Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q66800

- VSGP_EBORR

UniProt

Q66800 - VSGP_EBORR

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Pre-small/secreted glycoprotein

Gene

GP

Organism
Reston ebolavirus (strain Reston-89) (REBOV) (Reston Ebola virus)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

sGP seems to possess an anti-inflammatory activity as it can reverse the barrier-decreasing effects of TNF alpha. Might therefore contribute to the lack of inflammatory reaction seen during infection in spite the of extensive necrosis and massive virus production. Does not seem to be involved in activation of primary macrophages. Does not seem to interact specifically with neutrophils (By similarity).By similarity
Delta-peptide does not seem to be involved in activation of primary macrophages.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei325 – 3262Cleavage; by host furinBy similarity

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-small/secreted glycoprotein
Short name:
pre-sGP
Cleaved into the following 2 chains:
Gene namesi
Name:GP
OrganismiReston ebolavirus (strain Reston-89) (REBOV) (Reston Ebola virus)
Taxonomic identifieri386032 [NCBI]
Taxonomic lineageiVirusesssRNA negative-strand virusesMononegaviralesFiloviridaeEbolavirus
Virus hostiEpomops franqueti (Franquet's epauleted fruit bat) [TaxID: 77231]
Homo sapiens (Human) [TaxID: 9606]
Myonycteris torquata (Little collared fruit bat) [TaxID: 77243]
Sus scrofa (Pig) [TaxID: 9823]
ProteomesiUP000007207: Genome

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3333Sequence AnalysisAdd
BLAST
Chaini34 – 367334Pre-small/secreted glycoproteinBy similarityPRO_0000037497Add
BLAST
Chaini34 – 325292Small/secreted glycoproteinBy similarityPRO_0000037498Add
BLAST
Chaini326 – 36742Delta-peptideBy similarityPRO_0000037499Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi41 – 411N-linked (GlcNAc...); by hostSequence Analysis
Disulfide bondi54 – 54InterchainBy similarity
Disulfide bondi109 ↔ 136By similarity
Disulfide bondi122 ↔ 148By similarity
Glycosylationi205 – 2051N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi229 – 2291N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi239 – 2391N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi258 – 2581N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi269 – 2691N-linked (GlcNAc...); by hostSequence Analysis
Disulfide bondi307 – 307InterchainBy similarity

Post-translational modificationi

Pre-sGP is N-glycosylated. This precursor is processed into mature sGP and delta-peptide by host furin or furin-like proteases. The cleavage site corresponds to the furin optimal cleavage sequence [KR]-X-[KR]-R. Both cleavage fragments contain sialic acid, but only the delta-peptide is O-glycosylated (By similarity).By similarity

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

sGP is a homodimer; disulfide-linked. The homodimers are linked by two disulfide bonds in a parallel orientation. Delta-peptide is a monomer (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ66800.
SMRiQ66800. Positions 33-285.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the filoviruses glycoprotein family.Curated

Keywords - Domaini

Signal

Family and domain databases

InterProiIPR014625. GPC_FiloV.
IPR002561. GPC_filovir-type_extra_dom.
[Graphical view]
PfamiPF01611. Filo_glycop. 1 hit.
[Graphical view]
PIRSFiPIRSF036874. GPC_FiloV. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q66800-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGSGYQLLQL PRERFRKTSF LVWVIILFQR AISMPLGIVT NSTLKATEID
60 70 80 90 100
QLVCRDKLSS TSQLKSVGLN LEGNGIATDV PSATKRWGFR SGVPPKVVSY
110 120 130 140 150
EAGEWAENCY NLEIKKSDGS ECLPLPPDGV RGFPRCRYVH KVQGTGPCPG
160 170 180 190 200
DLAFHKNGAF FLYDRLASTV IYRGTTFAEG VVAFLILSEP KKHFWKATPA
210 220 230 240 250
HEPVNTTDDS TSYYMTLTLS YEMSNFGGNE SNTLFKVDNH TYVQLDRPHT
260 270 280 290 300
PQFLVQLNET LRRNNRLSNS TGRLTWTLDP KIEPDVGEWA FWETKKTFPN
310 320 330 340 350
NFMEKTCISK FYQPTPTTPQ IRARRELSKE KLATTHPPTT PSWFQRIPLQ
360
WFQCSLQDGQ RKCRPKV
Length:367
Mass (Da):41,771
Last modified:November 1, 1996 - v1
Checksum:iF3953243F5420C40
GO

RNA editingi

Partially edited. RNA editing at this position consists of an insertion of one or two adenine nucleotides. The sequence displayed here is the full-length transmembrane glycoprotein GP, derived from the +1A edited RNA. The unedited RNA gives rise to the small secreted glycoprotein sGP (AC Q66799), the +2A edited RNA gives rise to the super small secreted glycoprotein ssGP (AC P0C771).

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti312 – 3121Y → H.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U23152 Genomic RNA. Translation: AAC54884.1.
AF034645 Genomic RNA. Translation: AAC24345.1.
AF522874 Genomic RNA. Translation: AAN04451.1.
AY769362 Genomic RNA. Translation: AAV48578.1.
RefSeqiNP_690584.1. NC_004161.1.

Genome annotation databases

GeneIDi955190.

Keywords - Coding sequence diversityi

RNA editing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U23152 Genomic RNA. Translation: AAC54884.1 .
AF034645 Genomic RNA. Translation: AAC24345.1 .
AF522874 Genomic RNA. Translation: AAN04451.1 .
AY769362 Genomic RNA. Translation: AAV48578.1 .
RefSeqi NP_690584.1. NC_004161.1.

3D structure databases

ProteinModelPortali Q66800.
SMRi Q66800. Positions 33-285.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 955190.

Family and domain databases

InterProi IPR014625. GPC_FiloV.
IPR002561. GPC_filovir-type_extra_dom.
[Graphical view ]
Pfami PF01611. Filo_glycop. 1 hit.
[Graphical view ]
PIRSFi PIRSF036874. GPC_FiloV. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The virion glycoproteins of Ebola viruses are encoded in two reading frames and are expressed through transcriptional editing."
    Sanchez A., Trappier S.G., Mahy B.W.J., Peters C.J., Nichol S.T.
    Proc. Natl. Acad. Sci. U.S.A. 93:3602-3607(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA], RNA EDITING.
  2. Volchkov V.E.
    Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  3. "Molecular characterization of an isolate from the 1989/90 epizootic of Ebola virus Reston among macaques imported into the United States."
    Groseth A., Stroeher U., Theriault S., Feldmann H.
    Virus Res. 87:155-163(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  4. "A reconstituted replication and transcription system for Ebola virus Reston and comparison with Ebola virus Zaire."
    Boehmann Y., Enterlein S., Randolf A., Muehlberger E.I.
    Virology 332:406-417(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: Isolate Pennsylvania-89.

Entry informationi

Entry nameiVSGP_EBORR
AccessioniPrimary (citable) accession number: Q66800
Secondary accession number(s): Q5UAK7, Q8JPX7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3