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Protein

Paxillin

Gene

Pxn

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).By similarity

GO - Molecular functioni

  • integrin binding Source: RGD
  • protein complex binding Source: RGD
  • protein kinase binding Source: RGD
  • zinc ion binding Source: InterPro

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-RNO-180292. GAB1 signalosome.
R-RNO-4420097. VEGFA-VEGFR2 Pathway.
R-RNO-445355. Smooth Muscle Contraction.
R-RNO-8849471. PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases.

Names & Taxonomyi

Protein namesi
Recommended name:
Paxillin
Gene namesi
Name:Pxn
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 12

Organism-specific databases

RGDi1305759. Pxn.

Subcellular locationi

  • Cytoplasmcytoskeleton By similarity
  • Cell junctionfocal adhesion By similarity
  • Cytoplasmcell cortex By similarity

  • Note: Colocalizes with integrins at the cell periphery. Colocalizes with PXN to membrane ruffles and the leading edge of migrating cells (By similarity).By similarity

GO - Cellular componenti

  • cell cortex Source: UniProtKB-SubCell
  • focal adhesion Source: RGD
  • lamellipodium Source: Ensembl
  • nucleoplasm Source: Ensembl
  • plasma membrane Source: Ensembl
  • stress fiber Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cytoplasm, Cytoskeleton

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL5613.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 586586PaxillinPRO_0000075856Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei31 – 311Phosphotyrosine; by PTK6By similarity
Modified residuei83 – 831PhosphoserineCombined sources
Modified residuei85 – 851PhosphoserineBy similarity
Modified residuei88 – 881PhosphotyrosineBy similarity
Modified residuei106 – 1061PhosphoserineBy similarity
Modified residuei118 – 1181Phosphotyrosine; by PTK6By similarity
Modified residuei119 – 1191PhosphoserineBy similarity
Modified residuei126 – 1261PhosphoserineCombined sources
Modified residuei130 – 1301PhosphoserineCombined sources
Modified residuei132 – 1321PhosphothreonineBy similarity
Modified residuei137 – 1371PhosphoserineBy similarity
Modified residuei140 – 1401PhosphoserineBy similarity
Modified residuei143 – 1431PhosphoserineCombined sources
Modified residuei210 – 2101PhosphotyrosineBy similarity
Modified residuei259 – 2591PhosphoserineCombined sources
Modified residuei273 – 2731Phosphoserine; by CDK5By similarity
Modified residuei279 – 2791PhosphoserineBy similarity
Modified residuei287 – 2871PhosphoserineCombined sources
Modified residuei290 – 2901PhosphoserineCombined sources
Modified residuei301 – 3011PhosphoserineBy similarity
Modified residuei317 – 3171PhosphoserineBy similarity
Modified residuei327 – 3271PhosphoserineCombined sources
Modified residuei335 – 3351PhosphoserineCombined sources
Modified residuei528 – 5281PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated by MAPK1/ERK2 (By similarity). Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens. Phosphorylation at Ser-273 by CDK5 reduces its interaction with PTK2/FAK1 in matrix-cell focal adhesions (MCFA) during oligodendrocytes (OLs) differentiation (By similarity). Phosphorylation at Tyr-31 and Tyr-118 by PTK6 promote the activation of RAC1 via CRK/CrKII, thereby promoting migration and invasion (By similarity). Phosphorylation at Ser-279 by SLK is required for PXN redistribution and cell motility (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ66H76.
PRIDEiQ66H76.

PTM databases

iPTMnetiQ66H76.
PhosphoSiteiQ66H76.

Expressioni

Gene expression databases

BgeeiENSRNOG00000001149.
ExpressionAtlasiQ66H76. baseline and differential.
GenevisibleiQ66H76. RN.

Interactioni

Subunit structurei

Binds to vinculin and to the SH3 domain of SRC. Interacts with GIT1 and NUDT16L1/SDOS. Component of cytoplasmic complexes, which also contain GIT1, ARHGEF6 and PAK1. Binds ASAP2 and PDCD10 (By similarity). Interacts with PARVA, PARVB, SORBS1 and TGFB1I1. Interacts with NEK3 and this interaction is prolactin-dependent (By similarity). Interacts with PTK2/FAK1 and PTK2B/PYK2 (By similarity). Interacts with PTK6 (By similarity). Interacts with CD36.By similarity2 Publications

GO - Molecular functioni

  • integrin binding Source: RGD
  • protein complex binding Source: RGD
  • protein kinase binding Source: RGD

Protein-protein interaction databases

BioGridi262228. 4 interactions.
DIPiDIP-48423N.
STRINGi10116.ENSRNOP00000043928.

Structurei

3D structure databases

ProteinModelPortaliQ66H76.
SMRiQ66H76. Positions 461-528.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini353 – 40351LIM zinc-binding 1PROSITE-ProRule annotationAdd
BLAST
Domaini412 – 46251LIM zinc-binding 2PROSITE-ProRule annotationAdd
BLAST
Domaini471 – 52151LIM zinc-binding 3PROSITE-ProRule annotationAdd
BLAST
Domaini530 – 58051LIM zinc-binding 4PROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi3 – 1513LD motif 1Add
BLAST
Motifi144 – 15613LD motif 2Add
BLAST
Motifi245 – 25713LD motif 3Add
BLAST
Motifi294 – 30512LD motif 4Add
BLAST
Motifi328 – 34013LD motif 5Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi46 – 538Pro-rich

Sequence similaritiesi

Belongs to the paxillin family.Curated
Contains 4 LIM zinc-binding domains.PROSITE-ProRule annotation

Keywords - Domaini

LIM domain, Repeat

Phylogenomic databases

eggNOGiKOG1703. Eukaryota.
ENOG410XRD4. LUCA.
GeneTreeiENSGT00760000118910.
HOGENOMiHOG000018764.
HOVERGENiHBG001512.
InParanoidiQ66H76.
KOiK05760.
PhylomeDBiQ66H76.

Family and domain databases

Gene3Di2.10.110.10. 4 hits.
InterProiIPR001904. Paxillin.
IPR001781. Znf_LIM.
[Graphical view]
PANTHERiPTHR24216:SF11. PTHR24216:SF11. 2 hits.
PfamiPF00412. LIM. 4 hits.
[Graphical view]
SMARTiSM00132. LIM. 4 hits.
[Graphical view]
PROSITEiPS00478. LIM_DOMAIN_1. 4 hits.
PS50023. LIM_DOMAIN_2. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q66H76-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDDLDALLAD LESTTSHISK RPVFLSEEPP YSYPTGNHTY QEIAVPPPVP
60 70 80 90 100
PPPSSEALNG TVLDPLDQWQ PSGSRYAHQQ PPSPSPIYSS STKNSSASNP
110 120 130 140 150
QDSVGSLCSR AGEEEHVYSF PNKQKSAEPS PTVMSSSLGS NLSELDRLLL
160 170 180 190 200
ELNAVQRSPS GFSAGMVSVQ ASREPLGSWG TEGRAIILSP FFQDEAESSP
210 220 230 240 250
PLPGALSPLY GVPESNNLLG GKAGPLMKEK PKRNGGRGLE DVRPSVESLL
260 270 280 290 300
DELENSVPSP VPAITVNQGE MSSPQRVTSS QQQTRISASS ATRELDELMA
310 320 330 340 350
SLSDFKFMAQ GKTGSSSPPG GLSKPGSQLD SMLGSLQSDL NKLGVATVAK
360 370 380 390 400
GVCGACKKPI AGQVVTAMGK TWHPEHFVCT HCQEEIGSRN FFERDGQPYC
410 420 430 440 450
EKDYHSLFSP RCYYCNGPIL DKVVTALDRT WHPEHFFCAQ CGAFFGPEGF
460 470 480 490 500
HEKDGKAYCR KDYFDMFAPK CGGCARAILE NYISALNTLW HPECFVCREC
510 520 530 540 550
FTPFVNGSFF EHDGQPYCEV HYHERRGSLC SGCQKPITGR CITAMAKKFH
560 570 580
PEHFVCAFCL KQLNKGTFKE QNDKPYCQSC FLKLFC
Length:586
Mass (Da):64,019
Last modified:October 11, 2004 - v1
Checksum:iD4B9477CC0B7BB7E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC081984 mRNA. Translation: AAH81984.1.
RefSeqiNP_001012147.1. NM_001012147.1.
UniGeneiRn.75.

Genome annotation databases

EnsembliENSRNOT00000047466; ENSRNOP00000043928; ENSRNOG00000001149.
GeneIDi360820.
KEGGirno:360820.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC081984 mRNA. Translation: AAH81984.1.
RefSeqiNP_001012147.1. NM_001012147.1.
UniGeneiRn.75.

3D structure databases

ProteinModelPortaliQ66H76.
SMRiQ66H76. Positions 461-528.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi262228. 4 interactions.
DIPiDIP-48423N.
STRINGi10116.ENSRNOP00000043928.

Chemistry

ChEMBLiCHEMBL5613.

PTM databases

iPTMnetiQ66H76.
PhosphoSiteiQ66H76.

Proteomic databases

PaxDbiQ66H76.
PRIDEiQ66H76.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000047466; ENSRNOP00000043928; ENSRNOG00000001149.
GeneIDi360820.
KEGGirno:360820.

Organism-specific databases

CTDi5829.
RGDi1305759. Pxn.

Phylogenomic databases

eggNOGiKOG1703. Eukaryota.
ENOG410XRD4. LUCA.
GeneTreeiENSGT00760000118910.
HOGENOMiHOG000018764.
HOVERGENiHBG001512.
InParanoidiQ66H76.
KOiK05760.
PhylomeDBiQ66H76.

Enzyme and pathway databases

ReactomeiR-RNO-180292. GAB1 signalosome.
R-RNO-4420097. VEGFA-VEGFR2 Pathway.
R-RNO-445355. Smooth Muscle Contraction.
R-RNO-8849471. PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases.

Miscellaneous databases

PROiQ66H76.

Gene expression databases

BgeeiENSRNOG00000001149.
ExpressionAtlasiQ66H76. baseline and differential.
GenevisibleiQ66H76. RN.

Family and domain databases

Gene3Di2.10.110.10. 4 hits.
InterProiIPR001904. Paxillin.
IPR001781. Znf_LIM.
[Graphical view]
PANTHERiPTHR24216:SF11. PTHR24216:SF11. 2 hits.
PfamiPF00412. LIM. 4 hits.
[Graphical view]
SMARTiSM00132. LIM. 4 hits.
[Graphical view]
PROSITEiPS00478. LIM_DOMAIN_1. 4 hits.
PS50023. LIM_DOMAIN_2. 4 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPAXI_RAT
AccessioniPrimary (citable) accession number: Q66H76
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: October 11, 2004
Last modified: September 7, 2016
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.