Q66GA8 (K6PF_YERPS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
6-phosphofructokinase
Short name=Phosphofructokinase
EC=2.7.1.11

Alternative name(s):
Phosphohexokinase

Gene names

Name:	pfkA
Ordered Locus Names:	YPTB0074

Organism

Yersinia pseudotuberculosis serotype I (strain IP32953) [Complete proteome] [HAMAP]

Taxonomic identifier

273123 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Yersinia › Yersinia pseudotuberculosis

Protein attributes

Sequence length	327 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. HAMAP-Rule MF_00339
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4. HAMAP-Rule MF_00339
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the phosphofructokinase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	fructose 6-phosphate metabolic process Inferred from electronic annotation. Source: InterPro glycolysis Inferred from electronic annotation. Source: HAMAP
Cellular_component	6-phosphofructokinase complex Inferred from electronic annotation. Source: InterPro
Molecular_function	6-phosphofructokinase activity Inferred from electronic annotation. Source: HAMAP ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 327	327	6-phosphofructokinase HAMAP-Rule MF_00339		PRO_1000059815
Regions
	Nucleotide binding	22 – 26	5	ATP By similarity
	Nucleotide binding	156 – 160	5	ATP By similarity
	Nucleotide binding	173 – 189	17	ATP By similarity
Sites
	Active site	129	1	Proton acceptor By similarity
	Metal binding	187	1	Magnesium; via carbonyl oxygen By similarity
	Metal binding	189	1	Magnesium By similarity
	Binding site	164	1	Substrate By similarity
	Binding site	245	1	Substrate By similarity
	Binding site	251	1	Substrate By similarity
	Binding site	254	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q66GA8 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 0F46B3A8AF0E430D	FASTA	327	35,411
	10 20 30 40 50 60 MVKKIGVLTS GGDAPGMNAA IRGVVRAALS AGLDVFGIED GYLGLYENRM KKLDRYSVSD 70 80 90 100 110 120 MINRGGTFLG SARFPEFRDP EVRKVALKNM HERGIDGLVV IGGDGSYAGA DLLTKEGGIH 130 140 150 160 170 180 CVGLPGTIDN DVAGTDYTIG FFTALETVVE AIDRLRDTSS SHQRISIVEV MGRYCGDLTL 190 200 210 220 230 240 AAAIAGGCEF IAIPEVEFKR DDLVAEIKAG IAKGKKHAIV AITEKLDDID SLAKYIEKET 250 260 270 280 290 300 GRETRGTVLG HIQRGGAPVA YDRILASRMG SYAVDLLLQD HDYKKGGFCV GVQNEKMVHE 310 320 LISVCIAPEN KKSKFKEDWY DTAKKLF

References

[1]

"Insights into the evolution of Yersinia pestis through whole-genome comparison with Yersinia pseudotuberculosis." Chain P.S.G., Carniel E., Larimer F.W., Lamerdin J., Stoutland P.O., Regala W.M., Georgescu A.M., Vergez L.M., Land M.L., Motin V.L., Brubaker R.R., Fowler J., Hinnebusch J., Marceau M., Medigue C., Simonet M., Chenal-Francisque V., Souza B. , Dacheux D., Elliott J.M., Derbise A., Hauser L.J., Garcia E. Proc. Natl. Acad. Sci. U.S.A. 101:13826-13831(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: IP32953.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX936398 Genomic DNA. Translation: CAH19314.1.
RefSeq	YP_068623.1. NC_006155.1.
3D structure databases
HSSP	HSSP built from PDB template 2PFK based on UniProtKB P0A796.
ProteinModelPortal	Q66GA8.
SMR	Q66GA8. Positions 1-327.
ModBase	Search...
Protein-protein interaction databases
STRING	273123.YPTB0074.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAH19314; CAH19314; YPTB0074.
GeneID	2956238.
KEGG	yps:YPTB0074.
PATRIC	18638188. VBIYerPse22266_0217.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0205.
HOGENOM	HOG000248870.
KO	K00850.
OMA	EFKTVEG.
ProtClustDB	PRK03202.
Enzyme and pathway databases
UniPathway	UPA00109; UER00182.
Family and domain databases
HAMAP	MF_00339. Phosphofructokinase.
InterPro	IPR012003. ATP_PFK_prok. IPR012828. PFKA_ATP. IPR022953. Phosphofructokinase. IPR015912. Phosphofructokinase_CS. IPR000023. Phosphofructokinase_dom. [Graphical view]
Pfam	PF00365. PFK. 1 hit. [Graphical view]
PIRSF	PIRSF000532. ATP_PFK_prok. 1 hit.
PRINTS	PR00476. PHFRCTKINASE.
SUPFAM	SSF53784. Ppfruckinase. 1 hit.
TIGRFAMs	TIGR02482. PFKA_ATP. 1 hit.
PROSITE	PS00433. PHOSPHOFRUCTOKINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

K6PF_YERPS

Accession

Primary (citable) accession number: Q66GA8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	October 11, 2004
Last modified:	May 1, 2013
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program