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Q66802 (L_EBOSM) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
RNA-directed RNA polymerase L

Short name=Protein L
Alternative name(s):
Large structural protein
Replicase
Transcriptase

Including the following 3 domains:

  1. RNA-directed RNA polymerase
    EC=2.7.7.48
  2. mRNA (guanine-N(7)-)-methyltransferase
    EC=2.1.1.56
  3. mRNA guanylyltransferase
    EC=2.7.7.-
Gene names
Name:L
OrganismSudan ebolavirus (strain Maleo-79) (SEBOV) (Sudan Ebola virus)
Taxonomic identifier128949 [NCBI]
Taxonomic lineageVirusesssRNA negative-strand virusesMononegaviralesFiloviridaeEbolavirus
Virus hostEpomops franqueti (Franquet's epauleted fruit bat) [TaxID: 77231]
Homo sapiens (Human) [TaxID: 9606]
Myonycteris torquata (Little collared fruit bat) [TaxID: 77243]

Protein attributes

Sequence length2210 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The transcriptase stutters on a specific sequence, resulting on a cotranscriptional editing of the glycoprotein (GP) mRNA. The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals By similarity.

Catalytic activity

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.

Subcellular location

Host cytoplasm. Virion By similarity.

Sequence similarities

Contains 1 mononegavirus-type SAM-dependent 2'-O-MTase domain.

Contains 1 RdRp catalytic domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 22102210RNA-directed RNA polymerase L
PRO_0000222168

Regions

Domain626 – 810185RdRp catalytic
Domain1802 – 2000199Mononegavirus-type SAM-dependent 2'-O-MTase

Sequences

Sequence LengthMass (Da)Tools
Q66802 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: F50E6B624951AEA4

FASTA2,210251,258
        10         20         30         40         50         60 
MMATQHTQYP DARLSSPIVL DQCDLVTRAC GLYSEYSLNP KLRTCRLPKH IYRLKYDAIV 

        70         80         90        100        110        120 
LRFISDVPVA TIPIDYIAPM LINVLADSKN APLEPPCLSF LDEIVNYTVQ DAAFLNYYMN 

       130        140        150        160        170        180 
QIKTQEGVIT DQLKQNIRRV IHKNRYLSAL FFWHDLSILT RRGRMNRGNV RSTWFVTNEV 

       190        200        210        220        230        240 
VDILGYGDYI FWKIPIALLP MNSANVPHAS TDWYQPNIFK EAIQGHTHII SVSTAEVLIM 

       250        260        270        280        290        300 
CKDLVTSRFN TLLIAELARL EDPVSADYPL VDDIQSLYNA GDYLLSILGS EGYQIIKYLE 

       310        320        330        340        350        360 
PLCLAKIQLC SQYTERKGRF LTQMHLAVIQ TLRELLLNRG LKKSQLSKIR EFHQLLLRLR 

       370        380        390        400        410        420 
STPQQLCELF SIQKHWGHPV LHSEKAIQKV KNHATVLKAL RPIIIFETYC VFKYSVAKHF 

       430        440        450        460        470        480 
FDSQGTWYSV ISDRCLTPGL NSYIRRNQFP PLPMIKDLLW EFYHLDHPPL FSTKIISDLS 

       490        500        510        520        530        540 
IFIKDRATAV EQTCWDAVFE PNVLGYSPPY RFNTKRVPEQ FLEQEDFSIE SVLQYAQELR 

       550        560        570        580        590        600 
YLLPQNRNFS FSLKEKELNV GRTFGKLPYL TRNVQTLCEA LLADGLAKAF PSNMMVVTER 

       610        620        630        640        650        660 
EQKESLLHQA SWHHTSDDFG EHATVRGSSF VTDLEKYNLA FRYEFTAPFI KYCNQCYGVR 

       670        680        690        700        710        720 
NVFDWMHFLI PQCYMHVSDY YNPPHNVTLE NREYPPEGPS AYRGHLGGIE GLQQKLWTSI 

       730        740        750        760        770        780 
SCAQISLVEI KTGFKLRSAV MGDNQCITVL SVFPLESSPN EQERCAEDNA ARVAASLAKV 

       790        800        810        820        830        840 
TSACGIFLKP DETFVHSGFI YFGPKQYLNG IQLPQSLKTA ARMAPLSDAI FDDLQGTLAS 

       850        860        870        880        890        900 
IGTAFERSIS ETRHILPSRV AAAFHTYFSV RILQHHHLGF HKGSDLGQLA INKPLDFGTI 

       910        920        930        940        950        960 
ALSLAVPQVL GGLSFLNPEK CLYRNLGDPV TSGLFQLKHY LSMVGMSDIF HALVAKSPGN 

       970        980        990       1000       1010       1020 
CSAIDFVLNP GGLNVPGSQD LTSFLRQIVR RSITLSARNK LINTLFHASA DLEDELVCKW 

      1030       1040       1050       1060       1070       1080 
LLSSTPVMSR FAADIFSRTP SGKRLQILGY LEGTRTLLAS KMISNNAETP ILERLRKITL 

      1090       1100       1110       1120       1130       1140 
QRWNLWFSYL DHCDSALMEA IQPIRCTVDI AQILREYSWA HILGGRQLIG ATLPCIPEQF 

      1150       1160       1170       1180       1190       1200 
QTTWLKPYEQ CVECSSTNNS SPYVSVALKR NVVSAWPDAS RLGWTIGDGI PYIGSRTEDK 

      1210       1220       1230       1240       1250       1260 
IGQPAIKPRC PSAALREAIE LTSRLTWVTQ GSANSDQLIR PFLEARVNLS VQEILQMTPS 

      1270       1280       1290       1300       1310       1320 
HYSGNIVHRY NDQYSPHSFM ANRMSNTATR LMVSTNTLGE FSGGGQAARD SNIIFQNVIN 

      1330       1340       1350       1360       1370       1380 
FAVALYDIRF RNTCTSSIQY HRAHIHLTDC CTREVPAQYL TYTTTLNLDL SKYRNNELIY 

      1390       1400       1410       1420       1430       1440 
DSEPLRGGLN CNLSIDSPLM KGPRLNIIED DLIRLPHLSG WELAKTVLQS IISDSSNSST 

      1450       1460       1470       1480       1490       1500 
DPISSGETRS FTTHFLTYPK IGLLYSFGAL ISFYLGNTIL CTKKIGLTEF LYYLQNQIHN 

      1510       1520       1530       1540       1550       1560 
LSHRSLRIFK PTFRHSSVMS RLMDIDPNFS IYIGGTAGDR GLSDAARLFL RIAISTFLSF 

      1570       1580       1590       1600       1610       1620 
VEEWVIFRKA NIPLWVVYPL EGQRPDPPGE FLNRVKSLIV GIEDDKNKGS ILSRSEEKCS 

      1630       1640       1650       1660       1670       1680 
SNLVYNCKST ASNFFHASLA YWRGRHRPKK TIGATKATTA PHIILPLGNS DRPPGLDLNQ 

      1690       1700       1710       1720       1730       1740 
SNDTFIPTRI KQIVQGDSRN DRTTTTRLPP QSRSTPTSAT EPPTKIYEGS TTYRGKSTDT 

      1750       1760       1770       1780       1790       1800 
HLDEGHNAKE FPFNPHRLVV PFFKLTKDGE YSIEPSPEES RSNIKGLLQH LRTMVDTTIY 

      1810       1820       1830       1840       1850       1860 
CRFTGIVSSM HYKLDEVLWE YNKFESAVTL AEGEGSGALL LIQKYGVKKL FLNTLATEHS 

      1870       1880       1890       1900       1910       1920 
IESEVISGYT TPRMLLSVMP RTHRGELEVI LNNSASQITD ITHRDWFSNQ KNRIPNDVDI 

      1930       1940       1950       1960       1970       1980 
ITMDAETTEN LDRSRLYEAV YTIICNHINP KTLKVVILKV FLSDLDGMCW INNYLAPMFG 

      1990       2000       2010       2020       2030       2040 
SGYLIKPITS SARSSEWYLC LSNLLSTLRT TQHQTQANCL HVVQCALQQQ VQRGSYWLSH 

      2050       2060       2070       2080       2090       2100 
LTKYTTSRLH NSYIAFGFPS LEKVLYHRYN LVDSRNGPLV SITRHLALLQ TEIRELVTDY 

      2110       2120       2130       2140       2150       2160 
NQLRQSRTQT YHFIKTSKGR ITKLVNDYLR FELVIRALKN NSTWHHELYL LPELIGVCHR 

      2170       2180       2190       2200       2210 
FNHTRNCTCS ERFLVQTLYL HRMSDAEIKL MDRLTSLVNM FPEGFRSSSV 

« Hide

References

[1]Sanchez A., Trappier S., Nichol S.T.
Submitted (MAR-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U23458 Genomic RNA. Translation: AAA79970.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR026890. Mononeg_mRNAcap.
IPR014023. Mononeg_RNA_pol_cat.
IPR017235. RNA-dir_pol_L_filovirus.
IPR025786. RNA-direct_RNA_pol_L.
[Graphical view]
PfamPF14318. Mononeg_mRNAcap. 1 hit.
PF00946. Mononeg_RNA_pol. 1 hit.
[Graphical view]
PIRSFPIRSF037548. RNA_pol_Filoviridae. 1 hit.
TIGRFAMsTIGR04198. paramyx_RNAcap. 1 hit.
PROSITEPS50526. RDRP_SSRNA_NEG_NONSEG. 1 hit.
PS51590. SAM_MT_MNV_L. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameL_EBOSM
AccessionPrimary (citable) accession number: Q66802
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families