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Protein

N-acetylneuraminate lyase

Gene

Npl

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the cleavage of N-acetylneuraminic acid (sialic acid) to form pyruvate and N-acetylmannosamine via a Schiff base intermediate. It prevents sialic acids from being recycled and returning to the cell surface. Involved in the N-glycolylneuraminic acid (Neu5Gc) degradation pathway (By similarity).By similarity

Catalytic activityi

N-acetylneuraminate = N-acetyl-D-mannosamine + pyruvate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei143 – 1431Involved in proton transfer during cleavageBy similarity
Active sitei173 – 1731Schiff-base intermediate with substrateBy similarity

GO - Molecular functioni

  1. N-acetylneuraminate lyase activity Source: UniProtKB

GO - Biological processi

  1. carbohydrate metabolic process Source: UniProtKB-KW
  2. N-acetylneuraminate catabolic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

Schiff base

Enzyme and pathway databases

ReactomeiREACT_283933. Sialic acid metabolism.
UniPathwayiUPA00629.

Names & Taxonomyi

Protein namesi
Recommended name:
N-acetylneuraminate lyase (EC:4.1.3.3)
Short name:
NALase
Alternative name(s):
N-acetylneuraminate pyruvate-lyase
N-acetylneuraminic acid aldolase
Sialate lyase
Sialate-pyruvate lyase
Sialic acid aldolase
Sialic acid lyase
Gene namesi
Name:Npl
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 13

Organism-specific databases

RGDi1549702. Npl.

Subcellular locationi

  1. Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 320320N-acetylneuraminate lyasePRO_0000273356Add
BLAST

Proteomic databases

PaxDbiQ66H59.
PRIDEiQ66H59.

Expressioni

Gene expression databases

GenevestigatoriQ66H59.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000003713.

Structurei

3D structure databases

ProteinModelPortaliQ66H59.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni51 – 522Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the DapA family. NanA subfamily.Curated

Phylogenomic databases

eggNOGiCOG0329.
GeneTreeiENSGT00530000063604.
HOGENOMiHOG000218206.
HOVERGENiHBG082055.
InParanoidiQ66H59.
KOiK01639.
OMAiLQAFAKP.
OrthoDBiEOG7NKKKP.
PhylomeDBiQ66H59.
TreeFamiTF353639.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR002220. DapA-like.
[Graphical view]
PANTHERiPTHR12128. PTHR12128. 1 hit.
PfamiPF00701. DHDPS. 1 hit.
[Graphical view]
PIRSFiPIRSF001365. DHDPS. 1 hit.
PRINTSiPR00146. DHPICSNTHASE.

Sequencei

Sequence statusi: Complete.

Q66H59-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAFPKKKLQG LVAATITPMT ENGEINFPVI GQYVDYLVKE QGVKNIFVNG
60 70 80 90 100
TTGEGLSLSI SERRQVAEEW VRQGKNKLDQ VVIHVGALNL KESQELAQHA
110 120 130 140 150
AEIGADGIAV IAPFFFKSQN KDALISFLRE VAAAAPALPF YYYHIPSLTG
160 170 180 190 200
VKIRAEELLD GIQDKIPSFQ GLKFSDTDLL DFGQCVDQNH QRQFALLFGV
210 220 230 240 250
DEQLLSALVL GATGAVGSTY NYLGKKTNQM LEAFEQKDLA SALSYQFRIQ
260 270 280 290 300
RFINYVIKLG FGVSQTKAIM TLVSGIPMGP PRLPLQKATQ EFTANAEAKL
310 320
KSLNFLSFPG LKDGNMEACS
Length:320
Mass (Da):35,115
Last modified:October 11, 2004 - v1
Checksum:i3674F314288D8B89
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC082004 mRNA. Translation: AAH82004.1.
RefSeqiNP_001014006.1. NM_001013984.1.
UniGeneiRn.114492.

Genome annotation databases

EnsembliENSRNOT00000003713; ENSRNOP00000003713; ENSRNOG00000002775.
GeneIDi304860.
KEGGirno:304860.
UCSCiRGD:1549702. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC082004 mRNA. Translation: AAH82004.1.
RefSeqiNP_001014006.1. NM_001013984.1.
UniGeneiRn.114492.

3D structure databases

ProteinModelPortaliQ66H59.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000003713.

Proteomic databases

PaxDbiQ66H59.
PRIDEiQ66H59.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000003713; ENSRNOP00000003713; ENSRNOG00000002775.
GeneIDi304860.
KEGGirno:304860.
UCSCiRGD:1549702. rat.

Organism-specific databases

CTDi80896.
RGDi1549702. Npl.

Phylogenomic databases

eggNOGiCOG0329.
GeneTreeiENSGT00530000063604.
HOGENOMiHOG000218206.
HOVERGENiHBG082055.
InParanoidiQ66H59.
KOiK01639.
OMAiLQAFAKP.
OrthoDBiEOG7NKKKP.
PhylomeDBiQ66H59.
TreeFamiTF353639.

Enzyme and pathway databases

UniPathwayiUPA00629.
ReactomeiREACT_283933. Sialic acid metabolism.

Miscellaneous databases

NextBioi653735.
PROiQ66H59.

Gene expression databases

GenevestigatoriQ66H59.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR002220. DapA-like.
[Graphical view]
PANTHERiPTHR12128. PTHR12128. 1 hit.
PfamiPF00701. DHDPS. 1 hit.
[Graphical view]
PIRSFiPIRSF001365. DHDPS. 1 hit.
PRINTSiPR00146. DHPICSNTHASE.
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.

Entry informationi

Entry nameiNPL_RAT
AccessioniPrimary (citable) accession number: Q66H59
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: October 11, 2004
Last modified: April 1, 2015
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.