Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q66478

- POLG_HE71B

UniProt

Q66478 - POLG_HE71B

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Genome polyprotein

Gene
N/A
Organism
Human enterovirus 71 (strain BrCr) (Ev 71)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Capsid protein VP1: Forms an icosahedral capsid of pseudo T=3 symmetry with capsid proteins VP2 and VP3. The capsid is 300 Angstroms in diameter, composed of 60 copies of each capsid protein and enclosing the viral positive strand RNA genome. Capsid protein VP1 mainly forms the vertices of the capsid. Capsid protein VP1 interacts with host cell receptor to provide virion attachment to target host cells. This attachment induces virion internalization. Tyrosine kinases are probably involved in the entry process. After binding to its receptor, the capsid undergoes conformational changes. Capsid protein VP1 N-terminus (that contains an amphipathic alpha-helix) and capsid protein VP4 are externalized. Together, they shape a pore in the host membrane through which viral genome is translocated to host cell cytoplasm. After genome has been released, the channel shrinks (By similarity).By similarity
Capsid protein VP2: Forms an icosahedral capsid of pseudo T=3 symmetry with capsid proteins VP2 and VP3. The capsid is 300 Angstroms in diameter, composed of 60 copies of each capsid protein and enclosing the viral positive strand RNA genome (By similarity).By similarity
Capsid protein VP3: Forms an icosahedral capsid of pseudo T=3 symmetry with capsid proteins VP2 and VP3. The capsid is 300 Angstroms in diameter, composed of 60 copies of each capsid protein and enclosing the viral positive strand RNA genome (By similarity).By similarity
Capsid protein VP4: Lies on the inner surface of the capsid shell. After binding to the host receptor, the capsid undergoes conformational changes. Capsid protein VP4 is released, Capsid protein VP1 N-terminus is externalized, and together, they shape a pore in the host membrane through which the viral genome is translocated into the host cell cytoplasm. After genome has been released, the channel shrinks (By similarity).By similarity
Capsid protein VP0: Component of immature procapsids, which is cleaved into capsid proteins VP4 and VP2 after maturation. Allows the capsid to remain inactive before the maturation step (By similarity).By similarity
Protein 2A: Cysteine protease that cleaves viral polyprotein and specific host proteins. It is responsible for the cleavage between the P1 and P2 regions, first cleavage occurring in the polyprotein. Cleaves also the host translation initiation factor EIF4G1, in order to shut down the capped cellular mRNA translation. Inhibits the host nucleus-cytoplasm protein and RNA trafficking by cleaving host members of the nuclear pores (By similarity).By similarity
Protein 2B: Plays an essential role in the virus replication cycle by acting as a viroporin. Creates a pore in the host reticulum endoplasmic and as a consequence releases Ca2+ in the cytoplasm of infected cell. In turn, high levels of cyctoplasmic calcium may trigger membrane trafficking and transport of viral ER-associated proteins to viroplasms, sites of viral genome replication (By similarity).By similarity
Protein 2C: Induces and associates with structural rearrangements of intracellular membranes. Displays RNA-binding, nucleotide binding and NTPase activities. May play a role in virion morphogenesis and viral RNA encapsidation by interacting with the capsid protein VP3 (By similarity).By similarity
Protein 3AB: Localizes the viral replication complex to the surface of membranous vesicles. Together with protein 3CD binds the Cis-Active RNA Element (CRE) which is involved in RNA synthesis initiation. Acts as a cofactor to stimulate the activity of 3D polymerase, maybe through a nucleid acid chaperone activity (By similarity).By similarity
Protein 3A: Localizes the viral replication complex to the surface of membranous vesicles. It inhibits host cell endoplasmic reticulum-to-Golgi apparatus transport and causes the dissassembly of the Golgi complex, possibly through GBF1 interaction. This would result in depletion of MHC, trail receptors and IFN receptors at the host cell surface (By similarity).By similarity
Viral protein genome-linked: acts as a primer for viral RNA replication and remains covalently bound to viral genomic RNA. VPg is uridylylated prior to priming replication into VPg-pUpU. The oriI viral genomic sequence may act as a template for this. The VPg-pUpU is then used as primer on the genomic RNA poly(A) by the RNA-dependent RNA polymerase to replicate the viral genome. VPg may be removed in the cytoplasm by an unknown enzyme termed "unlinkase". VPg is not cleaved off virion genomes because replicated genomic RNA are encapsidated at the site of replication (By similarity).By similarity
Protein 3CD: Is involved in the viral replication complex and viral polypeptide maturation. It exhibits protease activity with a specificity and catalytic efficiency that is different from protease 3C. Protein 3CD lacks polymerase activity. The 3C domain in the context of protein 3CD may have an RNA binding activity (By similarity).By similarity
Protease 3C: cleaves host DDX58/RIG-I and thus contributes to the inhibition of type I interferon production. Cleaves also host PABPC1 (By similarity).By similarity
RNA-directed RNA polymerase: Replicates the viral genomic RNA on the surface of intracellular membranes. May form linear arrays of subunits that propagate along a strong head-to-tail interaction called interface-I. Covalently attaches UMP to a tyrosine of VPg, which is used to prime RNA synthesis. The positive stranded RNA genome is first replicated at virus induced membranous vesicles, creating a dsRNA genomic replication form. This dsRNA is then used as template to synthesize positive stranded RNA genomes. ss+RNA genomes are either translated, replicated or encapsidated (By similarity).PROSITE-ProRule annotation

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation
Selective cleavage of Tyr-|-Gly bond in the picornavirus polyprotein.
Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.
NTP + H2O = NDP + phosphate.

Enzyme regulationi

RNA-directed RNA polymerase: replication or transcription is subject to high level of random mutations by the nucleotide analog ribavirin.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei25 – 251Involved in the interaction with human RTN3
Sitei323 – 3242Cleavage; by Protease 3CSequence Analysis
Active sitei883 – 8831For Protease 2A activityBy similarity
Active sitei901 – 9011For Protease 2A activityBy similarity
Active sitei972 – 9721For Protease 2A activityBy similarity
Sitei1012 – 10132Cleavage; by Protease 3CSequence Analysis
Sitei1440 – 14412Cleavage; by Protease 3CSequence Analysis
Sitei1526 – 15272Cleavage; by Protease 3CSequence Analysis
Sitei1548 – 15492Cleavage; by Protease 3CSequence Analysis
Active sitei1588 – 15881For Protease 3C activitySequence Analysis
Active sitei1619 – 16191For Protease 3C activitySequence Analysis
Active sitei1695 – 16951For Protease 3C activityBy similarity
Sitei1731 – 17322Cleavage; by Protease 3CSequence Analysis
Active sitei2060 – 20601For RdRp activityBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi1240 – 12478ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. cysteine-type endopeptidase activity Source: InterPro
  3. ion channel activity Source: UniProtKB-KW
  4. RNA binding Source: UniProtKB-KW
  5. RNA-directed RNA polymerase activity Source: UniProtKB-KW
  6. RNA helicase activity Source: InterPro
  7. structural molecule activity Source: InterPro

GO - Biological processi

  1. clathrin-mediated endocytosis of virus by host cell Source: UniProtKB-KW
  2. DNA replication Source: UniProtKB-KW
  3. induction by virus of host autophagy Source: UniProtKB
  4. pore formation by virus in membrane of host cell Source: UniProtKB-KW
  5. pore-mediated entry of viral genome into host cell Source: UniProtKB-KW
  6. protein oligomerization Source: UniProtKB-KW
  7. RNA-protein covalent cross-linking Source: UniProtKB-KW
  8. suppression by virus of host gene expression Source: UniProtKB-KW
  9. suppression by virus of host mRNA export from nucleus Source: UniProtKB-KW
  10. suppression by virus of host RIG-I activity by RIG-I proteolysis Source: UniProtKB
  11. suppression by virus of host translation initiation factor activity Source: UniProtKB
  12. transcription, DNA-templated Source: InterPro
  13. viral RNA genome replication Source: InterPro
  14. virion attachment to host cell Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase, Ion channel, Nucleotidyltransferase, Protease, RNA-directed RNA polymerase, Thiol protease, Transferase, Viral ion channel

Keywords - Biological processi

Activation of host autophagy by virus, Clathrin-mediated endocytosis of virus by host, DNA replication, Eukaryotic host gene expression shutoff by virus, Eukaryotic host translation shutoff by virus, Host gene expression shutoff by virus, Host mRNA suppression by virus, Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host mRNA nuclear export by virus, Inhibition of host RIG-I by virus, Inhibition of host RLR pathway by virus, Ion transport, Pore-mediated penetration of viral genome into host cell, Transport, Viral attachment to host cell, Viral immunoevasion, Viral penetration into host cytoplasm, Viral RNA replication, Virus endocytosis by host, Virus entry into host cell

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Genome polyprotein
Cleaved into the following 17 chains:
Alternative name(s):
VP4-VP2
Alternative name(s):
P1A
Virion protein 4
Alternative name(s):
P1B
Virion protein 2
Alternative name(s):
P1C
Virion protein 3
Alternative name(s):
P1D
Virion protein 1
Protease 2A (EC:3.4.22.29)
Short name:
P2A
Alternative name(s):
Picornain 2A
Protein 2A
Protein 2B
Short name:
P2B
Protein 2C (EC:3.6.1.15)
Short name:
P2C
Protein 3A
Short name:
P3A
Alternative name(s):
Protein 3B
Short name:
P3B
Protease 3C (EC:3.4.22.28)
Short name:
P3C
Alternative name(s):
3D polymerase
Short name:
3Dpol
Protein 3D
Short name:
3D
OrganismiHuman enterovirus 71 (strain BrCr) (Ev 71)
Taxonomic identifieri69153 [NCBI]
Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stagePicornaviralesPicornaviridaeEnterovirusEnterovirus A
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000007709: Genome

Subcellular locationi

Chain Capsid protein VP0 : Virion By similarity. Host cytoplasm By similarity
Chain Capsid protein VP2 : Virion By similarity. Host cytoplasm By similarity
Chain Capsid protein VP3 : Virion By similarity. Host cytoplasm By similarity
Chain Capsid protein VP1 : Virion By similarity. Host cytoplasm By similarity
Chain Protein 2B : Host cytoplasmic vesicle membrane Curated; Peripheral membrane protein Curated; Cytoplasmic side Curated
Note: Probably localizes to the surface of intracellular membrane vesicles that are induced after virus infection as the site for viral RNA replication. These vesicles are derived from the endoplasmic reticulum.
Chain Protein 2C : Host cytoplasmic vesicle membrane Curated; Peripheral membrane protein Curated; Cytoplasmic side Curated
Note: Probably localizes to the surface of intracellular membrane vesicles that are induced after virus infection as the site for viral RNA replication. These vesicles are derived from the endoplasmic reticulum.
Chain Protein 3A : Host cytoplasmic vesicle membrane Curated; Peripheral membrane protein Curated; Cytoplasmic side Curated
Note: Probably localizes to the surface of intracellular membrane vesicles that are induced after virus infection as the site for viral RNA replication. These vesicles are derived from the endoplasmic reticulum.
Chain Protein 3AB : Host cytoplasmic vesicle membrane Curated; Peripheral membrane protein Curated; Cytoplasmic side Curated
Note: Probably localizes to the surface of intracellular membrane vesicles that are induced after virus infection as the site for viral RNA replication. These vesicles are derived from the endoplasmic reticulum.
Chain Protein 3CD : Host cytoplasmic vesicle membrane Curated; Peripheral membrane protein Curated; Cytoplasmic side Curated
Note: Probably localizes to the surface of intracellular membrane vesicles that are induced after virus infection as the site for viral RNA replication. These vesicles are derived from the endoplasmic reticulum.
Chain RNA-directed RNA polymerase : Host cytoplasmic vesicle membrane Curated; Peripheral membrane protein Curated; Cytoplasmic side Curated
Note: Probably localizes to the surface of intracellular membrane vesicles that are induced after virus infection as the site for viral RNA replication. These vesicles are derived from the endoplasmic reticulum.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 15031502CytoplasmicSequence AnalysisAdd
BLAST
Intramembranei1504 – 151916Sequence AnalysisAdd
BLAST
Topological domaini1520 – 2193674CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. host cell cytoplasmic vesicle Source: UniProtKB-KW
  2. integral to membrane of host cell Source: UniProtKB-KW
  3. membrane Source: UniProtKB-KW
  4. T=pseudo3 icosahedral viral capsid Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Host cytoplasm, Host cytoplasmic vesicle, Host membrane, Membrane, T=pseudo3 icosahedral capsid protein, Virion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi1121 – 11211D → V: No effect on the interaction with RTN3; when associated with V-1130. 1 Publication
Mutagenesisi1127 – 11271K → T: No effect on the interaction with RTN3; when associated with T-1135. 1 Publication
Mutagenesisi1130 – 11301E → V: No effect on the interaction with RTN3; when associated with E-1121. 1 Publication
Mutagenesisi1135 – 11351K → T: No effect on the interaction with RTN3; when associated with T-1127. 1 Publication
Mutagenesisi1136 – 11361I → K: Loss of interaction with RTN3 and reduced viral cytopathicity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed; by hostBy similarity
Chaini2 – 21932192Genome polyproteinBy similarityPRO_0000426136Add
BLAST
Chaini2 – 862861P1By similarityPRO_0000426137Add
BLAST
Chaini2 – 323322Capsid protein VP0Sequence AnalysisPRO_0000426138Add
BLAST
Chaini2 – 6968Capsid protein VP4Sequence AnalysisPRO_0000426139Add
BLAST
Chaini70 – 323254Capsid protein VP2Sequence AnalysisPRO_0000426140Add
BLAST
Chaini324 – 567244Capsid protein VP3Sequence AnalysisPRO_0000426141Add
BLAST
Chaini568 – 862295Capsid protein VP1Sequence AnalysisPRO_0000426142Add
BLAST
Chaini863 – 1440578P2By similarityPRO_0000426143Add
BLAST
Chaini863 – 1012150Protease 2ASequence AnalysisPRO_0000039485Add
BLAST
Chaini1013 – 111199Protein 2BSequence AnalysisPRO_0000039486Add
BLAST
Chaini1112 – 1440329Protein 2CSequence AnalysisPRO_0000039487Add
BLAST
Chaini1441 – 2193753P3By similarityPRO_0000426144Add
BLAST
Chaini1441 – 1548108Protein 3ABSequence AnalysisPRO_0000426145Add
BLAST
Chaini1441 – 152686Protein 3ASequence AnalysisPRO_0000039488Add
BLAST
Chaini1527 – 154822Viral protein genome-linkedSequence AnalysisPRO_0000426146Add
BLAST
Chaini1549 – 2193645Protein 3CDSequence AnalysisPRO_0000426147Add
BLAST
Chaini1549 – 1730182Protease 3CSequence AnalysisPRO_0000426148Add
BLAST
Chaini1731 – 2193463RNA-directed RNA polymeraseBy similarityPRO_0000426149Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycine; by hostBy similarity
Modified residuei1529 – 15291O-(5'-phospho-RNA)-tyrosineBy similarity

Post-translational modificationi

Specific enzymatic cleavages in vivo by the viral proteases yield a variety of precursors and mature proteins. Polyprotein processing intermediates such as VP0 which is a VP4-VP2 precursor are produced. During virion maturation, non-infectious particles are rendered infectious following cleavage of VP0. This maturation cleavage is followed by a conformational change of the particle (By similarity).By similarity
VPg is uridylylated by the polymerase and is covalently linked to the 5'-end of genomic RNA. This uridylylated form acts as a nucleotide-peptide primer for the polymerase (By similarity).By similarity
Myristoylation of VP4 is required during RNA encapsidation and formation of the mature virus particle.By similarity
Capsid protein VP0: Myristoylation is required for the formation of pentamers during virus assembly. Further assembly of 12 pentamers and a molecule of genomic RNA generates the provirion (By similarity).By similarity
Genome polyprotein: Specific enzymatic cleavages in vivo by the viral proteases yield processing intermediates and the mature proteins.By similarity
Capsid protein VP0: During virion maturation, immature virions are rendered infectious following cleavage of VP0 into VP4 and VP2. This maturation seems to be an autocatalytic event triggered by the presence of RNA in the capsid and it is followed by a conformational change infectious virion (By similarity).By similarity
Viral protein genome-linked: VPg is uridylylated by the polymerase into VPg-pUpU. This acts as a nucleotide-peptide primer for the genomic RNA replication (By similarity).By similarity

Keywords - PTMi

Covalent protein-RNA linkage, Lipoprotein, Myristate, Phosphoprotein

Interactioni

Subunit structurei

Capsid protein VP1: Interacts with capsid protein VP0, and capsid protein VP3 to form heterotrimeric protomers. Five protomers subsequently associate to form pentamers which serve as building blocks for the capsid. Interacts with capsid protein VP4 in the mature capsid (By similarity). Capsid protein VP0: interacts with capsid protein VP1 and capsid protein VP3 to form heterotrimeric protomers. Five protomers subsequently associate to form pentamers which serve as building blocks for the capsid. Capsid protein VP2: Interacts with capsid protein VP1 and capsid protein VP3 in the mature capsid (By similarity). Capsid protein VP3: interacts with capsid protein VP0 and capsid protein VP1 to form heterotrimeric protomers. Five protomers subsequently associate to form pentamers which serve as building blocks for the capsid. Interacts with capsid protein VP4 in the mature capsid (By similarity). Capsid protein VP4: Interacts with capsid protein VP1 and capsid protein VP3 (By similarity). Protein 2C: interacts with capsid protein VP3; this interaction may be important for virion morphogenesis (By similarity). Interacts with human RTN3; this interaction is important for viral replication. Protein 3AB: interacts with protein 3CD (By similarity). Viral protein genome-linked: interacts with RNA-directed RNA polymerase (By similarity). Protein 3CD: interacts with protein 3AB and with RNA-directed RNA polymerase. RNA-directed RNA polymerase: interacts with viral protein genome-linked and with protein 3CD (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ66478.
SMRiQ66478. Positions 2-68, 74-565, 863-1012, 1549-2193.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1216 – 1374159SF3 helicasePROSITE-ProRule annotationAdd
BLAST
Domaini1549 – 1714166Peptidase C3Add
BLAST
Domaini1958 – 2073116RdRp catalyticPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni568 – 58821Amphipatic alpha-helixSequence AnalysisAdd
BLAST
Regioni1441 – 146323DisorderedBy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the picornaviruses polyprotein family.Curated
Contains 1 peptidase C3 domain.Curated
Contains 1 RdRp catalytic domain.PROSITE-ProRule annotation
Contains 1 SF3 helicase domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Family and domain databases

Gene3Di2.60.120.20. 3 hits.
4.10.80.10. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR000605. Helicase_SF3_ssDNA/RNA_vir.
IPR014759. Helicase_SF3_ssRNA_vir.
IPR027417. P-loop_NTPase.
IPR014838. P3A.
IPR000081. Peptidase_C3.
IPR000199. Peptidase_C3A/C3B_picornavir.
IPR003138. Pico_P1A.
IPR002527. Pico_P2B.
IPR001676. Picornavirus_capsid.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009003. Trypsin-like_Pept_dom.
IPR029053. Viral_coat.
[Graphical view]
PfamiPF08727. P3A. 1 hit.
PF00548. Peptidase_C3. 1 hit.
PF02226. Pico_P1A. 1 hit.
PF00947. Pico_P2A. 1 hit.
PF01552. Pico_P2B. 1 hit.
PF00680. RdRP_1. 1 hit.
PF00073. Rhv. 3 hits.
PF00910. RNA_helicase. 1 hit.
[Graphical view]
ProDomiPD001306. Peptidase_C3. 1 hit.
PD649346. Pico_P2B. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 2 hits.
SSF52540. SSF52540. 1 hit.
SSF89043. SSF89043. 1 hit.
PROSITEiPS50507. RDRP_SSRNA_POS. 1 hit.
PS51218. SF3_HELICASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q66478-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGSQVSTQRS GSHENSNSAT EGSTINYTTI NYYKDSYAAT AGKQSLKQDP
60 70 80 90 100
DKFANPVKDI FTEMAAPLKS PSAEACGYSD RVAQLTIGNS TITTQEAANI
110 120 130 140 150
IVGYGEWPSY CSDNDATAVD KPTRPDVSVN RFYTLDTKLW EKSSKGWYWK
160 170 180 190 200
FPDVLTETGV FGPNAQFHYL YRSGFCIHVQ CNASKFHQGA LLVAVLPEYV
210 220 230 240 250
IGTVAGGTGT ENSHPPYKQT QPGADGFELQ HPYVLDAGIP ISQLTVCPHQ
260 270 280 290 300
WINLRTNNCA TIIVPYMNTL PFDSALNHCN FGLLVVPISP LDFDQGATPV
310 320 330 340 350
IPITITLAPM CSEFAGLRQA VTQGFPTELK PGTNQFLTTD DGVSAPILPN
360 370 380 390 400
FHPTPCIHIP GEVRNLLELC QVETILEVNN VPTNATSLME RLRFPVSAQA
410 420 430 440 450
GKGELCAVFR ADPGRDGPWQ STMLGQLCGY YTQWSGSLEV TFMFTGSFMA
460 470 480 490 500
TGKMLIAYTP PGGPLPKDRA TAMLGTHVIW DFGLQSSVTL VIPWISNTHY
510 520 530 540 550
RAHARDGVFD YYTTGLVSIW YQTNYVVPIG APNTAYIIAL AAAQKNFTMK
560 570 580 590 600
LCKDTSDILE TATIQGDRVA DVIESSIGDS VSKALTPALP APTGPDTQVS
610 620 630 640 650
SHRLDTGKVP ALQAAEIGAS SNASDESMIE TRCVLNSHST AETTLDSFFS
660 670 680 690 700
RAGLVGEIDL PLKGTTNPNG YANWDIDITG YAQMRRKVEL FTYMRFDAEF
710 720 730 740 750
TFVACTPTGR VVPQLLQYMF VPPGAPKPDS RDSLAWPTAT NPSVFVKSSD
760 770 780 790 800
PPAQVSVPFM SPASAYQWFY DGYPTFGEHK QEKDLEYGAC PNNMMGTFSV
810 820 830 840 850
RTVGSSKSEY SLVIRIYMRM KHVRAWIPRP MRNQNYLFKS NPNYAGDSIK
860 870 880 890 900
PTGTSRTAIT TLGKFGQQSG AIYVGNFRVV NRHLATHTDW ANLVWEDSSR
910 920 930 940 950
DLLVSSTTAQ GCDTIARCNC QTGVYYCNSR RKHYPVSFSK PSLVFVEASE
960 970 980 990 1000
YYPARYQSHL MLAEGHSEPG DCGGILRCQH GVVGIVSTGG SGLVGFADVR
1010 1020 1030 1040 1050
DLLWLDEEAM EQGVSDYIKG LGRAFGTGFT DAVSREVEAL KNHLIGSEGA
1060 1070 1080 1090 1100
VEKILKNLVK LISALVIVIR SDYDMVTLTA TLALIGCHGS PWAWIKSKTA
1110 1120 1130 1140 1150
SILGIPMAQK QSASWLKKFN DMANAAKGLE WIFNKISKFI DWLKEKIIPA
1160 1170 1180 1190 1200
AKEKVEFLNN LKQLPLLENQ VSNLEQSAAS QEDLEAMFGN VIYLAHFCRK
1210 1220 1230 1240 1250
FQPLYATEAK RVYALEKRMN NYMQFKSKHR IEPVCLIIRG SPGTGKSLAT
1260 1270 1280 1290 1300
GIIARAIADK YRSSVYSLPP DPDHFDGYKQ QVVAVMDDLC QNPDGKDMSL
1310 1320 1330 1340 1350
FCQMVSTVDF VPPMASLEEK GVSFTSKFVI ASTNASNIIV PTVSDSDAIR
1360 1370 1380 1390 1400
RRFYMDCDIE VTDSYKTDLG RLDAGRAAKL CTENNTANFK RCSPLVCGKA
1410 1420 1430 1440 1450
IQLRDRKSKV RYSVDTVVSE LIREYNNRSA IGNTIEALFQ GPPKFRPIRI
1460 1470 1480 1490 1500
SLEEKPAPDA ISDLLASVDS EEVRQYCREQ GWIIPETPTN VERHLNRAVL
1510 1520 1530 1540 1550
VMQSIATVVA VVSLVYVIYK LFAGFQGAYS GAPKPILKKP VLRTATVQGP
1560 1570 1580 1590 1600
SLDFALSLLR RNIRQAQTDQ GHFTMLGVRD RLAILPRHSQ PGKTIWVEHK
1610 1620 1630 1640 1650
LINVLDAVEL VDEQGVNLEL TLVTLDTNEK FRDITKCIPE VITGASDATL
1660 1670 1680 1690 1700
VINTEHIPSM FVPVGDVVQY GFLNLSGKPT HRTMMYNFPT KPGQCGGVVT
1710 1720 1730 1740 1750
SVGKIIGIHI GGNGRQAFCA GLKRSYFASE QGEIQWMKPN RETGRLNING
1760 1770 1780 1790 1800
PTRTKLEPSV FHDVFEGNKE PAVLTSKDPR LEVDFEQALF SKYVGNTLHE
1810 1820 1830 1840 1850
PDEYVTQAAL HYANQLKQLD INTSKMSMEE ACYGTEYLEA IDLHTSAGYP
1860 1870 1880 1890 1900
YSALGIKKRD ILDPVTRDTS RMKLYMDKYG LDLPNSTYVK DELSSLDKIR
1910 1920 1930 1940 1950
KGESRLIEAS SLNDPVYPRL TFGHLYEVFH ANPGTVTGSA VGCNPDVFWS
1960 1970 1980 1990 2000
KLPILLPGSL FAFDYSGYDA SLSPVWFRAL ELVLREIGYS EEAVSLIEGI
2010 2020 2030 2040 2050
NHTHHVYRNK TYCVLGGMPS GCSGTSIFNS MINNIIIRTL LIKTFKGIDL
2060 2070 2080 2090 2100
DELKMVAYGD DVLASYPFPI DCLEWGKTGK EYGLTMTPAD KSPCFNEVTW
2110 2120 2130 2140 2150
ENATFLKRGF LPDHQFPFLI HPTMPMREIH ESIRWTKDAR NTQDHVRSLC
2160 2170 2180 2190
LLAWHNGKEE YEKFVSTIRS VPIGRALAIP NLENLRRNWL ELF
Length:2,193
Mass (Da):242,852
Last modified:January 23, 2007 - v3
Checksum:i0F0A58FB1A35A63F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U22521 Genomic RNA. Translation: AAB39968.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U22521 Genomic RNA. Translation: AAB39968.1 .

3D structure databases

ProteinModelPortali Q66478.
SMRi Q66478. Positions 2-68, 74-565, 863-1012, 1549-2193.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 2.60.120.20. 3 hits.
4.10.80.10. 2 hits.
InterProi IPR003593. AAA+_ATPase.
IPR000605. Helicase_SF3_ssDNA/RNA_vir.
IPR014759. Helicase_SF3_ssRNA_vir.
IPR027417. P-loop_NTPase.
IPR014838. P3A.
IPR000081. Peptidase_C3.
IPR000199. Peptidase_C3A/C3B_picornavir.
IPR003138. Pico_P1A.
IPR002527. Pico_P2B.
IPR001676. Picornavirus_capsid.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009003. Trypsin-like_Pept_dom.
IPR029053. Viral_coat.
[Graphical view ]
Pfami PF08727. P3A. 1 hit.
PF00548. Peptidase_C3. 1 hit.
PF02226. Pico_P1A. 1 hit.
PF00947. Pico_P2A. 1 hit.
PF01552. Pico_P2B. 1 hit.
PF00680. RdRP_1. 1 hit.
PF00073. Rhv. 3 hits.
PF00910. RNA_helicase. 1 hit.
[Graphical view ]
ProDomi PD001306. Peptidase_C3. 1 hit.
PD649346. Pico_P2B. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
SMARTi SM00382. AAA. 1 hit.
[Graphical view ]
SUPFAMi SSF50494. SSF50494. 2 hits.
SSF52540. SSF52540. 1 hit.
SSF89043. SSF89043. 1 hit.
PROSITEi PS50507. RDRP_SSRNA_POS. 1 hit.
PS51218. SF3_HELICASE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete nucleotide sequence of enterovirus 71 is distinct from poliovirus."
    Brown B.A., Pallansch M.A.
    Virus Res. 39:195-206(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Reticulon 3 binds the 2C protein of enterovirus 71 and is required for viral replication."
    Tang W.-F., Yang S.-Y., Wu B.-W., Jheng J.-R., Chen Y.-L., Shih C.-H., Lin K.-H., Lai H.-C., Tang P., Horng J.-T.
    J. Biol. Chem. 282:5888-5898(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH HUMAN RTN3, MUTAGENESIS OF ASP-1121; LYS-1127; GLU-1130; LYS-1135 AND ILE-1136.
  3. "The essential role of clathrin-mediated endocytosis in the infectious entry of human enterovirus 71."
    Hussain K.M., Leong K.L., Ng M.M., Chu J.J.
    J. Biol. Chem. 286:309-321(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF CAPSID PROTEINS.
    Strain: H.

Entry informationi

Entry nameiPOLG_HE71B
AccessioniPrimary (citable) accession number: Q66478
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 131 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3