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Protein

Shutoff alkaline exonuclease

Gene

37

Organism
Equine herpesvirus 2 (strain 86/87) (EHV-2)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Plays a role in processing non linear or branched viral DNA intermediates in order to promote the production of mature packaged unit-length linear progeny viral DNA molecules. Exhibits endonuclease and exonuclease activities and accepts both double-stranded and single-stranded DNA as substrate. Exonuclease digestion of DNA is in the 5'-> 3' direction and the products are 5'-monophosphate nucleosides. Additionally, forms a recombinase with the major DNA-binding protein, which displays strand exchange activity. Also acts as a cytoplasmic RNA endonuclease that induces degradation of the majority of the cellular messenger RNAs during early lytic infection. The resulting inhibition of cellular protein synthesis serves to ensure maximal viral gene expression and evasion from host immune response. Internally cleaves host mRNAs which are then degraded by the cellular exonuclease XRN1. Bypasses therefore the regulatory steps of deadenylation and decapping normally required for XRN1 activation.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei207 – 2071Required for functionUniRule annotation
Sitei244 – 2441Required for functionUniRule annotation
Sitei267 – 2671Required for functionUniRule annotation
Sitei269 – 2691Required for functionUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Exonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Decay of host mRNAs by virus, Eukaryotic host gene expression shutoff by virus, Host gene expression shutoff by virus, Host mRNA suppression by virus, Host-virus interaction

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Shutoff alkaline exonucleaseUniRule annotation (EC:3.1.-.-UniRule annotation)
Short name:
SOXUniRule annotation
Gene namesi
Name:37
OrganismiEquine herpesvirus 2 (strain 86/87) (EHV-2)
Taxonomic identifieri82831 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeGammaherpesvirinaePercavirus
Virus hostiEquus caballus (Horse) [TaxID: 9796]
Proteomesi
  • UP000007083 Componenti: Genome

Subcellular locationi

  • Host nucleus UniRule annotation
  • Host cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Host nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 509509Shutoff alkaline exonucleasePRO_0000405985Add
BLAST

Expressioni

Keywords - Developmental stagei

Early protein

Interactioni

Subunit structurei

Forms a complex with the DNA polymerase, the DNA polymerase processivity factor, and the major DNA binding protein.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ66641.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the herpesviridae alkaline nuclease family.UniRule annotation

Family and domain databases

Gene3Di3.90.320.10. 1 hit.
HAMAPiMF_04009. AN_HPV.
InterProiIPR011604. Exonuc_phg/RecB_C.
IPR001616. Herpes_alk_exo.
IPR011335. Restrct_endonuc-II-like.
[Graphical view]
PfamiPF01771. Herpes_alk_exo. 1 hit.
[Graphical view]
PRINTSiPR00924. ALKEXNUCLASE.
SUPFAMiSSF52980. SSF52980. 1 hit.

Sequencei

Sequence statusi: Complete.

Q66641-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDFFSRVPLY DELSGLSQPE TKCKLEEFTF SNFLKARRVR EFLRAHAVAK
60 70 80 90 100
LPAMRYVYVY YLFSRIGDYI GDDTVLAIFG EFMGVSDAGK ARGEGDGESD
110 120 130 140 150
VHGEGEEEGD GGGEAEPGVG PDIPEVYRVC ECLPVRTKCR ISLAIEAITR
160 170 180 190 200
GQYENNLWEI FRDGIISSSK FYHAVRQQNS SKKLFQPWPI VNNYYPLSPL
210 220 230 240 250
AFGLRCEDAV KTLLAEFVCG RKEVMCDVGF LQSPKDGIFG VSLDMCANVS
260 270 280 290 300
VGRDNLLEFR ADAEIYEIKC RFKYNYSKIE CDPLYQKYVS LYNSPSKTTL
310 320 330 340 350
IRFLSGINRP AVEYVPPGKL PTKNDFLLTS DRDWDLSPKR KRNLTPAHKS
360 370 380 390 400
LYDCLRANEH ASSQVLILSD PSETEGKIDI KARFDVDVFI NPEHSYFYQI
410 420 430 440 450
LLQYKVVKNY IQYHSSPGLG SLKTFIVSGF FRKRNHSDPL ECSIGGRGTL
460 470 480 490 500
DSACEIPVLL ILTPVYIPHC VVTESLKKAS QYWNQSAEEE FSHPPWVSSS

LFADGDMTP
Length:509
Mass (Da):57,577
Last modified:November 1, 1996 - v1
Checksum:iF73DF3676DDE00F6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U20824 Genomic DNA. Translation: AAC13825.1.
PIRiS55632.
RefSeqiNP_042634.1. NC_001650.2.

Genome annotation databases

GeneIDi1461042.
KEGGivg:1461042.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U20824 Genomic DNA. Translation: AAC13825.1.
PIRiS55632.
RefSeqiNP_042634.1. NC_001650.2.

3D structure databases

ProteinModelPortaliQ66641.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1461042.
KEGGivg:1461042.

Family and domain databases

Gene3Di3.90.320.10. 1 hit.
HAMAPiMF_04009. AN_HPV.
InterProiIPR011604. Exonuc_phg/RecB_C.
IPR001616. Herpes_alk_exo.
IPR011335. Restrct_endonuc-II-like.
[Graphical view]
PfamiPF01771. Herpes_alk_exo. 1 hit.
[Graphical view]
PRINTSiPR00924. ALKEXNUCLASE.
SUPFAMiSSF52980. SSF52980. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiAN_EHV2
AccessioniPrimary (citable) accession number: Q66641
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 8, 2011
Last sequence update: November 1, 1996
Last modified: April 13, 2016
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.