Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q66790

- POLG_EC16H

UniProt

Q66790 - POLG_EC16H

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Genome polyprotein

Gene
N/A
Organism
Echovirus 16 (strain Harrington)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Protein VP1: Forms, together with VP2 and VP3, an icosahedral capsid (pseudo T=3), 300 Angstroms in diameter, composed of 60 copies of each capsid protein and enclosing the viral positive strand RNA genome. Protein VP1 mainly forms the vertices of the capsid. VP1 interacts with host cell receptor to provide virion attachment to target cell. After binding to its receptor, the capsid undergoes conformational changes. VP1 N-terminus (that contains an amphipathic alpha-helix) is externalized, VP4 is released and together, they shape a virion-cell connecting channel and a pore in the host membrane through which RNase-protected transfer of the viral genome takes place. After genome has been released, the channel shrinks (By similarity).By similarity
Protein VP2: Forms, together with VP1 and VP3, an icosahedral capsid (pseudo T=3), 300 Angstroms in diameter, composed of 60 copies of each capsid protein and enclosing the viral positive strand RNA genome.By similarity
Protein VP3: Forms, together with VP1 and VP2, an icosahedral capsid (pseudo T=3), 300 Angstroms in diameter, composed of 60 copies of each capsid protein and enclosing the viral positive strand RNA genome.By similarity
Protein VP4: Lies on the inner surface of the capsid shell. After binding to the host receptor, the capsid undergoes conformational changes. VP4 is released, VP1 N-terminus is externalized, and together, they shape a virion-cell connecting channel and a pore in the host membrane through which RNase-protected transfer of the viral genome takes place. After genome has been released, the channel shrinks (By similarity).By similarity
Protein VP0: Protein VP0: VP0 precursor is a component of immature procapsids, which gives rise to VP4 and VP2 after maturation. Allows the capsid to remain inactive before the maturation step (By similarity).By similarity
Protease 2A: cysteine protease that is responsible for the cleavage between the P1 and P2 regions. It cleaves the host translation initiation factor EIF4G1, in order to shut off the capped cellular mRNA transcription (By similarity).By similarity

Catalytic activityi

Selective cleavage of Tyr-|-Gly bond in the picornavirus polyprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei69 – 702CleavageSequence Analysis
Sitei331 – 3322Cleavage; by protease 3CSequence Analysis
Sitei854 – 8552Cleavage; by protease 2ASequence Analysis

GO - Molecular functioni

  1. cysteine-type peptidase activity Source: UniProtKB-KW
  2. structural molecule activity Source: InterPro
  3. transferase activity Source: UniProtKB-KW

GO - Biological processi

  1. pore-mediated entry of viral genome into host cell Source: UniProtKB-KW
  2. suppression by virus of host gene expression Source: UniProtKB-KW
  3. virion attachment to host cell Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease, Transferase

Keywords - Biological processi

Eukaryotic host gene expression shutoff by virus, Eukaryotic host translation shutoff by virus, Host gene expression shutoff by virus, Host-virus interaction, Pore-mediated penetration of viral genome into host cell, Viral attachment to host cell, Viral penetration into host cytoplasm, Virus entry into host cell

Protein family/group databases

MEROPSiN08.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Genome polyprotein
Cleaved into the following 6 chains:
Alternative name(s):
VP4-VP2
Alternative name(s):
P1A
Virion protein 4
Alternative name(s):
P1B
Virion protein 2
Alternative name(s):
P1C
Virion protein 3
Alternative name(s):
P1D
Virion protein 1
Protease 2A (EC:3.4.22.29)
Short name:
P2A
Alternative name(s):
Protein 2A
OrganismiEchovirus 16 (strain Harrington)
Taxonomic identifieri103910 [NCBI]
Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stagePicornaviralesPicornaviridaeEnterovirusEnterovirus B
Virus hostiHomo sapiens (Human) [TaxID: 9606]

Subcellular locationi

GO - Cellular componenti

  1. host cell cytoplasm Source: UniProtKB-KW
  2. viral capsid Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Host cytoplasm, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed; by hostBy similarity
Chaini2 – 331330Protein VP0Sequence AnalysisPRO_0000311060Add
BLAST
Chaini2 – 6968Protein VP4Sequence AnalysisPRO_0000039725Add
BLAST
Chaini70 – 331262Protein VP2Sequence AnalysisPRO_0000039726Add
BLAST
Chaini332 – 570239Protein VP3Sequence AnalysisPRO_0000039727Add
BLAST
Chaini571 – 854284Protein VP1Sequence AnalysisPRO_0000039728Add
BLAST
Chaini855 – ›862›8Protease 2ASequence AnalysisPRO_0000039729

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycine; by hostBy similarity

Post-translational modificationi

Specific enzymatic cleavages in vivo by the viral proteases yield a variety of precursors and mature proteins. Polyprotein processing intermediates such as VP0 which is a VP4-VP2 precursor are produced. During virion maturation, non-infectious particles are rendered infectious following cleavage of VP0. This maturation cleavage is followed by a conformational change of the particle (By similarity).By similarity
Myristoylation of VP4 is required during RNA encapsidation and formation of the mature virus particle.By similarity

Keywords - PTMi

Lipoprotein, Myristate

Structurei

3D structure databases

ProteinModelPortaliQ66790.
SMRiQ66790. Positions 2-69, 79-328, 332-853.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the picornaviruses polyprotein family.Curated
Contains 1 peptidase C3 domain.Curated

Family and domain databases

Gene3Di2.60.120.20. 3 hits.
4.10.80.10. 2 hits.
InterProiIPR003138. Pico_P1A.
IPR001676. Picornavirus_capsid.
IPR029053. Viral_coat.
[Graphical view]
PfamiPF02226. Pico_P1A. 1 hit.
PF00073. Rhv. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q66790-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGAQVSTQKT GAHETLLEAA QGATINYTNI NYYKDAASNS ANRQDFSQDP
60 70 80 90 100
SKFTEPVKDI MIKSMPALNS PSAEECGYSD RVRSITLGNS TITTQESANV
110 120 130 140 150
VVAYGRWPKY LEDDQATAED QPTQPDVATC RFYTLESVQW EANSAGWWWK
160 170 180 190 200
FPEALKDMGL FGQNMYYHYL GRAGYTIHVQ CNASKFHQGC LLVVCVPEAE
210 220 230 240 250
MGCAKPDENV DATNLTNGEN TCELTAGAAP AEKGKVQTAV CNATMGVAVG
260 270 280 290 300
NLTIFPHQWI NLRTNNCATI VMPYINSVPM DNMFRHYNFT LMVIPFVPLT
310 320 330 340 350
SMGGSTYVPI TVTIAPMCAE YNGLRLSTQH QGLPVMNVPG SNQFLTSDNF
360 370 380 390 400
QSPCAMPEYD VTPPLDIPGE VNNLMEVAEV DSVVPVNNLS DNVKTIKAYQ
410 420 430 440 450
IPVSAGDSSR PEAVFKFQLD PGSGSVLKHT LLGEIINYYA HWSGSIKLTF
460 470 480 490 500
VFCGSAMATG KLLIAYSPPG ASAPATRKDA MLGTHIIWDL GLQSSCVLCV
510 520 530 540 550
PWISQTHYRM VERDEYTTAG YISCWYQTNI IVPPDTPSQC YMLCLASACN
560 570 580 590 600
DFSVRMLKDT PFIQQEAKLQ GEPGKAIESA ISRVADTISS GPTNSEQVPA
610 620 630 640 650
LTAAETGHTS QVVPGDTIQT RHVKNYHSRS ESTIENFLCR SACVHIARYE
660 670 680 690 700
AGANASNEDR FVRWEINNKE LVQLRRKCEM FTYLRYDVEV TFVITSQQDQ
710 720 730 740 750
GTDLSQDMPV LTHQVMYVPP GGSVTKQGDS YAWQTSTNPS VFWTEGNAPP
760 770 780 790 800
RMSIPFISIG NAYSSFYDGW SHFSQKGVYG YNTLNKMGTL FVRHVNKETP
810 820 830 840 850
KPVTSTVRVY FKPKHIRAWI PRPPRLCPYK YKANVNFDVT AITDSRLTIT
860
TVPQVEHNLR TA
Length:862
Mass (Da):95,399
Last modified:January 23, 2007 - v3
Checksum:i59587E197126595C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei862 – 8621

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X89545 Genomic RNA. Translation: CAA61723.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X89545 Genomic RNA. Translation: CAA61723.1 .

3D structure databases

ProteinModelPortali Q66790.
SMRi Q66790. Positions 2-69, 79-328, 332-853.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi N08.001.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 2.60.120.20. 3 hits.
4.10.80.10. 2 hits.
InterProi IPR003138. Pico_P1A.
IPR001676. Picornavirus_capsid.
IPR029053. Viral_coat.
[Graphical view ]
Pfami PF02226. Pico_P1A. 1 hit.
PF00073. Rhv. 3 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The major echovirus group is genetically coherent and related to coxsackie B viruses."
    Huttunen P., Santti J., Pulli T., Hyypiae T.
    J. Gen. Virol. 77:715-725(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiPOLG_EC16H
AccessioniPrimary (citable) accession number: Q66790
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 88 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3