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Q66H12

- NAGAB_RAT

UniProt

Q66H12 - NAGAB_RAT

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Protein

Alpha-N-acetylgalactosaminidase

Gene

Naga

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Removes terminal alpha-N-acetylgalactosamine residues from glycolipids and glycopeptides. Required for the breakdown of glycolipids By similarity.By similarity

Catalytic activityi

Cleavage of non-reducing alpha-(1->3)-N-acetylgalactosamine residues from human blood group A and AB mucin glycoproteins, Forssman hapten and blood group A lacto series glycolipids.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei154 – 1541SubstrateBy similarity
Active sitei156 – 1561NucleophileBy similarity
Binding sitei188 – 1881SubstrateBy similarity
Binding sitei213 – 2131SubstrateBy similarity
Active sitei217 – 2171Proton donorBy similarity
Binding sitei217 – 2171SubstrateBy similarity

GO - Molecular functioni

  1. alpha-galactosidase activity Source: RefGenome
  2. alpha-N-acetylgalactosaminidase activity Source: UniProtKB

GO - Biological processi

  1. carbohydrate catabolic process Source: UniProtKB
  2. glycolipid catabolic process Source: UniProtKB
  3. glycoside catabolic process Source: RefGenome
  4. glycosylceramide catabolic process Source: RefGenome
  5. oligosaccharide metabolic process Source: RefGenome
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH27. Glycoside Hydrolase Family 27.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-N-acetylgalactosaminidase (EC:3.2.1.49)
Alternative name(s):
Alpha-galactosidase B
Gene namesi
Name:Naga
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 7

Organism-specific databases

RGDi1306025. Naga.

Subcellular locationi

Lysosome By similarity

GO - Cellular componenti

  1. cytoplasm Source: RefGenome
  2. extracellular vesicular exosome Source: Ensembl
  3. lysosome Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Lysosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1717By similarityAdd
BLAST
Chaini18 – 415398Alpha-N-acetylgalactosaminidasePRO_0000001020Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi38 ↔ 80By similarity
Disulfide bondi42 ↔ 49By similarity
Disulfide bondi127 ↔ 158By similarity
Glycosylationi177 – 1771N-linked (GlcNAc...)By similarity
Disulfide bondi187 ↔ 209By similarity
Glycosylationi201 – 2011N-linked (GlcNAc...)Sequence Analysis
Modified residuei322 – 3221PhosphoserineBy similarity
Glycosylationi330 – 3301N-linked (GlcNAc...)Sequence Analysis
Modified residuei332 – 3321PhosphoserineBy similarity
Glycosylationi385 – 3851N-linked (GlcNAc...)Sequence Analysis
Glycosylationi391 – 3911N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ66H12.
PRIDEiQ66H12.

Expressioni

Gene expression databases

GenevestigatoriQ66H12.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

IntActiQ66H12. 1 interaction.
STRINGi10116.ENSRNOP00000011045.

Structurei

3D structure databases

ProteinModelPortaliQ66H12.
SMRiQ66H12. Positions 18-402.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni78 – 792Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the glycosyl hydrolase 27 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG68897.
GeneTreeiENSGT00390000008751.
HOGENOMiHOG000161224.
HOVERGENiHBG001989.
InParanoidiQ66H12.
KOiK01204.
OMAiTNFTVVI.
OrthoDBiEOG7F24SV.
PhylomeDBiQ66H12.
TreeFamiTF312909.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR013780. Glyco_hydro_13_b.
IPR002241. Glyco_hydro_27.
IPR000111. Glyco_hydro_GHD.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02065. Melibiase. 1 hit.
[Graphical view]
PRINTSiPR00740. GLHYDRLASE27.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00512. ALPHA_GALACTOSIDASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q66H12-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLQKTVLLLA LVAQVLMLEN GLLRTPPMGW LAWERFRCNI NCEEDPKNCI
60 70 80 90 100
SERLFMEMAD RLAQDGWRDL GYVYLNIDDC WIGGRDATGR LIPDPKRFPH
110 120 130 140 150
GIAFLADYAH SLGLKLGIYE DMGKMTCMGY PGTTLDKVEL DAATFAEWKV
160 170 180 190 200
DMLKLDGCYS TPKERAEGYP KMAAALNATG RPIAFSCSWP AYEGGLPPKV
210 220 230 240 250
NYTEVAGTCN LWRNYKDIQD SWKSVLSILD WFVKHQDILQ PVSGPGHWND
260 270 280 290 300
PDMLLIGNFG LSFDESRAQM ALWTVLAAPL FMSTDLRTIS PQNIDILQNP
310 320 330 340 350
LLIKINQDPL GIQGRLIFKS KSHIEVFKRN LSDDASALVF FSRRTDMPYH
360 370 380 390 400
FHCSLLELNY PKGSVYEGQN VFTGDIISGL HPETNFTVII NPSGVVMWYL
410
YPVKGLGIYT MMSQL
Length:415
Mass (Da):46,871
Last modified:October 11, 2004 - v1
Checksum:i57605634B3F74C6E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC082084 mRNA. Translation: AAH82084.1.
RefSeqiNP_001012120.1. NM_001012120.1.
XP_006242149.1. XM_006242087.2.
XP_006242150.1. XM_006242088.2.
UniGeneiRn.55746.

Genome annotation databases

EnsembliENSRNOT00000068020; ENSRNOP00000060590; ENSRNOG00000008064.
GeneIDi315165.
KEGGirno:315165.
UCSCiRGD:1306025. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC082084 mRNA. Translation: AAH82084.1 .
RefSeqi NP_001012120.1. NM_001012120.1.
XP_006242149.1. XM_006242087.2.
XP_006242150.1. XM_006242088.2.
UniGenei Rn.55746.

3D structure databases

ProteinModelPortali Q66H12.
SMRi Q66H12. Positions 18-402.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q66H12. 1 interaction.
STRINGi 10116.ENSRNOP00000011045.

Protein family/group databases

CAZyi GH27. Glycoside Hydrolase Family 27.

Proteomic databases

PaxDbi Q66H12.
PRIDEi Q66H12.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000068020 ; ENSRNOP00000060590 ; ENSRNOG00000008064 .
GeneIDi 315165.
KEGGi rno:315165.
UCSCi RGD:1306025. rat.

Organism-specific databases

CTDi 4668.
RGDi 1306025. Naga.

Phylogenomic databases

eggNOGi NOG68897.
GeneTreei ENSGT00390000008751.
HOGENOMi HOG000161224.
HOVERGENi HBG001989.
InParanoidi Q66H12.
KOi K01204.
OMAi TNFTVVI.
OrthoDBi EOG7F24SV.
PhylomeDBi Q66H12.
TreeFami TF312909.

Miscellaneous databases

NextBioi 668806.
PROi Q66H12.

Gene expression databases

Genevestigatori Q66H12.

Family and domain databases

Gene3Di 2.60.40.1180. 1 hit.
3.20.20.70. 1 hit.
InterProi IPR013785. Aldolase_TIM.
IPR013780. Glyco_hydro_13_b.
IPR002241. Glyco_hydro_27.
IPR000111. Glyco_hydro_GHD.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF02065. Melibiase. 1 hit.
[Graphical view ]
PRINTSi PR00740. GLHYDRLASE27.
SUPFAMi SSF51445. SSF51445. 1 hit.
PROSITEi PS00512. ALPHA_GALACTOSIDASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.

Entry informationi

Entry nameiNAGAB_RAT
AccessioniPrimary (citable) accession number: Q66H12
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: October 11, 2004
Last modified: October 29, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3