Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase

Gene

arnT

Organism
Yersinia pseudotuberculosis serotype I (strain IP32953)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the transfer of the L-Ara4N moiety of the glycolipid undecaprenyl phosphate-alpha-L-Ara4N to lipid A. The modified arabinose is attached to lipid A and is required for resistance to polymyxin and cationic antimicrobial peptides (By similarity).By similarity

Catalytic activityi

4-amino-4-deoxy-alpha-L-arabinopyranosyl di-trans,octa-cis-undecaprenyl phosphate + lipid IV(A) = lipid II(A) + di-trans,octa-cis-undecaprenyl phosphate.

Pathwayi: 4-amino-4-deoxy-beta-L-arabinose-lipid A biosynthesis

This protein is involved in the pathway 4-amino-4-deoxy-beta-L-arabinose-lipid A biosynthesis, which is part of Lipopolysaccharide metabolism.
View all proteins of this organism that are known to be involved in the pathway 4-amino-4-deoxy-beta-L-arabinose-lipid A biosynthesis and in Lipopolysaccharide metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Lipid A biosynthesis, Lipid biosynthesis, Lipid metabolism, Lipopolysaccharide biosynthesis

Enzyme and pathway databases

BioCyciYPSE273123:GI1M-2412-MONOMER.
UniPathwayiUPA00037.

Protein family/group databases

CAZyiGT83. Glycosyltransferase Family 83.

Names & Taxonomyi

Protein namesi
Recommended name:
Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase (EC:2.4.2.43)
Alternative name(s):
4-amino-4-deoxy-L-arabinose lipid A transferase
Lipid IV(A) 4-amino-4-deoxy-L-arabinosyltransferase
Polymyxin resistance protein PmrK
Undecaprenyl phosphate-alpha-L-Ara4N transferase
Gene namesi
Name:arnT
Synonyms:pmrK
Ordered Locus Names:YPTB2326
OrganismiYersinia pseudotuberculosis serotype I (strain IP32953)
Taxonomic identifieri273123 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeYersinia
Proteomesi
  • UP000001011 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei87 – 10721HelicalSequence analysisAdd
BLAST
Transmembranei115 – 13521HelicalSequence analysisAdd
BLAST
Transmembranei178 – 19821HelicalSequence analysisAdd
BLAST
Transmembranei206 – 22621HelicalSequence analysisAdd
BLAST
Transmembranei262 – 28221HelicalSequence analysisAdd
BLAST
Transmembranei293 – 31321HelicalSequence analysisAdd
BLAST
Transmembranei315 – 33521HelicalSequence analysisAdd
BLAST
Transmembranei351 – 37121HelicalSequence analysisAdd
BLAST
Transmembranei384 – 40421HelicalSequence analysisAdd
BLAST
Transmembranei414 – 43421HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 554554Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferasePRO_0000121516Add
BLAST

Expressioni

Inductioni

Activated by low magnesium concentrations, via the two-component regulatory system PhoP/PhoQ.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ66A06.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 83 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000273002.
KOiK07264.
OMAiWMALRRT.

Family and domain databases

HAMAPiMF_01165. ArnT_transfer. 1 hit.
InterProiIPR022839. ArnT_tfrase.
IPR003342. Glyco_trans_39/83.
[Graphical view]
PfamiPF02366. PMT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q66A06-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLLKDSGAA LLALFFVLVY LLPVNSRLLW QPDETRYAEI SREMLQRGDW
60 70 80 90 100
VVPYFMDIRY FEKPVAGYWF NNISQWIFGD SNFAVRFGSI FSTALSAVLV
110 120 130 140 150
YWLATLLWRN RSTSVLATLI YLSFLLVFGI GTYAVLDPMI SLWLTAAMVS
160 170 180 190 200
FYLTLKAENW QQKVGAYALL GVACGMGFMT KGFLALAVPV IAVLPIVIQQ
210 220 230 240 250
KRIKDLVVFG PIAIVCAVLL SLPWALAIAQ REPDFWNYFF WVEHIQRFAE
260 270 280 290 300
ASAQHKSPIW YYLPILCIGV LPWLGLLPGA LFKGWRERAT KPELFFLLSW
310 320 330 340 350
VVMPLLFFSV AKGKLPTYIL PCMAPLSLLM AAYATDCANN IRMRALKING
360 370 380 390 400
VINLLFGVAC ALVIVVIGLG LVKDIVAYGP QENQKVWLGV LAFAGWGVTG
410 420 430 440 450
FITLRNNARN WRWAAACPLL FILLVGYLIP QQVVDSKQPQ NFIKNNFSEL
460 470 480 490 500
SSSRYVLTDS VGVAAGLAWE LKRSDILMFS EKGELTYGLA YPDSQDNYIS
510 520 530 540 550
NDDFPTWLAQ ARKEGDVSLV VQLAKNEALP AHLPPADKVN LMNRLALLWY

QKTP
Length:554
Mass (Da):62,289
Last modified:October 11, 2004 - v1
Checksum:i11BFF54C66FBDB48
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti514 – 5141E → K in AAK69644 (PubMed:15583148).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF336802 Genomic DNA. Translation: AAK69644.1.
BX936398 Genomic DNA. Translation: CAH21564.1.
RefSeqiWP_002211821.1. NZ_CP009712.1.

Genome annotation databases

EnsemblBacteriaiCAH21564; CAH21564; YPTB2326.
KEGGiypo:BZ17_128.
yps:YPTB2326.
PATRICi18643568. VBIYerPse22266_2842.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF336802 Genomic DNA. Translation: AAK69644.1.
BX936398 Genomic DNA. Translation: CAH21564.1.
RefSeqiWP_002211821.1. NZ_CP009712.1.

3D structure databases

ProteinModelPortaliQ66A06.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGT83. Glycosyltransferase Family 83.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAH21564; CAH21564; YPTB2326.
KEGGiypo:BZ17_128.
yps:YPTB2326.
PATRICi18643568. VBIYerPse22266_2842.

Phylogenomic databases

HOGENOMiHOG000273002.
KOiK07264.
OMAiWMALRRT.

Enzyme and pathway databases

UniPathwayiUPA00037.
BioCyciYPSE273123:GI1M-2412-MONOMER.

Family and domain databases

HAMAPiMF_01165. ArnT_transfer. 1 hit.
InterProiIPR022839. ArnT_tfrase.
IPR003342. Glyco_trans_39/83.
[Graphical view]
PfamiPF02366. PMT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiARNT_YERPS
AccessioniPrimary (citable) accession number: Q66A06
Secondary accession number(s): Q93PD6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: October 11, 2004
Last modified: September 7, 2016
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.