Q66H59 (NPL_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
N-acetylneuraminate lyase
Short name=NALase
EC=4.1.3.3

Alternative name(s):
N-acetylneuraminate pyruvate-lyase
N-acetylneuraminic acid aldolase
Sialate lyase
Sialate-pyruvate lyase
Sialic acid aldolase
Sialic acid lyase

Gene names

Name:

Npl

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	320 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the cleavage of N-acetylneuraminic acid (sialic acid) to form pyruvate and N-acetylmannosamine via a Schiff base intermediate. It prevents sialic acids from being recycled and returning to the cell surface. Involved in the N-glycolylneuraminic acid (Neu5Gc) degradation pathway By similarity.
Catalytic activity	N-acetylneuraminate = N-acetyl-D-mannosamine + pyruvate.
Pathway	Amino-sugar metabolism; N-acetylneuraminate degradation.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the DapA family. NanA subfamily.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Cellular component	Cytoplasm
Ligand	Schiff base
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	N-acetylneuraminate catabolic process Inferred from electronic annotation. Source: UniProtKB-UniPathway carbohydrate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	N-acetylneuraminate lyase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 320	320	N-acetylneuraminate lyase		PRO_0000273356
Regions
	Region	51 – 52	2	Substrate binding By similarity
Sites
	Active site	173	1	Schiff-base intermediate with substrate By similarity
	Site	143	1	Involved in proton transfer during cleavage By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q66H59 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 3674F314288D8B89	FASTA	320	35,115
	10 20 30 40 50 60 MAFPKKKLQG LVAATITPMT ENGEINFPVI GQYVDYLVKE QGVKNIFVNG TTGEGLSLSI 70 80 90 100 110 120 SERRQVAEEW VRQGKNKLDQ VVIHVGALNL KESQELAQHA AEIGADGIAV IAPFFFKSQN 130 140 150 160 170 180 KDALISFLRE VAAAAPALPF YYYHIPSLTG VKIRAEELLD GIQDKIPSFQ GLKFSDTDLL 190 200 210 220 230 240 DFGQCVDQNH QRQFALLFGV DEQLLSALVL GATGAVGSTY NYLGKKTNQM LEAFEQKDLA 250 260 270 280 290 300 SALSYQFRIQ RFINYVIKLG FGVSQTKAIM TLVSGIPMGP PRLPLQKATQ EFTANAEAKL 310 320 KSLNFLSFPG LKDGNMEACS

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC082004 mRNA. Translation: AAH82004.1.
IPI	IPI00471794.
RefSeq	NP_001014006.1. NM_001013984.1.
UniGene	Rn.114492.
3D structure databases
HSSP	HSSP built from PDB template 1F6K based on UniProtKB P44539.
ProteinModelPortal	Q66H59.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000003713.
Proteomic databases
PaxDb	Q66H59.
PRIDE	Q66H59.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000003713; ENSRNOP00000003713; ENSRNOG00000002775.
GeneID	304860.
KEGG	rno:304860.
UCSC	RGD:1549702. rat.
Organism-specific databases
CTD	80896.
RGD	1549702. Npl.
Phylogenomic databases
eggNOG	COG0329.
GeneTree	ENSGT00530000063604.
HOGENOM	HOG000218206.
HOVERGEN	HBG082055.
InParanoid	Q66H59.
KO	K01639.
OMA	CVDQNHQ.
OrthoDB	EOG48PMM2.
Enzyme and pathway databases
UniPathway	UPA00629.
Gene expression databases
Genevestigator	Q66H59.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
InterPro	IPR013785. Aldolase_TIM. IPR002220. Dihydrodipicolinate_synth-like. [Graphical view]
PANTHER	PTHR12128. PTHR12128. 1 hit.
Pfam	PF00701. DHDPS. 1 hit. [Graphical view]
PIRSF	PIRSF001365. DHDPS. 1 hit.
PRINTS	PR00146. DHPICSNTHASE.
PROSITE	PS00665. DHDPS_1. False negative. PS00666. DHDPS_2. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	653735.

Entry information

Entry name

NPL_RAT

Accession

Primary (citable) accession number: Q66H59

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2007
Last sequence update:	October 11, 2004
Last modified:	April 3, 2013
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program