Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q66H59

- NPL_RAT

UniProt

Q66H59 - NPL_RAT

Protein

N-acetylneuraminate lyase

Gene

Npl

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 83 (01 Oct 2014)
      Sequence version 1 (11 Oct 2004)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the cleavage of N-acetylneuraminic acid (sialic acid) to form pyruvate and N-acetylmannosamine via a Schiff base intermediate. It prevents sialic acids from being recycled and returning to the cell surface. Involved in the N-glycolylneuraminic acid (Neu5Gc) degradation pathway By similarity.By similarity

    Catalytic activityi

    N-acetylneuraminate = N-acetyl-D-mannosamine + pyruvate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei143 – 1431Involved in proton transfer during cleavageBy similarity
    Active sitei173 – 1731Schiff-base intermediate with substrateBy similarity

    GO - Molecular functioni

    1. N-acetylneuraminate lyase activity Source: UniProtKB

    GO - Biological processi

    1. carbohydrate metabolic process Source: UniProtKB-KW
    2. N-acetylneuraminate catabolic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Carbohydrate metabolism

    Keywords - Ligandi

    Schiff base

    Enzyme and pathway databases

    ReactomeiREACT_213723. Sialic acid metabolism.
    UniPathwayiUPA00629.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    N-acetylneuraminate lyase (EC:4.1.3.3)
    Short name:
    NALase
    Alternative name(s):
    N-acetylneuraminate pyruvate-lyase
    N-acetylneuraminic acid aldolase
    Sialate lyase
    Sialate-pyruvate lyase
    Sialic acid aldolase
    Sialic acid lyase
    Gene namesi
    Name:Npl
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 13

    Organism-specific databases

    RGDi1549702. Npl.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 320320N-acetylneuraminate lyasePRO_0000273356Add
    BLAST

    Proteomic databases

    PaxDbiQ66H59.
    PRIDEiQ66H59.

    Expressioni

    Gene expression databases

    GenevestigatoriQ66H59.

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000003713.

    Structurei

    3D structure databases

    ProteinModelPortaliQ66H59.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni51 – 522Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the DapA family. NanA subfamily.Curated

    Phylogenomic databases

    eggNOGiCOG0329.
    GeneTreeiENSGT00530000063604.
    HOGENOMiHOG000218206.
    HOVERGENiHBG082055.
    InParanoidiQ66H59.
    KOiK01639.
    OMAiLKPWNKD.
    OrthoDBiEOG7NKKKP.
    PhylomeDBiQ66H59.
    TreeFamiTF353639.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    InterProiIPR013785. Aldolase_TIM.
    IPR002220. DapA-like.
    [Graphical view]
    PANTHERiPTHR12128. PTHR12128. 1 hit.
    PfamiPF00701. DHDPS. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001365. DHDPS. 1 hit.
    PRINTSiPR00146. DHPICSNTHASE.

    Sequencei

    Sequence statusi: Complete.

    Q66H59-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAFPKKKLQG LVAATITPMT ENGEINFPVI GQYVDYLVKE QGVKNIFVNG    50
    TTGEGLSLSI SERRQVAEEW VRQGKNKLDQ VVIHVGALNL KESQELAQHA 100
    AEIGADGIAV IAPFFFKSQN KDALISFLRE VAAAAPALPF YYYHIPSLTG 150
    VKIRAEELLD GIQDKIPSFQ GLKFSDTDLL DFGQCVDQNH QRQFALLFGV 200
    DEQLLSALVL GATGAVGSTY NYLGKKTNQM LEAFEQKDLA SALSYQFRIQ 250
    RFINYVIKLG FGVSQTKAIM TLVSGIPMGP PRLPLQKATQ EFTANAEAKL 300
    KSLNFLSFPG LKDGNMEACS 320
    Length:320
    Mass (Da):35,115
    Last modified:October 11, 2004 - v1
    Checksum:i3674F314288D8B89
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC082004 mRNA. Translation: AAH82004.1.
    RefSeqiNP_001014006.1. NM_001013984.1.
    UniGeneiRn.114492.

    Genome annotation databases

    EnsembliENSRNOT00000003713; ENSRNOP00000003713; ENSRNOG00000002775.
    GeneIDi304860.
    KEGGirno:304860.
    UCSCiRGD:1549702. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC082004 mRNA. Translation: AAH82004.1 .
    RefSeqi NP_001014006.1. NM_001013984.1.
    UniGenei Rn.114492.

    3D structure databases

    ProteinModelPortali Q66H59.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000003713.

    Proteomic databases

    PaxDbi Q66H59.
    PRIDEi Q66H59.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000003713 ; ENSRNOP00000003713 ; ENSRNOG00000002775 .
    GeneIDi 304860.
    KEGGi rno:304860.
    UCSCi RGD:1549702. rat.

    Organism-specific databases

    CTDi 80896.
    RGDi 1549702. Npl.

    Phylogenomic databases

    eggNOGi COG0329.
    GeneTreei ENSGT00530000063604.
    HOGENOMi HOG000218206.
    HOVERGENi HBG082055.
    InParanoidi Q66H59.
    KOi K01639.
    OMAi LKPWNKD.
    OrthoDBi EOG7NKKKP.
    PhylomeDBi Q66H59.
    TreeFami TF353639.

    Enzyme and pathway databases

    UniPathwayi UPA00629 .
    Reactomei REACT_213723. Sialic acid metabolism.

    Miscellaneous databases

    NextBioi 653735.
    PROi Q66H59.

    Gene expression databases

    Genevestigatori Q66H59.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    InterProi IPR013785. Aldolase_TIM.
    IPR002220. DapA-like.
    [Graphical view ]
    PANTHERi PTHR12128. PTHR12128. 1 hit.
    Pfami PF00701. DHDPS. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001365. DHDPS. 1 hit.
    PRINTSi PR00146. DHPICSNTHASE.
    ProtoNeti Search...

    Publicationsi

    1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Testis.

    Entry informationi

    Entry nameiNPL_RAT
    AccessioniPrimary (citable) accession number: Q66H59
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 23, 2007
    Last sequence update: October 11, 2004
    Last modified: October 1, 2014
    This is version 83 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3