Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q66GI4

- PRRP1_ARATH

UniProt

Q66GI4 - PRRP1_ARATH

Protein

Proteinaceous RNase P 1, chloroplastic/mitochondrial

Gene

PRORP1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 63 (01 Oct 2014)
      Sequence version 1 (11 Oct 2004)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Endonuclease RNase P responsible for the 5' maturation of tRNA precursors. Preferentially cleaves at the unusual cleavage site, but also able to cleave at the classical cleavage site. Also involved in the maturation of mRNAs in mitochondria.3 Publications

    Catalytic activityi

    Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.2 Publications

    GO - Molecular functioni

    1. ribonuclease P activity Source: TAIR

    GO - Biological processi

    1. RNA phosphodiester bond hydrolysis Source: GOC
    2. RNA phosphodiester bond hydrolysis, endonucleolytic Source: GOC
    3. tRNA processing Source: TAIR

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    tRNA processing

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Proteinaceous RNase P 1, chloroplastic/mitochondrial (EC:3.1.26.5)
    Alternative name(s):
    Pentatricopeptide repeat-containing protein At2g32230
    Gene namesi
    Name:PRORP1
    Ordered Locus Names:At2g32230
    ORF Names:F22D22.2
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G32230.

    Subcellular locationi

    Mitochondrion 1 Publication. Plastidchloroplast 1 Publication

    GO - Cellular componenti

    1. chloroplast Source: TAIR
    2. mitochondrion Source: TAIR

    Keywords - Cellular componenti

    Chloroplast, Mitochondrion, Plastid

    Pathology & Biotechi

    Disruption phenotypei

    Embryo lethality when homozygous.1 Publication

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi474 – 4752DD → AA: Loss of activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 4848Chloroplast and mitochondrionSequence AnalysisAdd
    BLAST
    Chaini49 – 572524Proteinaceous RNase P 1, chloroplastic/mitochondrialPRO_0000356038Add
    BLAST

    Proteomic databases

    PaxDbiQ66GI4.
    PRIDEiQ66GI4.

    Expressioni

    Gene expression databases

    GenevestigatoriQ66GI4.

    Structurei

    Secondary structure

    1
    572
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi96 – 10611
    Turni107 – 1093
    Helixi113 – 12614
    Helixi132 – 14211
    Beta strandi149 – 1524
    Helixi155 – 16915
    Helixi176 – 18914
    Helixi192 – 20413
    Helixi211 – 22313
    Helixi227 – 23913
    Helixi246 – 25813
    Helixi262 – 27514
    Beta strandi277 – 2793
    Helixi281 – 29212
    Helixi294 – 2974
    Helixi306 – 31611
    Beta strandi331 – 3366
    Beta strandi342 – 3443
    Turni345 – 3473
    Helixi358 – 37518
    Helixi378 – 38912
    Beta strandi395 – 3995
    Helixi400 – 4056
    Helixi413 – 42614
    Beta strandi434 – 4385
    Helixi439 – 4424
    Helixi445 – 4473
    Helixi450 – 46112
    Beta strandi465 – 4684
    Helixi474 – 48512
    Beta strandi488 – 4903
    Helixi498 – 5036
    Turni504 – 5063
    Helixi507 – 5159
    Beta strandi516 – 5227
    Turni523 – 5253
    Beta strandi526 – 5305
    Beta strandi547 – 5515
    Turni556 – 5583
    Beta strandi563 – 5686

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4G23X-ray1.98A77-572[»]
    4G24X-ray1.95A77-572[»]
    4G25X-ray2.00A77-572[»]
    4G26X-ray1.75A77-572[»]
    ProteinModelPortaliQ66GI4.
    SMRiQ66GI4. Positions 95-570.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati96 – 13035PPR 1Add
    BLAST
    Repeati136 – 17439PPR 2Add
    BLAST
    Repeati175 – 20935PPR 3Add
    BLAST
    Repeati210 – 24435PPR 4Add
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi316 – 3194Poly-Gly

    Sequence similaritiesi

    Belongs to the PPR family. P subfamily.Curated
    Contains 4 PPR (pentatricopeptide) repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Transit peptide

    Phylogenomic databases

    eggNOGiNOG68490.
    HOGENOMiHOG000006117.
    InParanoidiQ66GI4.
    KOiK18213.
    OMAiQWVCATR.
    PhylomeDBiQ66GI4.

    Family and domain databases

    InterProiIPR002885. Pentatricopeptide_repeat.
    [Graphical view]
    PfamiPF01535. PPR. 1 hit.
    PF13812. PPR_3. 1 hit.
    [Graphical view]
    PROSITEiPS51375. PPR. 3 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q66GI4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLRLTCFTPS FSRACCPLFA MMLKVPSVHL HHPRFSPFRF YHTSLLVKGT    50
    RDRRLILVER SRHLCTLPLA AAKQSAASPS ENLSRKAKKK AIQQSPEALL 100
    KQKLDMCSKK GDVLEALRLY DEARRNGVQL SQYHYNVLLY VCSLAEAATE 150
    SSPNPGLSRG FDIFKQMIVD KVVPNEATFT NGARLAVAKD DPEMAFDMVK 200
    QMKAFGIQPR LRSYGPALFG FCRKGDADKA YEVDAHMVES EVVPEEPELA 250
    ALLKVSMDTK NADKVYKTLQ RLRDLVRQVS KSTFDMIEEW FKSEVATKTG 300
    VKKWDVKKIR DAVVSGGGGW HGQGWLGTGK WNVKRTEMDE NGVCKCCKEK 350
    LVCIDINPVE TETFAASLTR LACEREVKAN FNQFQEWLER HGPFDAVIDG 400
    ANMGLVNQRS FSFFQLNNTV QRCQQISPSK RLPLVILHKS RVNGGPATYP 450
    KNRALLEKWK NAGALYATPP GSNDDWYWLY AAVSCKCLLV TNDEMRDHLF 500
    QLLGNSFFPR WKEKHQVRIS VTREDGLKLN MPPPYSIVIQ ESEDGTWHVP 550
    MSVEDDLQTS RQWLCAKRSK TP 572
    Length:572
    Mass (Da):64,877
    Last modified:October 11, 2004 - v1
    Checksum:i80857002D71F42FA
    GO

    Sequence cautioni

    The sequence AAD15382.1 differs from that shown. Reason: Erroneous gene model prediction.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti250 – 2501A → V in AAM13880. (PubMed:14593172)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC006223 Genomic DNA. Translation: AAD15382.1. Sequence problems.
    CP002685 Genomic DNA. Translation: AEC08652.1.
    AY091059 mRNA. Translation: AAM13880.1.
    BT015418 mRNA. Translation: AAU05541.1.
    PIRiE84730.
    RefSeqiNP_850186.1. NM_179855.2.
    UniGeneiAt.38106.

    Genome annotation databases

    EnsemblPlantsiAT2G32230.1; AT2G32230.1; AT2G32230.
    GeneIDi817782.
    KEGGiath:AT2G32230.

    Cross-referencesi

    Web resourcesi

    Arabidopsis PPR Protein Database

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC006223 Genomic DNA. Translation: AAD15382.1 . Sequence problems.
    CP002685 Genomic DNA. Translation: AEC08652.1 .
    AY091059 mRNA. Translation: AAM13880.1 .
    BT015418 mRNA. Translation: AAU05541.1 .
    PIRi E84730.
    RefSeqi NP_850186.1. NM_179855.2.
    UniGenei At.38106.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4G23 X-ray 1.98 A 77-572 [» ]
    4G24 X-ray 1.95 A 77-572 [» ]
    4G25 X-ray 2.00 A 77-572 [» ]
    4G26 X-ray 1.75 A 77-572 [» ]
    ProteinModelPortali Q66GI4.
    SMRi Q66GI4. Positions 95-570.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PaxDbi Q66GI4.
    PRIDEi Q66GI4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G32230.1 ; AT2G32230.1 ; AT2G32230 .
    GeneIDi 817782.
    KEGGi ath:AT2G32230.

    Organism-specific databases

    GeneFarmi 4941. 436.
    TAIRi AT2G32230.

    Phylogenomic databases

    eggNOGi NOG68490.
    HOGENOMi HOG000006117.
    InParanoidi Q66GI4.
    KOi K18213.
    OMAi QWVCATR.
    PhylomeDBi Q66GI4.

    Gene expression databases

    Genevestigatori Q66GI4.

    Family and domain databases

    InterProi IPR002885. Pentatricopeptide_repeat.
    [Graphical view ]
    Pfami PF01535. PPR. 1 hit.
    PF13812. PPR_3. 1 hit.
    [Graphical view ]
    PROSITEi PS51375. PPR. 3 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Arabidopsis ORF clones."
      Cheuk R.F., Chen H., Kim C.J., Shinn P., Ecker J.R.
      Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Genome-wide analysis of Arabidopsis pentatricopeptide repeat proteins reveals their essential role in organelle biogenesis."
      Lurin C., Andres C., Aubourg S., Bellaoui M., Bitton F., Bruyere C., Caboche M., Debast C., Gualberto J., Hoffmann B., Lecharny A., Le Ret M., Martin-Magniette M.-L., Mireau H., Peeters N., Renou J.-P., Szurek B., Taconnat L., Small I.
      Plant Cell 16:2089-2103(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY.
    6. Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, MUTAGENESIS OF 474-ASP-ASP-475.
    7. "Plant mitochondria use two pathways for the biogenesis of tRNAHis."
      Placido A., Sieber F., Gobert A., Gallerani R., Giege P., Marechal-Drouard L.
      Nucleic Acids Res. 38:7711-7717(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    8. "PRORP proteins support RNase P activity in both organelles and the nucleus in Arabidopsis."
      Gutmann B., Gobert A., Giege P.
      Genes Dev. 26:1022-1027(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY.

    Entry informationi

    Entry nameiPRRP1_ARATH
    AccessioniPrimary (citable) accession number: Q66GI4
    Secondary accession number(s): Q8RWW5, Q9SKX7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 16, 2008
    Last sequence update: October 11, 2004
    Last modified: October 1, 2014
    This is version 63 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3