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Protein

NO-associated protein 1, chloroplastic/mitochondrial

Gene

NOA1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Exhibits cGTPase activity; binds and hydrolyzes specifically GTP (PubMed:18801746). May participate in ribosome assembly and stability and thus regulates protein synthesis in chloroplasts. The GTPase activity requires MgCl2and the presence of either KCl or (NH4)2SO4. Involved in the post-transcriptional regulation of the methylerythritol phosphate (MEP) pathway. Involved in chlorophyll-a fluorescence regulation.1 Publication
May mediate the production or accumulation of nitric oxide (NO) which is a messenger molecule involved in hormonal signaling and defense responses in plant (PubMed:14526079, PubMed:16272429, PubMed:16690168 and PubMed:17351048). Acts as an antisenescence agent. Plays a crucial role in both extracellular calmodulin (ExtCaM)-triggered and salicylic acid (SA)-mediated H2O2-dependent stomatal closure.

Caution

Nitric oxide synthase (NOS) activity is dubious.1 Publication

Catalytic activityi

2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.

Enzyme regulationi

Stimulated by calcium/calmodulin. Inhibited by L-NAME. Not activated by tetrahydrobiopterin (BH4), FAD, FMN, or heme.

Kineticsi

  1. KM=12.5 µM for arginine1 Publication
  2. KM=64.5 µM for GTP (at 37 degrees Celsius)1 Publication
  1. Vmax=5.0 nmol/min/mg enzyme with arginine as substrate1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi108 – 111GTPBy similarity4
Nucleotide bindingi166 – 174GTPBy similarity9
Nucleotide bindingi223 – 226GTPBy similarity4
Nucleotide bindingi260 – 261GTPBy similarity2
Nucleotide bindingi289 – 294GTPBy similarity6

GO - Molecular functioni

  • GTPase activity Source: TAIR
  • GTP binding Source: UniProtKB-KW
  • oxidoreductase activity Source: UniProtKB-KW

GO - Biological processi

  • leaf development Source: TAIR
  • nitric oxide biosynthetic process Source: TAIR
  • plastid organization Source: TAIR
  • regulation of isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway Source: TAIR
  • regulation of protein metabolic process Source: TAIR
  • response to oxidative stress Source: TAIR
  • response to ozone Source: TAIR
  • response to salt stress Source: UniProtKB
  • ribosome biogenesis Source: GO_Central
  • thylakoid membrane organization Source: TAIR

Keywordsi

Molecular functionHydrolase, Oxidoreductase
LigandGTP-binding, NADP, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT3G47450-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
NO-associated protein 1, chloroplastic/mitochondrial
Short name:
AtNOA1
Alternative name(s):
Dubious mitochondrial nitric oxide synthase 1 (EC:1.14.13.39)
Short name:
AtNOS1
GTPase NOA1
Protein RESISTANT TO INHIBITION BY FOSMIDOMYCIN 1
Gene namesi
Name:NOA1
Synonyms:NOS1, RIF1
Ordered Locus Names:At3g47450
ORF Names:T21L8.200
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G47450
TAIRilocus:2099520 AT3G47450

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Mitochondrion, Plastid

Pathology & Biotechi

Disruption phenotypei

Pale seedlings with a delayed development and greening of true leaves resulting in small plants with a characteristic virescent phenotype of pale young leaves but green mature leaves. Loss of NOS activity in mitochondria. Reduced extracellular calmodulin- (ExtCaM-) triggered and salicylic acid (SA)-mediated increase in NO levels and subsequent H2O2-dependent stomatal closure. Faster dark-induced senescence in leaves. Higher accumulation of hydrogen peroxide, superoxide anion, oxidized lipid, and oxidized protein. Increased hypersensitivity to salt stress and methyl viologen (MV) treatment. Enhanced accumulation of Na+ but reduced accumulation of K+ when exposed to NaCl leading to an enhanced sensitivity to salt. Post-transcriptional up-regulation of the methylerythritol phosphate (MEP) pathway. Enhanced resistance to fosmidomycin (FSM). Disturbed chlorophyll-a fluorescence induction in response to temperature variation, accompanied with altered polyamines accumulation.7 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi327T → A: Loss of GTPase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 31Chloroplast and mitochondrionSequence analysisAdd BLAST31
ChainiPRO_000021373632 – 561NO-associated protein 1, chloroplastic/mitochondrialAdd BLAST530

Proteomic databases

PaxDbiQ66GP9
PRIDEiQ66GP9

PTM databases

SwissPalmiQ66GP9

Expressioni

Tissue specificityi

Expressed in aleurone layer and the embryo.1 Publication

Inductioni

Constitutively expressed. Induced by abscisic acid (ABA) and lipopolysaccharides.1 Publication

Gene expression databases

ExpressionAtlasiQ66GP9 baseline and differential
GenevisibleiQ66GP9 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT3G47450.1

Structurei

3D structure databases

ProteinModelPortaliQ66GP9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini175 – 351CP-type GPROSITE-ProRule annotationAdd BLAST177

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi85 – 92Poly-Lys8

Sequence similaritiesi

Belongs to the TRAFAC class YlqF/YawG GTPase family. NOA1 subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1249 Eukaryota
COG1161 LUCA
HOGENOMiHOG000238714
InParanoidiQ66GP9
KOiK13427
OMAiHYNEVQD
OrthoDBiEOG093608XE
PhylomeDBiQ66GP9

Family and domain databases

InterProiView protein in InterPro
IPR030378 G_CP_dom
IPR006073 GTP_binding_domain
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF01926 MMR_HSR1, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS51721 G_CP, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q66GP9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MALRTLSTFP SLPRRHTTTR REPNLTVIYR NPTTSIVCKS IANSEPPVSL
60 70 80 90 100
SERDGFAAAA PTPGERFLEN QRAHEAQKVV KKEIKKEKKK KKEEIIARKV
110 120 130 140 150
VDTSVSCCYG CGAPLQTSDV DSPGFVDLVT YELKKKHHQL RTMICGRCQL
160 170 180 190 200
LSHGHMITAV GGNGGYPGGK QFVSADELRE KLSHLRHEKA LIVKLVDIVD
210 220 230 240 250
FNGSFLARVR DLVGANPIIL VITKIDLLPK GTDMNCIGDW VVEVTMRKKL
260 270 280 290 300
NVLSVHLTSS KSLDGVSGVA SEIQKEKKGR DVYILGAANV GKSAFINALL
310 320 330 340 350
KTMAERDPVA AAAQKYKPIQ SAVPGTTLGP IQINAFVGGE KLYDTPGVHL
360 370 380 390 400
HHRQAAVVHS DDLPALAPQN RLRGQSFDIS TLPTQSSSSP KGESLNGYTF
410 420 430 440 450
FWGGLVRIDI LKALPETCFT FYGPKALEIH AVPTKTATAF YEKELGVLLT
460 470 480 490 500
PPSGKNQMQE WKGLQSHRLL QIEINDAKRP ASDVAISGLG WISIEPIRKT
510 520 530 540 550
RGTEPRDLNE AEHEIHICVS VPKPVEVFLR PTLPIGTSGT EWYQYRELTD
560
KEEEVRPKWY F
Length:561
Mass (Da):61,957
Last modified:October 11, 2004 - v1
Checksum:i35CF40F9E191FDF1
GO
Isoform 2 (identifier: Q66GP9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     443-444: KE → AK

Note: Derived from EST data. No experimental confirmation available.
Show »
Length:561
Mass (Da):61,899
Checksum:i9FCF40F7EB9550E7
GO

Sequence cautioni

The sequence CAB51217 differs from that shown. Reason: Erroneous gene model prediction.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_044128443 – 444KE → AK in isoform 2. Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL096860 Genomic DNA Translation: CAB51217.1 Sequence problems.
CP002686 Genomic DNA Translation: AEE78282.1
CP002686 Genomic DNA Translation: AEE78283.1
BT015353 mRNA Translation: AAU05476.1
BT015887 mRNA Translation: AAU95423.1
AK226195 mRNA Translation: BAE98360.1
DQ539437 mRNA Translation: ABG25038.1
PIRiT13000
RefSeqiNP_190329.2, NM_114613.3 [Q66GP9-2]
NP_850666.1, NM_180335.1 [Q66GP9-1]
UniGeneiAt.43334

Genome annotation databases

EnsemblPlantsiAT3G47450.1; AT3G47450.1; AT3G47450 [Q66GP9-2]
AT3G47450.2; AT3G47450.2; AT3G47450 [Q66GP9-1]
GeneIDi823899
GrameneiAT3G47450.1; AT3G47450.1; AT3G47450 [Q66GP9-2]
AT3G47450.2; AT3G47450.2; AT3G47450 [Q66GP9-1]
KEGGiath:AT3G47450

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiNOA1_ARATH
AccessioniPrimary (citable) accession number: Q66GP9
Secondary accession number(s): F4JBJ3
, Q0WWY8, Q157M8, Q9STX8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: October 11, 2004
Last modified: April 25, 2018
This is version 92 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome