Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Thymidine kinase

Gene

TK

Organism
Equine herpesvirus 2 (strain 86/87) (EHV-2)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of the gamma-phospho group of ATP to thymidine to generate dTMP in the salvage pathway of pyrimidine synthesis. The dTMP serves as a substrate for DNA polymerase during viral DNA replication. Allows the virus to be reactivated and to grow in non-proliferative cells lacking a high concentration of phosphorylated nucleic acid precursors.UniRule annotation

Catalytic activityi

ATP + thymidine = ADP + thymidine 5'-phosphate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei327 – 3271Proton acceptorUniRule annotation
Binding sitei344 – 3441SubstrateUniRule annotation
Binding sitei365 – 3651SubstrateUniRule annotation
Binding sitei461 – 4611SubstrateUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi301 – 3088ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

DNA synthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidine kinaseUniRule annotation (EC:2.7.1.21UniRule annotation)
Gene namesi
Name:TKUniRule annotation
Ordered Locus Names:21
OrganismiEquine herpesvirus 2 (strain 86/87) (EHV-2)
Taxonomic identifieri82831 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeGammaherpesvirinaePercavirus
Virus hostiEquus caballus (Horse) [TaxID: 9796]
Proteomesi
  • UP000007083 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 613613Thymidine kinasePRO_0000405986Add
BLAST

Expressioni

Keywords - Developmental stagei

Early protein

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ66624.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi9 – 157Poly-Ser

Sequence similaritiesi

Belongs to the herpesviridae thymidine kinase family.UniRule annotation

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_04029. HSV_KITH. 1 hit.
InterProiIPR001889. Herpes_TK.
IPR013672. Herpes_TK_C.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00693. Herpes_TK. 1 hit.
PF08465. Herpes_TK_C. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

Q66624-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEGGAGFSS SSTSSEEAVP WSTQQPMGWE ELESLGDGGG STSADEEFQW
60 70 80 90 100
EAMFVKSRAG SPTAEDKSRT FTLPRGRPKN EPRPERGKGK TPKKPKKPDQ
110 120 130 140 150
GATLLVGEEP RPRLGSRTRS KSRSRDKHQL PDDIYDVPNP PMLAPVDSYG
160 170 180 190 200
NPVEQVSSSE SDFEDIANIR PILRRQQPVT VKHRREPSPE PLGHPTFVHR
210 220 230 240 250
YDKPSYDEEV CQKKDKGGRT KSKNWLRQPG VKSKLTSMKD LSGSFKSLMH
260 270 280 290 300
IRSDGEKHKQ QQRPGGSGAP GGATPRDVFN TFLGSGTCPS FKNAFFLYLE
310 320 330 340 350
GSMGVGKTTL IRHMREINGD NVISFVEPMF YWREVYSDCV KLIYSACKPF
360 370 380 390 400
NLGKMSTSNK VLSAQMKFMT PMKCLQTSVR RYVKANEPLQ EKTAMDNWLL
410 420 430 440 450
FDRHPLSATL VFPYLSLKNG YLAFEHFLAL AANFTAHEGD IIALLCMGEE
460 470 480 490 500
DNLKMVKLRN RKGESGVTSA HLKDLGQAFH ACYCTWLLLK YLSPEDMVSV
510 520 530 540 550
CCCDVTLNDI CIMRSMSSSK VTMAKSLFNK SMFPTLMDVI QPFRSNCTII
560 570 580 590 600
EICLTLFMEL KKVEFIVVNA SEFIGDIPGV WTSIYTQSLR TQAIKTQSID
610
WSGLRAFSLT YNS
Length:613
Mass (Da):68,665
Last modified:November 11, 2015 - v2
Checksum:i406E210129510171
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U20824 Genomic DNA. Translation: AAC13808.2.
PIRiS55615.
RefSeqiNP_042617.2. NC_001650.2.

Genome annotation databases

GeneIDi1461018.
KEGGivg:1461018.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U20824 Genomic DNA. Translation: AAC13808.2.
PIRiS55615.
RefSeqiNP_042617.2. NC_001650.2.

3D structure databases

ProteinModelPortaliQ66624.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1461018.
KEGGivg:1461018.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_04029. HSV_KITH. 1 hit.
InterProiIPR001889. Herpes_TK.
IPR013672. Herpes_TK_C.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00693. Herpes_TK. 1 hit.
PF08465. Herpes_TK_C. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiKITH_EHV2
AccessioniPrimary (citable) accession number: Q66624
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 8, 2011
Last sequence update: November 11, 2015
Last modified: September 7, 2016
This is version 58 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.