Reviewed,
UniProtKB/Swiss-Prot Q66GP9 (NOS1_ARATH)
Last modified
February 9, 2010.
Version 35.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Nitric oxide synthase 1 Short name=AtNOS1 EC=1.14.13.39 | ||||||
| Gene names |
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| Organism | Arabidopsis thaliana (Mouse-ear cress) [Complete proteome] | ||||||
| Taxonomic identifier | 3702 [NCBI] | ||||||
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis |
Protein attributes
| Sequence length | 561 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Produces nitric oxide (NO) which is a messenger molecule involved in hormonal signaling and defense responses in plant. Ref.3 |
| Catalytic activity | L-arginine + n NADPH + n H+ + m O2 = citrulline + nitric oxide + n NADP+. |
| Enzyme regulation | Stimulated by calcium/calmodulin. Inhibited by L-NAME. Not activated by tetrahydrobiopterin (BH4), FAD, FMN, or heme. |
| Induction | Constitutively expressed. Induced by abscisic acid (ABA) and lipopolysaccharides. Ref.4 |
| Sequence similarities | Belongs to the plant NOS family. |
| Biophysicochemical properties | Kinetic parameters: KM=12.5 µM for arginine Vmax=5.0 nmol/min/mg enzyme |
| Sequence caution | The sequence CAB51217.1 differs from that shown. Reason: Erroneous gene model prediction. |
Ontologies
Alternative products
| This entry describes 1 isoform produced by alternative splicing. [Select] Note: A number of isoforms are produced. According to EST sequences. | ||||||
| Isoform 1 (identifier: Q66GP9-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||
Molecule processing | |||||||
|---|---|---|---|---|---|---|---|
| Chain | 1 – 561 | 561 | Nitric oxide synthase 1 | PRO_0000213736 | |||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana." Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. Tabata S.Nature 408:820-822(2000) [PubMed: 11130713] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia. |
| [2] | "Arabidopsis ORF clones." Cheuk R.F., Chen H., Kim C.J., Shinn P., Ecker J.R. Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia. |
| [3] | "Identification of a plant nitric oxide synthase gene involved in hormonal signaling." Guo F.-Q., Okamoto M., Crawford N.M. Science 302:100-103(2003) [PubMed: 14526079] [Abstract] Cited for: IDENTIFICATION, CHARACTERIZATION, FUNCTION. |
| [4] | "Innate immunity in Arabidopsis thaliana: lipopolysaccharides activate nitric oxide synthase (NOS) and induce defense genes." Zeidler D., Zaehringer U., Gerber I., Dubery I., Hartung T., Bors W., Hutzler P., Durner J. Proc. Natl. Acad. Sci. U.S.A. 101:15811-15816(2004) [PubMed: 15498873] [Abstract] Cited for: INDUCTION BY LIPOPOLYSACCHARIDES. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AL096860 Genomic DNA. Translation: CAB51217.1. Sequence problems. BT015353 mRNA. Translation: AAU05476.1. BT015887 mRNA. Translation: AAU95423.1. |
| IPI | IPI00520335. |
| PIR | T13000. |
| RefSeq | NP_850666.1. |
| UniGene | At.43334 |
3D structure databases | |
| SMR | Q66GP9. Positions 189-351. |
| ModBase | Search... |
Proteomic databases | |
| PRIDE | Q66GP9. |
Genome annotation databases | |
| GeneID | 823899. |
| GenomeReviews | Gene locus AT3G47450 in contig BA000014_GR. |
| NMPDR | fig|3702.1.peg.15967. |
Organism-specific databases | |
| TAIR | At3g47450. |
Phylogenomic databases | |
| HOGENOM | HBG476588. |
| InParanoid | Q66GP9. |
Enzyme and pathway databases | |
| BRENDA | 1.14.13.39. 302. |
Gene expression databases | |
| Genevestigator | Q66GP9. |
Family and domain databases | |
| ProtoNet | Search... |
Entry information
| Entry name | NOS1_ARATH | ||||||||
| Accession | Primary (citable) accession number: Q66GP9 Secondary accession number(s): Q9STX8 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | PPAP (Plant Proteome Annotation Project) | ||||||||
Relevant documents
| Arabidopsis thaliana Arabidopsis thaliana: entries and gene names |
| SIMILARITY comments Index of protein domains and families |

Clusters with


