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Protein

DNA primase

Gene

56

Organism
Equine herpesvirus 2 (strain 86/87) (EHV-2)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Essential component of the helicase/primase complex. Unwinds the DNA at the replication forks and generates single-stranded DNA for both leading and lagging strand synthesis. The primase initiates primer synthesis and thereby produces large amount of short RNA primers on the lagging strand that the polymerase elongates using dNTPs.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei529Essential for primase activityUniRule annotation1
Sitei531Essential for primase activityUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri825 – 864CHC2-typeUniRule annotationAdd BLAST40

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHelicase, Hydrolase, Transferase
Biological processDNA replication
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
DNA primaseUniRule annotation (EC:2.7.7.-UniRule annotation)
Gene namesi
Name:56
OrganismiEquine herpesvirus 2 (strain 86/87) (EHV-2)
Taxonomic identifieri82831 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeGammaherpesvirinaePercavirus
Virus hostiEquus caballus (Horse) [TaxID: 9796]
Proteomesi
  • UP000007083 Componenti: Genome

Subcellular locationi

Q66658:
  • Host nucleus UniRule annotation
  • Note: Requires the presence of the primase associated factor to properly localize in the host cell nucleus.UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Host nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004060661 – 884DNA primaseAdd BLAST884

Interactioni

Subunit structurei

Associates with the helicase and the primase-associated factor to form the helicase-primase factor.UniRule annotation

Structurei

3D structure databases

SMRiQ66658.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi141 – 146Poly-Gly6
Compositional biasi377 – 381Poly-Gly5

Sequence similaritiesi

Belongs to the herpesviridae DNA primase family.UniRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri825 – 864CHC2-typeUniRule annotationAdd BLAST40

Keywords - Domaini

Zinc-finger

Phylogenomic databases

OrthoDBiVOG0900001H.

Family and domain databases

HAMAPiMF_04011. HSV_PRIM. 1 hit.
InterProiView protein in InterPro
IPR033685. HSV_PRIM.

Sequencei

Sequence statusi: Complete.

Q66658-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATGRSTSTE SSEKTCQQRD PLPEYRALCG TDGDAAEILT DVLTNTDSDG
60 70 80 90 100
VVFCLAHNCY SYNIGGGEAL LTLCLPAKRP WGAEKCLPVI QFRCDASRAQ
110 120 130 140 150
EFLFQGRPIP VRYIQTNLNH RAVKKFFKPI LSVLTCSDKK GGGGEGAHAD
160 170 180 190 200
LKSTIFWFRA KFVAAVRKTF KITASPFWMI STFGCTEAQF VLVSSCYFFE
210 220 230 240 250
RHECTIDTLS HLSRLFDGSR GRQLTTVNTF SDLAGMFGTS AWLGRVPEFS
260 270 280 290 300
AYVGKKLARD DLESAAVDEA VNAFRGQLML SNADLIHYIY LSFFQCLNKE
310 320 330 340 350
KFLEYSLRTN PHNIDGVPPE EPIITGFIDE GFKSKMATYY TKSSYLKNHV
360 370 380 390 400
RVGSLYLDGV EGYSPEAIEA GPPAAAGGGG GADRYWAGQS RDVQGLLSDI
410 420 430 440 450
LADHPASRLS PDLHGLLDLA ALGDSSGVAG GVKDSLFPEP LRCPVYRCQY
460 470 480 490 500
LNKTFFAVVT RDNLARAWER AVQLPTQVTG WEGMEDARLT ACVHYAELAF
510 520 530 540 550
SLGHLREQLS VSRHEYFNPR LPVFNLVLDF DLPLKKPGLS LERVYSICRS
560 570 580 590 600
VRSDVLSVLG VLGEVDEAAH PVYFFKSACP RPEWDEPYAG RPFCTCDAKL
610 620 630 640 650
GLRIITPLPR GVAIVGGAPL VALAKILNRM IKMNREDLLE ICPGLPDADG
660 670 680 690 700
PLDTGIYHRG RCVRLPHTYK VNEACGLERL LRLFVCHPGS PDKAAYIRDA
710 720 730 740 750
MTLRNLLHHS KSAYWEGNAR EGVSEEAPQK TKVVYSVTDV SENFLVCQTQ
760 770 780 790 800
QQLPRSYERP DSRIETMTGR DLVTWVTEVA WPKVFHNIKA YIPDDKTTQF
810 820 830 840 850
HFVKFIHTSH NIIQVKPQRG NNFVCISSNH RNKTQSVRIF IVLYTNKKDE
860 870 880
VTITLMSQCF AHKCNSNKPR AHFSIPLQLR GRDF
Length:884
Mass (Da):98,684
Last modified:November 1, 1996 - v1
Checksum:i76833888B1825537
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U20824 Genomic DNA. Translation: AAC13844.1.
PIRiS55651.
RefSeqiNP_042653.1. NC_001650.2.

Genome annotation databases

GeneIDi1461069.
KEGGivg:1461069.

Similar proteinsi

Entry informationi

Entry nameiPRIM_EHV2
AccessioniPrimary (citable) accession number: Q66658
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 8, 2011
Last sequence update: November 1, 1996
Last modified: March 15, 2017
This is version 60 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families