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Q66H59

- NPL_RAT

UniProt

Q66H59 - NPL_RAT

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Protein

N-acetylneuraminate lyase

Gene
Npl
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalyzes the cleavage of N-acetylneuraminic acid (sialic acid) to form pyruvate and N-acetylmannosamine via a Schiff base intermediate. It prevents sialic acids from being recycled and returning to the cell surface. Involved in the N-glycolylneuraminic acid (Neu5Gc) degradation pathway By similarity.

Catalytic activityi

N-acetylneuraminate = N-acetyl-D-mannosamine + pyruvate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei143 – 1431Involved in proton transfer during cleavage By similarity
Active sitei173 – 1731Schiff-base intermediate with substrate By similarity

GO - Molecular functioni

  1. N-acetylneuraminate lyase activity Source: UniProtKB

GO - Biological processi

  1. carbohydrate metabolic process Source: UniProtKB-KW
  2. N-acetylneuraminate catabolic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

Schiff base

Enzyme and pathway databases

ReactomeiREACT_213723. Sialic acid metabolism.
UniPathwayiUPA00629.

Names & Taxonomyi

Protein namesi
Recommended name:
N-acetylneuraminate lyase (EC:4.1.3.3)
Short name:
NALase
Alternative name(s):
N-acetylneuraminate pyruvate-lyase
N-acetylneuraminic acid aldolase
Sialate lyase
Sialate-pyruvate lyase
Sialic acid aldolase
Sialic acid lyase
Gene namesi
Name:Npl
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 13

Organism-specific databases

RGDi1549702. Npl.

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 320320N-acetylneuraminate lyasePRO_0000273356Add
BLAST

Proteomic databases

PaxDbiQ66H59.
PRIDEiQ66H59.

Expressioni

Gene expression databases

GenevestigatoriQ66H59.

Interactioni

Subunit structurei

Homotetramer By similarity.

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000003713.

Structurei

3D structure databases

ProteinModelPortaliQ66H59.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni51 – 522Substrate binding By similarity

Sequence similaritiesi

Belongs to the DapA family. NanA subfamily.

Phylogenomic databases

eggNOGiCOG0329.
GeneTreeiENSGT00530000063604.
HOGENOMiHOG000218206.
HOVERGENiHBG082055.
InParanoidiQ66H59.
KOiK01639.
OMAiLKPWNKD.
OrthoDBiEOG7NKKKP.
PhylomeDBiQ66H59.
TreeFamiTF353639.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR002220. DapA-like.
[Graphical view]
PANTHERiPTHR12128. PTHR12128. 1 hit.
PfamiPF00701. DHDPS. 1 hit.
[Graphical view]
PIRSFiPIRSF001365. DHDPS. 1 hit.
PRINTSiPR00146. DHPICSNTHASE.

Sequencei

Sequence statusi: Complete.

Q66H59-1 [UniParc]FASTAAdd to Basket

« Hide

MAFPKKKLQG LVAATITPMT ENGEINFPVI GQYVDYLVKE QGVKNIFVNG    50
TTGEGLSLSI SERRQVAEEW VRQGKNKLDQ VVIHVGALNL KESQELAQHA 100
AEIGADGIAV IAPFFFKSQN KDALISFLRE VAAAAPALPF YYYHIPSLTG 150
VKIRAEELLD GIQDKIPSFQ GLKFSDTDLL DFGQCVDQNH QRQFALLFGV 200
DEQLLSALVL GATGAVGSTY NYLGKKTNQM LEAFEQKDLA SALSYQFRIQ 250
RFINYVIKLG FGVSQTKAIM TLVSGIPMGP PRLPLQKATQ EFTANAEAKL 300
KSLNFLSFPG LKDGNMEACS 320
Length:320
Mass (Da):35,115
Last modified:October 11, 2004 - v1
Checksum:i3674F314288D8B89
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC082004 mRNA. Translation: AAH82004.1.
RefSeqiNP_001014006.1. NM_001013984.1.
UniGeneiRn.114492.

Genome annotation databases

EnsembliENSRNOT00000003713; ENSRNOP00000003713; ENSRNOG00000002775.
GeneIDi304860.
KEGGirno:304860.
UCSCiRGD:1549702. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC082004 mRNA. Translation: AAH82004.1 .
RefSeqi NP_001014006.1. NM_001013984.1.
UniGenei Rn.114492.

3D structure databases

ProteinModelPortali Q66H59.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000003713.

Proteomic databases

PaxDbi Q66H59.
PRIDEi Q66H59.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000003713 ; ENSRNOP00000003713 ; ENSRNOG00000002775 .
GeneIDi 304860.
KEGGi rno:304860.
UCSCi RGD:1549702. rat.

Organism-specific databases

CTDi 80896.
RGDi 1549702. Npl.

Phylogenomic databases

eggNOGi COG0329.
GeneTreei ENSGT00530000063604.
HOGENOMi HOG000218206.
HOVERGENi HBG082055.
InParanoidi Q66H59.
KOi K01639.
OMAi LKPWNKD.
OrthoDBi EOG7NKKKP.
PhylomeDBi Q66H59.
TreeFami TF353639.

Enzyme and pathway databases

UniPathwayi UPA00629 .
Reactomei REACT_213723. Sialic acid metabolism.

Miscellaneous databases

NextBioi 653735.
PROi Q66H59.

Gene expression databases

Genevestigatori Q66H59.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
InterProi IPR013785. Aldolase_TIM.
IPR002220. DapA-like.
[Graphical view ]
PANTHERi PTHR12128. PTHR12128. 1 hit.
Pfami PF00701. DHDPS. 1 hit.
[Graphical view ]
PIRSFi PIRSF001365. DHDPS. 1 hit.
PRINTSi PR00146. DHPICSNTHASE.
ProtoNeti Search...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.

Entry informationi

Entry nameiNPL_RAT
AccessioniPrimary (citable) accession number: Q66H59
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: October 11, 2004
Last modified: September 3, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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