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Protein

2-phosphoxylose phosphatase 1

Gene

Pxylp1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Responsible for the 2-O-dephosphorylation of xylose in the glycosaminoglycan-protein linkage region of proteoglycans thereby regulating the amount of mature glycosaminoglycan (GAG) chains. Sulfated glycosaminoglycans (GAGs), including heparan sulfate and chondroitin sulfate, are synthesized on the so-called common GAG-protein linkage region (GlcUAbeta1-3Galbeta1-3Galbeta1-4Xylbeta1-O-Ser) of core proteins, which is formed by the stepwise addition of monosaccharide residues by the respective specific glycosyltransferases. Xylose 2-O-dephosphorylation during completion of linkage region formation is a prerequisite for the initiation and efficient elongation of the repeating disaccharide region of GAG chains.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei97 – 971NucleophileBy similarity
Active sitei379 – 3791Proton donorBy similarity

GO - Molecular functioni

  1. acid phosphatase activity Source: InterPro
  2. phosphatase activity Source: UniProtKB

GO - Biological processi

  1. chondroitin sulfate proteoglycan biosynthetic process Source: UniProtKB
  2. dephosphorylation Source: GOC
  3. glycosaminoglycan biosynthetic process Source: UniProtKB
  4. positive regulation of heparan sulfate proteoglycan biosynthetic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
2-phosphoxylose phosphatase 1By similarity (EC:3.1.3.-By similarity)
Alternative name(s):
Acid phosphatase-like protein 2
Gene namesi
Name:Pxylp1Imported
Synonyms:Acpl2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 8

Organism-specific databases

RGDi1359617. Pxylp1.

Subcellular locationi

  1. Golgi apparatus membrane By similarity; Single-pass type II membrane protein Sequence Analysis

  2. Note: Colocalizes to Golgi apparatus in a B3GAT3-dependent manner.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 66CytoplasmicSequence Analysis
Transmembranei7 – 2721Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini28 – 480453LumenalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. Golgi apparatus Source: UniProtKB
  2. Golgi membrane Source: UniProtKB-SubCell
  3. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 4804802-phosphoxylose phosphatase 1PRO_0000314926Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi194 – 1941N-linked (GlcNAc...)PROSITE-ProRule annotation
Glycosylationi305 – 3051N-linked (GlcNAc...)Sequence Analysis
Glycosylationi354 – 3541N-linked (GlcNAc...)PROSITE-ProRule annotation

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiQ66H78.

Expressioni

Gene expression databases

GenevestigatoriQ66H78.

Interactioni

Subunit structurei

Interacts with B3GAT3; the interaction increases the 2-phosphoxylose phosphatase activity of PXYLP1 during completion of linkage region formation in a B3GAT3-mediated manner.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000017085.

Structurei

3D structure databases

ProteinModelPortaliQ66H78.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the histidine acid phosphatase family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG321954.
GeneTreeiENSGT00390000016324.
HOGENOMiHOG000033787.
HOVERGENiHBG106056.
InParanoidiQ66H78.
OMAiFDWKKIY.
OrthoDBiEOG747PJ2.
PhylomeDBiQ66H78.
TreeFamiTF318821.

Family and domain databases

Gene3Di3.40.50.1240. 2 hits.
InterProiIPR000560. His_Pase_superF_clade-2.
IPR029033. His_PPase_superfam.
[Graphical view]
PfamiPF00328. His_Phos_2. 1 hit.
[Graphical view]
SUPFAMiSSF53254. SSF53254. 1 hit.

Sequencei

Sequence statusi: Complete.

Q66H78-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLYRNRFLVL LALAGLLAFL SLSLQFFHLI PVSTTKNGGS SKSRKRIMPD
60 70 80 90 100
PVTEPPTVDP VYEALLYCNI PSVAEHSMEG HAPHHYKLVS VHVFIRHGDR
110 120 130 140 150
YPLYAIPKTK RPEIDCTLVA SRKPYHPKLE AFVGHMLKGS GASFESPLGS
160 170 180 190 200
LPLYPNHPLC EMGELTQTGV VQHLQNGQLL RDIYLRKHKL LPNNWSSDQL
210 220 230 240 250
YLETTGKSRT LQSGLALLYG FLPEFDWKKV YFKHQPSALF CSGSCYCPLR
260 270 280 290 300
NQYLEKEQRR QYLLRLKNSD LERTYGEMAK IVDIPTKQLR AANPIDSMLC
310 320 330 340 350
HFCHNVSFPC SRSGCLGMEH FKVIKTHQIE DERERHEKLL YFGYSLLGAH
360 370 380 390 400
PILNQTVNRM QRAALGWRDE LFTLYSAHDV TLSPILSALG LLEARFPRFA
410 420 430 440 450
ARLVFELWQD RQKPSEHSVR ILYNGADVTF HTSFCHDFHK HSPKPMCPLE
460 470 480
NLVRFVKRDM FVALDGSSTN YYDACHGEGA
Length:480
Mass (Da):54,958
Last modified:October 11, 2004 - v1
Checksum:i1A3168B91164A14E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC081981 mRNA. Translation: AAH81981.1.
RefSeqiNP_001007711.1. NM_001007710.1.
XP_006243653.1. XM_006243591.2.
XP_006243654.1. XM_006243592.2.
UniGeneiRn.9589.

Genome annotation databases

EnsembliENSRNOT00000017085; ENSRNOP00000017085; ENSRNOG00000012480.
GeneIDi315939.
KEGGirno:315939.
UCSCiRGD:1359617. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC081981 mRNA. Translation: AAH81981.1.
RefSeqiNP_001007711.1. NM_001007710.1.
XP_006243653.1. XM_006243591.2.
XP_006243654.1. XM_006243592.2.
UniGeneiRn.9589.

3D structure databases

ProteinModelPortaliQ66H78.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000017085.

Proteomic databases

PRIDEiQ66H78.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000017085; ENSRNOP00000017085; ENSRNOG00000012480.
GeneIDi315939.
KEGGirno:315939.
UCSCiRGD:1359617. rat.

Organism-specific databases

CTDi92370.
RGDi1359617. Pxylp1.

Phylogenomic databases

eggNOGiNOG321954.
GeneTreeiENSGT00390000016324.
HOGENOMiHOG000033787.
HOVERGENiHBG106056.
InParanoidiQ66H78.
OMAiFDWKKIY.
OrthoDBiEOG747PJ2.
PhylomeDBiQ66H78.
TreeFamiTF318821.

Miscellaneous databases

NextBioi670088.
PROiQ66H78.

Gene expression databases

GenevestigatoriQ66H78.

Family and domain databases

Gene3Di3.40.50.1240. 2 hits.
InterProiIPR000560. His_Pase_superF_clade-2.
IPR029033. His_PPase_superfam.
[Graphical view]
PfamiPF00328. His_Phos_2. 1 hit.
[Graphical view]
SUPFAMiSSF53254. SSF53254. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.

Entry informationi

Entry nameiPXYP1_RAT
AccessioniPrimary (citable) accession number: Q66H78
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 11, 2004
Last modified: February 4, 2015
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.