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Protein

Polypyrimidine tract-binding protein 2

Gene

Ptbp2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

RNA-binding protein which binds to intronic polypyrimidine tracts and mediates negative regulation of exons splicing. May antagonize in a tissue-specific manner the ability of NOVA1 to activate exon selection. In addition to its function in pre-mRNA splicing, plays also a role in the regulation of translation.

GO - Molecular functioni

  • mRNA binding Source: Ensembl
  • pre-mRNA binding Source: RGD

GO - Biological processi

  • cerebellum development Source: RGD
  • mRNA splice site selection Source: Ensembl
  • negative regulation of RNA splicing Source: RGD
  • regulation of neural precursor cell proliferation Source: Ensembl
  • spinal cord development Source: RGD

Keywordsi

Molecular functionRNA-binding
Biological processmRNA processing, mRNA splicing

Names & Taxonomyi

Protein namesi
Recommended name:
Polypyrimidine tract-binding protein 2
Alternative name(s):
Neural polypyrimidine tract-binding protein
PTB-like protein
Gene namesi
Name:Ptbp2
Synonyms:Nptb, Ptblp
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 2

Organism-specific databases

RGDi1359267 Ptbp2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002329301 – 531Polypyrimidine tract-binding protein 2Add BLAST531

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei26PhosphoserineBy similarity1
Modified residuei27PhosphoserineBy similarity1
Modified residuei308PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ66H20
PRIDEiQ66H20

PTM databases

iPTMnetiQ66H20
PhosphoSitePlusiQ66H20

Expressioni

Gene expression databases

BgeeiENSRNOG00000010827

Interactioni

Subunit structurei

Monomer. Identified in a mRNP complex, at least composed of DHX9, DDX3X, ELAVL1, HNRNPU, IGF2BP1, ILF3, PABPC1, PCBP2, PTBP2, STAU1, STAU2, SYNCRIP and YBX1. Part of a ternary complex containing KHSRP and HNRPH1. Interacts with NOVA1; the interaction is direct. Interacts with NOVA2; the interaction is direct (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000015036

Structurei

3D structure databases

ProteinModelPortaliQ66H20
SMRiQ66H20
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini59 – 133RRM 1PROSITE-ProRule annotationAdd BLAST75
Domaini181 – 257RRM 2PROSITE-ProRule annotationAdd BLAST77
Domaini338 – 412RRM 3PROSITE-ProRule annotationAdd BLAST75
Domaini455 – 529RRM 4PROSITE-ProRule annotationAdd BLAST75

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi315 – 324Poly-Ala10

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1190 Eukaryota
ENOG410XPMZ LUCA
GeneTreeiENSGT00550000074508
HOGENOMiHOG000231699
HOVERGENiHBG069548
InParanoidiQ66H20
KOiK14948
OMAiAQQAKLX
OrthoDBiEOG091G0Z5R
PhylomeDBiQ66H20
TreeFamiTF319824

Family and domain databases

CDDicd12778 RRM1_PTBP2, 1 hit
cd12693 RRM2_PTBP1_like, 1 hit
cd12696 RRM3_PTBP2, 1 hit
cd12702 RRM4_PTBP2, 1 hit
Gene3Di3.30.70.330, 4 hits
InterProiView protein in InterPro
IPR006536 HnRNP-L/PTB
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR034798 PTBP1/2/3_RRM2
IPR035002 PTBP2_RRM1
IPR034799 PTBP2_RRM3
IPR034800 PTBP2_RRM4
IPR035979 RBD_domain_sf
IPR000504 RRM_dom
PfamiView protein in Pfam
PF00076 RRM_1, 2 hits
SMARTiView protein in SMART
SM00360 RRM, 4 hits
SUPFAMiSSF54928 SSF54928, 4 hits
TIGRFAMsiTIGR01649 hnRNP-L_PTB, 1 hit
PROSITEiView protein in PROSITE
PS50102 RRM, 4 hits

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q66H20-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDGIVTEVAV GVKRGSDELL SGSVLSSPNS NMSGMVVTAN GNDSKKFKGE
60 70 80 90 100
DKMDGAPSRV LHIRKLPGEV TETEVIALGL PFGKVTNILM LKGKNQAFLE
110 120 130 140 150
LATEEAAITM VNYYSAVTPH LRNQPIYIQY SNHKELKTDN TLNQRAQVVL
160 170 180 190 200
QAVTAVQTAN TPLSGTTVSE SAVTPAQSPV LRIIIDNMYY PVTLDVLHQI
210 220 230 240 250
FSKFGAVLKI ITFTKNNQFQ ALLQYGDPVN AQQAKLALDG QNIYNACCTL
260 270 280 290 300
RIDFSKLVNL NVKYNNDKSR DYTRPDLPSG DGQPALDPAI AAAFAKETSL
310 320 330 340 350
LAVPGALSPL AIPNAAAAAA AAAAGRVGMP GVSAGGNTVL LVSNLNEEMV
360 370 380 390 400
TPQSLFTLFG VYGDVQRVKI LYNKKDSALI QMADGNQSQL AMNHLNGQKM
410 420 430 440 450
YGKIIRVTLS KHQTVQLPRE GLDDQGLTKD FGNSPLHRFK KPGSKNFQNI
460 470 480 490 500
FPPSATLHLS NIPPSVAEED LRTLFANTGG TVKAFKFFQD HKMALLQMAT
510 520 530
VEEAIQALID LHNYNLGENH HLRVSFSKST I
Length:531
Mass (Da):57,489
Last modified:October 11, 2004 - v1
Checksum:i3A93CA4E3E1D6AA3
GO
Isoform 2 (identifier: Q66H20-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     489-489: Q → QR

Show »
Length:532
Mass (Da):57,645
Checksum:i732A3CC8C6D47512
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_018020489Q → QR in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ010585 mRNA Translation: CAB54073.1
BC082076 mRNA Translation: AAH82076.1
AY333750 Genomic DNA Translation: AAQ01148.1
RefSeqiNP_001005555.1, NM_001005555.1 [Q66H20-1]
XP_006233300.1, XM_006233238.3 [Q66H20-2]
UniGeneiRn.64916

Genome annotation databases

EnsembliENSRNOT00000015036; ENSRNOP00000015036; ENSRNOG00000010827 [Q66H20-1]
GeneIDi310820
KEGGirno:310820
UCSCiRGD:1359267 rat [Q66H20-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPTBP2_RAT
AccessioniPrimary (citable) accession number: Q66H20
Secondary accession number(s): Q78ZE9, Q7TNW8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 18, 2006
Last sequence update: October 11, 2004
Last modified: May 23, 2018
This is version 108 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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