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Q66GI4

- PRRP1_ARATH

UniProt

Q66GI4 - PRRP1_ARATH

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Protein

Proteinaceous RNase P 1, chloroplastic/mitochondrial

Gene

PRORP1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Endonuclease RNase P responsible for the 5' maturation of tRNA precursors. Preferentially cleaves at the unusual cleavage site, but also able to cleave at the classical cleavage site. Also involved in the maturation of mRNAs in mitochondria.3 Publications

Catalytic activityi

Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.2 Publications

GO - Molecular functioni

  1. ribonuclease P activity Source: TAIR

GO - Biological processi

  1. RNA phosphodiester bond hydrolysis Source: GOC
  2. RNA phosphodiester bond hydrolysis, endonucleolytic Source: GOC
  3. tRNA processing Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

tRNA processing

Names & Taxonomyi

Protein namesi
Recommended name:
Proteinaceous RNase P 1, chloroplastic/mitochondrial (EC:3.1.26.5)
Alternative name(s):
Pentatricopeptide repeat-containing protein At2g32230
Gene namesi
Name:PRORP1
Ordered Locus Names:At2g32230
ORF Names:F22D22.2
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G32230.

Subcellular locationi

Mitochondrion 1 Publication. Plastidchloroplast 1 Publication

GO - Cellular componenti

  1. chloroplast Source: TAIR
  2. mitochondrion Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Mitochondrion, Plastid

Pathology & Biotechi

Disruption phenotypei

Embryo lethality when homozygous.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi474 – 4752DD → AA: Loss of activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4848Chloroplast and mitochondrionSequence AnalysisAdd
BLAST
Chaini49 – 572524Proteinaceous RNase P 1, chloroplastic/mitochondrialPRO_0000356038Add
BLAST

Proteomic databases

PaxDbiQ66GI4.
PRIDEiQ66GI4.

Expressioni

Gene expression databases

GenevestigatoriQ66GI4.

Structurei

Secondary structure

1
572
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi96 – 10611Combined sources
Turni107 – 1093Combined sources
Helixi113 – 12614Combined sources
Helixi132 – 14211Combined sources
Beta strandi149 – 1524Combined sources
Helixi155 – 16915Combined sources
Helixi176 – 18914Combined sources
Helixi192 – 20413Combined sources
Helixi211 – 22313Combined sources
Helixi227 – 23913Combined sources
Helixi246 – 25813Combined sources
Helixi262 – 27514Combined sources
Beta strandi277 – 2793Combined sources
Helixi281 – 29212Combined sources
Helixi294 – 2974Combined sources
Helixi306 – 31611Combined sources
Beta strandi331 – 3366Combined sources
Beta strandi342 – 3443Combined sources
Turni345 – 3473Combined sources
Helixi358 – 37518Combined sources
Helixi378 – 38912Combined sources
Beta strandi395 – 3995Combined sources
Helixi400 – 4056Combined sources
Helixi413 – 42614Combined sources
Beta strandi434 – 4385Combined sources
Helixi439 – 4424Combined sources
Helixi445 – 4473Combined sources
Helixi450 – 46112Combined sources
Beta strandi465 – 4684Combined sources
Helixi474 – 48512Combined sources
Beta strandi488 – 4903Combined sources
Helixi498 – 5036Combined sources
Turni504 – 5063Combined sources
Helixi507 – 5159Combined sources
Beta strandi516 – 5227Combined sources
Turni523 – 5253Combined sources
Beta strandi526 – 5305Combined sources
Beta strandi547 – 5515Combined sources
Turni556 – 5583Combined sources
Beta strandi563 – 5686Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4G23X-ray1.98A77-572[»]
4G24X-ray1.95A77-572[»]
4G25X-ray2.00A77-572[»]
4G26X-ray1.75A77-572[»]
ProteinModelPortaliQ66GI4.
SMRiQ66GI4. Positions 95-570.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati96 – 13035PPR 1Add
BLAST
Repeati136 – 17439PPR 2Add
BLAST
Repeati175 – 20935PPR 3Add
BLAST
Repeati210 – 24435PPR 4Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi316 – 3194Poly-Gly

Sequence similaritiesi

Belongs to the PPR family. P subfamily.Curated
Contains 4 PPR (pentatricopeptide) repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transit peptide

Phylogenomic databases

eggNOGiNOG68490.
HOGENOMiHOG000006117.
InParanoidiQ66GI4.
KOiK18213.
OMAiQWVCATR.
PhylomeDBiQ66GI4.

Family and domain databases

InterProiIPR002885. Pentatricopeptide_repeat.
[Graphical view]
PfamiPF01535. PPR. 1 hit.
PF13812. PPR_3. 1 hit.
[Graphical view]
PROSITEiPS51375. PPR. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q66GI4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLRLTCFTPS FSRACCPLFA MMLKVPSVHL HHPRFSPFRF YHTSLLVKGT
60 70 80 90 100
RDRRLILVER SRHLCTLPLA AAKQSAASPS ENLSRKAKKK AIQQSPEALL
110 120 130 140 150
KQKLDMCSKK GDVLEALRLY DEARRNGVQL SQYHYNVLLY VCSLAEAATE
160 170 180 190 200
SSPNPGLSRG FDIFKQMIVD KVVPNEATFT NGARLAVAKD DPEMAFDMVK
210 220 230 240 250
QMKAFGIQPR LRSYGPALFG FCRKGDADKA YEVDAHMVES EVVPEEPELA
260 270 280 290 300
ALLKVSMDTK NADKVYKTLQ RLRDLVRQVS KSTFDMIEEW FKSEVATKTG
310 320 330 340 350
VKKWDVKKIR DAVVSGGGGW HGQGWLGTGK WNVKRTEMDE NGVCKCCKEK
360 370 380 390 400
LVCIDINPVE TETFAASLTR LACEREVKAN FNQFQEWLER HGPFDAVIDG
410 420 430 440 450
ANMGLVNQRS FSFFQLNNTV QRCQQISPSK RLPLVILHKS RVNGGPATYP
460 470 480 490 500
KNRALLEKWK NAGALYATPP GSNDDWYWLY AAVSCKCLLV TNDEMRDHLF
510 520 530 540 550
QLLGNSFFPR WKEKHQVRIS VTREDGLKLN MPPPYSIVIQ ESEDGTWHVP
560 570
MSVEDDLQTS RQWLCAKRSK TP
Length:572
Mass (Da):64,877
Last modified:October 11, 2004 - v1
Checksum:i80857002D71F42FA
GO

Sequence cautioni

The sequence AAD15382.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti250 – 2501A → V in AAM13880. (PubMed:14593172)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC006223 Genomic DNA. Translation: AAD15382.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC08652.1.
AY091059 mRNA. Translation: AAM13880.1.
BT015418 mRNA. Translation: AAU05541.1.
PIRiE84730.
RefSeqiNP_850186.1. NM_179855.2.
UniGeneiAt.38106.

Genome annotation databases

EnsemblPlantsiAT2G32230.1; AT2G32230.1; AT2G32230.
GeneIDi817782.
KEGGiath:AT2G32230.

Cross-referencesi

Web resourcesi

Arabidopsis PPR Protein Database

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC006223 Genomic DNA. Translation: AAD15382.1 . Sequence problems.
CP002685 Genomic DNA. Translation: AEC08652.1 .
AY091059 mRNA. Translation: AAM13880.1 .
BT015418 mRNA. Translation: AAU05541.1 .
PIRi E84730.
RefSeqi NP_850186.1. NM_179855.2.
UniGenei At.38106.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4G23 X-ray 1.98 A 77-572 [» ]
4G24 X-ray 1.95 A 77-572 [» ]
4G25 X-ray 2.00 A 77-572 [» ]
4G26 X-ray 1.75 A 77-572 [» ]
ProteinModelPortali Q66GI4.
SMRi Q66GI4. Positions 95-570.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PaxDbi Q66GI4.
PRIDEi Q66GI4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G32230.1 ; AT2G32230.1 ; AT2G32230 .
GeneIDi 817782.
KEGGi ath:AT2G32230.

Organism-specific databases

GeneFarmi 4941. 436.
TAIRi AT2G32230.

Phylogenomic databases

eggNOGi NOG68490.
HOGENOMi HOG000006117.
InParanoidi Q66GI4.
KOi K18213.
OMAi QWVCATR.
PhylomeDBi Q66GI4.

Gene expression databases

Genevestigatori Q66GI4.

Family and domain databases

InterProi IPR002885. Pentatricopeptide_repeat.
[Graphical view ]
Pfami PF01535. PPR. 1 hit.
PF13812. PPR_3. 1 hit.
[Graphical view ]
PROSITEi PS51375. PPR. 3 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Arabidopsis ORF clones."
    Cheuk R.F., Chen H., Kim C.J., Shinn P., Ecker J.R.
    Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Genome-wide analysis of Arabidopsis pentatricopeptide repeat proteins reveals their essential role in organelle biogenesis."
    Lurin C., Andres C., Aubourg S., Bellaoui M., Bitton F., Bruyere C., Caboche M., Debast C., Gualberto J., Hoffmann B., Lecharny A., Le Ret M., Martin-Magniette M.-L., Mireau H., Peeters N., Renou J.-P., Szurek B., Taconnat L., Small I.
    Plant Cell 16:2089-2103(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY.
  6. Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, MUTAGENESIS OF 474-ASP-ASP-475.
  7. "Plant mitochondria use two pathways for the biogenesis of tRNAHis."
    Placido A., Sieber F., Gobert A., Gallerani R., Giege P., Marechal-Drouard L.
    Nucleic Acids Res. 38:7711-7717(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "PRORP proteins support RNase P activity in both organelles and the nucleus in Arabidopsis."
    Gutmann B., Gobert A., Giege P.
    Genes Dev. 26:1022-1027(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY.

Entry informationi

Entry nameiPRRP1_ARATH
AccessioniPrimary (citable) accession number: Q66GI4
Secondary accession number(s): Q8RWW5, Q9SKX7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 16, 2008
Last sequence update: October 11, 2004
Last modified: November 26, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3