Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q66915 (CAPSD_FCVUR) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Capsid protein
Alternative name(s):
Coat protein
Short name=CP

Cleaved into the following chain:

  1. Protein 40k
Gene names
ORF Names:ORF2
OrganismFeline calicivirus (strain Cat/United States/Urbana/1960) (FCV) [Reference proteome]
Taxonomic identifier292349 [NCBI]
Taxonomic lineageVirusesssRNA positive-strand viruses, no DNA stageCaliciviridaeVesivirus
Virus hostFelis catus (Cat) (Felis silvestris catus) [TaxID: 9685]

Protein attributes

Sequence length668 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Capsid protein self assembles to form an icosahedral capsid with a T=3 symmetry, about 38 nm in diameter, and consisting of 180 capsid proteins. A smaller form of capsid with a diameter of 23 nm might be capsid proteins assembled as icosahedron with T=1 symmetry. The capsid encapsulate the genomic RNA and VP2 proteins. Attaches virion to target cells by binding to feline junctional adhesion molecule A (F11R) and/or to alpha-2,6-linked sialic acid. Once attached, the virion is endocytosed. Acidification of the endosome induces conformational change of capsid protein thereby injecting virus genomic RNA into host cytoplasm By similarity.

Subunit structure

Homodimerizes, then multimerizes. May bind to VP3 and Vpg proteins. Binds to alpha-2,6-linked sialic acid at surface of target cells. Interacts with host F11R By similarity. Ref.4

Subcellular location

Virion. Host cytoplasm.

Post-translational modification

Cleaved by virus calcivirin to produce mature capsid protein.

Cleaved by host caspase-2 and caspase-6 to generate protein p40 By similarity.

Sequence similarities

Belongs to the caliciviridae capsid protein family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Propeptide1 – 124124 By similarity
PRO_0000036880
Chain125 – 668544Capsid protein By similarity
PRO_0000036881
Chain?465 – 668204Protein 40k
PRO_0000341625

Sites

Site124 – 1252Cleavage; by calicivirin

Experimental info

Mutagenesis1241E → H, K or L: Complete loss of precursor cleavage by viral calcivirin. Ref.2
Mutagenesis1241E → Q or D: Partial loss of precursor cleavage by calcivirin. Ref.2
Mutagenesis1251A → G, H, L, R or V: No effect on precursor cleavage by calcivirin. Ref.2
Mutagenesis1251A → P: Complete loss of precursor cleavage by viral calcivirin. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q66915 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: C1E38D92BB6E5FA6

FASTA66873,518
        10         20         30         40         50         60 
MCSTCANVLK YYNWDPHFKL VINPNKFLSI GFCDNPLMCC YPELLPEFGT VWDCDQSPLQ 

        70         80         90        100        110        120 
IYLESILGDD EWSSTYEAID PVVPPMHWNE AGKIFQPHPG VLMHHIIGEV AKAWDPNLPL 

       130        140        150        160        170        180 
FRLEADDGSI TAPEQGTVVG GVIAEPSSQM STAADMASGK SVDSEWEAFF SFHTSVNWST 

       190        200        210        220        230        240 
SETQGKILFK QSLGPLLNPY LEHLSKLYVA WSGSVEVRFS ISGSGVFGGK LAAIVVPPGV 

       250        260        270        280        290        300 
DPIQSTSMLQ YPHVLFDARQ VEPVIFTIPD LRSTLYHLMS DTDTTSLVIM VYNDLINPYA 

       310        320        330        340        350        360 
NDSNSSGCIV TVETKPGSDF KFHLLKPPGS MLTHGSVPSD LIPKTSSLWI GNRFWSDITD 

       370        380        390        400        410        420 
FVIRPFVFQA NRHFDFNQET AGWSTPRFRP ITVTISEKNG AKLGVGVATD FIVPGIPDGW 

       430        440        450        460        470        480 
PDTTIGEKLV PAGDYAITNG SGNDITTANQ YDAADIIRNN TNFKGMYICG SLQRAWGDKK 

       490        500        510        520        530        540 
ISNTAFITTA TVEGNDLIPS NVIDQTKIAI FQDNHVQDEV QTSDDTLALL GYTGIGEEAI 

       550        560        570        580        590        600 
GANRERVVRI STLPETGARG GNHPIFYKNS IKLGYVIRSI DVFNSQILHT SRQLSLNHYL 

       610        620        630        640        650        660 
LPPDSFAVYR IIDSNGSWFD VGIDFDGFSF VGVSDVGKLE FPLTASYMGI QLAKIRLASN 


IRSTMTKL 

« Hide

References

[1]"RNA transcripts derived from a cloned full-length copy of the feline calicivirus genome do not require VpG for infectivity."
Sosnovtsev S.V., Green K.Y.
Virology 210:383-390(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]"Cleavage of the feline calicivirus capsid precursor is mediated by a virus-encoded proteinase."
Sosnovtsev S.V., Sosnovtseva S.A., Green K.Y.
J. Virol. 72:3051-3059(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: CLEAVAGE, MUTAGENESIS OF GLU-124 AND ALA-125.
[3]"Feline calicivirus capsid protein expression and self-assembly in cultured feline cells."
Geissler K., Parrish C.R., Schneider K., Truyen U.
Vet. Microbiol. 69:63-66(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[4]"Analysis of protein-protein interactions in the feline calicivirus replication complex."
Kaiser W.J., Chaudhry Y., Sosnovtsev S.V., Goodfellow I.G.
J. Gen. Virol. 87:363-368(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH VP3 AND VPG.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L40021 Genomic RNA. Translation: AAA79324.1.
RefSeqNP_783197.1. NC_001481.2.

3D structure databases

ProteinModelPortalQ66915.
SMRQ66915. Positions 129-662.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1502251.

Family and domain databases

InterProIPR004005. Calicivirus_coat.
[Graphical view]
PfamPF00915. Calici_coat. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCAPSD_FCVUR
AccessionPrimary (citable) accession number: Q66915
Entry history
Integrated into UniProtKB/Swiss-Prot: November 9, 2004
Last sequence update: November 1, 1996
Last modified: May 29, 2013
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families