Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

F-box only protein 43

Gene

Fbxo43

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Required to establish and maintain the arrest of oocytes at the second meiotic metaphase until fertilization. Probably acts by inhibiting the anaphase-promoting complex/cyclosome (APC/C) ubiquitin ligase. Probably recognizes and binds to some phosphorylated proteins and promotes their ubiquitination and degradation (By similarity).By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri575 – 63763IBR-typeAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Meiosis, Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
F-box only protein 43
Alternative name(s):
Endogenous meiotic inhibitor 2
Gene namesi
Name:Fbxo43
Synonyms:Emi2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi1310225. Fbxo43.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 664664F-box only protein 43PRO_0000247235Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei190 – 1901PhosphothreonineBy similarity

Post-translational modificationi

Phosphorylated on Thr-190 and Ser-289 in response to calcium, which is a prerequisite for ubiquitination and proteasomal degradation.By similarity
Ubiquitinated in response to calcium, which promotes proteasomal degradation.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ66H04.
PRIDEiQ66H04.

PTM databases

iPTMnetiQ66H04.
PhosphoSiteiQ66H04.

Interactioni

Subunit structurei

Part of a SCF (SKP1-cullin-F-box) protein ligase complex. Interacts with CDC20 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000014333.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini445 – 50258F-boxAdd
BLAST

Sequence similaritiesi

Contains 1 F-box domain.Curated
Contains 1 IBR-type zinc finger.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri575 – 63763IBR-typeAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiENOG410IHWX. Eukaryota.
ENOG410XUB4. LUCA.
GeneTreeiENSGT00530000063692.
HOGENOMiHOG000112548.
HOVERGENiHBG081522.
InParanoidiQ66H04.
KOiK10318.
OMAiFEVECIS.
OrthoDBiEOG783MXX.
PhylomeDBiQ66H04.
TreeFamiTF101170.

Family and domain databases

InterProiIPR002867. IBR_dom.
[Graphical view]
PfamiPF01485. IBR. 1 hit.
[Graphical view]
SMARTiSM00647. IBR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q66H04-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPQKHSASHR AGSNMGSSAV TVRCPSLPDF GPTSSFKHRG PHELSETCSF
60 70 80 90 100
SKKEKDPMLT CEHLETPRLG FPNPAVSPSP RKKSVSLRKE KEESPELCET
110 120 130 140 150
PKLRRKKCTL RRRLDLTLTL LKGDSDSQSS SLESNISHSL SLEKRLRDST
160 170 180 190 200
SGFPKEDNFS PLVTSTVKTE DAISSSQNSR LHFSQHKTST IDDSKDDCGL
210 220 230 240 250
FEVECLSPIE GNDFKDSVTH FSDSGLSASD ENTCPELLGS SGSQTTYGAD
260 270 280 290 300
VTTSVTPVSS LIAKIKFHGS QTLASSAEVR DSLSTPEDSG FCSLSWDKSE
310 320 330 340 350
DFLSDQEGSF QELQKHRGTP TVGDLVRQPK HPGRLRRLPT LQEQGSQSET
360 370 380 390 400
DDEMQVAHPS AGVRVGTASG VLESQQGSEE KSGDLTLSFK DLSNTPALQL
410 420 430 440 450
VCELFMKSKR KRFEQADDQE FSEEREEGKI ARLQHVLAGL IGKKMGIERL
460 470 480 490 500
DILTELQYRN LKHILAMVLD SLTAESLYSA WNVSRNWRGI VAQDKRANRR
510 520 530 540 550
RKLFIAQLKA KAQGAAVLRV QDAATRLRLL SRLALRSVQA QAQAPNGQNE
560 570 580 590 600
QAPAQSPWGE VLTPVASSSL THLRSKQEQY LKVARTLFTD EALKPCPRCQ
610 620 630 640 650
SPAKYQPHKK RGLCSRLACG FDFCVLCLCA YHGSEDCRRG SAKGRGSRDV
660
LPGSAQSKRN LKRL
Length:664
Mass (Da):73,228
Last modified:November 14, 2006 - v2
Checksum:iAE73B4502F03C0E2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC082107 mRNA. Translation: AAH82107.2.
RefSeqiNP_001012117.2. NM_001012117.1.
XP_006241597.1. XM_006241535.2.
UniGeneiRn.137608.

Genome annotation databases

EnsembliENSRNOT00000014333; ENSRNOP00000014333; ENSRNOG00000010459.
GeneIDi315034.
KEGGirno:315034.
UCSCiRGD:1310225. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC082107 mRNA. Translation: AAH82107.2.
RefSeqiNP_001012117.2. NM_001012117.1.
XP_006241597.1. XM_006241535.2.
UniGeneiRn.137608.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000014333.

PTM databases

iPTMnetiQ66H04.
PhosphoSiteiQ66H04.

Proteomic databases

PaxDbiQ66H04.
PRIDEiQ66H04.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000014333; ENSRNOP00000014333; ENSRNOG00000010459.
GeneIDi315034.
KEGGirno:315034.
UCSCiRGD:1310225. rat.

Organism-specific databases

CTDi286151.
RGDi1310225. Fbxo43.

Phylogenomic databases

eggNOGiENOG410IHWX. Eukaryota.
ENOG410XUB4. LUCA.
GeneTreeiENSGT00530000063692.
HOGENOMiHOG000112548.
HOVERGENiHBG081522.
InParanoidiQ66H04.
KOiK10318.
OMAiFEVECIS.
OrthoDBiEOG783MXX.
PhylomeDBiQ66H04.
TreeFamiTF101170.

Enzyme and pathway databases

UniPathwayiUPA00143.

Miscellaneous databases

PROiQ66H04.

Family and domain databases

InterProiIPR002867. IBR_dom.
[Graphical view]
PfamiPF01485. IBR. 1 hit.
[Graphical view]
SMARTiSM00647. IBR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.

Entry informationi

Entry nameiFBX43_RAT
AccessioniPrimary (citable) accession number: Q66H04
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: November 14, 2006
Last modified: July 6, 2016
This is version 84 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.