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Q66GI4 (PRRP1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Proteinaceous RNase P 1, chloroplastic/mitochondrial

EC=3.1.26.5
Alternative name(s):
Pentatricopeptide repeat-containing protein At2g32230
Gene names
Name:PRORP1
Ordered Locus Names:At2g32230
ORF Names:F22D22.2
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length572 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Endonuclease RNase P responsible for the 5' maturation of tRNA precursors. Preferentially cleaves at the unusual cleavage site, but also able to cleave at the classical cleavage site. Also involved in the maturation of mRNAs in mitochondria. Ref.6 Ref.7 Ref.8

Catalytic activity

Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor. Ref.6 Ref.8

Subcellular location

Mitochondrion. Plastidchloroplast Ref.6.

Disruption phenotype

Embryo lethality when homozygous. Ref.6

Sequence similarities

Belongs to the PPR family. P subfamily.

Contains 4 PPR (pentatricopeptide) repeats.

Sequence caution

The sequence AAD15382.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 4848Chloroplast and mitochondrion Potential
Chain49 – 572524Proteinaceous RNase P 1, chloroplastic/mitochondrial
PRO_0000356038

Regions

Repeat96 – 13035PPR 1
Repeat136 – 17439PPR 2
Repeat175 – 20935PPR 3
Repeat210 – 24435PPR 4
Compositional bias316 – 3194Poly-Gly

Experimental info

Mutagenesis474 – 4752DD → AA: Loss of activity. Ref.6
Sequence conflict2501A → V in AAM13880. Ref.3

Secondary structure

........................................................................ 572
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q66GI4 [UniParc].

Last modified October 11, 2004. Version 1.
Checksum: 80857002D71F42FA

FASTA57264,877
        10         20         30         40         50         60 
MLRLTCFTPS FSRACCPLFA MMLKVPSVHL HHPRFSPFRF YHTSLLVKGT RDRRLILVER 

        70         80         90        100        110        120 
SRHLCTLPLA AAKQSAASPS ENLSRKAKKK AIQQSPEALL KQKLDMCSKK GDVLEALRLY 

       130        140        150        160        170        180 
DEARRNGVQL SQYHYNVLLY VCSLAEAATE SSPNPGLSRG FDIFKQMIVD KVVPNEATFT 

       190        200        210        220        230        240 
NGARLAVAKD DPEMAFDMVK QMKAFGIQPR LRSYGPALFG FCRKGDADKA YEVDAHMVES 

       250        260        270        280        290        300 
EVVPEEPELA ALLKVSMDTK NADKVYKTLQ RLRDLVRQVS KSTFDMIEEW FKSEVATKTG 

       310        320        330        340        350        360 
VKKWDVKKIR DAVVSGGGGW HGQGWLGTGK WNVKRTEMDE NGVCKCCKEK LVCIDINPVE 

       370        380        390        400        410        420 
TETFAASLTR LACEREVKAN FNQFQEWLER HGPFDAVIDG ANMGLVNQRS FSFFQLNNTV 

       430        440        450        460        470        480 
QRCQQISPSK RLPLVILHKS RVNGGPATYP KNRALLEKWK NAGALYATPP GSNDDWYWLY 

       490        500        510        520        530        540 
AAVSCKCLLV TNDEMRDHLF QLLGNSFFPR WKEKHQVRIS VTREDGLKLN MPPPYSIVIQ 

       550        560        570 
ESEDGTWHVP MSVEDDLQTS RQWLCAKRSK TP 

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"Arabidopsis ORF clones."
Cheuk R.F., Chen H., Kim C.J., Shinn P., Ecker J.R.
Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Genome-wide analysis of Arabidopsis pentatricopeptide repeat proteins reveals their essential role in organelle biogenesis."
Lurin C., Andres C., Aubourg S., Bellaoui M., Bitton F., Bruyere C., Caboche M., Debast C., Gualberto J., Hoffmann B., Lecharny A., Le Ret M., Martin-Magniette M.-L., Mireau H., Peeters N., Renou J.-P., Szurek B., Taconnat L., Small I.
Plant Cell 16:2089-2103(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY.
[6]"A single Arabidopsis organellar protein has RNase P activity."
Gobert A., Gutmann B., Taschner A., Goessringer M., Holzmann J., Hartmann R.K., Rossmanith W., Giege P.
Nat. Struct. Mol. Biol. 17:740-744(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, MUTAGENESIS OF 474-ASP-ASP-475.
[7]"Plant mitochondria use two pathways for the biogenesis of tRNAHis."
Placido A., Sieber F., Gobert A., Gallerani R., Giege P., Marechal-Drouard L.
Nucleic Acids Res. 38:7711-7717(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[8]"PRORP proteins support RNase P activity in both organelles and the nucleus in Arabidopsis."
Gutmann B., Gobert A., Giege P.
Genes Dev. 26:1022-1027(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, CATALYTIC ACTIVITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC006223 Genomic DNA. Translation: AAD15382.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC08652.1.
AY091059 mRNA. Translation: AAM13880.1.
BT015418 mRNA. Translation: AAU05541.1.
PIRE84730.
RefSeqNP_850186.1. NM_179855.2.
UniGeneAt.38106.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4G23X-ray1.98A77-572[»]
4G24X-ray1.95A77-572[»]
4G25X-ray2.00A77-572[»]
4G26X-ray1.75A77-572[»]
ProteinModelPortalQ66GI4.
SMRQ66GI4. Positions 95-570.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PaxDbQ66GI4.
PRIDEQ66GI4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G32230.1; AT2G32230.1; AT2G32230.
GeneID817782.
KEGGath:AT2G32230.

Organism-specific databases

GeneFarm4941. 436.
TAIRAT2G32230.

Phylogenomic databases

eggNOGNOG68490.
HOGENOMHOG000006117.
InParanoidQ66GI4.
KOK18213.
OMAQWVCATR.
PhylomeDBQ66GI4.

Gene expression databases

GenevestigatorQ66GI4.

Family and domain databases

InterProIPR002885. Pentatricopeptide_repeat.
[Graphical view]
PfamPF01535. PPR. 1 hit.
PF13812. PPR_3. 1 hit.
[Graphical view]
PROSITEPS51375. PPR. 3 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePRRP1_ARATH
AccessionPrimary (citable) accession number: Q66GI4
Secondary accession number(s): Q8RWW5, Q9SKX7
Entry history
Integrated into UniProtKB/Swiss-Prot: December 16, 2008
Last sequence update: October 11, 2004
Last modified: July 9, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names