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Q66666

- DEN_EHV2

UniProt

Q66666 - DEN_EHV2

Protein

Deneddylase

Gene

64

Organism
Equine herpesvirus 2 (strain 86/87) (EHV-2)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 38 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Deneddylase that deregulates the host cell cycle S phase to create a favorable environment allowing efficient viral genome replication. Interacts with and deneddylates host cullins including CUL1 and CUL4A, thereby reducing their E3 ubiquitin ligase activity. Inhibition of cullins leads to the stabilization of cullin-RING ligase substrates such as host CDN1A/p21, CDKN1B/p27kip and CDC25A, preventing S phase progression. Additionally, acts as a deubiquitinase and cleaves both 'Lys-48' and 'Lys-63'-linked ubiquitin chains By similarity.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei47 – 471PROSITE-ProRule annotation
    Active sitei179 – 1791PROSITE-ProRule annotation

    GO - Molecular functioni

    1. cysteine-type peptidase activity Source: UniProtKB-KW

    GO - Biological processi

    1. modulation by virus of host protein ubiquitination Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Host-virus interaction, Modulation of host ubiquitin pathway by viral deubiquitinase, Modulation of host ubiquitin pathway by virus, Ubl conjugation pathway

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Deneddylase (EC:3.4.19.12, EC:3.4.22.-)
    Alternative name(s):
    Ubiquitin thioesterase
    Gene namesi
    Name:64
    OrganismiEquine herpesvirus 2 (strain 86/87) (EHV-2)
    Taxonomic identifieri82831 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeGammaherpesvirinaePercavirus
    Virus hostiEquus caballus (Horse) [TaxID: 9796]
    ProteomesiUP000007083: Genome

    Subcellular locationi

    Virion tegument. Host cytoplasm. Host nucleus By similarity
    Note: Tightly associated with the capsid.By similarity

    GO - Cellular componenti

    1. host cell cytoplasm Source: UniProtKB-SubCell
    2. host cell nucleus Source: UniProtKB-SubCell
    3. viral tegument Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host cytoplasm, Host nucleus, Virion, Virion tegument

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 34363436DeneddylasePRO_0000406058Add
    BLAST

    Interactioni

    Subunit structurei

    Interacts with host CUL1 and CUL4A; these interactions inhibit the E3 ligase activity of cullins.By similarity

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini27 – 241215Peptidase C76PROSITE-ProRule annotationAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili1761 – 179535Sequence AnalysisAdd
    BLAST
    Coiled coili2038 – 207538Sequence AnalysisAdd
    BLAST
    Coiled coili2177 – 224670Sequence AnalysisAdd
    BLAST
    Coiled coili2296 – 232631Sequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi710 – 1249540Gly-richAdd
    BLAST
    Compositional biasi1369 – 13724Poly-Ser
    Compositional biasi2577 – 2793217Gly-richAdd
    BLAST
    Compositional biasi3147 – 319145Glu-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the herpesviridae deneddylase family.Curated
    Contains 1 peptidase C76 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil, Repeat

    Family and domain databases

    InterProiIPR006928. Pept_C76_UL36-USP.
    [Graphical view]
    PfamiPF04843. Herpes_teg_N. 1 hit.
    [Graphical view]
    PROSITEiPS51521. HTUSP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q66666-1 [UniParc]FASTAAdd to Basket

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    MEGIKIDELD LKTQAIFLHY QKFTLEGLAS NHQGVACKFG RYAGMQCLSN     50
    CTAYLLYTYY NGGVPLKDRT ELDHVLNVGS KIDFVLRQGG SIAPDQYVQI 100
    AEIPGTVITS QVNCHIYQSN EIFGLLGVDA PVIHDDVRSL KHVLTRNYNR 150
    VTQYMICICN AMALAVVIKD KRYYLFNPHC VPGIANSVAH VISTPDADDL 200
    VSYVGPTGVE YTGAFLYFVP AEVDIGNHAA FIANHYKVLR FNRPNAIQVD 250
    VSEADGGGAC IRELPKDSSD GAPADGNSAP PLESKRTRSA ESAKEVGQKL 300
    VKRRQKKGFA PYTKSKPPNG RDEAAADEVA RVLPNTGATP ERTVSPGVAP 350
    MSEDRPASED FYPDSEHPDS DFSDTDPDLA QSQLDESLLA RDQSLEPNAD 400
    VEFGAEGAPR TAPETPVVGD GNSSFDSFHL PTTRDPGERC GRVTAADEHA 450
    VGGASLGVES LQPPTTLAPD ERSRGRSGDG RGGPSAADDR VVGVVNSSDN 500
    SFQTPTTPAP DESRRLSRLE GEGGDAGTAS EDVAVPGGDS RRAGEKVTHP 550
    HTGPSARGSR SPEGVPGAED AGRGASPGHR VPVSARSSQA QQAVSGPNSQ 600
    AEGDEGLPPR DTDGRGKAAR RSRPGGDGAP AFNLWHGDVN SHKESPPPRP 650
    SEKTGEDPSM VKTAKGDMSP LYGDPSTGTL MHKCSDRPTP ESIHACTREL 700
    DENMVLDEDG GSGCGDGRGD VRSGGESAPS DRNSLSESAS LGARPPPERR 750
    LPETREDWEG EGDAMMAIEA GPEGDSAKRP VYGKVSADGG SSFPDAGASA 800
    RKTGPRAAKK KGGEKKRKGR GVEGGSIPAS SPGSEVGCEG GARSPTAHPA 850
    QLAQREEGVS DGGDGYSSAD SLVTGYEGNY RERSHPVTSL PTEDEDMAFE 900
    EEGEREGGAA AEETSDARGA QGTGDFNSLK QPKTPLSCLP LDQKPGSRVR 950
    GKKGRNRGGA AGEERVVAGG KLDAGYTDPG DVTVFPASSF GPCETVSLGA 1000
    SGSAHARQEG GGNEELPSDE SIGSECLSRS ESLKHSGSYR PSERARGGRG 1050
    SVEDGEGSYD GNFPGEGPRR PGTTDLSEGY LVPRRQFLSR DRRRGGEEGG 1100
    GEDDGMSAED GLPPDEKWLP MTAELSGDSA VRDNHFSDGD ERRGARGNGG 1150
    EGWGDDTLRT DSWRNLKTTD LGEDSVVSGS RYPSMEQQSV GEGGRDGLPA 1200
    DGKREPKAAD RGSGSTAPGS RVPSAGREPG GQCEGEDHVA MDEGAPGPGS 1250
    PLVKKKQDND TLFPHRGDHR APEPANPVSL LVLANPPSAP VSRAAEDMLT 1300
    DMASLKRKRS RAGTASDSED DAGPNDYHAH QSKSANWGDA LDDDIWIDDV 1350
    PEPARSPESP DLGSERLFSS SSEESLHTAD EGGDDGAGEG GAGDEEGSGL 1400
    EEDDEFEGEG ARGATAQGQG VDFSEVDTFI GNMRQFSHRE SLPLIVDASI 1450
    SRGFREANAL HTIDKLITNI IIESGVVTGP AHPSKAKNLL RFIVLWGRKL 1500
    GIPTRELEIF METELEISRL CSAIDDGAFP EGLFLSHVLT KVNRCLPAIY 1550
    TQTRGIVKKI ASILTAETQK VEGRETVADP RGYAGILTEA FDKDAYIICT 1600
    KGESVEIVKK IAALREAVAA RNAEIARESN SFEALMGALR AYGPVPEGVG 1650
    PMESQPGVKD RAFAEAVGEV MGDLTERVRN VTSEFTDSLA AGHMDTTYLP 1700
    DLETLIQNID STIRLLGFAA RELRMEKKPL MAAAQQLTYM GGELAAIANA 1750
    DWAHRPAEPV TQVKEINDLR SRLAQLQKED ENAQATERIL ADVEGMLDDI 1800
    TSQRGEGGAV SRTLTVPMLE NYLKNAGALI GESHNEKYLR LKERLRELAS 1850
    SEDFLVNLIN TTTLFSVTQT LAKIEQILGG SPHLATGEKV RQAFEGAGEQ 1900
    IIADAMEAVA ARDPARLDVA ATGSLPRFLR HSPAPGAEDT GAALFALSEA 1950
    VKLEKGSKGR IRWRGVEGKL SDAHSLVARS AAKGAQKRKL FALIQGLRKD 2000
    YAAQEQREIM EDWKAFVTEA PINSMEDVND ILRAAPNEEA REFAAKKLEE 2050
    RVREMREAEA KLEKEAEEML TQAVKRRGLQ CWGRIQNAFD NMAFGGITGE 2100
    DWAAVAAEFQ REGSTLSSTL PGQLSKLTDK VEAEVEALLL NKVVSMLPNG 2150
    PAFKPPAFDW LTPYRAHMNF YLKSFPLPKL NRQAEAVEAK MSQIEQAIEG 2200
    ADVYEAVAGT PLEAPVARAL RLLRAARDEA AGLKGQIDEG ERAYVQGVER 2250
    AGEGGEPPAK PKAEIPKKLL TYEQTLSLAN LPEDFQKNVL QNETLMLNQL 2300
    REYLGRVTEN INSLESRAKT SRGEANARLA AVIEENLPQA NVSISSRRLD 2350
    KSDPVGFLEG IVRDKQIVES DPYSATRESL VWLHRTFKAL LPLCPASLKR 2400
    RMELLGEEIL REKGRGRGAP RVGVRAHETD DVGVLTEAIE TLEARRVTGG 2450
    KAAVEGWVKK RDAYRNMVED LALRSEVEKR LGPLVEKSRE ALDAPDLAVL 2500
    QEEAAALLLE AKTGGLDKSA PETHERVLEL QMYLRFKLDF LKHYLDSQRP 2550
    VFEAAPLSRA LYWSNSQGER ESRENSGEGE GVEGGERRGE GGGEGSPSGA 2600
    PLRLAPRLSI YRSLARQATK FPGPVAGDQA LRGPGPRDPG AQRAGGPPST 2650
    CSPSSPTSWS CCSSSPRRTR GWGPAGGPVR DSGQEAGGGA AGHAGQPVGG 2700
    HSGPRAGRPG RLPRQQDRVR AGQPLLRHDP LRPRGQRGRP GAGRGAEKPR 2750
    GRGPARDPPP VGAAAAGHPA RAGGRLPARP LPEGGGGGAQ DRGPLPAALT 2800
    PTLFLEDMYF PRSRPEVDHL KALPPPRAFL FRPGRWPAAD VTQALWGSDG 2850
    FAPLFPPKED GRARAGFLLW ALLTVDHVVL GQLWSTLPPL DMGLKSPLHL 2900
    LYALAAAEYP NTVPDAERES GGEPGTPYPY GPPTGSYYTL GPTEAGGAEK 2950
    VPVTGFEVAV GCLLRGVSAH IFIDSERPWA PKTNSGGEID LVAPVLDCTG 3000
    AVDPFKSLKA VPRKTLSPSA ELFDGLAGRE EVEVFGRQAA WLGERKRGGR 3050
    KRDRFIVVLD PNNYLKGSYL GNARPPGDAP PVELAIEENK NWPAEALAPN 3100
    LQAASPIPKS RVAAEYKKLT EKYMDLALKQ VFAEFPGSVH DPPDSREEEE 3150
    GEGEAGQGAE EWQEEEWEER EEPRPPRRLG ERGRREREVV EAVAPAPRDY 3200
    SRPRVPPRRE PSGGPRSPAR DRDTLGGGAG SREKVSEGRG RPRVQLSRSP 3250
    KPRPAASQVQ GPREEVGFSP GRARRGGSTA HAPPETDTAD YIEPPKSGVG 3300
    AGSGPPEKKQ QGAAEAEAPA PREGEGAAQE APGEGTPEPA SIGKTSKGKK 3350
    KPLFTKLPPS SVDLKDLFKK PGLGIYGGLT SPRFSDESES AGEGKGESES 3400
    EPESPNTTAL PASGETSRSL SRRQVLLSKT QTRLHF 3436
    Length:3,436
    Mass (Da):367,372
    Last modified:November 1, 1996 - v1
    Checksum:i663BBC19D77EF818
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U20824 Genomic DNA. Translation: AAC13852.1.
    PIRiS55659.
    RefSeqiNP_042661.1. NC_001650.1.

    Genome annotation databases

    GeneIDi1461045.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U20824 Genomic DNA. Translation: AAC13852.1 .
    PIRi S55659.
    RefSeqi NP_042661.1. NC_001650.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 1461045.

    Family and domain databases

    InterProi IPR006928. Pept_C76_UL36-USP.
    [Graphical view ]
    Pfami PF04843. Herpes_teg_N. 1 hit.
    [Graphical view ]
    PROSITEi PS51521. HTUSP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

    Entry informationi

    Entry nameiDEN_EHV2
    AccessioniPrimary (citable) accession number: Q66666
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 8, 2011
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 38 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3