Q664K7 (PCKA_YERPS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoenolpyruvate carboxykinase [ATP]
Short name=PEP carboxykinase
Short name=PEPCK
EC=4.1.1.49

Alternative name(s):
Phosphoenolpyruvate carboxylase

Gene names

Name:	pckA
Ordered Locus Names:	YPTB3762

Organism

Yersinia pseudotuberculosis serotype I (strain IP32953) [Complete proteome] [HAMAP]

Taxonomic identifier

273123 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Yersinia › Yersinia pseudotuberculosis

Protein attributes

Sequence length	539 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + oxaloacetate = ADP + phosphoenolpyruvate + CO2. HAMAP-Rule MF_00453
Pathway	Carbohydrate biosynthesis; gluconeogenesis. HAMAP-Rule MF_00453
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00453.
Sequence similarities	Belongs to the phosphoenolpyruvate carboxykinase [ATP] family.

Ontologies

Keywords
Biological process	Gluconeogenesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Decarboxylase Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	gluconeogenesis Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP phosphoenolpyruvate carboxykinase (ATP) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 539	539	Phosphoenolpyruvate carboxykinase [ATP] HAMAP-Rule MF_00453		PRO_0000203860
Regions
	Nucleotide binding	247 – 254	8	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q664K7 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 5A379FB1417974DB	FASTA	539	59,321
	10 20 30 40 50 60 MSVKGITPQE LAAYGIHNVS EIVYNPSYDL LFEEETKPTL EGYERGTLTT TGAIAVDTGI 70 80 90 100 110 120 FTGRSPKDKY IVRDAITQDT VWWADQGKGK NDNKPLSQEI WSHLKGLVTE QLSGKRLFVV 130 140 150 160 170 180 DTFCGANADT RLQVRFITEV AWQAHFVKNM FIRPSDEELA RFEPDFIVMN GAKCTNPQWK 190 200 210 220 230 240 EQGLNSENFV AFNLTERMQL IGGTWYGGEM KKGMFSMMNY LLPLKGIASM HCSANVGEKG 250 260 270 280 290 300 DVAIFFGLSG TGKTTLSTDP KRKLIGDDEH GWDDDGVFNF EGGCYAKTIK LSEEAEPDIY 310 320 330 340 350 360 HAIKRDALLE NVVVLADGTV DFNDGSKTEN TRVSYPIYHI DNIVKPVSKA GHATKVIFLT 370 380 390 400 410 420 ADAFGVLPPV SRLTANQTQY HFLSGFTAKL AGTERGVTEP TPTFSACFGA AFLSLHPTQY 430 440 450 460 470 480 AEVLVKRMQA VGAQAYLVNT GWNGTGKRIS IKDTRAIIDA ILNGEIDKAE TFTLPIFDLA 490 500 510 520 530 VPMALPGVNP DILDPRDTYA DKAQWQEKAE DLAKRFATNF DKYTDTPAGA ALVSAGPKI

References

[1]

"Insights into the evolution of Yersinia pestis through whole-genome comparison with Yersinia pseudotuberculosis." Chain P.S.G., Carniel E., Larimer F.W., Lamerdin J., Stoutland P.O., Regala W.M., Georgescu A.M., Vergez L.M., Land M.L., Motin V.L., Brubaker R.R., Fowler J., Hinnebusch J., Marceau M., Medigue C., Simonet M., Chenal-Francisque V., Souza B. , Dacheux D., Elliott J.M., Derbise A., Hauser L.J., Garcia E. Proc. Natl. Acad. Sci. U.S.A. 101:13826-13831(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: IP32953.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX936398 Genomic DNA. Translation: CAH23000.1.
RefSeq	YP_072243.1. NC_006155.1.
3D structure databases
ProteinModelPortal	Q664K7.
SMR	Q664K7. Positions 5-539.
ModBase	Search...
Protein-protein interaction databases
STRING	273123.YPTB3762.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAH23000; CAH23000; YPTB3762.
GeneID	2956216.
KEGG	yps:YPTB3762.
PATRIC	18646947. VBIYerPse22266_4506.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1866.
HOGENOM	HOG000271471.
KO	K01610.
OMA	TRCAYPI.
ProtClustDB	PRK09344.
Enzyme and pathway databases
BioCyc	YPSE273123:GI1M-3880-MONOMER.
UniPathway	UPA00138.
Family and domain databases
Gene3D	3.40.449.10. 1 hit. 3.90.228.20. 2 hits.
HAMAP	MF_00453. PEPCK_ATP.
InterPro	IPR001272. PEP_carboxykinase_ATP. IPR013035. PEP_carboxykinase_C. IPR008210. PEP_carboxykinase_N. IPR015994. PEPCK_ATP_CS. [Graphical view]
Pfam	PF01293. PEPCK_ATP. 1 hit. [Graphical view]
PIRSF	PIRSF006294. PEP_crbxkin. 1 hit.
SUPFAM	SSF68923. PEP_carboxykinase_N. 1 hit.
TIGRFAMs	TIGR00224. pckA. 1 hit.
PROSITE	PS00532. PEPCK_ATP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PCKA_YERPS

Accession

Primary (citable) accession number: Q664K7

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2005
Last sequence update:	October 11, 2004
Last modified:	May 29, 2013
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program