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Q66H12

- NAGAB_RAT

UniProt

Q66H12 - NAGAB_RAT

Protein

Alpha-N-acetylgalactosaminidase

Gene

Naga

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 75 (01 Oct 2014)
      Sequence version 1 (11 Oct 2004)
      Previous versions | rss
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    Functioni

    Removes terminal alpha-N-acetylgalactosamine residues from glycolipids and glycopeptides. Required for the breakdown of glycolipids By similarity.By similarity

    Catalytic activityi

    Cleavage of non-reducing alpha-(1->3)-N-acetylgalactosamine residues from human blood group A and AB mucin glycoproteins, Forssman hapten and blood group A lacto series glycolipids.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei154 – 1541SubstrateBy similarity
    Active sitei156 – 1561NucleophileBy similarity
    Binding sitei188 – 1881SubstrateBy similarity
    Binding sitei213 – 2131SubstrateBy similarity
    Active sitei217 – 2171Proton donorBy similarity
    Binding sitei217 – 2171SubstrateBy similarity

    GO - Molecular functioni

    1. alpha-galactosidase activity Source: RefGenome
    2. alpha-N-acetylgalactosaminidase activity Source: UniProtKB

    GO - Biological processi

    1. carbohydrate catabolic process Source: UniProtKB
    2. glycolipid catabolic process Source: UniProtKB
    3. glycoside catabolic process Source: RefGenome
    4. glycosylceramide catabolic process Source: RefGenome
    5. oligosaccharide metabolic process Source: RefGenome

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Protein family/group databases

    CAZyiGH27. Glycoside Hydrolase Family 27.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alpha-N-acetylgalactosaminidase (EC:3.2.1.49)
    Alternative name(s):
    Alpha-galactosidase B
    Gene namesi
    Name:Naga
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 7

    Organism-specific databases

    RGDi1306025. Naga.

    Subcellular locationi

    Lysosome By similarity

    GO - Cellular componenti

    1. cytoplasm Source: RefGenome
    2. lysosome Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Lysosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1717By similarityAdd
    BLAST
    Chaini18 – 415398Alpha-N-acetylgalactosaminidasePRO_0000001020Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi38 ↔ 80By similarity
    Disulfide bondi42 ↔ 49By similarity
    Disulfide bondi127 ↔ 158By similarity
    Glycosylationi177 – 1771N-linked (GlcNAc...)By similarity
    Disulfide bondi187 ↔ 209By similarity
    Glycosylationi201 – 2011N-linked (GlcNAc...)Sequence Analysis
    Modified residuei322 – 3221PhosphoserineBy similarity
    Glycosylationi330 – 3301N-linked (GlcNAc...)Sequence Analysis
    Modified residuei332 – 3321PhosphoserineBy similarity
    Glycosylationi385 – 3851N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi391 – 3911N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Phosphoprotein

    Proteomic databases

    PaxDbiQ66H12.
    PRIDEiQ66H12.

    Expressioni

    Gene expression databases

    GenevestigatoriQ66H12.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    IntActiQ66H12. 1 interaction.
    STRINGi10116.ENSRNOP00000011045.

    Structurei

    3D structure databases

    ProteinModelPortaliQ66H12.
    SMRiQ66H12. Positions 18-402.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni78 – 792Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 27 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG68897.
    GeneTreeiENSGT00390000008751.
    HOGENOMiHOG000161224.
    HOVERGENiHBG001989.
    InParanoidiQ66H12.
    KOiK01204.
    OMAiTNFTVVI.
    OrthoDBiEOG7F24SV.
    PhylomeDBiQ66H12.
    TreeFamiTF312909.

    Family and domain databases

    Gene3Di2.60.40.1180. 1 hit.
    3.20.20.70. 1 hit.
    InterProiIPR013785. Aldolase_TIM.
    IPR013780. Glyco_hydro_13_b.
    IPR002241. Glyco_hydro_27.
    IPR000111. Glyco_hydro_GHD.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF02065. Melibiase. 1 hit.
    [Graphical view]
    PRINTSiPR00740. GLHYDRLASE27.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    PROSITEiPS00512. ALPHA_GALACTOSIDASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q66H12-1 [UniParc]FASTAAdd to Basket

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    MLQKTVLLLA LVAQVLMLEN GLLRTPPMGW LAWERFRCNI NCEEDPKNCI    50
    SERLFMEMAD RLAQDGWRDL GYVYLNIDDC WIGGRDATGR LIPDPKRFPH 100
    GIAFLADYAH SLGLKLGIYE DMGKMTCMGY PGTTLDKVEL DAATFAEWKV 150
    DMLKLDGCYS TPKERAEGYP KMAAALNATG RPIAFSCSWP AYEGGLPPKV 200
    NYTEVAGTCN LWRNYKDIQD SWKSVLSILD WFVKHQDILQ PVSGPGHWND 250
    PDMLLIGNFG LSFDESRAQM ALWTVLAAPL FMSTDLRTIS PQNIDILQNP 300
    LLIKINQDPL GIQGRLIFKS KSHIEVFKRN LSDDASALVF FSRRTDMPYH 350
    FHCSLLELNY PKGSVYEGQN VFTGDIISGL HPETNFTVII NPSGVVMWYL 400
    YPVKGLGIYT MMSQL 415
    Length:415
    Mass (Da):46,871
    Last modified:October 11, 2004 - v1
    Checksum:i57605634B3F74C6E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC082084 mRNA. Translation: AAH82084.1.
    RefSeqiNP_001012120.1. NM_001012120.1.
    XP_006242149.1. XM_006242087.1.
    XP_006242150.1. XM_006242088.1.
    UniGeneiRn.55746.

    Genome annotation databases

    EnsembliENSRNOT00000068020; ENSRNOP00000060590; ENSRNOG00000008064.
    GeneIDi315165.
    KEGGirno:315165.
    UCSCiRGD:1306025. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC082084 mRNA. Translation: AAH82084.1 .
    RefSeqi NP_001012120.1. NM_001012120.1.
    XP_006242149.1. XM_006242087.1.
    XP_006242150.1. XM_006242088.1.
    UniGenei Rn.55746.

    3D structure databases

    ProteinModelPortali Q66H12.
    SMRi Q66H12. Positions 18-402.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q66H12. 1 interaction.
    STRINGi 10116.ENSRNOP00000011045.

    Protein family/group databases

    CAZyi GH27. Glycoside Hydrolase Family 27.

    Proteomic databases

    PaxDbi Q66H12.
    PRIDEi Q66H12.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000068020 ; ENSRNOP00000060590 ; ENSRNOG00000008064 .
    GeneIDi 315165.
    KEGGi rno:315165.
    UCSCi RGD:1306025. rat.

    Organism-specific databases

    CTDi 4668.
    RGDi 1306025. Naga.

    Phylogenomic databases

    eggNOGi NOG68897.
    GeneTreei ENSGT00390000008751.
    HOGENOMi HOG000161224.
    HOVERGENi HBG001989.
    InParanoidi Q66H12.
    KOi K01204.
    OMAi TNFTVVI.
    OrthoDBi EOG7F24SV.
    PhylomeDBi Q66H12.
    TreeFami TF312909.

    Miscellaneous databases

    NextBioi 668806.
    PROi Q66H12.

    Gene expression databases

    Genevestigatori Q66H12.

    Family and domain databases

    Gene3Di 2.60.40.1180. 1 hit.
    3.20.20.70. 1 hit.
    InterProi IPR013785. Aldolase_TIM.
    IPR013780. Glyco_hydro_13_b.
    IPR002241. Glyco_hydro_27.
    IPR000111. Glyco_hydro_GHD.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF02065. Melibiase. 1 hit.
    [Graphical view ]
    PRINTSi PR00740. GLHYDRLASE27.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    PROSITEi PS00512. ALPHA_GALACTOSIDASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Testis.

    Entry informationi

    Entry nameiNAGAB_RAT
    AccessioniPrimary (citable) accession number: Q66H12
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 7, 2005
    Last sequence update: October 11, 2004
    Last modified: October 1, 2014
    This is version 75 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3