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Q66GP9

- NOA1_ARATH

UniProt

Q66GP9 - NOA1_ARATH

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Protein

NO-associated protein 1, chloroplastic/mitochondrial

Gene

NOA1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Exhibits cGTPase activity; binds and hydrolyzes specifically GTP (PubMed:18801746). May participate in ribosome assembly and stability and thus regulates protein synthesis in chloroplasts. The GTPase activity requires MgCl2and the presence of either KCl or (NH4)2SO4. Involved in the post-transcriptional regulation of the methylerythritol phosphate (MEP) pathway. Involved in chlorophyll-a fluorescence regulation.1 Publication
May mediate the production or accumulation of nitric oxide (NO) which is a messenger molecule involved in hormonal signaling and defense responses in plant (PubMed:14526079, PubMed:16272429, PubMed:16690168 and PubMed:17351048). Acts as an antisenescence agent. Plays a crucial role in both extracellular calmodulin (ExtCaM)-triggered and salicylic acid (SA)-mediated H2O(2)-dependent stomatal closure.

Catalytic activityi

2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.

Enzyme regulationi

Stimulated by calcium/calmodulin. Inhibited by L-NAME. Not activated by tetrahydrobiopterin (BH4), FAD, FMN, or heme.

Kineticsi

  1. KM=12.5 µM for arginine2 Publications
  2. KM=64.5 µM for GTP (at 37 degrees Celsius, PubMed:18801746)2 Publications

Vmax=5.0 nmol/min/mg enzyme with arginine as substrate2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi108 – 1114GTPBy similarity
Nucleotide bindingi166 – 1749GTPBy similarity
Nucleotide bindingi223 – 2264GTPBy similarity
Nucleotide bindingi260 – 2612GTPBy similarity
Nucleotide bindingi289 – 2946GTPBy similarity

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-KW
  2. hydrolase activity Source: UniProtKB-KW
  3. nitric-oxide synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. response to salt stress Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Oxidoreductase

Keywords - Ligandi

GTP-binding, NADP, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT3G47450-MONOMER.
ARA:GQT-1101-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
NO-associated protein 1, chloroplastic/mitochondrial
Short name:
AtNOA1
Alternative name(s):
Dubious mitochondrial nitric oxide synthase 1 (EC:1.14.13.39)
Short name:
AtNOS1
GTPase NOA1
Protein RESISTANT TO INHIBITION BY FOSMIDOMYCIN 1
Gene namesi
Name:NOA1
Synonyms:NOS1, RIF1
Ordered Locus Names:At3g47450
ORF Names:T21L8.200
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 3

Organism-specific databases

TAIRiAT3G47450.

Subcellular locationi

Mitochondrion. Plastidchloroplast
Note: Was initialy thought to be mitochondrial (PubMed:16272429) but seems to be chloroplastic (PubMed:18469163).2 Publications

GO - Cellular componenti

  1. chloroplast Source: UniProtKB-KW
  2. mitochondrion Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Mitochondrion, Plastid

Pathology & Biotechi

Disruption phenotypei

Pale seedlings with a delayed development and greening of true leaves resulting in small plants with a characteristic virescent phenotype of pale young leaves but green mature leaves. Loss of NOS activity in mitochondria. Reduced extracellular calmodulin- (ExtCaM-) triggered and salicylic acid (SA)-mediated increase in NO levels and subsequent H2O(2)-dependent stomatal closure. Faster dark-induced senescence in leaves. Higher accumulation of hydrogen peroxide, superoxide anion, oxidized lipid, and oxidized protein. Increased hypersensitivity to salt stress and methyl viologen (MV) treatment. Enhanced accumulation of Na+ but reduced accumulation of K+ when exposed to NaCl leading to an enhanced sensitivity to salt. Post-transcriptional up-regulation of the methylerythritol phosphate (MEP) pathway. Enhanced resistance to fosmidomycin (FSM). Disturbed chlorophyll-a fluorescence induction in response to temperature variation, accompanied with altered polyamines accumulation.7 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi327 – 3271T → A: Loss of GTPase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3131Chloroplast and mitochondrionSequence AnalysisAdd
BLAST
Chaini32 – 561530NO-associated protein 1, chloroplastic/mitochondrialPRO_0000213736Add
BLAST

Proteomic databases

PaxDbiQ66GP9.
PRIDEiQ66GP9.

Expressioni

Tissue specificityi

Expressed in aleurone layer and the embryo.1 Publication

Inductioni

Constitutively expressed. Induced by abscisic acid (ABA) and lipopolysaccharides.1 Publication

Gene expression databases

GenevestigatoriQ66GP9.

Structurei

3D structure databases

ProteinModelPortaliQ66GP9.
SMRiQ66GP9. Positions 176-533.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini175 – 351177CP-type GPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi85 – 928Poly-Lys

Sequence similaritiesi

Belongs to the TRAFAC class YlqF/YawG GTPase family. NOA1 subfamily.PROSITE-ProRule annotation
Contains 1 CP-type G (guanine nucleotide-binding) domain.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG1161.
HOGENOMiHOG000238714.
InParanoidiQ66GP9.
KOiK13427.
OMAiDFNGSWI.
PhylomeDBiQ66GP9.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR006073. GTP_binding_domain.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01926. MMR_HSR1. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS51721. G_CP. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q66GP9-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MALRTLSTFP SLPRRHTTTR REPNLTVIYR NPTTSIVCKS IANSEPPVSL
60 70 80 90 100
SERDGFAAAA PTPGERFLEN QRAHEAQKVV KKEIKKEKKK KKEEIIARKV
110 120 130 140 150
VDTSVSCCYG CGAPLQTSDV DSPGFVDLVT YELKKKHHQL RTMICGRCQL
160 170 180 190 200
LSHGHMITAV GGNGGYPGGK QFVSADELRE KLSHLRHEKA LIVKLVDIVD
210 220 230 240 250
FNGSFLARVR DLVGANPIIL VITKIDLLPK GTDMNCIGDW VVEVTMRKKL
260 270 280 290 300
NVLSVHLTSS KSLDGVSGVA SEIQKEKKGR DVYILGAANV GKSAFINALL
310 320 330 340 350
KTMAERDPVA AAAQKYKPIQ SAVPGTTLGP IQINAFVGGE KLYDTPGVHL
360 370 380 390 400
HHRQAAVVHS DDLPALAPQN RLRGQSFDIS TLPTQSSSSP KGESLNGYTF
410 420 430 440 450
FWGGLVRIDI LKALPETCFT FYGPKALEIH AVPTKTATAF YEKELGVLLT
460 470 480 490 500
PPSGKNQMQE WKGLQSHRLL QIEINDAKRP ASDVAISGLG WISIEPIRKT
510 520 530 540 550
RGTEPRDLNE AEHEIHICVS VPKPVEVFLR PTLPIGTSGT EWYQYRELTD
560
KEEEVRPKWY F
Length:561
Mass (Da):61,957
Last modified:October 11, 2004 - v1
Checksum:i35CF40F9E191FDF1
GO
Isoform 2 (identifier: Q66GP9-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     443-444: KE → AK

Note: Derived from EST data. No experimental confirmation available.

Show »
Length:561
Mass (Da):61,899
Checksum:i9FCF40F7EB9550E7
GO

Sequence cautioni

The sequence CAB51217.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei443 – 4442KE → AK in isoform 2. CuratedVSP_044128

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL096860 Genomic DNA. Translation: CAB51217.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE78282.1.
CP002686 Genomic DNA. Translation: AEE78283.1.
BT015353 mRNA. Translation: AAU05476.1.
BT015887 mRNA. Translation: AAU95423.1.
AK226195 mRNA. Translation: BAE98360.1.
DQ539437 mRNA. Translation: ABG25038.1.
PIRiT13000.
RefSeqiNP_190329.2. NM_114613.3. [Q66GP9-2]
NP_850666.1. NM_180335.1. [Q66GP9-1]
UniGeneiAt.43334.

Genome annotation databases

EnsemblPlantsiAT3G47450.2; AT3G47450.2; AT3G47450. [Q66GP9-1]
GeneIDi823899.
KEGGiath:AT3G47450.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL096860 Genomic DNA. Translation: CAB51217.1 . Sequence problems.
CP002686 Genomic DNA. Translation: AEE78282.1 .
CP002686 Genomic DNA. Translation: AEE78283.1 .
BT015353 mRNA. Translation: AAU05476.1 .
BT015887 mRNA. Translation: AAU95423.1 .
AK226195 mRNA. Translation: BAE98360.1 .
DQ539437 mRNA. Translation: ABG25038.1 .
PIRi T13000.
RefSeqi NP_190329.2. NM_114613.3. [Q66GP9-2 ]
NP_850666.1. NM_180335.1. [Q66GP9-1 ]
UniGenei At.43334.

3D structure databases

ProteinModelPortali Q66GP9.
SMRi Q66GP9. Positions 176-533.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PaxDbi Q66GP9.
PRIDEi Q66GP9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT3G47450.2 ; AT3G47450.2 ; AT3G47450 . [Q66GP9-1 ]
GeneIDi 823899.
KEGGi ath:AT3G47450.

Organism-specific databases

TAIRi AT3G47450.

Phylogenomic databases

eggNOGi COG1161.
HOGENOMi HOG000238714.
InParanoidi Q66GP9.
KOi K13427.
OMAi DFNGSWI.
PhylomeDBi Q66GP9.

Enzyme and pathway databases

BioCyci ARA:AT3G47450-MONOMER.
ARA:GQT-1101-MONOMER.

Miscellaneous databases

PROi Q66GP9.

Gene expression databases

Genevestigatori Q66GP9.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR006073. GTP_binding_domain.
IPR027417. P-loop_NTPase.
[Graphical view ]
Pfami PF01926. MMR_HSR1. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
PROSITEi PS51721. G_CP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
    Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
    , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Arabidopsis ORF clones."
    Cheuk R.F., Chen H., Kim C.J., Shinn P., Ecker J.R.
    Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  4. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  5. "Role of nitric oxide synthase in plant disease resistance."
    Cai X.-Z., Zhang H.-Z.
    Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 109-244 (ISOFORM 1/2).
    Strain: cv. Columbia.
  6. "Identification of a plant nitric oxide synthase gene involved in hormonal signaling."
    Guo F.-Q., Okamoto M., Crawford N.M.
    Science 302:100-103(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION, CHARACTERIZATION, FUNCTION AS NOS, BIOPHYSICOCHEMICAL PROPERTIES.
  7. "Innate immunity in Arabidopsis thaliana: lipopolysaccharides activate nitric oxide synthase (NOS) and induce defense genes."
    Zeidler D., Zaehringer U., Gerber I., Dubery I., Hartung T., Bors W., Hutzler P., Durner J.
    Proc. Natl. Acad. Sci. U.S.A. 101:15811-15816(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY LIPOPOLYSACCHARIDES.
  8. "Arabidopsis nitric oxide synthase1 is targeted to mitochondria and protects against oxidative damage and dark-induced senescence."
    Guo F.-Q., Crawford N.M.
    Plant Cell 17:3436-3450(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS NOS, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
  9. "Enhanced sensitivity to oxidative stress in an Arabidopsis nitric oxide synthase mutant."
    Zhao M., Zhao X., Wu Y., Zhang L.
    J. Plant Physiol. 164:737-745(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS NOS, DISRUPTION PHENOTYPE.
    Strain: cv. Columbia.
  10. "The Arabidopsis aleurone layer responds to nitric oxide, gibberellin, and abscisic acid and is sufficient and necessary for seed dormancy."
    Bethke P.C., Libourel I.G.L., Aoyama N., Chung Y.-Y., Still D.W., Jones R.L.
    Plant Physiol. 143:1173-1188(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  11. "Nitric oxide synthase-dependent nitric oxide production is associated with salt tolerance in Arabidopsis."
    Zhao M.-G., Tian Q.-Y., Zhang W.-H.
    Plant Physiol. 144:206-217(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS NOS, DISRUPTION PHENOTYPE.
  12. "AtNOS/AtNOA1 is a functional Arabidopsis thaliana cGTPase and not a nitric-oxide synthase."
    Moreau M., Lee G.I., Wang Y., Crane B.R., Klessig D.F.
    J. Biol. Chem. 283:32957-32967(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS GTPASE, BIOPHYSICOCHEMICAL PROPERTIES, MUTAGENESIS OF THR-327.
  13. "A mutant impaired in the production of plastome-encoded proteins uncovers a mechanism for the homeostasis of isoprenoid biosynthetic enzymes in Arabidopsis plastids."
    Flores-Perez U., Sauret-Gueeto S., Gas E., Jarvis P., Rodriguez-Concepcion M.
    Plant Cell 20:1303-1315(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS GTPASE, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION.
    Strain: cv. Columbia.
  14. "A signaling pathway linking nitric oxide production to heterotrimeric G protein and hydrogen peroxide regulates extracellular calmodulin induction of stomatal closure in Arabidopsis."
    Li J.-H., Liu Y.-Q., Lue P., Lin H.-F., Bai Y., Wang X.-C., Chen Y.-L.
    Plant Physiol. 150:114-124(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN NO ACCUMULATION, DISRUPTION PHENOTYPE.
    Strain: cv. Wassilewskija.
  15. "AtNOA1 modulates nitric oxide accumulation and stomatal closure induced by salicylic acid in Arabidopsis."
    Sun L.R., Hao F.S., Lu B.S., Ma L.Y.
    Plant Signal. Behav. 5:1022-1024(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN NO ACCUMULATION, DISRUPTION PHENOTYPE.
  16. "Atnoa1 mutant Arabidopsis plants induce compensation mechanisms to reduce the negative effects of the mutation."
    Majlath I., Szalai G., Papp I., Vankova R., Janda T.
    J. Plant Physiol. 168:1184-1190(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  17. "Mechanisms for nitric oxide synthesis in plants."
    Crawford N.M.
    J. Exp. Bot. 57:471-478(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW ON NOS.
  18. "Hunting for plant nitric oxide synthase provides new evidence of a central role for plastids in nitric oxide metabolism."
    Gas E., Flores-Perez U., Sauret-Gueeto S., Rodriguez-Concepcion M.
    Plant Cell 21:18-23(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW ON CONTROVERSY.

Entry informationi

Entry nameiNOA1_ARATH
AccessioniPrimary (citable) accession number: Q66GP9
Secondary accession number(s): F4JBJ3
, Q0WWY8, Q157M8, Q9STX8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: October 11, 2004
Last modified: October 29, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3