Reviewed, UniProtKB/Swiss-Prot Q66ED2 (CYSH_YERPS)

Names and origin

Protein names

Recommended name:
    Phosphoadenosine phosphosulfate reductase
    EC=1.8.4.8
Alternative name(s):
    PAPS reductase, thioredoxin dependent
    PAdoPS reductase
    3'-phosphoadenylylsulfate reductase
    PAPS sulfotransferase

Gene names

Name:	cysH
Ordered Locus Names:	YPTB0761

Organism

Yersinia pseudotuberculosis [Complete proteome] [HAMAP]

Taxonomic identifier

633 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Yersinia

Protein attributes

Sequence length	244 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Reduction of activated sulfate into sulfite. HAMAP MF_00063
Catalytic activity	Adenosine 3',5'-bisphosphate + sulfite + thioredoxin disulfide = 3'-phosphoadenylyl sulfate + thioredoxin. HAMAP MF_00063
Pathway	Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 3/3. HAMAP MF_00063
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the PAPS reductase family. CysH subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteine biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW sulfate assimilation, phosphoadenylyl sulfate reduction by phosphoadenylyl-sulfate reductase (thioredoxin) Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	phosphoadenylyl-sulfate reductase (thioredoxin) activity Inferred from electronic annotation. Source: HAMAP transferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 244	244	Phosphoadenosine phosphosulfate reductase HAMAP MF_00063		PRO_1000008948

Sequences

	Sequence		Length	Mass (Da)
	Q66ED2-1 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: D9C60C79D63593DF	FASTA	244	27,852
	10 20 30 40 50 60 MSQFNLSELN ALPKAKQAAA LVLVNGQLEH LTAQERVSWA LDNLPGEFVL SSSFGIQAAV 70 80 90 100 110 120 CLHLVTRQRP DIPVILTDTG YLFPETYRFI DDLTEKLQLN LQVFRAAHSP AWQEARYGKL 130 140 150 160 170 180 WEQGVEGIER YNTLNKVEPM NRALEALGAQ TWFAGLRREQ SGGRSQLPVL ALQRGIFKLL 190 200 210 220 230 240 PIIDWDNRQV YQYLTQHGLS YHPLWEQGYL SVGDTHTTRK WEPGMSEEET RFFGLKRECG LHEG

References

[1]

"Insights into the evolution of Yersinia pestis through whole-genome comparison with Yersinia pseudotuberculosis." Chain P.S.G., Carniel E., Larimer F.W., Lamerdin J., Stoutland P.O., Regala W.M., Georgescu A.M., Vergez L.M., Land M.L., Motin V.L., Brubaker R.R., Fowler J., Hinnebusch J., Marceau M., Medigue C., Simonet M., Chenal-Francisque V., Souza B. , Dacheux D., Elliott J.M., Derbise A., Hauser L.J., Garcia E. Proc. Natl. Acad. Sci. U.S.A. 101:13826-13831(2004) [PubMed: 15358858] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: IP32953 / Serotype I.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX936398 Genomic DNA. Translation: CAH20001.1.
RefSeq	YP_069302.1.
3D structure databases
SMR	Q66ED2. Positions 2-230.
ModBase	Search...
Genome annotation databases
GeneID	2955405.
GenomeReviews	Gene locus YPTB0761 in contig BX936398_GR.
KEGG	yps:YPTB0761.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q66ED2.
OMA	Q66ED2. AWTDEDM.
Enzyme and pathway databases
BioCyc	YPSE273123:YPTB0761-MON.
BRENDA	1.8.4.8. 20871.
Family and domain databases
HAMAP	MF_00063. [Tree]
InterPro	IPR004511. PAdo_PSO4_Rdtase_CysH. IPR002500. PAPS_reduct. IPR011800. PAPS_reductase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF01507. PAPS_reduct. 1 hit. [Graphical view]
TIGRFAMs	TIGR00434. cysH. 1 hit. TIGR02057. PAPS_reductase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CYSH_YERPS

Accession

Primary (citable) accession number: Q66ED2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 11, 2004
Last modified:	June 16, 2009
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)