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Q66915

- CAPSD_FCVUR

UniProt

Q66915 - CAPSD_FCVUR

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Protein

Capsid protein

Gene

ORF2

Organism
Feline calicivirus (strain Cat/United States/Urbana/1960) (FCV)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Capsid protein self assembles to form an icosahedral capsid with a T=3 symmetry, about 38 nm in diameter, and consisting of 180 capsid proteins. A smaller form of capsid with a diameter of 23 nm might be capsid proteins assembled as icosahedron with T=1 symmetry. The capsid encapsulate the genomic RNA and VP2 proteins. Attaches virion to target cells by binding to feline junctional adhesion molecule A (F11R) and/or to alpha-2,6-linked sialic acid. Once attached, the virion is endocytosed. Acidification of the endosome induces conformational change of capsid protein thereby injecting virus genomic RNA into host cytoplasm (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei124 – 1252Cleavage; by calicivirin

Names & Taxonomyi

Protein namesi
Recommended name:
Capsid protein
Alternative name(s):
Coat protein
Short name:
CP
Cleaved into the following chain:
Gene namesi
ORF Names:ORF2
OrganismiFeline calicivirus (strain Cat/United States/Urbana/1960) (FCV)
Taxonomic identifieri292349 [NCBI]
Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageCaliciviridaeVesivirus
Virus hostiFelis catus (Cat) (Felis silvestris catus) [TaxID: 9685]
ProteomesiUP000001098: Genome

Subcellular locationi

GO - Cellular componenti

  1. host cell cytoplasm Source: UniProtKB-KW
  2. T=3 icosahedral viral capsid Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Host cytoplasm, T=3 icosahedral capsid protein, Virion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi124 – 1241E → H, K or L: Complete loss of precursor cleavage by viral calcivirin. 1 Publication
Mutagenesisi124 – 1241E → Q or D: Partial loss of precursor cleavage by calcivirin. 1 Publication
Mutagenesisi125 – 1251A → G, H, L, R or V: No effect on precursor cleavage by calcivirin. 1 Publication
Mutagenesisi125 – 1251A → P: Complete loss of precursor cleavage by viral calcivirin. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 124124By similarityPRO_0000036880Add
BLAST
Chaini125 – 668544Capsid proteinBy similarityPRO_0000036881Add
BLAST
Chaini?465 – 668204Protein 40kPRO_0000341625Add
BLAST

Post-translational modificationi

Cleaved by virus calcivirin to produce mature capsid protein.
Cleaved by host caspase-2 and caspase-6 to generate protein p40.By similarity

Interactioni

Subunit structurei

Homodimerizes, then multimerizes. May bind to VP3 and Vpg proteins. Binds to alpha-2,6-linked sialic acid at surface of target cells. Interacts with host F11R (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ66915.
SMRiQ66915. Positions 129-662.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di2.60.120.20. 1 hit.
InterProiIPR004005. Calicivirus_coat.
IPR029053. Viral_coat.
[Graphical view]
PfamiPF00915. Calici_coat. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q66915-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MCSTCANVLK YYNWDPHFKL VINPNKFLSI GFCDNPLMCC YPELLPEFGT
60 70 80 90 100
VWDCDQSPLQ IYLESILGDD EWSSTYEAID PVVPPMHWNE AGKIFQPHPG
110 120 130 140 150
VLMHHIIGEV AKAWDPNLPL FRLEADDGSI TAPEQGTVVG GVIAEPSSQM
160 170 180 190 200
STAADMASGK SVDSEWEAFF SFHTSVNWST SETQGKILFK QSLGPLLNPY
210 220 230 240 250
LEHLSKLYVA WSGSVEVRFS ISGSGVFGGK LAAIVVPPGV DPIQSTSMLQ
260 270 280 290 300
YPHVLFDARQ VEPVIFTIPD LRSTLYHLMS DTDTTSLVIM VYNDLINPYA
310 320 330 340 350
NDSNSSGCIV TVETKPGSDF KFHLLKPPGS MLTHGSVPSD LIPKTSSLWI
360 370 380 390 400
GNRFWSDITD FVIRPFVFQA NRHFDFNQET AGWSTPRFRP ITVTISEKNG
410 420 430 440 450
AKLGVGVATD FIVPGIPDGW PDTTIGEKLV PAGDYAITNG SGNDITTANQ
460 470 480 490 500
YDAADIIRNN TNFKGMYICG SLQRAWGDKK ISNTAFITTA TVEGNDLIPS
510 520 530 540 550
NVIDQTKIAI FQDNHVQDEV QTSDDTLALL GYTGIGEEAI GANRERVVRI
560 570 580 590 600
STLPETGARG GNHPIFYKNS IKLGYVIRSI DVFNSQILHT SRQLSLNHYL
610 620 630 640 650
LPPDSFAVYR IIDSNGSWFD VGIDFDGFSF VGVSDVGKLE FPLTASYMGI
660
QLAKIRLASN IRSTMTKL
Length:668
Mass (Da):73,518
Last modified:November 1, 1996 - v1
Checksum:iC1E38D92BB6E5FA6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L40021 Genomic RNA. Translation: AAA79324.1.
RefSeqiNP_783197.1. NC_001481.2.

Genome annotation databases

GeneIDi1502251.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L40021 Genomic RNA. Translation: AAA79324.1 .
RefSeqi NP_783197.1. NC_001481.2.

3D structure databases

ProteinModelPortali Q66915.
SMRi Q66915. Positions 129-662.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 1502251.

Family and domain databases

Gene3Di 2.60.120.20. 1 hit.
InterProi IPR004005. Calicivirus_coat.
IPR029053. Viral_coat.
[Graphical view ]
Pfami PF00915. Calici_coat. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "RNA transcripts derived from a cloned full-length copy of the feline calicivirus genome do not require VpG for infectivity."
    Sosnovtsev S.V., Green K.Y.
    Virology 210:383-390(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Cleavage of the feline calicivirus capsid precursor is mediated by a virus-encoded proteinase."
    Sosnovtsev S.V., Sosnovtseva S.A., Green K.Y.
    J. Virol. 72:3051-3059(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: CLEAVAGE, MUTAGENESIS OF GLU-124 AND ALA-125.
  3. "Feline calicivirus capsid protein expression and self-assembly in cultured feline cells."
    Geissler K., Parrish C.R., Schneider K., Truyen U.
    Vet. Microbiol. 69:63-66(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  4. "Analysis of protein-protein interactions in the feline calicivirus replication complex."
    Kaiser W.J., Chaudhry Y., Sosnovtsev S.V., Goodfellow I.G.
    J. Gen. Virol. 87:363-368(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH VP3 AND VPG.

Entry informationi

Entry nameiCAPSD_FCVUR
AccessioniPrimary (citable) accession number: Q66915
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 9, 2004
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3