Q66HC4 (PHOP2_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pyridoxal phosphate phosphatase PHOSPHO2
EC=3.1.3.74

Gene names

Name:

Phospho2

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	241 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Phosphatase that has high activity toward pyridoxal 5'-phosphate (PLP). Also active at much lower level toward pyrophosphate, phosphoethanolamine (PEA), phosphocholine (PCho), phospho-l-tyrosine, fructose-6-phosphate, p-nitrophenyl phosphate, and h-glycerophosphate By similarity.
Catalytic activity	Pyridoxal 5'-phosphate + H2O = pyridoxal + phosphate.
Cofactor	Magnesium By similarity.
Sequence similarities	Belongs to the HAD-like hydrolase superfamily. PHOSPHO family.

Ontologies

Keywords
Ligand	Magnesium Metal-binding Pyridoxal phosphate
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW pyridoxal phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 241	241	Pyridoxal phosphate phosphatase PHOSPHO2		PRO_0000068835
Sites
	Active site	8	1	Nucleophile By similarity
	Active site	10	1	Proton donor By similarity
	Metal binding	8	1	Magnesium By similarity
	Metal binding	10	1	Magnesium By similarity
	Metal binding	179	1	Magnesium By similarity
	Binding site	19	1	Substrate By similarity
	Binding site	99	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q66HC4 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 8BA3CFE909C224CF	FASTA	241	27,627
	10 20 30 40 50 60 MKVLLVFDFD NTIIDDNSDT WIIQCAPDKK LPIELQDSYQ KGLWTEFMGR VFKYLRDEGV 70 80 90 100 110 120 KEEELKRAVT SLPFTSGMIE LLSFLRMNKD RFDCIIISDS NSIFIDWVLE AAAFHDVFDT 130 140 150 160 170 180 VFTNPASFDS TGRLTVRNCH THACTRCPKN LCKNTVLGEF IDKQLQKGVR YTRIVYIGDG 190 200 210 220 230 240 GNDVCPVTFL KKNDVAMPRE GYTLHRTLDK MSQNLEPMAS SIVVWSSGME IISHLQFLIQ M

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC081926 mRNA. Translation: AAH81926.1.
IPI	IPI00195272.
RefSeq	NP_001007643.1. NM_001007642.1.
UniGene	Rn.1266.
3D structure databases
ProteinModelPortal	Q66HC4.
ModBase	Search...
Protein-protein interaction databases
STRING	Q66HC4.
Proteomic databases
PRIDE	Q66HC4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000010489; ENSRNOP00000010489; ENSRNOG00000007979.
GeneID	295663.
KEGG	rno:295663.
NMPDR	fig|10116.3.peg.18731.
UCSC	NM_001007642. rat.
Organism-specific databases
CTD	493911.
RGD	1359274. Phospho2.
Phylogenomic databases
eggNOG	maNOG08553.
GeneTree	ENSGT00390000007741.
HOVERGEN	HBG080058.
InParanoid	Q66HC4.
OMA	DWVLEAT.
OrthoDB	EOG4S4PH0.
PhylomeDB	Q66HC4.
Gene expression databases
ArrayExpress	Q66HC4.
Genevestigator	Q66HC4.
GermOnline	ENSRNOG00000007979. Rattus norvegicus.
Family and domain databases
InterPro	IPR023214. HAD-like_dom. IPR006383. HAD-SF_hydro_IB_PSP-like. IPR016965. Pase_PHOSPHO-typ. IPR006384. PyrdxlP_Pase-rel. [Graphical view]
Gene3D	G3DSA:3.40.50.1000. HAD-like_dom. 2 hits.
KO	K13248.
Pfam	PF06888. Put_Phosphatase. 1 hit. [Graphical view]
PIRSF	PIRSF031051. PyrdxlP_Pase_PHOSPHO2. 1 hit.
SUPFAM	SSF56784. HAD-like_dom. 1 hit.
TIGRFAMs	TIGR01489. DKMTPPase-SF. 1 hit. TIGR01488. HAD-SF-IB. 1 hit.
ProtoNet	Search...
Other
NextBio	639855.

Entry information

Entry name

PHOP2_RAT

Accession

Primary (citable) accession number: Q66HC4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2006
Last sequence update:	October 11, 2004
Last modified:	November 16, 2011
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program