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Q66663 (RIR1_EHV2) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribonucleoside-diphosphate reductase large subunit

EC=1.17.4.1
Alternative name(s):
Ribonucleotide reductase large subunit
Gene names
Name:61
OrganismEquine herpesvirus 2 (strain 86/87) (EHV-2) [Reference proteome]
Taxonomic identifier82831 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeGammaherpesvirinaePercavirus
Virus hostEquus caballus (Horse) [TaxID: 9796]

Protein attributes

Sequence length799 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.

Catalytic activity

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Pathway

Genetic information processing; DNA replication.

Sequence similarities

Belongs to the ribonucleoside diphosphate reductase large chain family.

Ontologies

Keywords
   Biological processDNA replication
   Developmental stageEarly protein
   LigandATP-binding
Nucleotide-binding
   Molecular functionOxidoreductase
   PTMDisulfide bond
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processDNA replication

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 799799Ribonucleoside-diphosphate reductase large subunit
PRO_0000405987

Regions

Region207 – 2082Substrate binding By similarity
Region408 – 4125Substrate binding By similarity
Region612 – 6165Substrate binding By similarity

Sites

Active site4081Proton acceptor By similarity
Active site4101Cysteine radical intermediate By similarity
Active site4121Proton acceptor By similarity
Binding site1921Substrate By similarity
Binding site2381Substrate; via amide nitrogen By similarity
Site2081Important for hydrogen atom transfer By similarity
Site4241Important for hydrogen atom transfer By similarity
Site7431Important for electron transfer By similarity
Site7441Important for electron transfer By similarity
Site7951Interacts with thioredoxin/glutaredoxin By similarity
Site7981Interacts with thioredoxin/glutaredoxin By similarity

Amino acid modifications

Disulfide bond208 ↔ 424Redox-active By similarity

Sequences

Sequence LengthMass (Da)Tools
Q66663 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: A88D2540357934F0

FASTA79988,581
        10         20         30         40         50         60 
MAAAHLGMPV PVYMAEREED LALRKLIDTL KISAGWDLEA NRMAGRLYHF VMGRRAPVDT 

        70         80         90        100        110        120 
RAYVTTFGDK LEKGVHRFLW DNARDIDDLC KDFQNGPEYE RLISRGMLSA KRMYDTYVLR 

       130        140        150        160        170        180 
TEEGGGEGAV YESALQMYAR MAAFFTCQCF AYPSLRKTVS EVEGGSGRME SGLDFFAYFF 

       190        200        210        220        230        240 
KILASQLVSC ATPVMRSAGL RQSYLASCFI MNPDMSTEDK TLSAVFRDLS PLLCSKSGVG 

       250        260        270        280        290        300 
MNLTNFSSGG KNVQSCLRLI NSQVEFCNDK NLRPVSVAAY MELWHEQIEE FLAAKLPENP 

       310        320        330        340        350        360 
ERCQSIFQGV CVPGLFFRLY EQNPDSQWHL FSPKVGGHLA GLYGDKFDEE YARLVRHGLY 

       370        380        390        400        410        420 
SSSLPAKSLM FALISAIIKT GSPYILSKDA INRHHWFETQ GNAISYANLC AEVVQQPHEF 

       430        440        450        460        470        480 
TSTCNLANVC LPACLRARGE GEGAGAAGEG EGADRLSPLT PDLEFCFDTL RAAVRAAVYM 

       490        500        510        520        530        540 
INASILGGVC PTPGVRVMQA ERSAGIGVQG LADVFAKLGR GYMDAESALL DARIFEVMYY 

       550        560        570        580        590        600 
QAVRASNQLV TVGGAPPHAN WKNSKLSRGE FHWESWGVQY DSLFIERQLW EELRESVTKH 

       610        620        630        640        650        660 
GTFNSQFIAL MPTAGTSQLT GLSESFYPFY ANVSSKVSNK EEVMKPNITF LERVSPSDVP 

       670        680        690        700        710        720 
LLKYHGGDVS KLPPPLAAKY RNFLTAFDYS PEDQIRRAAA RSPFVDQSQS FSFFLKEANV 

       730        740        750        760        770        780 
KSASYVKNLI LLGHGLGLKT IMYYCRIQKQ TSLTALECLQ CTESPDSGDG VGGYKGGDEE 

       790 
PRSPEHAQCE SPDRCLSCQ 

« Hide

References

[1]"The DNA sequence of equine herpesvirus 2."
Telford E.A.R., Watson M.S., Aird H.C., Perry J., Davison A.J.
J. Mol. Biol. 249:520-528(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U20824 Genomic DNA. Translation: AAC13849.1.
PIRS55656.
RefSeqNP_042658.1. NC_001650.1.

3D structure databases

ProteinModelPortalQ66663.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1461053.

Phylogenomic databases

ProtClustDBCLSP2509697.

Enzyme and pathway databases

UniPathwayUPA00326.

Family and domain databases

InterProIPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
[Graphical view]
PfamPF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view]
PRINTSPR01183. RIBORDTASEM1.
TIGRFAMsTIGR02506. NrdE_NrdA. 1 hit.
ProtoNetSearch...

Entry information

Entry nameRIR1_EHV2
AccessionPrimary (citable) accession number: Q66663
Entry history
Integrated into UniProtKB/Swiss-Prot: March 8, 2011
Last sequence update: November 1, 1996
Last modified: October 16, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways