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Protein

Major DNA-binding protein

Gene

DBP

Organism
Equine herpesvirus 2 (strain 86/87) (EHV-2)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Plays several crucial roles in viral infection. Participates in the opening of the viral DNA origin to initiate replication by interacting with the origin-binding protein. May disrupt loops, hairpins and other secondary structures present on ssDNA to reduce and eliminate pausing of viral DNA polymerase at specific sites during elongation. Promotes viral DNA recombination by performing strand-transfer, characterized by the ability to transfer a DNA strand from a linear duplex to a complementary single-stranded DNA circle. Can also catalyze the renaturation of complementary single strands. Additionally, reorganizes the host cell nucleus, leading to the formation of prereplicative sites and replication compartments. This process is driven by the protein which can form double-helical filaments in the absence of DNA.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding
Biological processDNA replication

Names & Taxonomyi

Protein namesi
Recommended name:
Major DNA-binding proteinUniRule annotation
Gene namesi
Name:DBPUniRule annotation
Synonyms:6
OrganismiEquine herpesvirus 2 (strain 86/87) (EHV-2)
Taxonomic identifieri82831 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeGammaherpesvirinaePercavirus
Virus hostiEquus caballus (Horse) [TaxID: 9796]
Proteomesi
  • UP000007083 Componenti: Genome

Subcellular locationi

  • Host nucleus UniRule annotation
  • Note: In the absence of DNA replication, found in the nuclear framework-associated structures (prereplicative sites). As viral DNA replication proceeds, it migrates to globular intranuclear structures (replication compartments).UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Host nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004061681 – 1145Major DNA-binding proteinAdd BLAST1145

Interactioni

Subunit structurei

Homooligomers. Forms double-helical filaments necessary for the formation of replication compartments within the host nucleus. Interacts with the origin-binding protein. Interacts with the helicase primase complex; this interaction stimulates primer synthesis activity of the helicase-primase complex. Interacts with the DNA polymerase. Interacts with the alkaline exonuclease; this interaction increases its nuclease processivity.UniRule annotation

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1115 – 1145Required for nuclear localizationUniRule annotationAdd BLAST31

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi364 – 367Poly-Gly4

Sequence similaritiesi

Belongs to the herpesviridae major DNA-binding protein family.UniRule annotation

Phylogenomic databases

OrthoDBiVOG0900000X.

Family and domain databases

HAMAPiMF_04007. HSV_DNBI. 1 hit.
InterProiView protein in InterPro
IPR035989. DBP_sf.
IPR000635. Viral_ssDNA-bd.
PfamiView protein in Pfam
PF00747. Viral_DNA_bp. 1 hit.
SUPFAMiSSF118208. SSF118208. 1 hit.

Sequencei

Sequence statusi: Complete.

Q66611-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNSNRAAAGP VEENVGTQAS VGPCGFVYLY PGDTFPVEEA SLLGNLHAGG
60 70 80 90 100
EVFSLPLLSG LTVEADFHVN VKAVHKKLDP ATVSVKASAY HREVIVFANA
110 120 130 140 150
ACFKPIFAGP GLEGLCAASR QLFGYAEFEE RAGGAARPFE LADLGHLLPG
160 170 180 190 200
AESHIAGVVV TESFKERLYR GQLVVVESQI QSVRVGECDA FKVPLYDGEL
210 220 230 240 250
FAKSPCRENL RYFYHAGVSR YLFEAHYTSL AQALRVRDVP GLIGALERQS
260 270 280 290 300
FHDQYKLPKV YECREFPATG HRGAGDCSLT IVDSVATELA VSYGLSFLEV
310 320 330 340 350
PQEGTALLSY DKWPIFEGCE TPEQRVEALT QFNAKQAVHV HSQLFSGNSV
360 370 380 390 400
LYLARVQKQA SNRGGGGENV YNSFFMGHGL ACLAEPTQKE NGLPSFPGVP
410 420 430 440 450
ASALSGSNYS LHHLAYAASF SPQMLARHCY YLQFAQHQKS SNNSGYNVPT
460 470 480 490 500
YVGTAANTPM CELCRGSCPA SCVNTLFYRL RDRFPPVVAS VRRDPYVVTG
510 520 530 540 550
VAGAYNDLDI AGNFANYRDK DEESNQSEER EKFTYWQVTQ TVLERLSEAG
560 570 580 590 600
ICEGGEDVGD AIHNIGSFLK VFKEIDGIVD GEVARFINSM VKNNVNYRES
610 620 630 640 650
IKSIHHIVQY VCNVYWQPPC PVFLNLYYRC VLAVVQDICL PTCMMYEQEN
660 670 680 690 700
PAVGVSPGEW LKMHYQTLWT NFKNSCIDKG VLTGTEYKVV HKDQFCDFFD
710 720 730 740 750
VDSAARGEFV SCKTQVRISR ALMMAPRVMK IKNRIIFSNS PGTESIQNAF
760 770 780 790 800
VRGTPKGDSC VVSGPYMRFL STYHSQLFPG AKISPLFLWH TFSKKRQLPV
810 820 830 840 850
FPNVPRESVT ELANYVEQNS RLHGETSIID VVPENFYTYA KVRLNNALFR
860 870 880 890 900
ACGQTQFYAT TIHCLTPKIQ TVPAEEYPHA LGARGVADVG EYLGAARELT
910 920 930 940 950
VPTVQCTSRD NICEVGKCRP IVTLPLVVNK YTGVTGNSQI FQCANLGYFI
960 970 980 990 1000
GRGVDKNLIP DAGSFKKQGV STSMRKRHVF MTPLSDHLLR RSVQGAAVAF
1010 1020 1030 1040 1050
EIEGVRRRVQ QILSDGDNPH VIRDVVLQLV KSLGSECRSV SEYDLEYYMG
1060 1070 1080 1090 1100
QYYIFAGDVS ERLQRLSDLG GDWSEEWALS VLGEEEDPLG GELEFEKVED
1110 1120 1130 1140
AECLGHPQQD EFALAPQAAA PQYSGSSSVA GKKRKANVIL GDLDL
Length:1,145
Mass (Da):126,491
Last modified:November 1, 1996 - v1
Checksum:iAB45BCDD3C5C472C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U20824 Genomic DNA. Translation: AAC13793.1.
PIRiS55600.
RefSeqiNP_042602.1. NC_001650.2.

Genome annotation databases

GeneIDi1461061.
KEGGivg:1461061.

Similar proteinsi

Entry informationi

Entry nameiDNBI_EHV2
AccessioniPrimary (citable) accession number: Q66611
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 5, 2011
Last sequence update: November 1, 1996
Last modified: October 25, 2017
This is version 65 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families