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Q66802

- L_EBOSM

UniProt

Q66802 - L_EBOSM

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Protein

RNA-directed RNA polymerase L

Gene
L
Organism
Sudan ebolavirus (strain Maleo-79) (SEBOV) (Sudan Ebola virus)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The transcriptase stutters on a specific sequence, resulting on a cotranscriptional editing of the glycoprotein (GP) mRNA. The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals By similarity.

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. mRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-EC
  3. RNA-directed RNA polymerase activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Nucleotidyltransferase, RNA-directed RNA polymerase, Transferase

Keywords - Biological processi

mRNA capping, mRNA processing, Viral RNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
RNA-directed RNA polymerase L
Short name:
Protein L
Alternative name(s):
Large structural protein
Replicase
Transcriptase
Including the following 3 domains:
mRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.56)
mRNA guanylyltransferase (EC:2.7.7.-)
Gene namesi
Name:L
OrganismiSudan ebolavirus (strain Maleo-79) (SEBOV) (Sudan Ebola virus)
Taxonomic identifieri128949 [NCBI]
Taxonomic lineageiVirusesssRNA negative-strand virusesMononegaviralesFiloviridaeEbolavirus
Virus hostiEpomops franqueti (Franquet's epauleted fruit bat) [TaxID: 77231]
Homo sapiens (Human) [TaxID: 9606]
Myonycteris torquata (Little collared fruit bat) [TaxID: 77243]

Subcellular locationi

Host cytoplasm. Virion By similarity

GO - Cellular componenti

  1. host cell cytoplasm Source: UniProtKB-SubCell
  2. virion Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 22102210RNA-directed RNA polymerase LPRO_0000222168Add
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini626 – 810185RdRp catalyticAdd
BLAST
Domaini1802 – 2000199Mononegavirus-type SAM-dependent 2'-O-MTaseAdd
BLAST

Sequence similaritiesi

Family and domain databases

InterProiIPR026890. Mononeg_mRNAcap.
IPR014023. Mononeg_RNA_pol_cat.
IPR017235. RNA-dir_pol_L_filovirus.
IPR025786. RNA-direct_RNA_pol_L.
[Graphical view]
PfamiPF14318. Mononeg_mRNAcap. 1 hit.
PF00946. Mononeg_RNA_pol. 1 hit.
[Graphical view]
PIRSFiPIRSF037548. RNA_pol_Filoviridae. 1 hit.
TIGRFAMsiTIGR04198. paramyx_RNAcap. 1 hit.
PROSITEiPS50526. RDRP_SSRNA_NEG_NONSEG. 1 hit.
PS51590. SAM_MT_MNV_L. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q66802-1 [UniParc]FASTAAdd to Basket

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MMATQHTQYP DARLSSPIVL DQCDLVTRAC GLYSEYSLNP KLRTCRLPKH     50
IYRLKYDAIV LRFISDVPVA TIPIDYIAPM LINVLADSKN APLEPPCLSF 100
LDEIVNYTVQ DAAFLNYYMN QIKTQEGVIT DQLKQNIRRV IHKNRYLSAL 150
FFWHDLSILT RRGRMNRGNV RSTWFVTNEV VDILGYGDYI FWKIPIALLP 200
MNSANVPHAS TDWYQPNIFK EAIQGHTHII SVSTAEVLIM CKDLVTSRFN 250
TLLIAELARL EDPVSADYPL VDDIQSLYNA GDYLLSILGS EGYQIIKYLE 300
PLCLAKIQLC SQYTERKGRF LTQMHLAVIQ TLRELLLNRG LKKSQLSKIR 350
EFHQLLLRLR STPQQLCELF SIQKHWGHPV LHSEKAIQKV KNHATVLKAL 400
RPIIIFETYC VFKYSVAKHF FDSQGTWYSV ISDRCLTPGL NSYIRRNQFP 450
PLPMIKDLLW EFYHLDHPPL FSTKIISDLS IFIKDRATAV EQTCWDAVFE 500
PNVLGYSPPY RFNTKRVPEQ FLEQEDFSIE SVLQYAQELR YLLPQNRNFS 550
FSLKEKELNV GRTFGKLPYL TRNVQTLCEA LLADGLAKAF PSNMMVVTER 600
EQKESLLHQA SWHHTSDDFG EHATVRGSSF VTDLEKYNLA FRYEFTAPFI 650
KYCNQCYGVR NVFDWMHFLI PQCYMHVSDY YNPPHNVTLE NREYPPEGPS 700
AYRGHLGGIE GLQQKLWTSI SCAQISLVEI KTGFKLRSAV MGDNQCITVL 750
SVFPLESSPN EQERCAEDNA ARVAASLAKV TSACGIFLKP DETFVHSGFI 800
YFGPKQYLNG IQLPQSLKTA ARMAPLSDAI FDDLQGTLAS IGTAFERSIS 850
ETRHILPSRV AAAFHTYFSV RILQHHHLGF HKGSDLGQLA INKPLDFGTI 900
ALSLAVPQVL GGLSFLNPEK CLYRNLGDPV TSGLFQLKHY LSMVGMSDIF 950
HALVAKSPGN CSAIDFVLNP GGLNVPGSQD LTSFLRQIVR RSITLSARNK 1000
LINTLFHASA DLEDELVCKW LLSSTPVMSR FAADIFSRTP SGKRLQILGY 1050
LEGTRTLLAS KMISNNAETP ILERLRKITL QRWNLWFSYL DHCDSALMEA 1100
IQPIRCTVDI AQILREYSWA HILGGRQLIG ATLPCIPEQF QTTWLKPYEQ 1150
CVECSSTNNS SPYVSVALKR NVVSAWPDAS RLGWTIGDGI PYIGSRTEDK 1200
IGQPAIKPRC PSAALREAIE LTSRLTWVTQ GSANSDQLIR PFLEARVNLS 1250
VQEILQMTPS HYSGNIVHRY NDQYSPHSFM ANRMSNTATR LMVSTNTLGE 1300
FSGGGQAARD SNIIFQNVIN FAVALYDIRF RNTCTSSIQY HRAHIHLTDC 1350
CTREVPAQYL TYTTTLNLDL SKYRNNELIY DSEPLRGGLN CNLSIDSPLM 1400
KGPRLNIIED DLIRLPHLSG WELAKTVLQS IISDSSNSST DPISSGETRS 1450
FTTHFLTYPK IGLLYSFGAL ISFYLGNTIL CTKKIGLTEF LYYLQNQIHN 1500
LSHRSLRIFK PTFRHSSVMS RLMDIDPNFS IYIGGTAGDR GLSDAARLFL 1550
RIAISTFLSF VEEWVIFRKA NIPLWVVYPL EGQRPDPPGE FLNRVKSLIV 1600
GIEDDKNKGS ILSRSEEKCS SNLVYNCKST ASNFFHASLA YWRGRHRPKK 1650
TIGATKATTA PHIILPLGNS DRPPGLDLNQ SNDTFIPTRI KQIVQGDSRN 1700
DRTTTTRLPP QSRSTPTSAT EPPTKIYEGS TTYRGKSTDT HLDEGHNAKE 1750
FPFNPHRLVV PFFKLTKDGE YSIEPSPEES RSNIKGLLQH LRTMVDTTIY 1800
CRFTGIVSSM HYKLDEVLWE YNKFESAVTL AEGEGSGALL LIQKYGVKKL 1850
FLNTLATEHS IESEVISGYT TPRMLLSVMP RTHRGELEVI LNNSASQITD 1900
ITHRDWFSNQ KNRIPNDVDI ITMDAETTEN LDRSRLYEAV YTIICNHINP 1950
KTLKVVILKV FLSDLDGMCW INNYLAPMFG SGYLIKPITS SARSSEWYLC 2000
LSNLLSTLRT TQHQTQANCL HVVQCALQQQ VQRGSYWLSH LTKYTTSRLH 2050
NSYIAFGFPS LEKVLYHRYN LVDSRNGPLV SITRHLALLQ TEIRELVTDY 2100
NQLRQSRTQT YHFIKTSKGR ITKLVNDYLR FELVIRALKN NSTWHHELYL 2150
LPELIGVCHR FNHTRNCTCS ERFLVQTLYL HRMSDAEIKL MDRLTSLVNM 2200
FPEGFRSSSV 2210
Length:2,210
Mass (Da):251,258
Last modified:November 1, 1996 - v1
Checksum:iF50E6B624951AEA4
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U23458 Genomic RNA. Translation: AAA79970.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U23458 Genomic RNA. Translation: AAA79970.1 .

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR026890. Mononeg_mRNAcap.
IPR014023. Mononeg_RNA_pol_cat.
IPR017235. RNA-dir_pol_L_filovirus.
IPR025786. RNA-direct_RNA_pol_L.
[Graphical view ]
Pfami PF14318. Mononeg_mRNAcap. 1 hit.
PF00946. Mononeg_RNA_pol. 1 hit.
[Graphical view ]
PIRSFi PIRSF037548. RNA_pol_Filoviridae. 1 hit.
TIGRFAMsi TIGR04198. paramyx_RNAcap. 1 hit.
PROSITEi PS50526. RDRP_SSRNA_NEG_NONSEG. 1 hit.
PS51590. SAM_MT_MNV_L. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Sanchez A., Trappier S., Nichol S.T.
    Submitted (MAR-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiL_EBOSM
AccessioniPrimary (citable) accession number: Q66802
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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