Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q66666

- DEN_EHV2

UniProt

Q66666 - DEN_EHV2

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Deneddylase

Gene

64

Organism
Equine herpesvirus 2 (strain 86/87) (EHV-2)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Deneddylase that deregulates the host cell cycle S phase to create a favorable environment allowing efficient viral genome replication. Interacts with and deneddylates host cullins including CUL1 and CUL4A, thereby reducing their E3 ubiquitin ligase activity. Inhibition of cullins leads to the stabilization of cullin-RING ligase substrates such as host CDN1A/p21, CDKN1B/p27kip and CDC25A, preventing S phase progression. Additionally, acts as a deubiquitinase and cleaves both 'Lys-48' and 'Lys-63'-linked ubiquitin chains (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei47 – 471PROSITE-ProRule annotation
Active sitei179 – 1791PROSITE-ProRule annotation

GO - Molecular functioni

  1. cysteine-type peptidase activity Source: UniProtKB-KW

GO - Biological processi

  1. modulation by virus of host protein ubiquitination Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Host-virus interaction, Modulation of host ubiquitin pathway by viral deubiquitinase, Modulation of host ubiquitin pathway by virus, Ubl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Deneddylase (EC:3.4.19.12, EC:3.4.22.-)
Alternative name(s):
Ubiquitin thioesterase
Gene namesi
Name:64
OrganismiEquine herpesvirus 2 (strain 86/87) (EHV-2)
Taxonomic identifieri82831 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeGammaherpesvirinaePercavirus
Virus hostiEquus caballus (Horse) [TaxID: 9796]
ProteomesiUP000007083: Genome

Subcellular locationi

Virion tegument. Host cytoplasm. Host nucleus By similarity
Note: Tightly associated with the capsid.By similarity

GO - Cellular componenti

  1. host cell cytoplasm Source: UniProtKB-KW
  2. host cell nucleus Source: UniProtKB-KW
  3. viral tegument Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Host nucleus, Virion, Virion tegument

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 34363436DeneddylasePRO_0000406058Add
BLAST

Interactioni

Subunit structurei

Interacts with host CUL1 and CUL4A; these interactions inhibit the E3 ligase activity of cullins.By similarity

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini27 – 241215Peptidase C76PROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili1761 – 179535Sequence AnalysisAdd
BLAST
Coiled coili2038 – 207538Sequence AnalysisAdd
BLAST
Coiled coili2177 – 224670Sequence AnalysisAdd
BLAST
Coiled coili2296 – 232631Sequence AnalysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi710 – 1249540Gly-richAdd
BLAST
Compositional biasi1369 – 13724Poly-Ser
Compositional biasi2577 – 2793217Gly-richAdd
BLAST
Compositional biasi3147 – 319145Glu-richAdd
BLAST

Sequence similaritiesi

Belongs to the herpesviridae deneddylase family.Curated
Contains 1 peptidase C76 domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat

Family and domain databases

InterProiIPR006928. Pept_C76_UL36-USP.
[Graphical view]
PfamiPF04843. Herpes_teg_N. 1 hit.
[Graphical view]
PROSITEiPS51521. HTUSP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q66666-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEGIKIDELD LKTQAIFLHY QKFTLEGLAS NHQGVACKFG RYAGMQCLSN
60 70 80 90 100
CTAYLLYTYY NGGVPLKDRT ELDHVLNVGS KIDFVLRQGG SIAPDQYVQI
110 120 130 140 150
AEIPGTVITS QVNCHIYQSN EIFGLLGVDA PVIHDDVRSL KHVLTRNYNR
160 170 180 190 200
VTQYMICICN AMALAVVIKD KRYYLFNPHC VPGIANSVAH VISTPDADDL
210 220 230 240 250
VSYVGPTGVE YTGAFLYFVP AEVDIGNHAA FIANHYKVLR FNRPNAIQVD
260 270 280 290 300
VSEADGGGAC IRELPKDSSD GAPADGNSAP PLESKRTRSA ESAKEVGQKL
310 320 330 340 350
VKRRQKKGFA PYTKSKPPNG RDEAAADEVA RVLPNTGATP ERTVSPGVAP
360 370 380 390 400
MSEDRPASED FYPDSEHPDS DFSDTDPDLA QSQLDESLLA RDQSLEPNAD
410 420 430 440 450
VEFGAEGAPR TAPETPVVGD GNSSFDSFHL PTTRDPGERC GRVTAADEHA
460 470 480 490 500
VGGASLGVES LQPPTTLAPD ERSRGRSGDG RGGPSAADDR VVGVVNSSDN
510 520 530 540 550
SFQTPTTPAP DESRRLSRLE GEGGDAGTAS EDVAVPGGDS RRAGEKVTHP
560 570 580 590 600
HTGPSARGSR SPEGVPGAED AGRGASPGHR VPVSARSSQA QQAVSGPNSQ
610 620 630 640 650
AEGDEGLPPR DTDGRGKAAR RSRPGGDGAP AFNLWHGDVN SHKESPPPRP
660 670 680 690 700
SEKTGEDPSM VKTAKGDMSP LYGDPSTGTL MHKCSDRPTP ESIHACTREL
710 720 730 740 750
DENMVLDEDG GSGCGDGRGD VRSGGESAPS DRNSLSESAS LGARPPPERR
760 770 780 790 800
LPETREDWEG EGDAMMAIEA GPEGDSAKRP VYGKVSADGG SSFPDAGASA
810 820 830 840 850
RKTGPRAAKK KGGEKKRKGR GVEGGSIPAS SPGSEVGCEG GARSPTAHPA
860 870 880 890 900
QLAQREEGVS DGGDGYSSAD SLVTGYEGNY RERSHPVTSL PTEDEDMAFE
910 920 930 940 950
EEGEREGGAA AEETSDARGA QGTGDFNSLK QPKTPLSCLP LDQKPGSRVR
960 970 980 990 1000
GKKGRNRGGA AGEERVVAGG KLDAGYTDPG DVTVFPASSF GPCETVSLGA
1010 1020 1030 1040 1050
SGSAHARQEG GGNEELPSDE SIGSECLSRS ESLKHSGSYR PSERARGGRG
1060 1070 1080 1090 1100
SVEDGEGSYD GNFPGEGPRR PGTTDLSEGY LVPRRQFLSR DRRRGGEEGG
1110 1120 1130 1140 1150
GEDDGMSAED GLPPDEKWLP MTAELSGDSA VRDNHFSDGD ERRGARGNGG
1160 1170 1180 1190 1200
EGWGDDTLRT DSWRNLKTTD LGEDSVVSGS RYPSMEQQSV GEGGRDGLPA
1210 1220 1230 1240 1250
DGKREPKAAD RGSGSTAPGS RVPSAGREPG GQCEGEDHVA MDEGAPGPGS
1260 1270 1280 1290 1300
PLVKKKQDND TLFPHRGDHR APEPANPVSL LVLANPPSAP VSRAAEDMLT
1310 1320 1330 1340 1350
DMASLKRKRS RAGTASDSED DAGPNDYHAH QSKSANWGDA LDDDIWIDDV
1360 1370 1380 1390 1400
PEPARSPESP DLGSERLFSS SSEESLHTAD EGGDDGAGEG GAGDEEGSGL
1410 1420 1430 1440 1450
EEDDEFEGEG ARGATAQGQG VDFSEVDTFI GNMRQFSHRE SLPLIVDASI
1460 1470 1480 1490 1500
SRGFREANAL HTIDKLITNI IIESGVVTGP AHPSKAKNLL RFIVLWGRKL
1510 1520 1530 1540 1550
GIPTRELEIF METELEISRL CSAIDDGAFP EGLFLSHVLT KVNRCLPAIY
1560 1570 1580 1590 1600
TQTRGIVKKI ASILTAETQK VEGRETVADP RGYAGILTEA FDKDAYIICT
1610 1620 1630 1640 1650
KGESVEIVKK IAALREAVAA RNAEIARESN SFEALMGALR AYGPVPEGVG
1660 1670 1680 1690 1700
PMESQPGVKD RAFAEAVGEV MGDLTERVRN VTSEFTDSLA AGHMDTTYLP
1710 1720 1730 1740 1750
DLETLIQNID STIRLLGFAA RELRMEKKPL MAAAQQLTYM GGELAAIANA
1760 1770 1780 1790 1800
DWAHRPAEPV TQVKEINDLR SRLAQLQKED ENAQATERIL ADVEGMLDDI
1810 1820 1830 1840 1850
TSQRGEGGAV SRTLTVPMLE NYLKNAGALI GESHNEKYLR LKERLRELAS
1860 1870 1880 1890 1900
SEDFLVNLIN TTTLFSVTQT LAKIEQILGG SPHLATGEKV RQAFEGAGEQ
1910 1920 1930 1940 1950
IIADAMEAVA ARDPARLDVA ATGSLPRFLR HSPAPGAEDT GAALFALSEA
1960 1970 1980 1990 2000
VKLEKGSKGR IRWRGVEGKL SDAHSLVARS AAKGAQKRKL FALIQGLRKD
2010 2020 2030 2040 2050
YAAQEQREIM EDWKAFVTEA PINSMEDVND ILRAAPNEEA REFAAKKLEE
2060 2070 2080 2090 2100
RVREMREAEA KLEKEAEEML TQAVKRRGLQ CWGRIQNAFD NMAFGGITGE
2110 2120 2130 2140 2150
DWAAVAAEFQ REGSTLSSTL PGQLSKLTDK VEAEVEALLL NKVVSMLPNG
2160 2170 2180 2190 2200
PAFKPPAFDW LTPYRAHMNF YLKSFPLPKL NRQAEAVEAK MSQIEQAIEG
2210 2220 2230 2240 2250
ADVYEAVAGT PLEAPVARAL RLLRAARDEA AGLKGQIDEG ERAYVQGVER
2260 2270 2280 2290 2300
AGEGGEPPAK PKAEIPKKLL TYEQTLSLAN LPEDFQKNVL QNETLMLNQL
2310 2320 2330 2340 2350
REYLGRVTEN INSLESRAKT SRGEANARLA AVIEENLPQA NVSISSRRLD
2360 2370 2380 2390 2400
KSDPVGFLEG IVRDKQIVES DPYSATRESL VWLHRTFKAL LPLCPASLKR
2410 2420 2430 2440 2450
RMELLGEEIL REKGRGRGAP RVGVRAHETD DVGVLTEAIE TLEARRVTGG
2460 2470 2480 2490 2500
KAAVEGWVKK RDAYRNMVED LALRSEVEKR LGPLVEKSRE ALDAPDLAVL
2510 2520 2530 2540 2550
QEEAAALLLE AKTGGLDKSA PETHERVLEL QMYLRFKLDF LKHYLDSQRP
2560 2570 2580 2590 2600
VFEAAPLSRA LYWSNSQGER ESRENSGEGE GVEGGERRGE GGGEGSPSGA
2610 2620 2630 2640 2650
PLRLAPRLSI YRSLARQATK FPGPVAGDQA LRGPGPRDPG AQRAGGPPST
2660 2670 2680 2690 2700
CSPSSPTSWS CCSSSPRRTR GWGPAGGPVR DSGQEAGGGA AGHAGQPVGG
2710 2720 2730 2740 2750
HSGPRAGRPG RLPRQQDRVR AGQPLLRHDP LRPRGQRGRP GAGRGAEKPR
2760 2770 2780 2790 2800
GRGPARDPPP VGAAAAGHPA RAGGRLPARP LPEGGGGGAQ DRGPLPAALT
2810 2820 2830 2840 2850
PTLFLEDMYF PRSRPEVDHL KALPPPRAFL FRPGRWPAAD VTQALWGSDG
2860 2870 2880 2890 2900
FAPLFPPKED GRARAGFLLW ALLTVDHVVL GQLWSTLPPL DMGLKSPLHL
2910 2920 2930 2940 2950
LYALAAAEYP NTVPDAERES GGEPGTPYPY GPPTGSYYTL GPTEAGGAEK
2960 2970 2980 2990 3000
VPVTGFEVAV GCLLRGVSAH IFIDSERPWA PKTNSGGEID LVAPVLDCTG
3010 3020 3030 3040 3050
AVDPFKSLKA VPRKTLSPSA ELFDGLAGRE EVEVFGRQAA WLGERKRGGR
3060 3070 3080 3090 3100
KRDRFIVVLD PNNYLKGSYL GNARPPGDAP PVELAIEENK NWPAEALAPN
3110 3120 3130 3140 3150
LQAASPIPKS RVAAEYKKLT EKYMDLALKQ VFAEFPGSVH DPPDSREEEE
3160 3170 3180 3190 3200
GEGEAGQGAE EWQEEEWEER EEPRPPRRLG ERGRREREVV EAVAPAPRDY
3210 3220 3230 3240 3250
SRPRVPPRRE PSGGPRSPAR DRDTLGGGAG SREKVSEGRG RPRVQLSRSP
3260 3270 3280 3290 3300
KPRPAASQVQ GPREEVGFSP GRARRGGSTA HAPPETDTAD YIEPPKSGVG
3310 3320 3330 3340 3350
AGSGPPEKKQ QGAAEAEAPA PREGEGAAQE APGEGTPEPA SIGKTSKGKK
3360 3370 3380 3390 3400
KPLFTKLPPS SVDLKDLFKK PGLGIYGGLT SPRFSDESES AGEGKGESES
3410 3420 3430
EPESPNTTAL PASGETSRSL SRRQVLLSKT QTRLHF
Length:3,436
Mass (Da):367,372
Last modified:November 1, 1996 - v1
Checksum:i663BBC19D77EF818
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U20824 Genomic DNA. Translation: AAC13852.1.
PIRiS55659.
RefSeqiNP_042661.1. NC_001650.1.

Genome annotation databases

GeneIDi1461045.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U20824 Genomic DNA. Translation: AAC13852.1 .
PIRi S55659.
RefSeqi NP_042661.1. NC_001650.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 1461045.

Family and domain databases

InterProi IPR006928. Pept_C76_UL36-USP.
[Graphical view ]
Pfami PF04843. Herpes_teg_N. 1 hit.
[Graphical view ]
PROSITEi PS51521. HTUSP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiDEN_EHV2
AccessioniPrimary (citable) accession number: Q66666
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 8, 2011
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3