Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q66H62

- CYLD_RAT

UniProt

Q66H62 - CYLD_RAT

Protein

Ubiquitin carboxyl-terminal hydrolase CYLD

Gene

Cyld

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 83 (01 Oct 2014)
      Sequence version 1 (11 Oct 2004)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Protease that specifically cleaves 'Lys-63'-linked polyubiquitin chains. Has endodeubiquitinase activity. Plays an important role in the regulation of pathways leading to NF-kappa-B activation. Contributes to the regulation of cell survival, proliferation and differentiation via its effects on NF-kappa-B activation. Negative regulator of Wnt signaling. Inhibits HDAC6 and thereby promotes acetylation of alpha-tubulin and stabilization of microtubules. Plays a role in the regulation of microtubule dynamics, and thereby contributes to the regulation of cell proliferation, cell polarization, cell migration, and angiogenesis. Required for normal cell cycle progress and normal cytokinesis. Inhibits nuclear translocation of NF-kappa-B By similarity. Plays a role in the regulation of inflammation and the innate immune response, via its effects on NF-kappa-B activation. Dispensable for the maturation of intrathymic natural killer cells, but required for the continued survival of immature natural killer cells. Negatively regulates TNFRSF11A signaling and osteoclastogenesis By similarity.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei598 – 5981NucleophilePROSITE-ProRule annotation
    Metal bindingi785 – 7851Zinc 1By similarity
    Metal bindingi788 – 7881Zinc 1By similarity
    Metal bindingi796 – 7961Zinc 2By similarity
    Metal bindingi799 – 7991Zinc 2By similarity
    Metal bindingi814 – 8141Zinc 1By similarity
    Metal bindingi817 – 8171Zinc 1By similarity
    Metal bindingi822 – 8221Zinc 2By similarity
    Metal bindingi830 – 8301Zinc 2By similarity
    Active sitei868 – 8681Proton acceptorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. Lys63-specific deubiquitinase activity Source: RefGenome
    2. ubiquitin-specific protease activity Source: UniProtKB
    3. zinc ion binding Source: UniProtKB

    GO - Biological processi

    1. necroptotic process Source: RefGenome
    2. negative regulation of canonical Wnt signaling pathway Source: UniProtKB
    3. negative regulation of NF-kappaB import into nucleus Source: UniProtKB
    4. negative regulation of NF-kappaB transcription factor activity Source: UniProtKB
    5. positive regulation of extrinsic apoptotic signaling pathway Source: RefGenome
    6. protein K63-linked deubiquitination Source: UniProtKB
    7. regulation of intrinsic apoptotic signaling pathway Source: RefGenome
    8. regulation of mitotic cell cycle Source: RefGenome
    9. ubiquitin-dependent protein catabolic process Source: InterPro
    10. Wnt signaling pathway Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Ubl conjugation pathway, Wnt signaling pathway

    Keywords - Ligandi

    Metal-binding, Zinc

    Protein family/group databases

    MEROPSiC67.001.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase CYLD (EC:3.4.19.12)
    Alternative name(s):
    Deubiquitinating enzyme CYLD
    Ubiquitin thioesterase CYLD
    Ubiquitin-specific-processing protease CYLD
    Gene namesi
    Name:Cyld
    Synonyms:Cyld1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi1308346. Cyld.

    Subcellular locationi

    Cytoplasm By similarity. Cytoplasmperinuclear region By similarity. Cytoplasmcytoskeleton By similarity. Cell membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
    Note: Detected at the microtubule cytoskeleton during interphase. Detected at the midbody during telophase By similarity.By similarity

    GO - Cellular componenti

    1. cytosol Source: UniProtKB
    2. microtubule Source: UniProtKB-KW
    3. perinuclear region of cytoplasm Source: UniProtKB-SubCell
    4. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Cytoskeleton, Membrane, Microtubule

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 953953Ubiquitin carboxyl-terminal hydrolase CYLDPRO_0000326149Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei415 – 4151PhosphoserineBy similarity

    Post-translational modificationi

    Phosphorylated on several serine residues by IKKA and/or IKKB in response to immune stimuli. Phosphorylation requires IKBKG. Phosphorylation abolishes TRAF2 deubiquitination, interferes with the activation of Jun kinases, and strongly reduces CD40-dependent gene activation by NF-kappa-B By similarity.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ66H62.
    PRIDEiQ66H62.

    Expressioni

    Gene expression databases

    GenevestigatoriQ66H62.

    Interactioni

    Subunit structurei

    Interacts (via CAP-Gly domain) with IKBKG/NEMO (via proline-rich C-terminal region). Interacts with TRAF2 and TRIP. Interacts with PLK1, DVL1, DVL3, MAVS, TBK1, IKKE and DDX58. Interacts (via CAP-Gly domain) with microtubules. Interacts with HDAC6 and BCL3. Interacts with SQSTM1 and MAP3K7. Identified in a complex with TRAF6 and SQSTM1 By similarity.By similarity

    Protein-protein interaction databases

    BioGridi260280. 1 interaction.
    STRINGi10116.ENSRNOP00000057517.

    Structurei

    3D structure databases

    ProteinModelPortaliQ66H62.
    SMRiQ66H62. Positions 125-206, 228-306, 462-547, 580-953.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini153 – 19846CAP-Gly 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini253 – 28634CAP-Gly 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini489 – 53244CAP-Gly 3PROSITE-ProRule annotationAdd
    BLAST
    Domaini589 – 947359USPAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni106 – 590485Interaction with TRIPBy similarityAdd
    BLAST
    Regioni391 – 46676Interaction with TRAF2By similarityAdd
    BLAST
    Regioni467 – 681215Interaction with IKBKG/NEMOBy similarityAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase C19 family.Curated
    Contains 3 CAP-Gly domains.PROSITE-ProRule annotation
    Contains 1 USP domain.Curated

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG313578.
    HOGENOMiHOG000006796.
    HOVERGENiHBG051281.
    KOiK08601.
    PhylomeDBiQ66H62.

    Family and domain databases

    Gene3Di2.30.30.190. 3 hits.
    InterProiIPR000938. CAP-Gly_domain.
    IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    [Graphical view]
    PfamiPF01302. CAP_GLY. 3 hits.
    PF00443. UCH. 1 hit.
    [Graphical view]
    SMARTiSM01052. CAP_GLY. 3 hits.
    [Graphical view]
    SUPFAMiSSF74924. SSF74924. 3 hits.
    PROSITEiPS00845. CAP_GLY_1. 1 hit.
    PS50245. CAP_GLY_2. 2 hits.
    PS00972. USP_1. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q66H62-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSGLWNQEK VTSPYWEERL FYLLLQECSV TDKQTQKLLR VPKGSIGQYI    50
    QDRSVGHSRV PSAKGKKNQI GLKILEQPHA VLFVDEKDVV EINEKFTELL 100
    LAITNCEERL SLFRNRIRLS KGLQVDVGSP VRVQLRSGEE KFPGVVRFRG 150
    PLLAERTVSG IFFGVELLEE GRGQGFTDGV YQGKQLFQCD EDCGVFVALD 200
    KLELIEDDDN GLESDFAGPG DTVQVEPPPL EINSRVSLKV GESTESGTVI 250
    FCDVLPGKES LGYFVGVDMD NPIGNWDGRF DGVQLCSFAS VESTVLLHIN 300
    DIIPDSVTQE RRPPKLAFMS RGVGDKGSSS HNKPKVTGST SDPGSRNRSE 350
    LFYTLNGSSV DSQQQSKSKN PWYIDEVAED PAKSLTEMSS DFGHSSPPPQ 400
    PPSMNSLSSE NRFHSLPFSL TKMPNTNGSM AHSPLSLSVQ SVMGELNSTP 450
    VQESPPMPSS SGNAHGLEVG SLAEVKENPP FYGVIRWIGQ PPGLSDVLAG 500
    LELEDECAGC TDGTFRGTRY FTCALKKALF VKLKSCRPDS RFASLQPVSN 550
    QIERCNSLAF GGYLSEVVEE NTPPKMEKEG LEIMIGKKKG IQGHYNSCYL 600
    DSTLFCLFAF SSALDTVLLR PKEKNDVEYY SETQELLRTE IVNPLRIYGY 650
    VCATKIMKLR KILEKVEAAS GFTSEEKDPE EFLNILFHDI LRVEPLLKIR 700
    SAGQKVQDCN FYQIFMEKNE KVGVPTIQQL LEWSFINSNL KFAEAPSCLI 750
    IQMPRFGKDF KLFKKIFPSL ELNITDLLED TPRQCRICGG LAMYECRECY 800
    DDPDISAGKI KQFCKTCSTQ VHLHPRRLNH TYHPVSLPKD LPDWDWRHGC 850
    IPCQKMELFA VLCIETSHYV AFVKYGKDDS AWLFFDSMAD RDGGQNGFNI 900
    PQVTPCPEVG EYLKMSLEDL HSLDSRRIQG CARRLLCDAY MCMYQSPTMS 950
    LYK 953
    Length:953
    Mass (Da):106,713
    Last modified:October 11, 2004 - v1
    Checksum:i573B59E9BD795252
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC082001 mRNA. Translation: AAH82001.1.
    RefSeqiNP_001017380.1. NM_001017380.1.
    UniGeneiRn.128760.
    Rn.168938.

    Genome annotation databases

    GeneIDi312937.
    KEGGirno:312937.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC082001 mRNA. Translation: AAH82001.1 .
    RefSeqi NP_001017380.1. NM_001017380.1.
    UniGenei Rn.128760.
    Rn.168938.

    3D structure databases

    ProteinModelPortali Q66H62.
    SMRi Q66H62. Positions 125-206, 228-306, 462-547, 580-953.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 260280. 1 interaction.
    STRINGi 10116.ENSRNOP00000057517.

    Protein family/group databases

    MEROPSi C67.001.

    Proteomic databases

    PaxDbi Q66H62.
    PRIDEi Q66H62.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 312937.
    KEGGi rno:312937.

    Organism-specific databases

    CTDi 1540.
    RGDi 1308346. Cyld.

    Phylogenomic databases

    eggNOGi NOG313578.
    HOGENOMi HOG000006796.
    HOVERGENi HBG051281.
    KOi K08601.
    PhylomeDBi Q66H62.

    Miscellaneous databases

    NextBioi 665399.

    Gene expression databases

    Genevestigatori Q66H62.

    Family and domain databases

    Gene3Di 2.30.30.190. 3 hits.
    InterProi IPR000938. CAP-Gly_domain.
    IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    [Graphical view ]
    Pfami PF01302. CAP_GLY. 3 hits.
    PF00443. UCH. 1 hit.
    [Graphical view ]
    SMARTi SM01052. CAP_GLY. 3 hits.
    [Graphical view ]
    SUPFAMi SSF74924. SSF74924. 3 hits.
    PROSITEi PS00845. CAP_GLY_1. 1 hit.
    PS50245. CAP_GLY_2. 2 hits.
    PS00972. USP_1. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Testis.

    Entry informationi

    Entry nameiCYLD_RAT
    AccessioniPrimary (citable) accession number: Q66H62
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 18, 2008
    Last sequence update: October 11, 2004
    Last modified: October 1, 2014
    This is version 83 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3