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Q66915

- CAPSD_FCVUR

UniProt

Q66915 - CAPSD_FCVUR

Protein

Capsid protein

Gene

ORF2

Organism
Feline calicivirus (strain Cat/United States/Urbana/1960) (FCV)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 57 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Capsid protein self assembles to form an icosahedral capsid with a T=3 symmetry, about 38 nm in diameter, and consisting of 180 capsid proteins. A smaller form of capsid with a diameter of 23 nm might be capsid proteins assembled as icosahedron with T=1 symmetry. The capsid encapsulate the genomic RNA and VP2 proteins. Attaches virion to target cells by binding to feline junctional adhesion molecule A (F11R) and/or to alpha-2,6-linked sialic acid. Once attached, the virion is endocytosed. Acidification of the endosome induces conformational change of capsid protein thereby injecting virus genomic RNA into host cytoplasm By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei124 – 1252Cleavage; by calicivirin

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Capsid protein
    Alternative name(s):
    Coat protein
    Short name:
    CP
    Cleaved into the following chain:
    Gene namesi
    ORF Names:ORF2
    OrganismiFeline calicivirus (strain Cat/United States/Urbana/1960) (FCV)
    Taxonomic identifieri292349 [NCBI]
    Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageCaliciviridaeVesivirus
    Virus hostiFelis catus (Cat) (Felis silvestris catus) [TaxID: 9685]
    ProteomesiUP000001098: Genome

    Subcellular locationi

    GO - Cellular componenti

    1. host cell cytoplasm Source: UniProtKB-SubCell
    2. T=3 icosahedral viral capsid Source: UniProtKB-KW

    Keywords - Cellular componenti

    Capsid protein, Host cytoplasm, T=3 icosahedral capsid protein, Virion

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi124 – 1241E → H, K or L: Complete loss of precursor cleavage by viral calcivirin. 1 Publication
    Mutagenesisi124 – 1241E → Q or D: Partial loss of precursor cleavage by calcivirin. 1 Publication
    Mutagenesisi125 – 1251A → G, H, L, R or V: No effect on precursor cleavage by calcivirin. 1 Publication
    Mutagenesisi125 – 1251A → P: Complete loss of precursor cleavage by viral calcivirin. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Propeptidei1 – 124124By similarityPRO_0000036880Add
    BLAST
    Chaini125 – 668544Capsid proteinBy similarityPRO_0000036881Add
    BLAST
    Chaini?465 – 668204Protein 40kPRO_0000341625Add
    BLAST

    Post-translational modificationi

    Cleaved by virus calcivirin to produce mature capsid protein.
    Cleaved by host caspase-2 and caspase-6 to generate protein p40.By similarity

    Interactioni

    Subunit structurei

    Homodimerizes, then multimerizes. May bind to VP3 and Vpg proteins. Binds to alpha-2,6-linked sialic acid at surface of target cells. Interacts with host F11R By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ66915.
    SMRiQ66915. Positions 129-662.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Family and domain databases

    Gene3Di2.60.120.20. 1 hit.
    InterProiIPR004005. Calicivirus_coat.
    IPR029053. Viral_coat.
    [Graphical view]
    PfamiPF00915. Calici_coat. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q66915-1 [UniParc]FASTAAdd to Basket

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    MCSTCANVLK YYNWDPHFKL VINPNKFLSI GFCDNPLMCC YPELLPEFGT    50
    VWDCDQSPLQ IYLESILGDD EWSSTYEAID PVVPPMHWNE AGKIFQPHPG 100
    VLMHHIIGEV AKAWDPNLPL FRLEADDGSI TAPEQGTVVG GVIAEPSSQM 150
    STAADMASGK SVDSEWEAFF SFHTSVNWST SETQGKILFK QSLGPLLNPY 200
    LEHLSKLYVA WSGSVEVRFS ISGSGVFGGK LAAIVVPPGV DPIQSTSMLQ 250
    YPHVLFDARQ VEPVIFTIPD LRSTLYHLMS DTDTTSLVIM VYNDLINPYA 300
    NDSNSSGCIV TVETKPGSDF KFHLLKPPGS MLTHGSVPSD LIPKTSSLWI 350
    GNRFWSDITD FVIRPFVFQA NRHFDFNQET AGWSTPRFRP ITVTISEKNG 400
    AKLGVGVATD FIVPGIPDGW PDTTIGEKLV PAGDYAITNG SGNDITTANQ 450
    YDAADIIRNN TNFKGMYICG SLQRAWGDKK ISNTAFITTA TVEGNDLIPS 500
    NVIDQTKIAI FQDNHVQDEV QTSDDTLALL GYTGIGEEAI GANRERVVRI 550
    STLPETGARG GNHPIFYKNS IKLGYVIRSI DVFNSQILHT SRQLSLNHYL 600
    LPPDSFAVYR IIDSNGSWFD VGIDFDGFSF VGVSDVGKLE FPLTASYMGI 650
    QLAKIRLASN IRSTMTKL 668
    Length:668
    Mass (Da):73,518
    Last modified:November 1, 1996 - v1
    Checksum:iC1E38D92BB6E5FA6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L40021 Genomic RNA. Translation: AAA79324.1.
    RefSeqiNP_783197.1. NC_001481.2.

    Genome annotation databases

    GeneIDi1502251.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L40021 Genomic RNA. Translation: AAA79324.1 .
    RefSeqi NP_783197.1. NC_001481.2.

    3D structure databases

    ProteinModelPortali Q66915.
    SMRi Q66915. Positions 129-662.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 1502251.

    Family and domain databases

    Gene3Di 2.60.120.20. 1 hit.
    InterProi IPR004005. Calicivirus_coat.
    IPR029053. Viral_coat.
    [Graphical view ]
    Pfami PF00915. Calici_coat. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "RNA transcripts derived from a cloned full-length copy of the feline calicivirus genome do not require VpG for infectivity."
      Sosnovtsev S.V., Green K.Y.
      Virology 210:383-390(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    2. "Cleavage of the feline calicivirus capsid precursor is mediated by a virus-encoded proteinase."
      Sosnovtsev S.V., Sosnovtseva S.A., Green K.Y.
      J. Virol. 72:3051-3059(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: CLEAVAGE, MUTAGENESIS OF GLU-124 AND ALA-125.
    3. "Feline calicivirus capsid protein expression and self-assembly in cultured feline cells."
      Geissler K., Parrish C.R., Schneider K., Truyen U.
      Vet. Microbiol. 69:63-66(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    4. "Analysis of protein-protein interactions in the feline calicivirus replication complex."
      Kaiser W.J., Chaudhry Y., Sosnovtsev S.V., Goodfellow I.G.
      J. Gen. Virol. 87:363-368(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH VP3 AND VPG.

    Entry informationi

    Entry nameiCAPSD_FCVUR
    AccessioniPrimary (citable) accession number: Q66915
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 9, 2004
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 57 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3