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Protein

Cyclic AMP-responsive element-binding protein 3-like protein 1

Gene

Creb3l1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Transcription factor involved in unfolded protein response (UPR). In the absence of endoplasmic reticulum (ER) stress, inserted into ER membranes, with N-terminal DNA-binding and transcription activation domains oriented toward the cytosolic face of the membrane. In response to ER stress, transported to the Golgi, where it is cleaved in a site-specific manner by resident proteases S1P/MBTPS1 and S2P/MBTPS2. The released N-terminal cytosolic domain is translocated to the nucleus to effect transcription of specific target genes. Plays a critical role in bone formation through the transcription of COL1A1, and possibly COL1A2, and the secretion of bone matrix proteins. Directly binds to the UPR element (UPRE)-like sequence in an osteoblast-specific COL1A1 promoter region and induces its transcription. Does not regulate COL1A1 in other tissues, such as skin. Required to protect astrocytes from ER stress-induced cell death. In astrocytes, binds to the cAMP response element (CRE) of the BiP/HSPA5 promoter and participate in its transcriptional activation (By similarity). Inhibits cell-cycle progression by binding to promoters and activating transcription of genes encoding cell-cycle inhibitors, such as p21/CDKN1A. Binds the DNA consensus sequence 5'-GTGXGCXGC-3'.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator, Developmental protein

Keywords - Biological processi

Transcription, Transcription regulation, Unfolded protein response

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclic AMP-responsive element-binding protein 3-like protein 1
Short name:
cAMP-responsive element-binding protein 3-like protein 1
Alternative name(s):
Old astrocyte specifically-induced substance
Short name:
OASIS
Cleaved into the following chain:
Gene namesi
Name:Creb3l1
Synonyms:Oasis
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi1359613. Creb3l1.

Subcellular locationi

  • Endoplasmic reticulum membrane; Single-pass type II membrane protein

  • Note: ER membrane resident protein. Upon ER stress, translocated to the Golgi apparatus where it is cleaved. The cytosolic N-terminal fragment (processed cyclic AMP-responsive element-binding protein 3-like protein 1) is transported into the nucleus.By similarity
Processed cyclic AMP-responsive element-binding protein 3-like protein 1 :
  • Nucleus

  • Note: Upon ER stress, translocated into the nucleus.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 375375CytoplasmicSequence analysisAdd
BLAST
Transmembranei376 – 39621Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini397 – 520124LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 520520Cyclic AMP-responsive element-binding protein 3-like protein 1PRO_0000288066Add
BLAST
Chaini1 – ?Processed cyclic AMP-responsive element-binding protein 3-like protein 1PRO_0000296208

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi498 – 4981N-linked (GlcNAc...)Sequence analysis
Glycosylationi514 – 5141N-linked (GlcNAc...)Sequence analysis

Post-translational modificationi

Upon ER stress, translocated to the Golgi apparatus, where it is processed by regulated intramembrane proteolysis (RIP) to release the cytosol-facing N-terminal transcription factor domain. The cleavage is performed sequentially by site-1 and site-2 proteases (S1P/MBTPS1 and S2P/MBTPS2).By similarity
N-glycosylated.By similarity
Ubiquitinated by HRD1/SYVN1; undergoes 'Lys-48'-linked ubiquitination, followed by rapid proteasomal degradation under normal conditions. Upon ER stress, SYVN1 E3 ubiquitin-protein ligase dissociates from its substrate, ubiquitination does not occur and CREB3L1 is stabilized.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei425 – 4262Cleavage; by S1PBy similarity

Keywords - PTMi

Glycoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ66HA2.
PRIDEiQ66HA2.

Expressioni

Gene expression databases

GenevisibleiQ66HA2. RN.

Interactioni

Protein-protein interaction databases

BioGridi263234. 2 interactions.
STRINGi10116.ENSRNOP00000007771.

Structurei

3D structure databases

ProteinModelPortaliQ66HA2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini289 – 35264bZIPPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 6060Required for transcription activationBy similarityAdd
BLAST
Regioni291 – 32030Basic motifPROSITE-ProRule annotationAdd
BLAST
Regioni331 – 35222Leucine-zipperPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi391 – 3944S2P recognitionBy similarity
Motifi422 – 4254S1P recognitionBy similarity

Sequence similaritiesi

Belongs to the bZIP family. ATF subfamily.Curated
Contains 1 bZIP (basic-leucine zipper) domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0709. Eukaryota.
ENOG410ZZQM. LUCA.
GeneTreeiENSGT00520000055538.
HOGENOMiHOG000060150.
HOVERGENiHBG057480.
InParanoidiQ66HA2.
KOiK09048.
OMAiEPPDGWE.
OrthoDBiEOG7327QN.
PhylomeDBiQ66HA2.
TreeFamiTF316079.

Family and domain databases

InterProiIPR004827. bZIP.
IPR001630. Leuzip_CREB.
IPR029805. OASIS.
[Graphical view]
PANTHERiPTHR22952:SF24. PTHR22952:SF24. 2 hits.
PfamiPF00170. bZIP_1. 1 hit.
[Graphical view]
PRINTSiPR00041. LEUZIPPRCREB.
SMARTiSM00338. BRLZ. 1 hit.
[Graphical view]
PROSITEiPS50217. BZIP. 1 hit.
PS00036. BZIP_BASIC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q66HA2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDAVLEPFPA DRLFPGSSFL DLGDLNESDF LNNAHFPEHL DHFVENMEDF
60 70 80 90 100
SNDLFSSFFD DPVLDEKSPL LDMELDSPAP GIQAEHSYSL SGDSAPQSPL
110 120 130 140 150
VPVKMEDTTQ DMEHGAWALG NKLCSIMVKQ EQSPELPVDP LAASSAMAAA
160 170 180 190 200
TMATPPLLGL SPISRLPIPH QAPGEMTQLP VIKAEPPEMS QFLKVTQEDL
210 220 230 240 250
VQMPPTPPSS HGSDSDGSQS PRSLPPSSPV RPMARSSTAI STSPLLTAPH
260 270 280 290 300
KLQGTSGPLL LTEEEKRTLI AEGYPIPTKL PLTKAEEKAL KRVRRKIKNK
310 320 330 340 350
ISAQESRRKK KEYVECLEKK VETYTSENNE LWKKVETLET ANRTLLQQLQ
360 370 380 390 400
KLQTLVTSKI SRPYKMAATQ TGTCLMVAAL CFVLVLGSLA PCLPAFSSGS
410 420 430 440 450
KTVKEDPVAA DSVYAASQMP SRSLLFYDDG AGSWEDGHRG ALLPVEPPEG
460 470 480 490 500
WELKPGGPAE PRPQDHLRHD HADSIHETTK YLRETWPEDT EDNGASPNFS
510 520
HPKEWFHDRD LGPNTTIKLS
Length:520
Mass (Da):57,134
Last modified:October 11, 2004 - v1
Checksum:iE23974CC13932863
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC081951 mRNA. Translation: AAH81951.1.
RefSeqiNP_001005562.1. NM_001005562.1.
UniGeneiRn.24541.

Genome annotation databases

EnsembliENSRNOT00000007771; ENSRNOP00000007771; ENSRNOG00000005413.
GeneIDi362165.
KEGGirno:362165.
UCSCiRGD:1359613. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC081951 mRNA. Translation: AAH81951.1.
RefSeqiNP_001005562.1. NM_001005562.1.
UniGeneiRn.24541.

3D structure databases

ProteinModelPortaliQ66HA2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi263234. 2 interactions.
STRINGi10116.ENSRNOP00000007771.

Proteomic databases

PaxDbiQ66HA2.
PRIDEiQ66HA2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000007771; ENSRNOP00000007771; ENSRNOG00000005413.
GeneIDi362165.
KEGGirno:362165.
UCSCiRGD:1359613. rat.

Organism-specific databases

CTDi90993.
RGDi1359613. Creb3l1.

Phylogenomic databases

eggNOGiKOG0709. Eukaryota.
ENOG410ZZQM. LUCA.
GeneTreeiENSGT00520000055538.
HOGENOMiHOG000060150.
HOVERGENiHBG057480.
InParanoidiQ66HA2.
KOiK09048.
OMAiEPPDGWE.
OrthoDBiEOG7327QN.
PhylomeDBiQ66HA2.
TreeFamiTF316079.

Miscellaneous databases

NextBioi678864.
PROiQ66HA2.

Gene expression databases

GenevisibleiQ66HA2. RN.

Family and domain databases

InterProiIPR004827. bZIP.
IPR001630. Leuzip_CREB.
IPR029805. OASIS.
[Graphical view]
PANTHERiPTHR22952:SF24. PTHR22952:SF24. 2 hits.
PfamiPF00170. bZIP_1. 1 hit.
[Graphical view]
PRINTSiPR00041. LEUZIPPRCREB.
SMARTiSM00338. BRLZ. 1 hit.
[Graphical view]
PROSITEiPS50217. BZIP. 1 hit.
PS00036. BZIP_BASIC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lung.

Entry informationi

Entry nameiCR3L1_RAT
AccessioniPrimary (citable) accession number: Q66HA2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: October 11, 2004
Last modified: May 11, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.