Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q66GP9 (NOA1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NO-associated protein 1, chloroplastic/mitochondrial

Short name=AtNOA1
Alternative name(s):
Dubious mitochondrial nitric oxide synthase 1
Short name=AtNOS1
EC=1.14.13.39
GTPase NOA1
Protein RESISTANT TO INHIBITION BY FOSMIDOMYCIN 1
Gene names
Name:NOA1
Synonyms:NOS1, RIF1
Ordered Locus Names:At3g47450
ORF Names:T21L8.200
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length561 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Exhibits cGTPase activity; binds and hydrolyzes specifically GTP (Ref.12). May participate in ribosome assembly and stability and thus regulates protein synthesis in chloroplasts. The GTPase activity requires MgCl2and the presence of either KCl or (NH4)2SO4. Involved in the post-transcriptional regulation of the methylerythritol phosphate (MEP) pathway. Involved in chlorophyll-a fluorescence regulation. Ref.6 Ref.8 Ref.9 Ref.11 Ref.12 Ref.13 Ref.14 Ref.15 Ref.16

May mediate the production or accumulation of nitric oxide (NO) which is a messenger molecule involved in hormonal signaling and defense responses in plant (Ref.6, Ref.8, Ref.9 and Ref.11). Acts as an antisenescence agent. Plays a crucial role in both extracellular calmodulin (ExtCaM)-triggered and salicylic acid (SA)-mediated H2O(2)-dependent stomatal closure. Ref.6 Ref.8 Ref.9 Ref.11 Ref.12 Ref.13 Ref.14 Ref.15 Ref.16

Catalytic activity

2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.

Enzyme regulation

Stimulated by calcium/calmodulin. Inhibited by L-NAME. Not activated by tetrahydrobiopterin (BH4), FAD, FMN, or heme.

Subcellular location

Mitochondrion. Plastidchloroplast. Note: Was initialy thought to be mitochondrial (Ref.8) but seems to be chloroplastic (Ref.13). Ref.8 Ref.13

Tissue specificity

Expressed in aleurone layer and the embryo. Ref.10

Induction

Constitutively expressed. Induced by abscisic acid (ABA) and lipopolysaccharides. Ref.7

Disruption phenotype

Pale seedlings with a delayed development and greening of true leaves resulting in small plants with a characteristic virescent phenotype of pale young leaves but green mature leaves. Loss of NOS activity in mitochondria. Reduced extracellular calmodulin- (ExtCaM-) triggered and salicylic acid (SA)-mediated increase in NO levels and subsequent H2O(2)-dependent stomatal closure. Faster dark-induced senescence in leaves. Higher accumulation of hydrogen peroxide, superoxide anion, oxidized lipid, and oxidized protein. Increased hypersensitivity to salt stress and methyl viologen (MV) treatment. Enhanced accumulation of Na+ but reduced accumulation of K+ when exposed to NaCl leading to an enhanced sensitivity to salt. Post-transcriptional up-regulation of the methylerythritol phosphate (MEP) pathway. Enhanced resistance to fosmidomycin (FSM). Disturbed chlorophyll-a fluorescence induction in response to temperature variation, accompanied with altered polyamines accumulation. Ref.8 Ref.9 Ref.11 Ref.13 Ref.14 Ref.15 Ref.16

Sequence similarities

Belongs to the plant NOS family.

Contains 1 G (guanine nucleotide-binding) domain.

Caution

Nitric oxide synthase (NOS) activity is dubious (Ref.12).

Biophysicochemical properties

Kinetic parameters:

KM=12.5 µM for arginine (Ref.6) Ref.6 Ref.12

KM=64.5 µM for GTP (at 37 degrees Celsius, Ref.12)

Vmax=5.0 nmol/min/mg enzyme with arginine as substrate (Ref.6)

Sequence caution

The sequence CAB51217.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
   Cellular componentChloroplast
Mitochondrion
Plastid
   Coding sequence diversityAlternative splicing
   DomainTransit peptide
   LigandGTP-binding
NADP
Nucleotide-binding
   Molecular functionHydrolase
Oxidoreductase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processresponse to salt stress

Inferred from mutant phenotype Ref.11. Source: UniProtKB

   Cellular_componentchloroplast

Inferred from electronic annotation. Source: UniProtKB-SubCell

mitochondrion

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionGTP binding

Inferred from electronic annotation. Source: UniProtKB-KW

hydrolase activity

Inferred from electronic annotation. Source: UniProtKB-KW

nitric-oxide synthase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q66GP9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q66GP9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     443-444: KE → AK
Note: Derived from EST data. No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 3131Chloroplast and mitochondrion Potential
Chain32 – 561530NO-associated protein 1, chloroplastic/mitochondrial
PRO_0000213736

Regions

Domain281 – 35373G
Nucleotide binding108 – 1114GTP By similarity
Nucleotide binding166 – 1749GTP By similarity
Nucleotide binding223 – 2264GTP By similarity
Nucleotide binding260 – 2612GTP By similarity
Nucleotide binding289 – 2946GTP By similarity
Compositional bias85 – 928Poly-Lys

Natural variations

Alternative sequence443 – 4442KE → AK in isoform 2.
VSP_044128

Experimental info

Mutagenesis3271T → A: Loss of GTPase activity. Ref.12

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 11, 2004. Version 1.
Checksum: 35CF40F9E191FDF1

FASTA56161,957
        10         20         30         40         50         60 
MALRTLSTFP SLPRRHTTTR REPNLTVIYR NPTTSIVCKS IANSEPPVSL SERDGFAAAA 

        70         80         90        100        110        120 
PTPGERFLEN QRAHEAQKVV KKEIKKEKKK KKEEIIARKV VDTSVSCCYG CGAPLQTSDV 

       130        140        150        160        170        180 
DSPGFVDLVT YELKKKHHQL RTMICGRCQL LSHGHMITAV GGNGGYPGGK QFVSADELRE 

       190        200        210        220        230        240 
KLSHLRHEKA LIVKLVDIVD FNGSFLARVR DLVGANPIIL VITKIDLLPK GTDMNCIGDW 

       250        260        270        280        290        300 
VVEVTMRKKL NVLSVHLTSS KSLDGVSGVA SEIQKEKKGR DVYILGAANV GKSAFINALL 

       310        320        330        340        350        360 
KTMAERDPVA AAAQKYKPIQ SAVPGTTLGP IQINAFVGGE KLYDTPGVHL HHRQAAVVHS 

       370        380        390        400        410        420 
DDLPALAPQN RLRGQSFDIS TLPTQSSSSP KGESLNGYTF FWGGLVRIDI LKALPETCFT 

       430        440        450        460        470        480 
FYGPKALEIH AVPTKTATAF YEKELGVLLT PPSGKNQMQE WKGLQSHRLL QIEINDAKRP 

       490        500        510        520        530        540 
ASDVAISGLG WISIEPIRKT RGTEPRDLNE AEHEIHICVS VPKPVEVFLR PTLPIGTSGT 

       550        560 
EWYQYRELTD KEEEVRPKWY F 

« Hide

Isoform 2 [UniParc].

Checksum: 9FCF40F7EB9550E7
Show »

FASTA56161,899

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. expand/collapse author list , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Arabidopsis ORF clones."
Cheuk R.F., Chen H., Kim C.J., Shinn P., Ecker J.R.
Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: cv. Columbia.
[4]"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. expand/collapse author list , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: cv. Columbia.
[5]"Role of nitric oxide synthase in plant disease resistance."
Cai X.-Z., Zhang H.-Z.
Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 109-244 (ISOFORM 1/2).
Strain: cv. Columbia.
[6]"Identification of a plant nitric oxide synthase gene involved in hormonal signaling."
Guo F.-Q., Okamoto M., Crawford N.M.
Science 302:100-103(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION, CHARACTERIZATION, FUNCTION AS NOS, BIOPHYSICOCHEMICAL PROPERTIES.
[7]"Innate immunity in Arabidopsis thaliana: lipopolysaccharides activate nitric oxide synthase (NOS) and induce defense genes."
Zeidler D., Zaehringer U., Gerber I., Dubery I., Hartung T., Bors W., Hutzler P., Durner J.
Proc. Natl. Acad. Sci. U.S.A. 101:15811-15816(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION BY LIPOPOLYSACCHARIDES.
[8]"Arabidopsis nitric oxide synthase1 is targeted to mitochondria and protects against oxidative damage and dark-induced senescence."
Guo F.-Q., Crawford N.M.
Plant Cell 17:3436-3450(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION AS NOS, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
[9]"Enhanced sensitivity to oxidative stress in an Arabidopsis nitric oxide synthase mutant."
Zhao M., Zhao X., Wu Y., Zhang L.
J. Plant Physiol. 164:737-745(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION AS NOS, DISRUPTION PHENOTYPE.
Strain: cv. Columbia.
[10]"The Arabidopsis aleurone layer responds to nitric oxide, gibberellin, and abscisic acid and is sufficient and necessary for seed dormancy."
Bethke P.C., Libourel I.G.L., Aoyama N., Chung Y.-Y., Still D.W., Jones R.L.
Plant Physiol. 143:1173-1188(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[11]"Nitric oxide synthase-dependent nitric oxide production is associated with salt tolerance in Arabidopsis."
Zhao M.-G., Tian Q.-Y., Zhang W.-H.
Plant Physiol. 144:206-217(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION AS NOS, DISRUPTION PHENOTYPE.
[12]"AtNOS/AtNOA1 is a functional Arabidopsis thaliana cGTPase and not a nitric-oxide synthase."
Moreau M., Lee G.I., Wang Y., Crane B.R., Klessig D.F.
J. Biol. Chem. 283:32957-32967(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION AS GTPASE, BIOPHYSICOCHEMICAL PROPERTIES, MUTAGENESIS OF THR-327.
[13]"A mutant impaired in the production of plastome-encoded proteins uncovers a mechanism for the homeostasis of isoprenoid biosynthetic enzymes in Arabidopsis plastids."
Flores-Perez U., Sauret-Gueeto S., Gas E., Jarvis P., Rodriguez-Concepcion M.
Plant Cell 20:1303-1315(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION AS GTPASE, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION.
Strain: cv. Columbia.
[14]"A signaling pathway linking nitric oxide production to heterotrimeric G protein and hydrogen peroxide regulates extracellular calmodulin induction of stomatal closure in Arabidopsis."
Li J.-H., Liu Y.-Q., Lue P., Lin H.-F., Bai Y., Wang X.-C., Chen Y.-L.
Plant Physiol. 150:114-124(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN NO ACCUMULATION, DISRUPTION PHENOTYPE.
Strain: cv. Wassilewskija.
[15]"AtNOA1 modulates nitric oxide accumulation and stomatal closure induced by salicylic acid in Arabidopsis."
Sun L.R., Hao F.S., Lu B.S., Ma L.Y.
Plant Signal. Behav. 5:1022-1024(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN NO ACCUMULATION, DISRUPTION PHENOTYPE.
[16]"Atnoa1 mutant Arabidopsis plants induce compensation mechanisms to reduce the negative effects of the mutation."
Majlath I., Szalai G., Papp I., Vankova R., Janda T.
J. Plant Physiol. 168:1184-1190(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[17]"Mechanisms for nitric oxide synthesis in plants."
Crawford N.M.
J. Exp. Bot. 57:471-478(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW ON NOS.
[18]"Hunting for plant nitric oxide synthase provides new evidence of a central role for plastids in nitric oxide metabolism."
Gas E., Flores-Perez U., Sauret-Gueeto S., Rodriguez-Concepcion M.
Plant Cell 21:18-23(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW ON CONTROVERSY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL096860 Genomic DNA. Translation: CAB51217.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE78282.1.
CP002686 Genomic DNA. Translation: AEE78283.1.
BT015353 mRNA. Translation: AAU05476.1.
BT015887 mRNA. Translation: AAU95423.1.
AK226195 mRNA. Translation: BAE98360.1.
DQ539437 mRNA. Translation: ABG25038.1.
PIRT13000.
RefSeqNP_190329.2. NM_114613.3. [Q66GP9-2]
NP_850666.1. NM_180335.1. [Q66GP9-1]
UniGeneAt.43334.

3D structure databases

ProteinModelPortalQ66GP9.
SMRQ66GP9. Positions 176-533.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PaxDbQ66GP9.
PRIDEQ66GP9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT3G47450.2; AT3G47450.2; AT3G47450. [Q66GP9-1]
GeneID823899.
KEGGath:AT3G47450.

Organism-specific databases

TAIRAT3G47450.

Phylogenomic databases

eggNOGCOG1161.
HOGENOMHOG000238714.
InParanoidQ66GP9.
KOK13427.
OMADFNGSWI.
PhylomeDBQ66GP9.

Enzyme and pathway databases

BioCycARA:AT3G47450-MONOMER.
ARA:GQT-1101-MONOMER.

Gene expression databases

GenevestigatorQ66GP9.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR006073. GTP_binding_domain.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF01926. MMR_HSR1. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
ProtoNetSearch...

Other

PROQ66GP9.

Entry information

Entry nameNOA1_ARATH
AccessionPrimary (citable) accession number: Q66GP9
Secondary accession number(s): F4JBJ3 expand/collapse secondary AC list , Q0WWY8, Q157M8, Q9STX8
Entry history
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: October 11, 2004
Last modified: May 14, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names