Q66DC5 (SYQ_YERPS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamine--tRNA ligase
EC=6.1.1.18

Alternative name(s):
Glutaminyl-tRNA synthetase
Short name=GlnRS

Gene names

Name:	glnS
Ordered Locus Names:	YPTB1121

Organism

Yersinia pseudotuberculosis serotype I (strain IP32953) [Complete proteome] [HAMAP]

Taxonomic identifier

273123 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Yersinia › Yersinia pseudotuberculosis

Protein attributes

Sequence length	555 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln). HAMAP-Rule MF_00126
Subunit structure	Monomer By similarity. HAMAP-Rule MF_00126
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00126.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glutaminyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 555	555	Glutamine--tRNA ligase HAMAP-Rule MF_00126		PRO_0000242884
Regions
	Motif	34 – 44	11	"HIGH" region HAMAP-Rule MF_00126
	Motif	268 – 272	5	"KMSKS" region HAMAP-Rule MF_00126
Sites
	Binding site	271	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q66DC5 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: AD3C68D6FB56C0B5	FASTA	555	63,751
	10 20 30 40 50 60 MSEAEARPSN FIRQIIDEDL ASGKHTSVHT RFPPEPNGYL HIGHAKSICL NFGIAEDYQG 70 80 90 100 110 120 QCNLRFDDTN PVKEDVEFVE SIKRDVEWLG FTWSGDVRYS SDYFDQLYQY AVELINKGLA 130 140 150 160 170 180 YVDELTPEQM REYRGTLTAP GKNSPYRDRS VEENLALFEK MRAGGFAEGT ACLRAKIDMA 190 200 210 220 230 240 SPFIVMRDPV LYRIKFAEHH QSGNKWCIYP MYDFTHCISD ALEGITHSLC TLEFQDNRRL 250 260 270 280 290 300 YDWVLDNISI DCHPRQYEFS RLNLEYTIMS KRKLNQLVTE KVVEGWDDPR MPTISGLRRR 310 320 330 340 350 360 GYTAASIREF CRRIGVTKQD NNVEMMSLES CIRDDLNEHA PRAMAVLDPI KVVIENRAAG 370 380 390 400 410 420 EEWLTMPNHP NNPEMGSRQV PFDSEIYIDR ADFREEANKQ YKRLVLGKEV RLRNAYVIKA 430 440 450 460 470 480 ERVEKDAEGN VTTLYCSYDA ETLNKDPADG RKVKGVIHWV SVAHALPAEI RLYDRLFNVP 490 500 510 520 530 540 NPAAAEDFLS TINPESLVIR QGFVEPSLAD AVSDKTYQFE REGYFCADSR YSRPGALVFN 550 RTVGLRDTWA AKATQ

References

[1]

"Insights into the evolution of Yersinia pestis through whole-genome comparison with Yersinia pseudotuberculosis." Chain P.S.G., Carniel E., Larimer F.W., Lamerdin J., Stoutland P.O., Regala W.M., Georgescu A.M., Vergez L.M., Land M.L., Motin V.L., Brubaker R.R., Fowler J., Hinnebusch J., Marceau M., Medigue C., Simonet M., Chenal-Francisque V., Souza B. , Dacheux D., Elliott J.M., Derbise A., Hauser L.J., Garcia E. Proc. Natl. Acad. Sci. U.S.A. 101:13826-13831(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: IP32953.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX936398 Genomic DNA. Translation: CAH20361.1.
RefSeq	YP_069659.1. NC_006155.1.
3D structure databases
ProteinModelPortal	Q66DC5.
SMR	Q66DC5. Positions 9-548.
ModBase	Search...
Protein-protein interaction databases
STRING	273123.YPTB1121.
Proteomic databases
PRIDE	Q66DC5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAH20361; CAH20361; YPTB1121.
GeneID	2955703.
KEGG	yps:YPTB1121.
PATRIC	18640692. VBIYerPse22266_1423.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HOG000259232.
KO	K01886.
OMA	RMPTIAG.
ProtClustDB	PRK05347.
Enzyme and pathway databases
BioCyc	YPSE273123:GI1M-1180-MONOMER.
Family and domain databases
Gene3D	1.10.1160.10. 1 hit. 2.40.240.10. 2 hits. 3.40.50.620. 2 hits.
HAMAP	MF_00126. Gln_tRNA_synth.
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR004514. Gln-tRNA-synth_Ib. IPR022861. Gln_tRNA_ligase_bac. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd. IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl. IPR011035. Ribosomal_L25/Gln-tRNA_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
PANTHER	PTHR10119. PTHR10119. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. PF03950. tRNA-synt_1c_C. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF50715. Ribosomal_L25rel. 1 hit.
TIGRFAMs	TIGR00440. glnS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYQ_YERPS

Accession

Primary (citable) accession number: Q66DC5

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2006
Last sequence update:	October 11, 2004
Last modified:	May 29, 2013
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program