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Protein

Interleukin-33

Gene

Il33

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells. Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines. Also involved in activation of mast cells, basophils, eosinophils and natural killer cells. Acts as a chemoattractant for Th2 cells, and may function as an "alarmin", that amplifies immune responses during tissue injury (By similarity).By similarity
In quiescent endothelia the uncleaved form is constitutively and abundantly expressed, and acts as a chromatin-associated nuclear factor with transcriptional repressor properties, it may sequester nuclear NF-kappaB/RELA, lowering expression of its targets. This form is rapidely lost upon angiogenic or proinflammatory activation (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Cytokine

Keywords - Biological processi

Transcription

Names & Taxonomyi

Protein namesi
Recommended name:
Interleukin-33
Short name:
IL-33
Gene namesi
Name:Il33
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi1311155. Il33.

Subcellular locationi

  • Nucleus By similarity
  • Chromosome By similarity
  • Cytoplasmic vesiclesecretory vesicle By similarity
  • Secreted By similarity

  • Note: Associates with heterochromatin and mitotic chromosomes.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Cytoplasmic vesicle, Nucleus, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 264264Interleukin-33PRO_0000096793Add
BLAST
Propeptidei1 – 101101By similarityPRO_0000430091Add
BLAST

Post-translational modificationi

The full length protein can be released from cells and is able to signal via the IL1RL1/ST2 receptor. However, proteolytic processing by CSTG/cathepsin G and ELANE/neutrophil elastase produces C-terminal peptides that are more active than the unprocessed full length protein. May also be proteolytically processed by calpains. Proteolytic cleavage mediated by apoptotic caspases including CASP3 and CASP7 results in IL33 inactivation. In vitro proteolytic cleavage by CASP1 was reported but could not be confirmed in vivo suggesting that IL33 is probably not a direct substrate for that caspase (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei101 – 1022Cleavage; by CTSG and ELANEBy similarity
Sitei108 – 1092Cleavage; by ELANEBy similarity

Proteomic databases

PRIDEiQ66H70.

PTM databases

iPTMnetiQ66H70.
PhosphoSiteiQ66H70.

Interactioni

Subunit structurei

Forms a 1:1:1 heterotrimeric complex with its primary high-affinity receptor IL1RL1 and the coreceptor IL1RAP.By similarity

GO - Molecular functioni

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 6565Homeodomain-like HTH domainBy similarityAdd
BLAST
Regioni66 – 10843Interaction with RELABy similarityAdd
BLAST

Domaini

The homeodomain-like HTH domain mediates nuclear localization and heterochromatin association.By similarity

Sequence similaritiesi

Belongs to the IL-1 family. Highly divergent.Curated

Phylogenomic databases

HOGENOMiHOG000070215.
HOVERGENiHBG081791.
InParanoidiQ66H70.
KOiK12967.
OrthoDBiEOG7TQV1R.
PhylomeDBiQ66H70.
TreeFamiTF338120.

Family and domain databases

InterProiIPR026145. IL-33.
[Graphical view]
PANTHERiPTHR21114. PTHR21114. 1 hit.
PfamiPF15095. IL33. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q66H70-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRPRMKYSNS KISPAKCDST SGRALVPPCK ITRSQQKTKD ICHVYCMRLR
60 70 80 90 100
SGLTIRKETC YFGKEPAKRY SLKSGSKHEG RLSTCLPDSR KRSLLGSIQA
110 120 130 140 150
FAASVDTLSI QGTSLLTESC ALSTYNDQSV SFVLENGCYV INVEDCGKNQ
160 170 180 190 200
EKDKVLLRYY ESSFPAQSGD GVDGKKLMVN MSPIKDTDIW LNANDKDYSV
210 220 230 240 250
ELQKGDVSPP DQAFFVLHKK SSDFVSFECK NLPGTYIGVK DNQLALVEEN
260
DESCNNIMFK LSKM
Length:264
Mass (Da):29,486
Last modified:October 11, 2004 - v1
Checksum:i91452ADC579B7BCC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC081993 mRNA. Translation: AAH81993.1.
RefSeqiNP_001014188.1. NM_001014166.1.
UniGeneiRn.106849.

Genome annotation databases

GeneIDi361749.
KEGGirno:361749.
UCSCiRGD:1311155. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC081993 mRNA. Translation: AAH81993.1.
RefSeqiNP_001014188.1. NM_001014166.1.
UniGeneiRn.106849.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

PTM databases

iPTMnetiQ66H70.
PhosphoSiteiQ66H70.

Proteomic databases

PRIDEiQ66H70.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi361749.
KEGGirno:361749.
UCSCiRGD:1311155. rat.

Organism-specific databases

CTDi90865.
RGDi1311155. Il33.

Phylogenomic databases

HOGENOMiHOG000070215.
HOVERGENiHBG081791.
InParanoidiQ66H70.
KOiK12967.
OrthoDBiEOG7TQV1R.
PhylomeDBiQ66H70.
TreeFamiTF338120.

Miscellaneous databases

PROiQ66H70.

Family and domain databases

InterProiIPR026145. IL-33.
[Graphical view]
PANTHERiPTHR21114. PTHR21114. 1 hit.
PfamiPF15095. IL33. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.

Entry informationi

Entry nameiIL33_RAT
AccessioniPrimary (citable) accession number: Q66H70
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: October 11, 2004
Last modified: July 6, 2016
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.