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Protein

Pyridoxal phosphate phosphatase PHOSPHO2

Gene

Phospho2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

Phosphatase that has high activity toward pyridoxal 5'-phosphate (PLP). Also active at much lower level toward pyrophosphate, phosphoethanolamine (PEA), phosphocholine (PCho), phospho-l-tyrosine, fructose-6-phosphate, p-nitrophenyl phosphate, and h-glycerophosphate (By similarity).By similarity

Catalytic activityi

Pyridoxal 5'-phosphate + H2O = pyridoxal + phosphate.

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei8 – 81NucleophileBy similarity
Metal bindingi8 – 81MagnesiumBy similarity
Active sitei10 – 101Proton donorBy similarity
Metal bindingi10 – 101MagnesiumBy similarity
Binding sitei19 – 191SubstrateBy similarity
Binding sitei99 – 991SubstrateBy similarity
Metal bindingi179 – 1791MagnesiumBy similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Pyridoxal phosphate

Names & Taxonomyi

Protein namesi
Recommended name:
Pyridoxal phosphate phosphatase PHOSPHO2 (EC:3.1.3.74)
Gene namesi
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi1359274. Phospho2.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 241241Pyridoxal phosphate phosphatase PHOSPHO2PRO_0000068835Add
BLAST

Proteomic databases

PaxDbiQ66HC4.
PRIDEiQ66HC4.

Expressioni

Gene expression databases

BgeeiENSRNOG00000007979.
GenevisibleiQ66HC4. RN.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000010489.

Structurei

3D structure databases

ProteinModelPortaliQ66HC4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3120. Eukaryota.
ENOG4111NAQ. LUCA.
GeneTreeiENSGT00390000007741.
HOGENOMiHOG000231038.
HOVERGENiHBG080058.
InParanoidiQ66HC4.
KOiK13248.
OMAiASQFECK.
OrthoDBiEOG091G0GZ1.
PhylomeDBiQ66HC4.
TreeFamiTF300112.

Family and domain databases

Gene3Di3.40.50.1000. 2 hits.
InterProiIPR023214. HAD-like_dom.
IPR006383. HAD-SF_hydro_IB_PSP-like.
IPR016965. Pase_PHOSPHO-typ.
IPR006384. PyrdxlP_Pase-rel.
[Graphical view]
PfamiPF06888. Put_Phosphatase. 1 hit.
[Graphical view]
PIRSFiPIRSF031051. PyrdxlP_Pase_PHOSPHO2. 1 hit.
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01489. DKMTPPase-SF. 1 hit.
TIGR01488. HAD-SF-IB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q66HC4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVLLVFDFD NTIIDDNSDT WIIQCAPDKK LPIELQDSYQ KGLWTEFMGR
60 70 80 90 100
VFKYLRDEGV KEEELKRAVT SLPFTSGMIE LLSFLRMNKD RFDCIIISDS
110 120 130 140 150
NSIFIDWVLE AAAFHDVFDT VFTNPASFDS TGRLTVRNCH THACTRCPKN
160 170 180 190 200
LCKNTVLGEF IDKQLQKGVR YTRIVYIGDG GNDVCPVTFL KKNDVAMPRE
210 220 230 240
GYTLHRTLDK MSQNLEPMAS SIVVWSSGME IISHLQFLIQ M
Length:241
Mass (Da):27,627
Last modified:October 11, 2004 - v1
Checksum:i8BA3CFE909C224CF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC081926 mRNA. Translation: AAH81926.1.
RefSeqiNP_001007643.1. NM_001007642.1.
UniGeneiRn.1266.

Genome annotation databases

EnsembliENSRNOT00000010489; ENSRNOP00000010489; ENSRNOG00000007979.
GeneIDi295663.
KEGGirno:295663.
UCSCiRGD:1359274. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC081926 mRNA. Translation: AAH81926.1.
RefSeqiNP_001007643.1. NM_001007642.1.
UniGeneiRn.1266.

3D structure databases

ProteinModelPortaliQ66HC4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000010489.

Proteomic databases

PaxDbiQ66HC4.
PRIDEiQ66HC4.

Protocols and materials databases

DNASUi295663.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000010489; ENSRNOP00000010489; ENSRNOG00000007979.
GeneIDi295663.
KEGGirno:295663.
UCSCiRGD:1359274. rat.

Organism-specific databases

CTDi493911.
RGDi1359274. Phospho2.

Phylogenomic databases

eggNOGiKOG3120. Eukaryota.
ENOG4111NAQ. LUCA.
GeneTreeiENSGT00390000007741.
HOGENOMiHOG000231038.
HOVERGENiHBG080058.
InParanoidiQ66HC4.
KOiK13248.
OMAiASQFECK.
OrthoDBiEOG091G0GZ1.
PhylomeDBiQ66HC4.
TreeFamiTF300112.

Miscellaneous databases

PROiQ66HC4.

Gene expression databases

BgeeiENSRNOG00000007979.
GenevisibleiQ66HC4. RN.

Family and domain databases

Gene3Di3.40.50.1000. 2 hits.
InterProiIPR023214. HAD-like_dom.
IPR006383. HAD-SF_hydro_IB_PSP-like.
IPR016965. Pase_PHOSPHO-typ.
IPR006384. PyrdxlP_Pase-rel.
[Graphical view]
PfamiPF06888. Put_Phosphatase. 1 hit.
[Graphical view]
PIRSFiPIRSF031051. PyrdxlP_Pase_PHOSPHO2. 1 hit.
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01489. DKMTPPase-SF. 1 hit.
TIGR01488. HAD-SF-IB. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPHOP2_RAT
AccessioniPrimary (citable) accession number: Q66HC4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: October 11, 2004
Last modified: September 7, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.