Q66663 (RIR1_EHV2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonucleoside-diphosphate reductase large subunit
EC=1.17.4.1

Alternative name(s):
Ribonucleotide reductase large subunit

Gene names

Name:

61

Organism

Equine herpesvirus 2 (strain 86/87) (EHV-2) [Reference proteome]

Taxonomic identifier

82831 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Herpesvirales › Herpesviridae › Gammaherpesvirinae › Percavirus › Equid herpesvirus 2

Virus host

Equus caballus (Horse) [TaxID: 9796]

Protein attributes

Sequence length	799 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.
Catalytic activity	2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.
Pathway	Genetic information processing; DNA replication.
Sequence similarities	Belongs to the ribonucleoside diphosphate reductase large chain family.

Ontologies

Keywords
Biological process	DNA replication
Developmental stage	Early protein
Ligand	ATP-binding Nucleotide-binding
Molecular function	Oxidoreductase
PTM	Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA replication Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 799	799	Ribonucleoside-diphosphate reductase large subunit		PRO_0000405987
Regions
	Region	207 – 208	2	Substrate binding By similarity
	Region	408 – 412	5	Substrate binding By similarity
	Region	612 – 616	5	Substrate binding By similarity
Sites
	Active site	408	1	Proton acceptor By similarity
	Active site	410	1	Cysteine radical intermediate By similarity
	Active site	412	1	Proton acceptor By similarity
	Binding site	192	1	Substrate By similarity
	Binding site	238	1	Substrate; via amide nitrogen By similarity
	Site	208	1	Important for hydrogen atom transfer By similarity
	Site	424	1	Important for hydrogen atom transfer By similarity
	Site	743	1	Important for electron transfer By similarity
	Site	744	1	Important for electron transfer By similarity
	Site	795	1	Interacts with thioredoxin/glutaredoxin By similarity
	Site	798	1	Interacts with thioredoxin/glutaredoxin By similarity
Amino acid modifications
	Disulfide bond	208 ↔ 424		Redox-active By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q66663 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: A88D2540357934F0	FASTA	799	88,581
	10 20 30 40 50 60 MAAAHLGMPV PVYMAEREED LALRKLIDTL KISAGWDLEA NRMAGRLYHF VMGRRAPVDT 70 80 90 100 110 120 RAYVTTFGDK LEKGVHRFLW DNARDIDDLC KDFQNGPEYE RLISRGMLSA KRMYDTYVLR 130 140 150 160 170 180 TEEGGGEGAV YESALQMYAR MAAFFTCQCF AYPSLRKTVS EVEGGSGRME SGLDFFAYFF 190 200 210 220 230 240 KILASQLVSC ATPVMRSAGL RQSYLASCFI MNPDMSTEDK TLSAVFRDLS PLLCSKSGVG 250 260 270 280 290 300 MNLTNFSSGG KNVQSCLRLI NSQVEFCNDK NLRPVSVAAY MELWHEQIEE FLAAKLPENP 310 320 330 340 350 360 ERCQSIFQGV CVPGLFFRLY EQNPDSQWHL FSPKVGGHLA GLYGDKFDEE YARLVRHGLY 370 380 390 400 410 420 SSSLPAKSLM FALISAIIKT GSPYILSKDA INRHHWFETQ GNAISYANLC AEVVQQPHEF 430 440 450 460 470 480 TSTCNLANVC LPACLRARGE GEGAGAAGEG EGADRLSPLT PDLEFCFDTL RAAVRAAVYM 490 500 510 520 530 540 INASILGGVC PTPGVRVMQA ERSAGIGVQG LADVFAKLGR GYMDAESALL DARIFEVMYY 550 560 570 580 590 600 QAVRASNQLV TVGGAPPHAN WKNSKLSRGE FHWESWGVQY DSLFIERQLW EELRESVTKH 610 620 630 640 650 660 GTFNSQFIAL MPTAGTSQLT GLSESFYPFY ANVSSKVSNK EEVMKPNITF LERVSPSDVP 670 680 690 700 710 720 LLKYHGGDVS KLPPPLAAKY RNFLTAFDYS PEDQIRRAAA RSPFVDQSQS FSFFLKEANV 730 740 750 760 770 780 KSASYVKNLI LLGHGLGLKT IMYYCRIQKQ TSLTALECLQ CTESPDSGDG VGGYKGGDEE 790 PRSPEHAQCE SPDRCLSCQ

References

[1]	"The DNA sequence of equine herpesvirus 2." Telford E.A.R., Watson M.S., Aird H.C., Perry J., Davison A.J. J. Mol. Biol. 249:520-528(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	U20824 Genomic DNA. Translation: AAC13849.1.
PIR	S55656.
RefSeq	NP_042658.1. NC_001650.1.
3D structure databases
ProteinModelPortal	Q66663.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1461053.
Phylogenomic databases
ProtClustDB	CLSP2509697.
Enzyme and pathway databases
UniPathway	UPA00326.
Family and domain databases
InterPro	IPR013346. NrdE_NrdA. IPR000788. RNR_lg_C. IPR013509. RNR_lsu_N. [Graphical view]
Pfam	PF02867. Ribonuc_red_lgC. 1 hit. PF00317. Ribonuc_red_lgN. 1 hit. [Graphical view]
PRINTS	PR01183. RIBORDTASEM1.
TIGRFAMs	TIGR02506. NrdE_NrdA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RIR1_EHV2

Accession

Primary (citable) accession number: Q66663

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 8, 2011
Last sequence update:	November 1, 1996
Last modified:	May 29, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program