Q669C8 (MENH_YERPS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase
Short name=SHCHC synthase
EC=4.2.99.20

Gene names

Name:	menH
Ordered Locus Names:	YPTB2559

Organism

Yersinia pseudotuberculosis serotype I (strain IP32953) [Complete proteome] [HAMAP]

Taxonomic identifier

273123 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Yersinia › Yersinia pseudotuberculosis

Protein attributes

Sequence length	272 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes a proton abstraction reaction that results in 2,5-elimination of pyruvate from 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) and the formation of 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate (SHCHC). Is also able to catalyze the hydrolysis of the thioester bond in palmitoyl-CoA in vitro By similarity. HAMAP-Rule MF_01660
Catalytic activity	5-enolpyruvoyl-6-hydroxy-2-succinylcyclohex-3-ene-1-carboxylate = (1R,6R)-6-hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylate + pyruvate. HAMAP-Rule MF_01660
Pathway	Cofactor biosynthesis; menaquinone biosynthesis; menaquinone-2 from chorismate: step 3/8. HAMAP-Rule MF_01660
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the AB hydrolase superfamily. MenH family.

Ontologies

Keywords
Biological process	Menaquinone biosynthesis
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	menaquinone biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 272	272	2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase HAMAP-Rule MF_01660		PRO_0000341934

Sequences

	Sequence		Length	Mass (Da)
	Q669C8 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 33CE37CF96FC0D21	FASTA	272	30,258
	10 20 30 40 50 60 MTTLACQKLA PHPESPRHQH AGPWLVWLHG LLGSGQDWLP VAQLCGDYPS LLIDLPGHGQ 70 80 90 100 110 120 SVSLSADGFA DISRQLSQTL QANGIREYWL AGYSLGGRIA IYHACYGRHH GLQGLLVEGG 130 140 150 160 170 180 NLGLENAELR QARLQQDRQW AQRFRQEPLP QVLDDWYQQA VFADLDPQQR EQLVLLRADN 190 200 210 220 230 240 HGTAVAEMLE ATSLGHQPWL LPALQRLNVP YTYLCGDRDH KFLQLAQQYR LPLHTLARAG 250 260 270 HNAHRANPGA FAAQVLAFLS QSSCLPPSSL SR

References

[1]

"Insights into the evolution of Yersinia pestis through whole-genome comparison with Yersinia pseudotuberculosis." Chain P.S.G., Carniel E., Larimer F.W., Lamerdin J., Stoutland P.O., Regala W.M., Georgescu A.M., Vergez L.M., Land M.L., Motin V.L., Brubaker R.R., Fowler J., Hinnebusch J., Marceau M., Medigue C., Simonet M., Chenal-Francisque V., Souza B. , Dacheux D., Elliott J.M., Derbise A., Hauser L.J., Garcia E. Proc. Natl. Acad. Sci. U.S.A. 101:13826-13831(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: IP32953.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX936398 Genomic DNA. Translation: CAH21797.1.
RefSeq	YP_071069.1. NC_006155.1.
3D structure databases
ProteinModelPortal	Q669C8.
ModBase	Search...
Protein-protein interaction databases
STRING	273123.YPTB2559.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAH21797; CAH21797; YPTB2559.
GeneID	2954333.
KEGG	yps:YPTB2559.
PATRIC	18644119. VBIYerPse22266_3117.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0596.
HOGENOM	HOG000028072.
KO	K08680.
OMA	LGGRIAM.
ProtClustDB	PRK11126.
Enzyme and pathway databases
UniPathway	UPA00079; UER00900.
Family and domain databases
HAMAP	MF_01660. MenH.
InterPro	IPR022485. SHCHC_synthase_MenH. [Graphical view]
TIGRFAMs	TIGR03695. menH_SHCHC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MENH_YERPS

Accession

Primary (citable) accession number: Q669C8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 2008
Last sequence update:	October 11, 2004
Last modified:	May 1, 2013
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program