Q66GQ3 (PDI16_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein disulfide isomerase-like 1-6
Short name=AtPDIL1-6
EC=5.3.4.1

Alternative name(s):
Protein disulfide isomerase 4
Short name=AtPDI4
Protein disulfide isomerase-like 3-2
Short name=AtPDIL3-2

Gene names

Name:	PDIL1-6
Synonyms:	PDI4, PDIL3-2
Ordered Locus Names:	At3g16110
ORF Names:	MSL1.15

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	534 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Acts as a protein-folding catalyst that interacts with nascent polypeptides to catalyze the formation, isomerization, and reduction or oxidation of disulfide bonds By similarity.
Catalytic activity	Catalyzes the rearrangement of -S-S- bonds in proteins.
Subcellular location	Endoplasmic reticulum lumen Probable.
Tissue specificity	Widely expressed. Ref.6
Sequence similarities	Belongs to the protein disulfide isomerase family. Contains 2 thioredoxin domains.
Sequence caution	The sequence BAB02677.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum
Domain	Redox-active center Repeat Signal
Molecular function	Isomerase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro
Cellular_component	endoplasmic reticulum Inferred from direct assay PubMed 16618929PubMed 22923678. Source: TAIR endoplasmic reticulum lumen Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	protein disulfide isomerase activity Inferred from sequence or structural similarity Ref.6. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 30	30	Potential
	Chain	31 – 534	504	Protein disulfide isomerase-like 1-6		PRO_0000400021
Regions
	Domain	56 – 182	127	Thioredoxin 1
	Domain	407 – 524	118	Thioredoxin 2
	Motif	531 – 534	4	Prevents secretion from ER By similarity
Sites
	Active site	104	1	Nucleophile By similarity
	Active site	446	1	Nucleophile By similarity
	Active site	449	1	Nucleophile By similarity
Amino acid modifications
	Glycosylation	158	1	N-linked (GlcNAc...) Potential
	Glycosylation	415	1	N-linked (GlcNAc...) Potential
	Disulfide bond	446 ↔ 449		Redox-active By similarity
Experimental info
	Sequence conflict	326	1	L → P in BAD94313. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q66GQ3 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 66FA90F57CF5FF85	FASTA	534	59,571
	10 20 30 40 50 60 MLTKPKPNSK FSILFTFLLL LSFLIFVARS SDVAVEAGSE EELDDLEQLL AVDEQLQEER 70 80 90 100 110 120 PEQQSEAETV SKAQRIVVEL NGDNTKRLID GNEYVMVLGY APWCARSAEL MPRFAEAATD 130 140 150 160 170 180 LKEIGSSVLM AKIDGERYSK VASQLEIKGF PTLLLFVNGT SQSYTGGFSS EEIVIWVQKK 190 200 210 220 230 240 TGASTIKLDT VDEASGFLKK HHTFILGLFE KSEDSSGHDE FVKAASLDNE IQFVETSSID 250 260 270 280 290 300 VAKLLFPNLK TNNVFVGLVK TEAEKYTSYD GPCQAEKIVE FLNSNKFPLV TKLTESNTVR 310 320 330 340 350 360 VYSSPVKLQV MVFSKTDDFE SLAQPLEDIA RKFKSKLMLI YIDISNENLA MPFLTLFGIE 370 380 390 400 410 420 DAKKTVVAAF DNNLNSKYLL ESDPSPSNIE EFCFGLAHGT VSAYYKSQPI PDNQNASVVA 430 440 450 460 470 480 VVGRTFDEVV LRSSENVLLE VHTPWCINCE ALSKQVEKLS QHFKGFENLV FARIDASANE 490 500 510 520 530 HPKLTVDDYP TILLYKTGEK ENPLKLSTKS SAKDMAVLIN KELKWKDQSG KDEL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones." Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S. DNA Res. 7:131-135(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Arabidopsis ORF clones." Kim C.J., Chen H., Cheuk R.F., Shinn P., Ecker J.R. Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"Phylogenetic analyses identify 10 classes of the protein disulfide isomerase family in plants, including single-domain protein disulfide isomerase-related proteins." Houston N.L., Fan C., Xiang J.Q., Schulze J.M., Jung R., Boston R.S. Plant Physiol. 137:762-778(2005) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
[6]	"Endoplasmic reticulum stress activates the expression of a sub-group of protein disulfide isomerase genes and AtbZIP60 modulates the response in Arabidopsis thaliana." Lu D.-P., Christopher D.A. Mol. Genet. Genomics 280:199-210(2008) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
[7]	"The protein disulfide isomerase gene family in bread wheat (T. aestivum L.)." d'Aloisio E., Paolacci A.R., Dhanapal A.P., Tanzarella O.A., Porceddu E., Ciaffi M. BMC Plant Biol. 10:101-101(2010) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB012247 Genomic DNA. Translation: BAB02677.1. Sequence problems. CP002686 Genomic DNA. Translation: AEE75772.1. BT015349 mRNA. Translation: AAU05472.1. BT015798 mRNA. Translation: AAU93570.1. AK221388 mRNA. Translation: BAD94313.1.
IPI	IPI00538596.
RefSeq	NP_188232.2. NM_112481.3.
UniGene	At.50187.
3D structure databases
ProteinModelPortal	Q66GQ3.
SMR	Q66GQ3. Positions 77-530.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT3G16110.1-P.
Proteomic databases
PaxDb	Q66GQ3.
PRIDE	Q66GQ3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT3G16110.1; AT3G16110.1; AT3G16110.
GeneID	820856.
KEGG	ath:AT3G16110.
Organism-specific databases
TAIR	At3g16110.
Phylogenomic databases
eggNOG	COG0526.
HOGENOM	HOG000241706.
InParanoid	Q66GQ3.
KO	K09580.
OMA	NEIQFVE.
PhylomeDB	Q66GQ3.
ProtClustDB	CLSN2680753.
Gene expression databases
Genevestigator	Q66GQ3.
Family and domain databases
Gene3D	3.40.30.10. 3 hits.
InterPro	IPR012336. Thioredoxin-like_fold. IPR013766. Thioredoxin_domain. [Graphical view]
Pfam	PF00085. Thioredoxin. 2 hits. [Graphical view]
SUPFAM	SSF52833. Thiordxn-like_fd. 4 hits.
PROSITE	PS00014. ER_TARGET. 1 hit. PS00194. THIOREDOXIN_1. False negative. PS51352. THIOREDOXIN_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PDI16_ARATH

Accession

Primary (citable) accession number: Q66GQ3
Secondary accession number(s): Q56YD5, Q9LW75

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 2, 2010
Last sequence update:	October 11, 2004
Last modified:	May 1, 2013
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program