Reviewed, UniProtKB/Swiss-Prot Q66DP5 (ASCD_YERPS)

Names and origin

Protein names

Recommended name:
    CDP-6-deoxy-L-threo-D-glycero-4-hexulose-3-dehydrase reductase
    EC=1.17.1.-
Alternative name(s):
    CDP-6-deoxy-delta-3,4-glucoseen reductase
      Short name=E3

Gene names

Name:	ascD
Synonyms:	ddhD, rfbI
Ordered Locus Names:	YPTB0998

Organism

Yersinia pseudotuberculosis [Complete proteome] [HAMAP]

Taxonomic identifier

633 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Yersinia

Protein attributes

Sequence length	329 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Participates in the conversion of CDP-6-deoxy-D-glycero-L-threo-4-hexulose to 3,6-dideoxy-D-glycero-D-glycero-4-hexulose together with CDP-6-deoxy-D-glycero-L-threo-4-hexulose-3-dehydrase (E1) in two consecutive steps. The detailed mechanism of E3 is not yet resolved.
Pathway	Nucleotide-sugar biosynthesis; CDP-ascarylose biosynthesis. Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis.
Subunit structure	Monomer.
Sequence similarities	Contains 1 2Fe-2S ferredoxin-type domain. Contains 1 FAD-binding FR-type domain.

Ontologies

Keywords
Biological process	Electron transport Transport
Ligand	2Fe-2S Iron Iron-sulfur Metal-binding NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW oxidoreductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 329	328	CDP-6-deoxy-L-threo-D-glycero-4-hexulose-3-dehydrase reductase		PRO_0000189394
Regions
	Domain	2 – 93	92	2Fe-2S ferredoxin-type
	Domain	98 – 197	100	FAD-binding FR-type
Sites
	Metal binding	37	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	42	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	45	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	75	1	Iron-sulfur (2Fe-2S) By similarity
Experimental info
	Sequence conflict	111	1	V → I Ref.1 Ref.2 Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q66DP5-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 8B1B98EE473704FF	FASTA	329	36,033
	10 20 30 40 50 60 MSLNVKLHPS GIIFTSDGTS TILDAALDSN IHIEYSCKDG TCGSCKAILI SGEVDSAENT 70 80 90 100 110 120 FLTEEDVAKG AILTCCSKAK SDIELDVNYY PELSHIQKKT YPCKLDSIEF VGEDIAILSL 130 140 150 160 170 180 RLPPTAKIQY LAGQYIDLII NGQRRSYSIA NAPGGNGNIE LHVRKVVNGV FSNIIFNELK 190 200 210 220 230 240 LQQLLRIEGP QGTFFVREDN LPIVFLAGGT GFAPVKSMVE ALINKNDQRQ VHIYWGMPAG 250 260 270 280 290 300 HNFYSDIANE WAIKHPNIHY VPVVSGDDST WTGATGFVHQ AVLEDIPDLS LFNVYACGSL 310 320 AMITAARNDF INHGLAENKF FSDAFVPSK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"CDP-6-deoxy-delta 3,4-glucoseen reductase from Yersinia pseudotuberculosis: enzyme purification and characterization of the cloned gene." Lo S.F., Miller V.P., Lei Y., Thorson J.S., Liu H.-W., Schottel J.L. J. Bacteriol. 176:460-468(1994) [PubMed: 8288541] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE. Strain: V.
[2]	"Molecular analysis of the 3,6-dideoxyhexose pathway genes of Yersinia pseudotuberculosis serogroup IIA." Kessler A.C., Haase A., Reeves P.R. J. Bacteriol. 175:1412-1422(1993) [PubMed: 8444803] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: M85 / Serotype IIA.
[3]	"Studies of the biosynthesis of 3,6-dideoxyhexoses: molecular cloning and characterization of the asc (ascarylose) region from Yersinia pseudotuberculosis serogroup VA." Thorson J.S., Lo S.F., Ploux O., He X., Liu H.-W. J. Bacteriol. 176:5483-5493(1994) [PubMed: 8071227] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Serotype VA.
[4]	Pacinelli E., Wang L., Reeves P.R. Submitted (DEC-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Serotype IVB.
[5]	"Insights into the evolution of Yersinia pestis through whole-genome comparison with Yersinia pseudotuberculosis." Chain P.S.G., Carniel E., Larimer F.W., Lamerdin J., Stoutland P.O., Regala W.M., Georgescu A.M., Vergez L.M., Land M.L., Motin V.L., Brubaker R.R., Fowler J., Hinnebusch J., Marceau M., Medigue C., Simonet M., Chenal-Francisque V., Souza B. , Dacheux D., Elliott J.M., Derbise A., Hauser L.J., Garcia E. Proc. Natl. Acad. Sci. U.S.A. 101:13826-13831(2004) [PubMed: 15358858] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: IP32953 / Serotype I.
[6]	"Kinetic characterization of an organic radical in the ascarylose biosynthetic pathway." Johnson D.A., Gassner G.T., Bandarian V., Ruzicka F.J., Ballou D.P., Reed G.H., Liu H.-W. Biochemistry 35:15846-15856(1996) [PubMed: 8961949] [Abstract] Cited for: CHARACTERIZATION.
[7]	"Kinetics of the reductive half-reaction of the iron-sulfur flavoenzyme CDP-6-deoxy-L-threo-D-glycero-4-hexulose-3-dehydrase reductase." Gassner G.T., Johnson D.A., Liu H.-W., Ballou D.P. Biochemistry 35:7752-7761(1996) [PubMed: 8672475] [Abstract] Cited for: CHARACTERIZATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L25594 Unassigned DNA. Translation: AAA16760.1. AF461770 Genomic DNA. Translation: AAB49398.1. L33181 Genomic DNA. Translation: AAA88698.1. AF461769 Genomic DNA. Translation: AAN23052.1. BX936398 Genomic DNA. Translation: CAH20238.1.
PIR	A36952.
RefSeq	YP_069539.1.
3D structure databases
HSSP	HSSP built from PDB template 1QFJ based on UniProtKB P23486.
ModBase	Search...
Genome annotation databases
GeneID	2955441.
GenomeReviews	Gene locus YPTB0998 in contig BX936398_GR.
KEGG	yps:YPTB0998.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q66DP5.
Enzyme and pathway databases
BioCyc	MetaCyc:MON-13782. YPSE273123:YPTB0998-MON.
Family and domain databases
InterPro	IPR006058. 2Fe2S_fd_BS. IPR012675. b-grasp_ferredoxin-like. IPR001041. Ferredoxin. IPR008333. OxRdtase_FAD-bd. IPR001433. OxRdtase_FAD/NAD_bd. IPR001221. Phe_hydroxylase. [Graphical view]
Gene3D	G3DSA:3.10.20.30. Ferredoxin_fold. 1 hit.
Pfam	PF00970. FAD_binding_6. 1 hit. PF00111. Fer2. 1 hit. PF00175. NAD_binding_1. 1 hit. [Graphical view]
PRINTS	PR00410. PHEHYDRXLASE.
PROSITE	PS00197. 2FE2S_FER_1. 1 hit. PS51085. 2FE2S_FER_2. 1 hit. PS51384. FAD_FR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASCD_YERPS

Accession

Primary (citable) accession number: Q66DP5
Secondary accession number(s): P37911, Q8GJ93

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 7, 2004
Last sequence update:	January 23, 2007
Last modified:	November 25, 2008
This is version 36 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)