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Q66802

- L_EBOSM

UniProt

Q66802 - L_EBOSM

Protein

RNA-directed RNA polymerase L

Gene

L

Organism
Sudan ebolavirus (strain Maleo-79) (SEBOV) (Sudan Ebola virus)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 79 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The transcriptase stutters on a specific sequence, resulting on a cotranscriptional editing of the glycoprotein (GP) mRNA. The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals By similarity.By similarity

    Catalytic activityi

    Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation
    S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. mRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-EC
    3. RNA-directed RNA polymerase activity Source: UniProtKB-KW

    Keywords - Molecular functioni

    Methyltransferase, Nucleotidyltransferase, RNA-directed RNA polymerase, Transferase

    Keywords - Biological processi

    mRNA capping, mRNA processing, Viral RNA replication

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding, S-adenosyl-L-methionine

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    RNA-directed RNA polymerase L
    Short name:
    Protein L
    Alternative name(s):
    Large structural protein
    Replicase
    Transcriptase
    Including the following 3 domains:
    mRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.56)
    mRNA guanylyltransferase (EC:2.7.7.-)
    Gene namesi
    Name:L
    OrganismiSudan ebolavirus (strain Maleo-79) (SEBOV) (Sudan Ebola virus)
    Taxonomic identifieri128949 [NCBI]
    Taxonomic lineageiVirusesssRNA negative-strand virusesMononegaviralesFiloviridaeEbolavirus
    Virus hostiEpomops franqueti (Franquet's epauleted fruit bat) [TaxID: 77231]
    Homo sapiens (Human) [TaxID: 9606]
    Myonycteris torquata (Little collared fruit bat) [TaxID: 77243]

    Subcellular locationi

    Host cytoplasm. Virion By similarity

    GO - Cellular componenti

    1. host cell cytoplasm Source: UniProtKB-SubCell
    2. virion Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host cytoplasm, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 22102210RNA-directed RNA polymerase LPRO_0000222168Add
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini626 – 810185RdRp catalyticPROSITE-ProRule annotationAdd
    BLAST
    Domaini1802 – 2000199Mononegavirus-type SAM-dependent 2'-O-MTasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 mononegavirus-type SAM-dependent 2'-O-MTase domain.PROSITE-ProRule annotation
    Contains 1 RdRp catalytic domain.PROSITE-ProRule annotation

    Family and domain databases

    InterProiIPR026890. Mononeg_mRNAcap.
    IPR014023. Mononeg_RNA_pol_cat.
    IPR017235. RNA-dir_pol_L_filovirus.
    IPR025786. RNA-direct_RNA_pol_L.
    [Graphical view]
    PfamiPF14318. Mononeg_mRNAcap. 1 hit.
    PF00946. Mononeg_RNA_pol. 1 hit.
    [Graphical view]
    PIRSFiPIRSF037548. RNA_pol_Filoviridae. 1 hit.
    TIGRFAMsiTIGR04198. paramyx_RNAcap. 1 hit.
    PROSITEiPS50526. RDRP_SSRNA_NEG_NONSEG. 1 hit.
    PS51590. SAM_MT_MNV_L. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q66802-1 [UniParc]FASTAAdd to Basket

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    MMATQHTQYP DARLSSPIVL DQCDLVTRAC GLYSEYSLNP KLRTCRLPKH     50
    IYRLKYDAIV LRFISDVPVA TIPIDYIAPM LINVLADSKN APLEPPCLSF 100
    LDEIVNYTVQ DAAFLNYYMN QIKTQEGVIT DQLKQNIRRV IHKNRYLSAL 150
    FFWHDLSILT RRGRMNRGNV RSTWFVTNEV VDILGYGDYI FWKIPIALLP 200
    MNSANVPHAS TDWYQPNIFK EAIQGHTHII SVSTAEVLIM CKDLVTSRFN 250
    TLLIAELARL EDPVSADYPL VDDIQSLYNA GDYLLSILGS EGYQIIKYLE 300
    PLCLAKIQLC SQYTERKGRF LTQMHLAVIQ TLRELLLNRG LKKSQLSKIR 350
    EFHQLLLRLR STPQQLCELF SIQKHWGHPV LHSEKAIQKV KNHATVLKAL 400
    RPIIIFETYC VFKYSVAKHF FDSQGTWYSV ISDRCLTPGL NSYIRRNQFP 450
    PLPMIKDLLW EFYHLDHPPL FSTKIISDLS IFIKDRATAV EQTCWDAVFE 500
    PNVLGYSPPY RFNTKRVPEQ FLEQEDFSIE SVLQYAQELR YLLPQNRNFS 550
    FSLKEKELNV GRTFGKLPYL TRNVQTLCEA LLADGLAKAF PSNMMVVTER 600
    EQKESLLHQA SWHHTSDDFG EHATVRGSSF VTDLEKYNLA FRYEFTAPFI 650
    KYCNQCYGVR NVFDWMHFLI PQCYMHVSDY YNPPHNVTLE NREYPPEGPS 700
    AYRGHLGGIE GLQQKLWTSI SCAQISLVEI KTGFKLRSAV MGDNQCITVL 750
    SVFPLESSPN EQERCAEDNA ARVAASLAKV TSACGIFLKP DETFVHSGFI 800
    YFGPKQYLNG IQLPQSLKTA ARMAPLSDAI FDDLQGTLAS IGTAFERSIS 850
    ETRHILPSRV AAAFHTYFSV RILQHHHLGF HKGSDLGQLA INKPLDFGTI 900
    ALSLAVPQVL GGLSFLNPEK CLYRNLGDPV TSGLFQLKHY LSMVGMSDIF 950
    HALVAKSPGN CSAIDFVLNP GGLNVPGSQD LTSFLRQIVR RSITLSARNK 1000
    LINTLFHASA DLEDELVCKW LLSSTPVMSR FAADIFSRTP SGKRLQILGY 1050
    LEGTRTLLAS KMISNNAETP ILERLRKITL QRWNLWFSYL DHCDSALMEA 1100
    IQPIRCTVDI AQILREYSWA HILGGRQLIG ATLPCIPEQF QTTWLKPYEQ 1150
    CVECSSTNNS SPYVSVALKR NVVSAWPDAS RLGWTIGDGI PYIGSRTEDK 1200
    IGQPAIKPRC PSAALREAIE LTSRLTWVTQ GSANSDQLIR PFLEARVNLS 1250
    VQEILQMTPS HYSGNIVHRY NDQYSPHSFM ANRMSNTATR LMVSTNTLGE 1300
    FSGGGQAARD SNIIFQNVIN FAVALYDIRF RNTCTSSIQY HRAHIHLTDC 1350
    CTREVPAQYL TYTTTLNLDL SKYRNNELIY DSEPLRGGLN CNLSIDSPLM 1400
    KGPRLNIIED DLIRLPHLSG WELAKTVLQS IISDSSNSST DPISSGETRS 1450
    FTTHFLTYPK IGLLYSFGAL ISFYLGNTIL CTKKIGLTEF LYYLQNQIHN 1500
    LSHRSLRIFK PTFRHSSVMS RLMDIDPNFS IYIGGTAGDR GLSDAARLFL 1550
    RIAISTFLSF VEEWVIFRKA NIPLWVVYPL EGQRPDPPGE FLNRVKSLIV 1600
    GIEDDKNKGS ILSRSEEKCS SNLVYNCKST ASNFFHASLA YWRGRHRPKK 1650
    TIGATKATTA PHIILPLGNS DRPPGLDLNQ SNDTFIPTRI KQIVQGDSRN 1700
    DRTTTTRLPP QSRSTPTSAT EPPTKIYEGS TTYRGKSTDT HLDEGHNAKE 1750
    FPFNPHRLVV PFFKLTKDGE YSIEPSPEES RSNIKGLLQH LRTMVDTTIY 1800
    CRFTGIVSSM HYKLDEVLWE YNKFESAVTL AEGEGSGALL LIQKYGVKKL 1850
    FLNTLATEHS IESEVISGYT TPRMLLSVMP RTHRGELEVI LNNSASQITD 1900
    ITHRDWFSNQ KNRIPNDVDI ITMDAETTEN LDRSRLYEAV YTIICNHINP 1950
    KTLKVVILKV FLSDLDGMCW INNYLAPMFG SGYLIKPITS SARSSEWYLC 2000
    LSNLLSTLRT TQHQTQANCL HVVQCALQQQ VQRGSYWLSH LTKYTTSRLH 2050
    NSYIAFGFPS LEKVLYHRYN LVDSRNGPLV SITRHLALLQ TEIRELVTDY 2100
    NQLRQSRTQT YHFIKTSKGR ITKLVNDYLR FELVIRALKN NSTWHHELYL 2150
    LPELIGVCHR FNHTRNCTCS ERFLVQTLYL HRMSDAEIKL MDRLTSLVNM 2200
    FPEGFRSSSV 2210
    Length:2,210
    Mass (Da):251,258
    Last modified:November 1, 1996 - v1
    Checksum:iF50E6B624951AEA4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U23458 Genomic RNA. Translation: AAA79970.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U23458 Genomic RNA. Translation: AAA79970.1 .

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR026890. Mononeg_mRNAcap.
    IPR014023. Mononeg_RNA_pol_cat.
    IPR017235. RNA-dir_pol_L_filovirus.
    IPR025786. RNA-direct_RNA_pol_L.
    [Graphical view ]
    Pfami PF14318. Mononeg_mRNAcap. 1 hit.
    PF00946. Mononeg_RNA_pol. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF037548. RNA_pol_Filoviridae. 1 hit.
    TIGRFAMsi TIGR04198. paramyx_RNAcap. 1 hit.
    PROSITEi PS50526. RDRP_SSRNA_NEG_NONSEG. 1 hit.
    PS51590. SAM_MT_MNV_L. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Sanchez A., Trappier S., Nichol S.T.
      Submitted (MAR-1995) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

    Entry informationi

    Entry nameiL_EBOSM
    AccessioniPrimary (citable) accession number: Q66802
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 79 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Multifunctional enzyme

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3