Reviewed, UniProtKB/Swiss-Prot Q66GS2 (B3GTC_ARATH)

Names and origin

Protein names

Recommended name:
    Probable beta-1,3-galactosyltransferase 12
    EC=2.4.1.-

Gene names

Name:	B3GALT12
Ordered Locus Names:	At2g26100
ORF Names:	T19L18.9

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	371 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Beta-1,3-galactosyltransferase that transfers galactose from UDP-galactose to substrates with a terminal glycosyl residue By similarity.
Cofactor	Manganese By similarity.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein Probable.
Sequence similarities	Belongs to the glycosyltransferase 31 family.
Sequence caution	The sequence AAC31227.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Ligand	Manganese
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein amino acid glycosylation Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	galactosyltransferase activity Inferred from electronic annotation. Source: InterPro manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 371	371	Probable beta-1,3-galactosyltransferase 12		PRO_0000359422
Regions
	Transmembrane	46 – 66	21	Signal-anchor for type II membrane protein Potential
	Compositional bias	12 – 42	31	Ser-rich
Amino acid modifications
	Glycosylation	291	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q66GS2-1 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 17AAC6970A735D6E	FASTA	371	41,217
	10 20 30 40 50 60 MPLFSHRFTT ASSSSPASPS YYNKPSSKTH KPNSSSSSYT SSRIHVAIIF FSLVSVFIGV 70 80 90 100 110 120 AGTIFALSST GPASVYRCGG SKDTSRVVSA SRKLGGDGGN NGVVVERRKL LGFVGIQTGF 130 140 150 160 170 180 DSGDRRTALR STWFPSDPDS LLRLEQATGL AFRFVIGKSK DAKKMAELEK EIKEYRDFVL 190 200 210 220 230 240 LDTEEEYIRL PYKTLAFFKA AFKLFEADYY VKADDDIYLR PDRLATLLAN ERLHSQTYIG 250 260 270 280 290 300 CMKKGPVITD PKLKWYEKQG NLIGNEYFLH AYGPIYVLSA EIVASLAAAR NGSLRMFNNE 310 320 330 340 350 360 DVTIGSWMLA MDVHHEDNRA LCDPHCSPKS IAVWDIPKCS GLCDPESRLK ELHKTDMCSK 370 SPTLPPDDID Q

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Arabidopsis ORF clones." Kim C.J., Chen H., Cheuk R.F., Shinn P., Ecker J.R. Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[3]	"Identification of a novel group of putative Arabidopsis thaliana beta-(1,3)-galactosyltransferases." Qu Y., Egelund J., Gilson P.R., Houghton F., Gleeson P.A., Schultz C.J., Bacic A. Plant Mol. Biol. 68:43-59(2008) [PubMed: 18548197] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC004747 Genomic DNA. Translation: AAC31227.1. Sequence problems. BT015330 mRNA. Translation: AAU05453.1. BT015706 mRNA. Translation: AAU45204.1.
IPI	IPI00529000.
PIR	T02614.
RefSeq	NP_180179.2.
UniGene	At.43077
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT31. Glycosyltransferase Family 31.
Proteomic databases
PRIDE	Q66GS2.
Genome annotation databases
GeneID	817150.
GenomeReviews	Gene locus AT2G26100 in contig CT485783_GR.
KEGG	ath:AT2G26100.
NMPDR	fig|3702.1.peg.9632.
Organism-specific databases
TAIR	At2g26100.
Phylogenomic databases
OMA	Q66GS2. RSTWFPS.
Family and domain databases
InterPro	IPR002659. Glyco_trans_31. [Graphical view]
PANTHER	PTHR11214. Glyco_trans_31. 1 hit.
Pfam	PF01762. Galactosyl_T. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B3GTC_ARATH

Accession

Primary (citable) accession number: Q66GS2
Secondary accession number(s): O80987

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	October 11, 2004
Last modified:	May 5, 2009
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)