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Protein

Ribonucleoside-diphosphate reductase large subunit

Gene

RIR1

Organism
Equine herpesvirus 2 (strain 86/87) (EHV-2)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Ribonucleoside-diphosphate reductase holoenzyme provides the precursors necessary for viral DNA synthesis. Allows virus growth in non-dividing cells, as well as reactivation from latency in infected hosts. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.UniRule annotation

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.UniRule annotation

Pathwayi: DNA replication

This protein is involved in the pathway DNA replication, which is part of Genetic information processing.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway DNA replication and in Genetic information processing.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei192 – 1921SubstrateUniRule annotation
Sitei208 – 2081Important for hydrogen atom transferUniRule annotation
Binding sitei238 – 2381Substrate; via amide nitrogenUniRule annotation
Active sitei408 – 4081Proton acceptorUniRule annotation
Active sitei410 – 4101Cysteine radical intermediateUniRule annotation
Active sitei412 – 4121Proton acceptorUniRule annotation
Sitei424 – 4241Important for hydrogen atom transferUniRule annotation
Sitei743 – 7431Important for electron transferUniRule annotation
Sitei744 – 7441Important for electron transferUniRule annotation
Sitei795 – 7951Interacts with thioredoxin/glutaredoxinUniRule annotation
Sitei798 – 7981Interacts with thioredoxin/glutaredoxinUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase large subunitUniRule annotation (EC:1.17.4.1UniRule annotation)
Short name:
R1UniRule annotation
Alternative name(s):
Ribonucleotide reductase large subunitUniRule annotation
Gene namesi
Name:RIR1UniRule annotation
Ordered Locus Names:61
OrganismiEquine herpesvirus 2 (strain 86/87) (EHV-2)
Taxonomic identifieri82831 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeGammaherpesvirinaePercavirus
Virus hostiEquus caballus (Horse) [TaxID: 9796]
Proteomesi
  • UP000007083 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 799799Ribonucleoside-diphosphate reductase large subunitPRO_0000405987Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi208 ↔ 424Redox-activeUniRule annotation

Keywords - PTMi

Disulfide bond

Expressioni

Keywords - Developmental stagei

Early protein

Interactioni

Subunit structurei

Heterotetramer composed of a homodimer of the large subunit (R1) and a homodimer of the small subunit (R2). Larger multisubunit protein complex are also active, composed of (R1)n(R2)n.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ66663.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni207 – 2082Substrate bindingUniRule annotation
Regioni408 – 4125Substrate bindingUniRule annotation
Regioni612 – 6165Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the ribonucleoside diphosphate reductase large chain family.UniRule annotation

Phylogenomic databases

KOiK10807.

Family and domain databases

HAMAPiMF_04026. HSV_RIR1. 1 hit.
InterProiIPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
[Graphical view]
PfamiPF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q66663-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAAHLGMPV PVYMAEREED LALRKLIDTL KISAGWDLEA NRMAGRLYHF
60 70 80 90 100
VMGRRAPVDT RAYVTTFGDK LEKGVHRFLW DNARDIDDLC KDFQNGPEYE
110 120 130 140 150
RLISRGMLSA KRMYDTYVLR TEEGGGEGAV YESALQMYAR MAAFFTCQCF
160 170 180 190 200
AYPSLRKTVS EVEGGSGRME SGLDFFAYFF KILASQLVSC ATPVMRSAGL
210 220 230 240 250
RQSYLASCFI MNPDMSTEDK TLSAVFRDLS PLLCSKSGVG MNLTNFSSGG
260 270 280 290 300
KNVQSCLRLI NSQVEFCNDK NLRPVSVAAY MELWHEQIEE FLAAKLPENP
310 320 330 340 350
ERCQSIFQGV CVPGLFFRLY EQNPDSQWHL FSPKVGGHLA GLYGDKFDEE
360 370 380 390 400
YARLVRHGLY SSSLPAKSLM FALISAIIKT GSPYILSKDA INRHHWFETQ
410 420 430 440 450
GNAISYANLC AEVVQQPHEF TSTCNLANVC LPACLRARGE GEGAGAAGEG
460 470 480 490 500
EGADRLSPLT PDLEFCFDTL RAAVRAAVYM INASILGGVC PTPGVRVMQA
510 520 530 540 550
ERSAGIGVQG LADVFAKLGR GYMDAESALL DARIFEVMYY QAVRASNQLV
560 570 580 590 600
TVGGAPPHAN WKNSKLSRGE FHWESWGVQY DSLFIERQLW EELRESVTKH
610 620 630 640 650
GTFNSQFIAL MPTAGTSQLT GLSESFYPFY ANVSSKVSNK EEVMKPNITF
660 670 680 690 700
LERVSPSDVP LLKYHGGDVS KLPPPLAAKY RNFLTAFDYS PEDQIRRAAA
710 720 730 740 750
RSPFVDQSQS FSFFLKEANV KSASYVKNLI LLGHGLGLKT IMYYCRIQKQ
760 770 780 790
TSLTALECLQ CTESPDSGDG VGGYKGGDEE PRSPEHAQCE SPDRCLSCQ
Length:799
Mass (Da):88,581
Last modified:November 1, 1996 - v1
Checksum:iA88D2540357934F0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U20824 Genomic DNA. Translation: AAC13849.1.
PIRiS55656.
RefSeqiNP_042658.1. NC_001650.2.

Genome annotation databases

GeneIDi1461039.
KEGGivg:1461039.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U20824 Genomic DNA. Translation: AAC13849.1.
PIRiS55656.
RefSeqiNP_042658.1. NC_001650.2.

3D structure databases

ProteinModelPortaliQ66663.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1461039.
KEGGivg:1461039.

Phylogenomic databases

KOiK10807.

Enzyme and pathway databases

UniPathwayiUPA00326.

Family and domain databases

HAMAPiMF_04026. HSV_RIR1. 1 hit.
InterProiIPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
[Graphical view]
PfamiPF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRIR1_EHV2
AccessioniPrimary (citable) accession number: Q66663
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 8, 2011
Last sequence update: November 1, 1996
Last modified: June 8, 2016
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.