Reviewed, UniProtKB/Swiss-Prot Q66GP9 (NOS1_ARATH)

Names and origin

Protein names

Recommended name:
    Nitric oxide synthase 1
      Short name=AtNOS1
    EC=1.14.13.39

Gene names

Name:	NOS1
Ordered Locus Names:	At3g47450
ORF Names:	T21L8.200

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	561 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Produces nitric oxide (NO) which is a messenger molecule involved in hormonal signaling and defense responses in plant. Ref.3
Catalytic activity	L-arginine + n NADPH + n H+ + m O2 = citrulline + nitric oxide + n NADP+.
Enzyme regulation	Stimulated by calcium/calmodulin. Inhibited by L-NAME. Not activated by tetrahydrobiopterin (BH4), FAD, FMN, or heme.
Induction	Constitutively expressed. Induced by abscisic acid (ABA) and lipopolysaccharides. Ref.4
Sequence similarities	Belongs to the plant NOS family.
Biophysicochemical properties	Kinetic parameters: KM=12.5 µM for arginine Vmax=5.0 nmol/min/mg enzyme
Sequence caution	The sequence CAB51217.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	leaf development Inferred from mutant phenotype. Source: TAIR nitric oxide biosynthetic process Ref.3 Inferred from direct assay. Source: TAIR oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW regulation of isopentenyl diphosphate biosynthetic process, mevalonate-independent pathway Inferred from mutant phenotype. Source: TAIR regulation of protein metabolic process Inferred from mutant phenotype. Source: TAIR response to oxidative stress Inferred from direct assay. Source: TAIR response to ozone Inferred from mutant phenotype. Source: TAIR thylakoid membrane organization Inferred from mutant phenotype. Source: TAIR
Cellular component	chloroplast Inferred from direct assay. Source: TAIR mitochondrion Inferred from direct assay. Source: TAIR
Molecular function	GTPase activity Inferred from direct assay. Source: TAIR nitric-oxide synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q66GP9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 561	561	Nitric oxide synthase 1		PRO_0000213736

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 35CF40F9E191FDF1	FASTA	561	61,957
	10 20 30 40 50 60 MALRTLSTFP SLPRRHTTTR REPNLTVIYR NPTTSIVCKS IANSEPPVSL SERDGFAAAA 70 80 90 100 110 120 PTPGERFLEN QRAHEAQKVV KKEIKKEKKK KKEEIIARKV VDTSVSCCYG CGAPLQTSDV 130 140 150 160 170 180 DSPGFVDLVT YELKKKHHQL RTMICGRCQL LSHGHMITAV GGNGGYPGGK QFVSADELRE 190 200 210 220 230 240 KLSHLRHEKA LIVKLVDIVD FNGSFLARVR DLVGANPIIL VITKIDLLPK GTDMNCIGDW 250 260 270 280 290 300 VVEVTMRKKL NVLSVHLTSS KSLDGVSGVA SEIQKEKKGR DVYILGAANV GKSAFINALL 310 320 330 340 350 360 KTMAERDPVA AAAQKYKPIQ SAVPGTTLGP IQINAFVGGE KLYDTPGVHL HHRQAAVVHS 370 380 390 400 410 420 DDLPALAPQN RLRGQSFDIS TLPTQSSSSP KGESLNGYTF FWGGLVRIDI LKALPETCFT 430 440 450 460 470 480 FYGPKALEIH AVPTKTATAF YEKELGVLLT PPSGKNQMQE WKGLQSHRLL QIEINDAKRP 490 500 510 520 530 540 ASDVAISGLG WISIEPIRKT RGTEPRDLNE AEHEIHICVS VPKPVEVFLR PTLPIGTSGT 550 560 EWYQYRELTD KEEEVRPKWY F

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana." Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. Nature 408:820-822(2000) [PubMed: 11130713] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Arabidopsis ORF clones." Cheuk R.F., Chen H., Kim C.J., Shinn P., Ecker J.R. Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[3]	"Identification of a plant nitric oxide synthase gene involved in hormonal signaling." Guo F.-Q., Okamoto M., Crawford N.M. Science 302:100-103(2003) [PubMed: 14526079] [Abstract] Cited for: IDENTIFICATION, CHARACTERIZATION, FUNCTION.
[4]	"Innate immunity in Arabidopsis thaliana: lipopolysaccharides activate nitric oxide synthase (NOS) and induce defense genes." Zeidler D., Zaehringer U., Gerber I., Dubery I., Hartung T., Bors W., Hutzler P., Durner J. Proc. Natl. Acad. Sci. U.S.A. 101:15811-15816(2004) [PubMed: 15498873] [Abstract] Cited for: INDUCTION BY LIPOPOLYSACCHARIDES.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL096860 Genomic DNA. Translation: CAB51217.1. Sequence problems. BT015353 mRNA. Translation: AAU05476.1. BT015887 mRNA. Translation: AAU95423.1.
IPI	IPI00520335.
PIR	T13000.
RefSeq	NP_850666.1.
UniGene	At.43334
3D structure databases
SMR	Q66GP9. Positions 189-351.
ModBase	Search...
Proteomic databases
PRIDE	Q66GP9.
Genome annotation databases
GeneID	823899.
GenomeReviews	Gene locus AT3G47450 in contig BA000014_GR.
NMPDR	fig|3702.1.peg.15967.
Organism-specific databases
TAIR	At3g47450.
Phylogenomic databases
HOGENOM	HBG476588.
InParanoid	Q66GP9.
Enzyme and pathway databases
BRENDA	1.14.13.39. 302.
Gene expression databases
Genevestigator	Q66GP9.
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

NOS1_ARATH

Accession

Primary (citable) accession number: Q66GP9
Secondary accession number(s): Q9STX8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	October 11, 2004
Last modified:	February 9, 2010
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)