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Protein

Paxillin

Gene

Pxn

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).By similarity

GO - Molecular functioni

  • beta-catenin binding Source: RGD
  • BH4 domain binding Source: RGD
  • integrin binding Source: RGD
  • metal ion binding Source: UniProtKB-KW
  • neuropilin binding Source: RGD
  • protein complex binding Source: RGD
  • protein kinase binding Source: RGD
  • ubiquitin protein ligase binding Source: RGD
  • vinculin binding Source: RGD

GO - Biological processi

Keywordsi

Biological processCell adhesion
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-RNO-180292. GAB1 signalosome.
R-RNO-4420097. VEGFA-VEGFR2 Pathway.
R-RNO-445355. Smooth Muscle Contraction.
R-RNO-8849471. PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases.

Names & Taxonomyi

Protein namesi
Recommended name:
Paxillin
Gene namesi
Name:Pxn
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 12

Organism-specific databases

RGDi1305759. Pxn.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cytoplasm, Cytoskeleton

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL5613.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000758561 – 586PaxillinAdd BLAST586

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei31Phosphotyrosine; by PTK6By similarity1
Modified residuei83PhosphoserineCombined sources1
Modified residuei85PhosphoserineBy similarity1
Modified residuei88PhosphotyrosineBy similarity1
Modified residuei106PhosphoserineBy similarity1
Modified residuei118Phosphotyrosine; by PTK6By similarity1
Modified residuei119PhosphoserineBy similarity1
Modified residuei126PhosphoserineCombined sources1
Modified residuei130PhosphoserineCombined sources1
Modified residuei132PhosphothreonineBy similarity1
Modified residuei137PhosphoserineBy similarity1
Modified residuei140PhosphoserineBy similarity1
Modified residuei143PhosphoserineCombined sources1
Modified residuei210PhosphotyrosineBy similarity1
Modified residuei259PhosphoserineCombined sources1
Modified residuei273Phosphoserine; by CDK5By similarity1
Modified residuei279PhosphoserineBy similarity1
Modified residuei287PhosphoserineCombined sources1
Modified residuei290PhosphoserineCombined sources1
Modified residuei301PhosphoserineBy similarity1
Modified residuei317PhosphoserineBy similarity1
Modified residuei327PhosphoserineCombined sources1
Modified residuei335PhosphoserineCombined sources1
Modified residuei528PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated by MAPK1/ERK2 (By similarity). Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens. Phosphorylation at Ser-273 by CDK5 reduces its interaction with PTK2/FAK1 in matrix-cell focal adhesions (MCFA) during oligodendrocytes (OLs) differentiation (By similarity). Phosphorylation at Tyr-31 and Tyr-118 by PTK6 promote the activation of RAC1 via CRK/CrKII, thereby promoting migration and invasion (By similarity). Phosphorylation at Ser-279 by SLK is required for PXN redistribution and cell motility (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ66H76.
PRIDEiQ66H76.

PTM databases

iPTMnetiQ66H76.
PhosphoSitePlusiQ66H76.

Expressioni

Gene expression databases

BgeeiENSRNOG00000001149.
ExpressionAtlasiQ66H76. baseline and differential.
GenevisibleiQ66H76. RN.

Interactioni

Subunit structurei

Binds to vinculin and to the SH3 domain of SRC. Interacts with GIT1 and NUDT16L1/SDOS. Component of cytoplasmic complexes, which also contain GIT1, ARHGEF6 and PAK1. Binds ASAP2 and PDCD10 (By similarity). Interacts with PARVA, PARVB, SORBS1 and TGFB1I1. Interacts with NEK3 and this interaction is prolactin-dependent (By similarity). Interacts with PTK2/FAK1 and PTK2B/PYK2 (By similarity). Interacts with PTK6 (By similarity). Interacts with CD36. Interacts (via cytoplasmic domain) with CEACAM1; the interaction is phosphotyrosyl-dependent (By similarity).By similarity2 Publications

GO - Molecular functioni

  • beta-catenin binding Source: RGD
  • BH4 domain binding Source: RGD
  • integrin binding Source: RGD
  • neuropilin binding Source: RGD
  • protein complex binding Source: RGD
  • protein kinase binding Source: RGD
  • ubiquitin protein ligase binding Source: RGD
  • vinculin binding Source: RGD

Protein-protein interaction databases

BioGridi262228. 4 interactors.
DIPiDIP-48423N.
IntActiQ66H76. 1 interactor.
STRINGi10116.ENSRNOP00000043928.

Structurei

3D structure databases

ProteinModelPortaliQ66H76.
SMRiQ66H76.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini353 – 403LIM zinc-binding 1PROSITE-ProRule annotationAdd BLAST51
Domaini412 – 462LIM zinc-binding 2PROSITE-ProRule annotationAdd BLAST51
Domaini471 – 521LIM zinc-binding 3PROSITE-ProRule annotationAdd BLAST51
Domaini530 – 580LIM zinc-binding 4PROSITE-ProRule annotationAdd BLAST51

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi3 – 15LD motif 1Add BLAST13
Motifi144 – 156LD motif 2Add BLAST13
Motifi245 – 257LD motif 3Add BLAST13
Motifi294 – 305LD motif 4Add BLAST12
Motifi328 – 340LD motif 5Add BLAST13

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi46 – 53Pro-rich8

Sequence similaritiesi

Belongs to the paxillin family.Curated

Keywords - Domaini

LIM domain, Repeat

Phylogenomic databases

eggNOGiKOG1703. Eukaryota.
ENOG410XRD4. LUCA.
GeneTreeiENSGT00760000118910.
HOGENOMiHOG000018764.
HOVERGENiHBG001512.
InParanoidiQ66H76.
KOiK05760.
PhylomeDBiQ66H76.

Family and domain databases

InterProiView protein in InterPro
IPR001904. Paxillin.
IPR017305. Tgfb1i1/Leupaxin/TGFB1I1.
IPR001781. Znf_LIM.
PANTHERiPTHR24216:SF11. PTHR24216:SF11. 1 hit.
PfamiView protein in Pfam
PF00412. LIM. 4 hits.
PIRSFiPIRSF037881. Leupaxin. 1 hit.
SMARTiView protein in SMART
SM00132. LIM. 4 hits.
PROSITEiView protein in PROSITE
PS00478. LIM_DOMAIN_1. 4 hits.
PS50023. LIM_DOMAIN_2. 4 hits.

Sequencei

Sequence statusi: Complete.

Q66H76-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDDLDALLAD LESTTSHISK RPVFLSEEPP YSYPTGNHTY QEIAVPPPVP
60 70 80 90 100
PPPSSEALNG TVLDPLDQWQ PSGSRYAHQQ PPSPSPIYSS STKNSSASNP
110 120 130 140 150
QDSVGSLCSR AGEEEHVYSF PNKQKSAEPS PTVMSSSLGS NLSELDRLLL
160 170 180 190 200
ELNAVQRSPS GFSAGMVSVQ ASREPLGSWG TEGRAIILSP FFQDEAESSP
210 220 230 240 250
PLPGALSPLY GVPESNNLLG GKAGPLMKEK PKRNGGRGLE DVRPSVESLL
260 270 280 290 300
DELENSVPSP VPAITVNQGE MSSPQRVTSS QQQTRISASS ATRELDELMA
310 320 330 340 350
SLSDFKFMAQ GKTGSSSPPG GLSKPGSQLD SMLGSLQSDL NKLGVATVAK
360 370 380 390 400
GVCGACKKPI AGQVVTAMGK TWHPEHFVCT HCQEEIGSRN FFERDGQPYC
410 420 430 440 450
EKDYHSLFSP RCYYCNGPIL DKVVTALDRT WHPEHFFCAQ CGAFFGPEGF
460 470 480 490 500
HEKDGKAYCR KDYFDMFAPK CGGCARAILE NYISALNTLW HPECFVCREC
510 520 530 540 550
FTPFVNGSFF EHDGQPYCEV HYHERRGSLC SGCQKPITGR CITAMAKKFH
560 570 580
PEHFVCAFCL KQLNKGTFKE QNDKPYCQSC FLKLFC
Length:586
Mass (Da):64,019
Last modified:October 11, 2004 - v1
Checksum:iD4B9477CC0B7BB7E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC081984 mRNA. Translation: AAH81984.1.
RefSeqiNP_001012147.1. NM_001012147.1.
UniGeneiRn.75.

Genome annotation databases

EnsembliENSRNOT00000047466; ENSRNOP00000043928; ENSRNOG00000001149.
GeneIDi360820.
KEGGirno:360820.

Similar proteinsi

Entry informationi

Entry nameiPAXI_RAT
AccessioniPrimary (citable) accession number: Q66H76
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: October 11, 2004
Last modified: November 22, 2017
This is version 116 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families