR YTRIVYIGDG 190 200 210 220 230 240 GNDVCPVTFL KKNDVAMPRE GYTLHRTLDK MSQNLEPMAS SIVVWSSGME IISHLQFLIQ M

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC081926 mRNA. Translation: AAH81926.1.
IPI	IPI00195272.
RefSeq	NP_001007643.1.
UniGene	Rn.1266
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	Q66HC4.
Genome annotation databases
Ensembl	ENSRNOG00000007979. Rattus norvegicus. [Contig view]
GeneID	295663.
KEGG	rno:295663.
NMPDR	fig|10116.3.peg.18731.
Organism-specific databases
RGD	1359274. Phospho2.
Phylogenomic databases
HOVERGEN	Q66HC4.
OMA	Q66HC4. NDVAMPR.
Enzyme and pathway databases
BRENDA	3.1.3.74. 248.
Gene expression databases
ArrayExpress	Q66HC4.
GermOnline	ENSRNOG00000007979. Rattus norvegicus.
Family and domain databases
InterPro	IPR006383. HAD-SF_hydro_IB_PSP-like. IPR006384. PyrdxlP_Pase-rel. IPR016965. PyrdxlP_Pase_PHOSPHO2. [Graphical view]
Pfam	PF06888. Put_Phosphatase. 1 hit. [Graphical view]
PIRSF	PIRSF031051. PyrdxlP_Pase_PHOSPHO2. 1 hit.
TIGRFAMs	TIGR01489. DKMTPPase-SF. 1 hit. TIGR01488. HAD-SF-IB. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	639855.

Entry information

Entry name

PHOP2_RAT

Accession

Primary (citable) accession number: Q66HC4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2006
Last sequence update:	October 11, 2004
Last modified:	June 16, 2009
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)