Q66GV6 (BRCC3_XENLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lys-63-specific deubiquitinase BRCC36
EC=3.4.19.-

Alternative name(s):
BRCA1-A complex subunit BRCC36
BRCA1/BRCA2-containing complex subunit 3
BRCA1/BRCA2-containing complex subunit 36
BRISC complex subunit BRCC36

Gene names

Name:	brcc3
Synonyms:	brcc36

Organism

Xenopus laevis (African clawed frog)

Taxonomic identifier

8355 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	261 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains. Does not have activity toward 'Lys-48'-linked polyubiquitin chains. Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the brca1-bard1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). In the BRCA1-A complex, it specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX, antagonizing the rnf8-dependent ubiquitination at double-strand breaks (DSBs). Catalytic subunit of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin in various substrates. Mediates the specific 'Lys-63'-specific deubiquitination associated with the COP9 signalosome complex (CSN), via the interaction of the BRISC complex with the CSN complex By similarity.
Subunit structure	Component of the BRCA1-A complex. Component of the BRISC complex By similarity.
Subcellular location	Nucleus By similarity. Note: Localizes at sites of DNA damage at double-strand breaks (DSBs) By similarity.
Sequence similarities	Belongs to the peptidase M67A family. BRCC36 subfamily. Contains 1 MPN (JAB/Mov34) domain.

Ontologies

Keywords
Biological process	DNA damage DNA repair Ubl conjugation pathway
Cellular component	Nucleus
Ligand	Metal-binding Zinc
Molecular function	Chromatin regulator Hydrolase Metalloprotease Protease
Gene Ontology (GO)
Biological process	G2/M transition DNA damage checkpoint Inferred from sequence or structural similarity. Source: UniProtKB double-strand break repair Inferred from sequence or structural similarity. Source: UniProtKB histone H2A K63-linked deubiquitination Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of DNA repair Inferred from sequence or structural similarity. Source: UniProtKB proteolysis Inferred from electronic annotation. Source: UniProtKB-KW response to ionizing radiation Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	BRCA1-A complex Inferred from sequence or structural similarity. Source: UniProtKB BRISC complex Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW metallopeptidase activity Inferred from sequence or structural similarity. Source: UniProtKB polyubiquitin binding Inferred from sequence or structural similarity. Source: UniProtKB ubiquitin thiolesterase activity Inferred from sequence or structural similarity. Source: UniProtKB ubiquitin-specific protease activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 261	261	Lys-63-specific deubiquitinase BRCC36		PRO_0000373949
Regions
	Motif	92 – 105	14	JAMM motif
Sites
	Metal binding	92	1	Zinc; catalytic By similarity
	Metal binding	94	1	Zinc; catalytic By similarity
	Metal binding	105	1	Zinc; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q66GV6 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 38909EDACD36C5A5	FASTA	261	29,789
	10 20 30 40 50 60 MAVQAVHIQG DAFLVCVTHS LSTEREEVMG LCIGEVDTQK LVHIHSVIIL RRSDKRKDRV 70 80 90 100 110 120 EISPEQLSAA TIEADRLADI TGRPMRVVGW YHSHPHITVW PSHVDVRTQA MYQMMDVGFV 130 140 150 160 170 180 GLIFSCFIED KNTKTGRILY TCFQSVQAQK SSEYERIEVP LHVVPHNTIR KVCLESAVEL 190 200 210 220 230 240 PRILCQEEQD AYRRIHSLGH LDSITKIHNG SVFTKNLCGQ MSAISGPLLQ WLEDRLEQNR 250 260 QRAQELQSEK EQLLQELKTL G

References

[1]	NIH - Xenopus Gene Collection (XGC) project Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC082208 mRNA. Translation: AAH82208.1.
RefSeq	NP_001087827.1. NM_001094358.1.
UniGene	Xl.13095.
3D structure databases
ProteinModelPortal	Q66GV6.
ModBase	Search...
Protein family/group databases
MEROPS	M67.004.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	447688.
KEGG	xla:447688.
Organism-specific databases
CTD	79184.
Xenbase	XB-GENE-5812935. brcc3.
Family and domain databases
InterPro	IPR000555. Mov34_MPN_PAD1. [Graphical view]
KO	K11864.
Pfam	PF01398. Mov34. 1 hit. [Graphical view]
SMART	SM00232. JAB_MPN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BRCC3_XENLA

Accession

Primary (citable) accession number: Q66GV6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	October 11, 2004
Last modified:	November 16, 2011
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program