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Q66666

- DEN_EHV2

UniProt

Q66666 - DEN_EHV2

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Protein
Deneddylase
Gene
64
Organism
Equine herpesvirus 2 (strain 86/87) (EHV-2)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Deneddylase that deregulates the host cell cycle S phase to create a favorable environment allowing efficient viral genome replication. Interacts with and deneddylates host cullins including CUL1 and CUL4A, thereby reducing their E3 ubiquitin ligase activity. Inhibition of cullins leads to the stabilization of cullin-RING ligase substrates such as host CDN1A/p21, CDKN1B/p27kip and CDC25A, preventing S phase progression. Additionally, acts as a deubiquitinase and cleaves both 'Lys-48' and 'Lys-63'-linked ubiquitin chains By similarity.

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei47 – 471 By similarity
Active sitei179 – 1791 By similarity

GO - Molecular functioni

  1. cysteine-type peptidase activity Source: UniProtKB-KW

GO - Biological processi

  1. modulation by virus of host protein ubiquitination Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Host-virus interaction, Modulation of host ubiquitin pathway by viral deubiquitinase, Modulation of host ubiquitin pathway by virus, Ubl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Deneddylase (EC:3.4.19.12, EC:3.4.22.-)
Alternative name(s):
Ubiquitin thioesterase
Gene namesi
Name:64
OrganismiEquine herpesvirus 2 (strain 86/87) (EHV-2)
Taxonomic identifieri82831 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeGammaherpesvirinaePercavirus
Virus hostiEquus caballus (Horse) [TaxID: 9796]
ProteomesiUP000007083: Genome

Subcellular locationi

Virion tegument. Host cytoplasm. Host nucleus By similarity
Note: Tightly associated with the capsid By similarity.

GO - Cellular componenti

  1. host cell cytoplasm Source: UniProtKB-SubCell
  2. host cell nucleus Source: UniProtKB-SubCell
  3. viral tegument Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Host nucleus, Virion, Virion tegument

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 34363436Deneddylase
PRO_0000406058Add
BLAST

Interactioni

Subunit structurei

Interacts with host CUL1 and CUL4A; these interactions inhibit the E3 ligase activity of cullins By similarity.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini27 – 241215Peptidase C76
Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili1761 – 179535 Reviewed prediction
Add
BLAST
Coiled coili2038 – 207538 Reviewed prediction
Add
BLAST
Coiled coili2177 – 224670 Reviewed prediction
Add
BLAST
Coiled coili2296 – 232631 Reviewed prediction
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi710 – 1249540Gly-rich
Add
BLAST
Compositional biasi1369 – 13724Poly-Ser
Compositional biasi2577 – 2793217Gly-rich
Add
BLAST
Compositional biasi3147 – 319145Glu-rich
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Coiled coil, Repeat

Family and domain databases

InterProiIPR006928. Pept_C76_UL36-USP.
[Graphical view]
PfamiPF04843. Herpes_teg_N. 1 hit.
[Graphical view]
PROSITEiPS51521. HTUSP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q66666-1 [UniParc]FASTAAdd to Basket

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MEGIKIDELD LKTQAIFLHY QKFTLEGLAS NHQGVACKFG RYAGMQCLSN     50
CTAYLLYTYY NGGVPLKDRT ELDHVLNVGS KIDFVLRQGG SIAPDQYVQI 100
AEIPGTVITS QVNCHIYQSN EIFGLLGVDA PVIHDDVRSL KHVLTRNYNR 150
VTQYMICICN AMALAVVIKD KRYYLFNPHC VPGIANSVAH VISTPDADDL 200
VSYVGPTGVE YTGAFLYFVP AEVDIGNHAA FIANHYKVLR FNRPNAIQVD 250
VSEADGGGAC IRELPKDSSD GAPADGNSAP PLESKRTRSA ESAKEVGQKL 300
VKRRQKKGFA PYTKSKPPNG RDEAAADEVA RVLPNTGATP ERTVSPGVAP 350
MSEDRPASED FYPDSEHPDS DFSDTDPDLA QSQLDESLLA RDQSLEPNAD 400
VEFGAEGAPR TAPETPVVGD GNSSFDSFHL PTTRDPGERC GRVTAADEHA 450
VGGASLGVES LQPPTTLAPD ERSRGRSGDG RGGPSAADDR VVGVVNSSDN 500
SFQTPTTPAP DESRRLSRLE GEGGDAGTAS EDVAVPGGDS RRAGEKVTHP 550
HTGPSARGSR SPEGVPGAED AGRGASPGHR VPVSARSSQA QQAVSGPNSQ 600
AEGDEGLPPR DTDGRGKAAR RSRPGGDGAP AFNLWHGDVN SHKESPPPRP 650
SEKTGEDPSM VKTAKGDMSP LYGDPSTGTL MHKCSDRPTP ESIHACTREL 700
DENMVLDEDG GSGCGDGRGD VRSGGESAPS DRNSLSESAS LGARPPPERR 750
LPETREDWEG EGDAMMAIEA GPEGDSAKRP VYGKVSADGG SSFPDAGASA 800
RKTGPRAAKK KGGEKKRKGR GVEGGSIPAS SPGSEVGCEG GARSPTAHPA 850
QLAQREEGVS DGGDGYSSAD SLVTGYEGNY RERSHPVTSL PTEDEDMAFE 900
EEGEREGGAA AEETSDARGA QGTGDFNSLK QPKTPLSCLP LDQKPGSRVR 950
GKKGRNRGGA AGEERVVAGG KLDAGYTDPG DVTVFPASSF GPCETVSLGA 1000
SGSAHARQEG GGNEELPSDE SIGSECLSRS ESLKHSGSYR PSERARGGRG 1050
SVEDGEGSYD GNFPGEGPRR PGTTDLSEGY LVPRRQFLSR DRRRGGEEGG 1100
GEDDGMSAED GLPPDEKWLP MTAELSGDSA VRDNHFSDGD ERRGARGNGG 1150
EGWGDDTLRT DSWRNLKTTD LGEDSVVSGS RYPSMEQQSV GEGGRDGLPA 1200
DGKREPKAAD RGSGSTAPGS RVPSAGREPG GQCEGEDHVA MDEGAPGPGS 1250
PLVKKKQDND TLFPHRGDHR APEPANPVSL LVLANPPSAP VSRAAEDMLT 1300
DMASLKRKRS RAGTASDSED DAGPNDYHAH QSKSANWGDA LDDDIWIDDV 1350
PEPARSPESP DLGSERLFSS SSEESLHTAD EGGDDGAGEG GAGDEEGSGL 1400
EEDDEFEGEG ARGATAQGQG VDFSEVDTFI GNMRQFSHRE SLPLIVDASI 1450
SRGFREANAL HTIDKLITNI IIESGVVTGP AHPSKAKNLL RFIVLWGRKL 1500
GIPTRELEIF METELEISRL CSAIDDGAFP EGLFLSHVLT KVNRCLPAIY 1550
TQTRGIVKKI ASILTAETQK VEGRETVADP RGYAGILTEA FDKDAYIICT 1600
KGESVEIVKK IAALREAVAA RNAEIARESN SFEALMGALR AYGPVPEGVG 1650
PMESQPGVKD RAFAEAVGEV MGDLTERVRN VTSEFTDSLA AGHMDTTYLP 1700
DLETLIQNID STIRLLGFAA RELRMEKKPL MAAAQQLTYM GGELAAIANA 1750
DWAHRPAEPV TQVKEINDLR SRLAQLQKED ENAQATERIL ADVEGMLDDI 1800
TSQRGEGGAV SRTLTVPMLE NYLKNAGALI GESHNEKYLR LKERLRELAS 1850
SEDFLVNLIN TTTLFSVTQT LAKIEQILGG SPHLATGEKV RQAFEGAGEQ 1900
IIADAMEAVA ARDPARLDVA ATGSLPRFLR HSPAPGAEDT GAALFALSEA 1950
VKLEKGSKGR IRWRGVEGKL SDAHSLVARS AAKGAQKRKL FALIQGLRKD 2000
YAAQEQREIM EDWKAFVTEA PINSMEDVND ILRAAPNEEA REFAAKKLEE 2050
RVREMREAEA KLEKEAEEML TQAVKRRGLQ CWGRIQNAFD NMAFGGITGE 2100
DWAAVAAEFQ REGSTLSSTL PGQLSKLTDK VEAEVEALLL NKVVSMLPNG 2150
PAFKPPAFDW LTPYRAHMNF YLKSFPLPKL NRQAEAVEAK MSQIEQAIEG 2200
ADVYEAVAGT PLEAPVARAL RLLRAARDEA AGLKGQIDEG ERAYVQGVER 2250
AGEGGEPPAK PKAEIPKKLL TYEQTLSLAN LPEDFQKNVL QNETLMLNQL 2300
REYLGRVTEN INSLESRAKT SRGEANARLA AVIEENLPQA NVSISSRRLD 2350
KSDPVGFLEG IVRDKQIVES DPYSATRESL VWLHRTFKAL LPLCPASLKR 2400
RMELLGEEIL REKGRGRGAP RVGVRAHETD DVGVLTEAIE TLEARRVTGG 2450
KAAVEGWVKK RDAYRNMVED LALRSEVEKR LGPLVEKSRE ALDAPDLAVL 2500
QEEAAALLLE AKTGGLDKSA PETHERVLEL QMYLRFKLDF LKHYLDSQRP 2550
VFEAAPLSRA LYWSNSQGER ESRENSGEGE GVEGGERRGE GGGEGSPSGA 2600
PLRLAPRLSI YRSLARQATK FPGPVAGDQA LRGPGPRDPG AQRAGGPPST 2650
CSPSSPTSWS CCSSSPRRTR GWGPAGGPVR DSGQEAGGGA AGHAGQPVGG 2700
HSGPRAGRPG RLPRQQDRVR AGQPLLRHDP LRPRGQRGRP GAGRGAEKPR 2750
GRGPARDPPP VGAAAAGHPA RAGGRLPARP LPEGGGGGAQ DRGPLPAALT 2800
PTLFLEDMYF PRSRPEVDHL KALPPPRAFL FRPGRWPAAD VTQALWGSDG 2850
FAPLFPPKED GRARAGFLLW ALLTVDHVVL GQLWSTLPPL DMGLKSPLHL 2900
LYALAAAEYP NTVPDAERES GGEPGTPYPY GPPTGSYYTL GPTEAGGAEK 2950
VPVTGFEVAV GCLLRGVSAH IFIDSERPWA PKTNSGGEID LVAPVLDCTG 3000
AVDPFKSLKA VPRKTLSPSA ELFDGLAGRE EVEVFGRQAA WLGERKRGGR 3050
KRDRFIVVLD PNNYLKGSYL GNARPPGDAP PVELAIEENK NWPAEALAPN 3100
LQAASPIPKS RVAAEYKKLT EKYMDLALKQ VFAEFPGSVH DPPDSREEEE 3150
GEGEAGQGAE EWQEEEWEER EEPRPPRRLG ERGRREREVV EAVAPAPRDY 3200
SRPRVPPRRE PSGGPRSPAR DRDTLGGGAG SREKVSEGRG RPRVQLSRSP 3250
KPRPAASQVQ GPREEVGFSP GRARRGGSTA HAPPETDTAD YIEPPKSGVG 3300
AGSGPPEKKQ QGAAEAEAPA PREGEGAAQE APGEGTPEPA SIGKTSKGKK 3350
KPLFTKLPPS SVDLKDLFKK PGLGIYGGLT SPRFSDESES AGEGKGESES 3400
EPESPNTTAL PASGETSRSL SRRQVLLSKT QTRLHF 3436
Length:3,436
Mass (Da):367,372
Last modified:November 1, 1996 - v1
Checksum:i663BBC19D77EF818
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U20824 Genomic DNA. Translation: AAC13852.1.
PIRiS55659.
RefSeqiNP_042661.1. NC_001650.1.

Genome annotation databases

GeneIDi1461045.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U20824 Genomic DNA. Translation: AAC13852.1 .
PIRi S55659.
RefSeqi NP_042661.1. NC_001650.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 1461045.

Family and domain databases

InterProi IPR006928. Pept_C76_UL36-USP.
[Graphical view ]
Pfami PF04843. Herpes_teg_N. 1 hit.
[Graphical view ]
PROSITEi PS51521. HTUSP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiDEN_EHV2
AccessioniPrimary (citable) accession number: Q66666
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 8, 2011
Last sequence update: November 1, 1996
Last modified: May 14, 2014
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3