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Protein

Proteinaceous RNase P 1, chloroplastic/mitochondrial

Gene

PRORP1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Endonuclease RNase P responsible for the 5' maturation of tRNA precursors. Preferentially cleaves at the unusual cleavage site, but also able to cleave at the classical cleavage site. Also involved in the maturation of mRNAs in mitochondria.3 Publications

Catalytic activityi

Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.2 Publications

GO - Molecular functioni

  • ribonuclease P activity Source: TAIR

GO - Biological processi

  • tRNA 5'-leader removal Source: TAIR
  • tRNA processing Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

tRNA processing

Names & Taxonomyi

Protein namesi
Recommended name:
Proteinaceous RNase P 1, chloroplastic/mitochondrial (EC:3.1.26.5)
Alternative name(s):
Pentatricopeptide repeat-containing protein At2g32230
Gene namesi
Name:PRORP1
Ordered Locus Names:At2g32230
ORF Names:F22D22.2
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G32230.

Subcellular locationi

GO - Cellular componenti

  • chloroplast Source: TAIR
  • mitochondrion Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Mitochondrion, Plastid

Pathology & Biotechi

Disruption phenotypei

Embryo lethality when homozygous.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi474 – 475DD → AA: Loss of activity. 1 Publication2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 48Chloroplast and mitochondrionSequence analysisAdd BLAST48
ChainiPRO_000035603849 – 572Proteinaceous RNase P 1, chloroplastic/mitochondrialAdd BLAST524

Proteomic databases

PaxDbiQ66GI4.
PRIDEiQ66GI4.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G32230.1.

Structurei

Secondary structure

1572
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi96 – 106Combined sources11
Turni107 – 109Combined sources3
Helixi113 – 126Combined sources14
Helixi132 – 142Combined sources11
Beta strandi149 – 152Combined sources4
Helixi155 – 169Combined sources15
Helixi176 – 189Combined sources14
Helixi192 – 204Combined sources13
Helixi211 – 223Combined sources13
Helixi227 – 239Combined sources13
Helixi246 – 258Combined sources13
Helixi262 – 275Combined sources14
Beta strandi277 – 279Combined sources3
Helixi281 – 292Combined sources12
Helixi294 – 297Combined sources4
Helixi306 – 316Combined sources11
Beta strandi331 – 336Combined sources6
Beta strandi342 – 344Combined sources3
Turni345 – 347Combined sources3
Helixi358 – 375Combined sources18
Helixi378 – 389Combined sources12
Beta strandi395 – 399Combined sources5
Helixi400 – 405Combined sources6
Helixi413 – 426Combined sources14
Beta strandi434 – 438Combined sources5
Helixi439 – 442Combined sources4
Helixi445 – 447Combined sources3
Helixi450 – 461Combined sources12
Beta strandi465 – 468Combined sources4
Helixi474 – 485Combined sources12
Beta strandi488 – 490Combined sources3
Helixi498 – 503Combined sources6
Turni504 – 506Combined sources3
Helixi507 – 515Combined sources9
Beta strandi516 – 522Combined sources7
Turni523 – 525Combined sources3
Beta strandi526 – 530Combined sources5
Beta strandi547 – 551Combined sources5
Turni556 – 558Combined sources3
Beta strandi563 – 568Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4G23X-ray1.98A77-572[»]
4G24X-ray1.95A77-572[»]
4G25X-ray2.00A77-572[»]
4G26X-ray1.75A77-572[»]
ProteinModelPortaliQ66GI4.
SMRiQ66GI4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati96 – 130PPR 1Add BLAST35
Repeati136 – 174PPR 2Add BLAST39
Repeati175 – 209PPR 3Add BLAST35
Repeati210 – 244PPR 4Add BLAST35

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi316 – 319Poly-Gly4

Sequence similaritiesi

Belongs to the PPR family. P subfamily.Curated
Contains 4 PPR (pentatricopeptide) repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transit peptide

Phylogenomic databases

eggNOGiKOG1347. Eukaryota.
COG0534. LUCA.
HOGENOMiHOG000006117.
InParanoidiQ66GI4.
KOiK18213.
OMAiKLDMCSR.
OrthoDBiEOG093605S6.
PhylomeDBiQ66GI4.

Family and domain databases

InterProiIPR002885. Pentatricopeptide_repeat.
IPR033443. PPR_long.
IPR031595. PRORP_C.
[Graphical view]
PfamiPF17177. PPR_long. 1 hit.
PF16953. PRORP. 1 hit.
[Graphical view]
PROSITEiPS51375. PPR. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q66GI4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRLTCFTPS FSRACCPLFA MMLKVPSVHL HHPRFSPFRF YHTSLLVKGT
60 70 80 90 100
RDRRLILVER SRHLCTLPLA AAKQSAASPS ENLSRKAKKK AIQQSPEALL
110 120 130 140 150
KQKLDMCSKK GDVLEALRLY DEARRNGVQL SQYHYNVLLY VCSLAEAATE
160 170 180 190 200
SSPNPGLSRG FDIFKQMIVD KVVPNEATFT NGARLAVAKD DPEMAFDMVK
210 220 230 240 250
QMKAFGIQPR LRSYGPALFG FCRKGDADKA YEVDAHMVES EVVPEEPELA
260 270 280 290 300
ALLKVSMDTK NADKVYKTLQ RLRDLVRQVS KSTFDMIEEW FKSEVATKTG
310 320 330 340 350
VKKWDVKKIR DAVVSGGGGW HGQGWLGTGK WNVKRTEMDE NGVCKCCKEK
360 370 380 390 400
LVCIDINPVE TETFAASLTR LACEREVKAN FNQFQEWLER HGPFDAVIDG
410 420 430 440 450
ANMGLVNQRS FSFFQLNNTV QRCQQISPSK RLPLVILHKS RVNGGPATYP
460 470 480 490 500
KNRALLEKWK NAGALYATPP GSNDDWYWLY AAVSCKCLLV TNDEMRDHLF
510 520 530 540 550
QLLGNSFFPR WKEKHQVRIS VTREDGLKLN MPPPYSIVIQ ESEDGTWHVP
560 570
MSVEDDLQTS RQWLCAKRSK TP
Length:572
Mass (Da):64,877
Last modified:October 11, 2004 - v1
Checksum:i80857002D71F42FA
GO

Sequence cautioni

The sequence AAD15382 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti250A → V in AAM13880 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC006223 Genomic DNA. Translation: AAD15382.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC08652.1.
AY091059 mRNA. Translation: AAM13880.1.
BT015418 mRNA. Translation: AAU05541.1.
PIRiE84730.
RefSeqiNP_850186.1. NM_179855.3.
UniGeneiAt.38106.

Genome annotation databases

EnsemblPlantsiAT2G32230.1; AT2G32230.1; AT2G32230.
GeneIDi817782.
GrameneiAT2G32230.1; AT2G32230.1; AT2G32230.
KEGGiath:AT2G32230.

Cross-referencesi

Web resourcesi

Arabidopsis PPR Protein Database

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC006223 Genomic DNA. Translation: AAD15382.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC08652.1.
AY091059 mRNA. Translation: AAM13880.1.
BT015418 mRNA. Translation: AAU05541.1.
PIRiE84730.
RefSeqiNP_850186.1. NM_179855.3.
UniGeneiAt.38106.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4G23X-ray1.98A77-572[»]
4G24X-ray1.95A77-572[»]
4G25X-ray2.00A77-572[»]
4G26X-ray1.75A77-572[»]
ProteinModelPortaliQ66GI4.
SMRiQ66GI4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT2G32230.1.

Proteomic databases

PaxDbiQ66GI4.
PRIDEiQ66GI4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G32230.1; AT2G32230.1; AT2G32230.
GeneIDi817782.
GrameneiAT2G32230.1; AT2G32230.1; AT2G32230.
KEGGiath:AT2G32230.

Organism-specific databases

TAIRiAT2G32230.

Phylogenomic databases

eggNOGiKOG1347. Eukaryota.
COG0534. LUCA.
HOGENOMiHOG000006117.
InParanoidiQ66GI4.
KOiK18213.
OMAiKLDMCSR.
OrthoDBiEOG093605S6.
PhylomeDBiQ66GI4.

Miscellaneous databases

PROiQ66GI4.

Family and domain databases

InterProiIPR002885. Pentatricopeptide_repeat.
IPR033443. PPR_long.
IPR031595. PRORP_C.
[Graphical view]
PfamiPF17177. PPR_long. 1 hit.
PF16953. PRORP. 1 hit.
[Graphical view]
PROSITEiPS51375. PPR. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPRRP1_ARATH
AccessioniPrimary (citable) accession number: Q66GI4
Secondary accession number(s): Q8RWW5, Q9SKX7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 16, 2008
Last sequence update: October 11, 2004
Last modified: November 30, 2016
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.