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Q66666 (DEN_EHV2) Reviewed, UniProtKB/Swiss-Prot

Last modified July 24, 2013. Version 36. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Deneddylase

EC=3.4.19.12
EC=3.4.22.-
Alternative name(s):
Ubiquitin thioesterase
Gene names
Name:64
OrganismEquine herpesvirus 2 (strain 86/87) (EHV-2) [Reference proteome]
Taxonomic identifier82831 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeGammaherpesvirinaePercavirus
Virus hostEquus caballus (Horse) [TaxID: 9796]

Protein attributes

Sequence length3436 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Deneddylase that deregulates the host cell cycle S phase to create a favorable environment allowing efficient viral genome replication. Interacts with and deneddylates host cullins including CUL1 and CUL4A, thereby reducing their E3 ubiquitin ligase activity. Inhibition of cullins leads to the stabilization of cullin-RING ligase substrates such as host CDN1A/p21, CDKN1B/p27kip and CDC25A, preventing S phase progression. Additionally, acts as a deubiquitinase and cleaves both 'Lys-48' and 'Lys-63'-linked ubiquitin chains By similarity.

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Subunit structure

Interacts with host CUL1 and CUL4A; these interactions inhibit the E3 ligase activity of cullins By similarity.

Subcellular location

Virion tegument. Host cytoplasm. Host nucleus By similarity. Note: Tightly associated with the capsid By similarity.

Sequence similarities

Belongs to the herpesviridae deneddylase family.

Contains 1 peptidase C76 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 34363436Deneddylase
PRO_0000406058

Regions

Domain27 – 241215Peptidase C76
Coiled coil1761 – 179535 Potential
Coiled coil2038 – 207538 Potential
Coiled coil2177 – 224670 Potential
Coiled coil2296 – 232631 Potential
Compositional bias710 – 1249540Gly-rich
Compositional bias1369 – 13724Poly-Ser
Compositional bias2577 – 2793217Gly-rich
Compositional bias3147 – 319145Glu-rich

Sites

Active site471 By similarity
Active site1791 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q66666 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 663BBC19D77EF818

FASTA3,436367,372
        10         20         30         40         50         60 
MEGIKIDELD LKTQAIFLHY QKFTLEGLAS NHQGVACKFG RYAGMQCLSN CTAYLLYTYY 

        70         80         90        100        110        120 
NGGVPLKDRT ELDHVLNVGS KIDFVLRQGG SIAPDQYVQI AEIPGTVITS QVNCHIYQSN 

       130        140        150        160        170        180 
EIFGLLGVDA PVIHDDVRSL KHVLTRNYNR VTQYMICICN AMALAVVIKD KRYYLFNPHC 

       190        200        210        220        230        240 
VPGIANSVAH VISTPDADDL VSYVGPTGVE YTGAFLYFVP AEVDIGNHAA FIANHYKVLR 

       250        260        270        280        290        300 
FNRPNAIQVD VSEADGGGAC IRELPKDSSD GAPADGNSAP PLESKRTRSA ESAKEVGQKL 

       310        320        330        340        350        360 
VKRRQKKGFA PYTKSKPPNG RDEAAADEVA RVLPNTGATP ERTVSPGVAP MSEDRPASED 

       370        380        390        400        410        420 
FYPDSEHPDS DFSDTDPDLA QSQLDESLLA RDQSLEPNAD VEFGAEGAPR TAPETPVVGD 

       430        440        450        460        470        480 
GNSSFDSFHL PTTRDPGERC GRVTAADEHA VGGASLGVES LQPPTTLAPD ERSRGRSGDG 

       490        500        510        520        530        540 
RGGPSAADDR VVGVVNSSDN SFQTPTTPAP DESRRLSRLE GEGGDAGTAS EDVAVPGGDS 

       550        560        570        580        590        600 
RRAGEKVTHP HTGPSARGSR SPEGVPGAED AGRGASPGHR VPVSARSSQA QQAVSGPNSQ 

       610        620        630        640        650        660 
AEGDEGLPPR DTDGRGKAAR RSRPGGDGAP AFNLWHGDVN SHKESPPPRP SEKTGEDPSM 

       670        680        690        700        710        720 
VKTAKGDMSP LYGDPSTGTL MHKCSDRPTP ESIHACTREL DENMVLDEDG GSGCGDGRGD 

       730        740        750        760        770        780 
VRSGGESAPS DRNSLSESAS LGARPPPERR LPETREDWEG EGDAMMAIEA GPEGDSAKRP 

       790        800        810        820        830        840 
VYGKVSADGG SSFPDAGASA RKTGPRAAKK KGGEKKRKGR GVEGGSIPAS SPGSEVGCEG 

       850        860        870        880        890        900 
GARSPTAHPA QLAQREEGVS DGGDGYSSAD SLVTGYEGNY RERSHPVTSL PTEDEDMAFE 

       910        920        930        940        950        960 
EEGEREGGAA AEETSDARGA QGTGDFNSLK QPKTPLSCLP LDQKPGSRVR GKKGRNRGGA 

       970        980        990       1000       1010       1020 
AGEERVVAGG KLDAGYTDPG DVTVFPASSF GPCETVSLGA SGSAHARQEG GGNEELPSDE 

      1030       1040       1050       1060       1070       1080 
SIGSECLSRS ESLKHSGSYR PSERARGGRG SVEDGEGSYD GNFPGEGPRR PGTTDLSEGY 

      1090       1100       1110       1120       1130       1140 
LVPRRQFLSR DRRRGGEEGG GEDDGMSAED GLPPDEKWLP MTAELSGDSA VRDNHFSDGD 

      1150       1160       1170       1180       1190       1200 
ERRGARGNGG EGWGDDTLRT DSWRNLKTTD LGEDSVVSGS RYPSMEQQSV GEGGRDGLPA 

      1210       1220       1230       1240       1250       1260 
DGKREPKAAD RGSGSTAPGS RVPSAGREPG GQCEGEDHVA MDEGAPGPGS PLVKKKQDND 

      1270       1280       1290       1300       1310       1320 
TLFPHRGDHR APEPANPVSL LVLANPPSAP VSRAAEDMLT DMASLKRKRS RAGTASDSED 

      1330       1340       1350       1360       1370       1380 
DAGPNDYHAH QSKSANWGDA LDDDIWIDDV PEPARSPESP DLGSERLFSS SSEESLHTAD 

      1390       1400       1410       1420       1430       1440 
EGGDDGAGEG GAGDEEGSGL EEDDEFEGEG ARGATAQGQG VDFSEVDTFI GNMRQFSHRE 

      1450       1460       1470       1480       1490       1500 
SLPLIVDASI SRGFREANAL HTIDKLITNI IIESGVVTGP AHPSKAKNLL RFIVLWGRKL 

      1510       1520       1530       1540       1550       1560 
GIPTRELEIF METELEISRL CSAIDDGAFP EGLFLSHVLT KVNRCLPAIY TQTRGIVKKI 

      1570       1580       1590       1600       1610       1620 
ASILTAETQK VEGRETVADP RGYAGILTEA FDKDAYIICT KGESVEIVKK IAALREAVAA 

      1630       1640       1650       1660       1670       1680 
RNAEIARESN SFEALMGALR AYGPVPEGVG PMESQPGVKD RAFAEAVGEV MGDLTERVRN 

      1690       1700       1710       1720       1730       1740 
VTSEFTDSLA AGHMDTTYLP DLETLIQNID STIRLLGFAA RELRMEKKPL MAAAQQLTYM 

      1750       1760       1770       1780       1790       1800 
GGELAAIANA DWAHRPAEPV TQVKEINDLR SRLAQLQKED ENAQATERIL ADVEGMLDDI 

      1810       1820       1830       1840       1850       1860 
TSQRGEGGAV SRTLTVPMLE NYLKNAGALI GESHNEKYLR LKERLRELAS SEDFLVNLIN 

      1870       1880       1890       1900       1910       1920 
TTTLFSVTQT LAKIEQILGG SPHLATGEKV RQAFEGAGEQ IIADAMEAVA ARDPARLDVA 

      1930       1940       1950       1960       1970       1980 
ATGSLPRFLR HSPAPGAEDT GAALFALSEA VKLEKGSKGR IRWRGVEGKL SDAHSLVARS 

      1990       2000       2010       2020       2030       2040 
AAKGAQKRKL FALIQGLRKD YAAQEQREIM EDWKAFVTEA PINSMEDVND ILRAAPNEEA 

      2050       2060       2070       2080       2090       2100 
REFAAKKLEE RVREMREAEA KLEKEAEEML TQAVKRRGLQ CWGRIQNAFD NMAFGGITGE 

      2110       2120       2130       2140       2150       2160 
DWAAVAAEFQ REGSTLSSTL PGQLSKLTDK VEAEVEALLL NKVVSMLPNG PAFKPPAFDW 

      2170       2180       2190       2200       2210       2220 
LTPYRAHMNF YLKSFPLPKL NRQAEAVEAK MSQIEQAIEG ADVYEAVAGT PLEAPVARAL 

      2230       2240       2250       2260       2270       2280 
RLLRAARDEA AGLKGQIDEG ERAYVQGVER AGEGGEPPAK PKAEIPKKLL TYEQTLSLAN 

      2290       2300       2310       2320       2330       2340 
LPEDFQKNVL QNETLMLNQL REYLGRVTEN INSLESRAKT SRGEANARLA AVIEENLPQA 

      2350       2360       2370       2380       2390       2400 
NVSISSRRLD KSDPVGFLEG IVRDKQIVES DPYSATRESL VWLHRTFKAL LPLCPASLKR 

      2410       2420       2430       2440       2450       2460 
RMELLGEEIL REKGRGRGAP RVGVRAHETD DVGVLTEAIE TLEARRVTGG KAAVEGWVKK 

      2470       2480       2490       2500       2510       2520 
RDAYRNMVED LALRSEVEKR LGPLVEKSRE ALDAPDLAVL QEEAAALLLE AKTGGLDKSA 

      2530       2540       2550       2560       2570       2580 
PETHERVLEL QMYLRFKLDF LKHYLDSQRP VFEAAPLSRA LYWSNSQGER ESRENSGEGE 

      2590       2600       2610       2620       2630       2640 
GVEGGERRGE GGGEGSPSGA PLRLAPRLSI YRSLARQATK FPGPVAGDQA LRGPGPRDPG 

      2650       2660       2670       2680       2690       2700 
AQRAGGPPST CSPSSPTSWS CCSSSPRRTR GWGPAGGPVR DSGQEAGGGA AGHAGQPVGG 

      2710       2720       2730       2740       2750       2760 
HSGPRAGRPG RLPRQQDRVR AGQPLLRHDP LRPRGQRGRP GAGRGAEKPR GRGPARDPPP 

      2770       2780       2790       2800       2810       2820 
VGAAAAGHPA RAGGRLPARP LPEGGGGGAQ DRGPLPAALT PTLFLEDMYF PRSRPEVDHL 

      2830       2840       2850       2860       2870       2880 
KALPPPRAFL FRPGRWPAAD VTQALWGSDG FAPLFPPKED GRARAGFLLW ALLTVDHVVL 

      2890       2900       2910       2920       2930       2940 
GQLWSTLPPL DMGLKSPLHL LYALAAAEYP NTVPDAERES GGEPGTPYPY GPPTGSYYTL 

      2950       2960       2970       2980       2990       3000 
GPTEAGGAEK VPVTGFEVAV GCLLRGVSAH IFIDSERPWA PKTNSGGEID LVAPVLDCTG 

      3010       3020       3030       3040       3050       3060 
AVDPFKSLKA VPRKTLSPSA ELFDGLAGRE EVEVFGRQAA WLGERKRGGR KRDRFIVVLD 

      3070       3080       3090       3100       3110       3120 
PNNYLKGSYL GNARPPGDAP PVELAIEENK NWPAEALAPN LQAASPIPKS RVAAEYKKLT 

      3130       3140       3150       3160       3170       3180 
EKYMDLALKQ VFAEFPGSVH DPPDSREEEE GEGEAGQGAE EWQEEEWEER EEPRPPRRLG 

      3190       3200       3210       3220       3230       3240 
ERGRREREVV EAVAPAPRDY SRPRVPPRRE PSGGPRSPAR DRDTLGGGAG SREKVSEGRG 

      3250       3260       3270       3280       3290       3300 
RPRVQLSRSP KPRPAASQVQ GPREEVGFSP GRARRGGSTA HAPPETDTAD YIEPPKSGVG 

      3310       3320       3330       3340       3350       3360 
AGSGPPEKKQ QGAAEAEAPA PREGEGAAQE APGEGTPEPA SIGKTSKGKK KPLFTKLPPS 

      3370       3380       3390       3400       3410       3420 
SVDLKDLFKK PGLGIYGGLT SPRFSDESES AGEGKGESES EPESPNTTAL PASGETSRSL 

      3430 
SRRQVLLSKT QTRLHF 

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References

[1]"The DNA sequence of equine herpesvirus 2."
Telford E.A.R., Watson M.S., Aird H.C., Perry J., Davison A.J.
J. Mol. Biol. 249:520-528(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U20824 Genomic DNA. Translation: AAC13852.1.
PIRS55659.
RefSeqNP_042661.1. NC_001650.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1461045.

Family and domain databases

InterProIPR006928. Pept_C76_UL36-USP.
[Graphical view]
PfamPF04843. Herpes_teg_N. 1 hit.
[Graphical view]
PROSITEPS51521. HTUSP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDEN_EHV2
AccessionPrimary (citable) accession number: Q66666
Entry history
Integrated into UniProtKB/Swiss-Prot: March 8, 2011
Last sequence update: November 1, 1996
Last modified: July 24, 2013
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries