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Protein

Dihydroorotase

Gene

pyrC

Organism
Yersinia pseudotuberculosis serotype I (strain IP32953)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reversible cyclization of carbamoyl aspartate to dihydroorotate.UniRule annotation

Catalytic activityi

(S)-dihydroorotate + H2O = N-carbamoyl-L-aspartate.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 2 Zn2+ ions per subunit.UniRule annotation

Pathwayi: UMP biosynthesis via de novo pathway

This protein is involved in step 3 of the subpathway that synthesizes (S)-dihydroorotate from bicarbonate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Carbamoyl-phosphate synthase small chain (carA), Carbamoyl-phosphate synthase large chain (carB)
  2. Aspartate carbamoyltransferase (pyrB)
  3. Dihydroorotase (pyrC)
This subpathway is part of the pathway UMP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-dihydroorotate from bicarbonate, the pathway UMP biosynthesis via de novo pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi17Zinc 1; via tele nitrogenUniRule annotation1
Metal bindingi19Zinc 1; via tele nitrogenUniRule annotation1
Binding sitei45SubstrateUniRule annotation1
Metal bindingi103Zinc 1; via carbamate groupUniRule annotation1
Metal bindingi103Zinc 2; via carbamate groupUniRule annotation1
Metal bindingi140Zinc 2; via pros nitrogenUniRule annotation1
Binding sitei140SubstrateUniRule annotation1
Metal bindingi178Zinc 2; via tele nitrogenUniRule annotation1
Binding sitei223Substrate; via amide nitrogen and carbonyl oxygenUniRule annotation1
Active sitei251UniRule annotation1
Metal bindingi251Zinc 1UniRule annotation1
Binding sitei255SubstrateUniRule annotation1
Binding sitei267Substrate; via carbonyl oxygenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processPyrimidine biosynthesis
LigandMetal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00070; UER00117.

Names & Taxonomyi

Protein namesi
Recommended name:
DihydroorotaseUniRule annotation (EC:3.5.2.3UniRule annotation)
Short name:
DHOaseUniRule annotation
Gene namesi
Name:pyrCUniRule annotation
Ordered Locus Names:YPTB2482
OrganismiYersinia pseudotuberculosis serotype I (strain IP32953)
Taxonomic identifieri273123 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesYersiniaceaeYersinia
Proteomesi
  • UP000001011 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000240771 – 348DihydroorotaseAdd BLAST348

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei103N6-carboxylysineUniRule annotation1

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ669K2.
SMRiQ669K2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni19 – 21Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the metallo-dependent hydrolases superfamily. DHOase family. Class II DHOase subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000256259.
KOiK01465.
OMAiHLRDGAM.

Family and domain databases

CDDicd01294. DHOase. 1 hit.
HAMAPiMF_00219. PyrC_classII. 1 hit.
InterProiView protein in InterPro
IPR006680. Amidohydro-rel.
IPR004721. DHOdimr.
IPR002195. Dihydroorotase_CS.
IPR032466. Metal_Hydrolase.
PANTHERiPTHR43137:SF1. PTHR43137:SF1. 1 hit.
PfamiView protein in Pfam
PF01979. Amidohydro_1. 1 hit.
PIRSFiPIRSF001237. DHOdimr. 1 hit.
SFLDiSFLDF00074. dihydroorotase3. 1 hit.
SUPFAMiSSF51556. SSF51556. 1 hit.
TIGRFAMsiTIGR00856. pyrC_dimer. 1 hit.
PROSITEiView protein in PROSITE
PS00483. DIHYDROOROTASE_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q669K2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTAQPQTLKI RRPDDWHIHL RDDEMLSTVL PYTSEVFARA IVMPNLAQPI
60 70 80 90 100
TTVASAIAYR ERILAAVPAG HKFTPLMTCY LTNSLDVKEL TTGFEQGVFT
110 120 130 140 150
AAKLYPANAT TNSTHGVSDI PAIYPLFEQM QKIGMPLLIH GEVTDAAVDI
160 170 180 190 200
FDREARFIDQ ILEPIRQKFP ELKIVFEHIT TKDAADYVLA GNRFLGATVT
210 220 230 240 250
PQHLMFNRNH MLVGGIRPHL FCLPILKRST HQQALRAAVA SGSDRFFLGT
260 270 280 290 300
DSAPHAKHRK ESSCGCAGVF NAPAALPAYA SVFEELNALQ HLEAFCALNG
310 320 330 340
PRFYGLPVND DVVELVRTPF LQPEEIPLGN ESVIPFLAGQ TLNWSVKR
Length:348
Mass (Da):38,570
Last modified:October 11, 2004 - v1
Checksum:iB92B613E026ED571
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX936398 Genomic DNA. Translation: CAH21720.1.
RefSeqiWP_011192610.1. NZ_CP009712.1.

Genome annotation databases

EnsemblBacteriaiCAH21720; CAH21720; YPTB2482.
KEGGiypo:BZ17_4155.
yps:YPTB2482.
PATRICifig|273123.14.peg.4377.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiPYRC_YERPS
AccessioniPrimary (citable) accession number: Q669K2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 11, 2004
Last modified: July 5, 2017
This is version 82 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families